This is a Validated Antibody Database (VAD) review about mouse Podxl, based on 21 published articles (read how Labome selects the articles), using Podxl antibody in all methods. It is aimed to help Labome visitors find the most suited Podxl antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Podxl synonym: AW121214; Ly102; PCLP-1; Pclp1; Podxl1; podocalyxin; podocalyxin-like protein 1

Knockout validation
R&D Systems
goat polyclonal
  • immunocytochemistry knockout validation; mouse; fig 1c
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1c
  • western blot knockout validation; mouse; fig s3b
In order to characterize podocalyxin conditional knockout mice and study endothelial cells, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunocytochemistry knockout validation on mouse samples (fig 1c), in immunohistochemistry knockout validation on mouse samples (fig 1c) and in western blot knockout validation on mouse samples (fig s3b). Eur J Cell Biol (2016) ncbi
R&D Systems
goat polyclonal
  • immunocytochemistry knockout validation; human; 1:500; fig 5
  • western blot knockout validation; human; 1:500; fig 5
In order to create and characterize a model for kidney disease using CRISPR-mutants, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunocytochemistry knockout validation on human samples at 1:500 (fig 5) and in western blot knockout validation on human samples at 1:500 (fig 5). Nat Commun (2015) ncbi
R&D Systems
goat polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig s3g
R&D Systems Podxl antibody (R&D, AF1556) was used in immunohistochemistry on mouse samples at 1:200 (fig s3g). Nat Commun (2019) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig s1b
R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig s1b). Cell (2018) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4c
In order to investigate the role of inositol-requiring enzyme-1 alpha in glomerular capillary integrity and autophagy in podocytes, R&D Systems Podxl antibody (R&D, AF1556) was used in immunohistochemistry - paraffin section on mouse samples (fig 4c). Mol Biol Cell (2017) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1a
In order to characterize 3 transgenic mouse lines in which different platelet-derived growth factor ligands are expressed under the same heart specific promoter, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - paraffin section on mouse samples (fig 1a). Exp Cell Res (2016) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2a
In order to study the interaction between ALK1 and endoglin in pancreatic neuroendocrine tumors, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2a). Oncotarget (2016) ncbi
rat monoclonal (192703)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4d
In order to show that Endoglycan is a pre-luminal marker, R&D Systems Podxl antibody (R&D, MAB1556) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4d). Dev Biol (2016) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s1c
In order to explore the impact of Notch1 signaling on the development of clear cell renal cell carcinoma, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - paraffin section on mouse samples (fig s1c). Sci Rep (2016) ncbi
goat polyclonal
  • immunocytochemistry knockout validation; mouse; fig 1c
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1c
  • western blot knockout validation; mouse; fig s3b
In order to characterize podocalyxin conditional knockout mice and study endothelial cells, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunocytochemistry knockout validation on mouse samples (fig 1c), in immunohistochemistry knockout validation on mouse samples (fig 1c) and in western blot knockout validation on mouse samples (fig s3b). Eur J Cell Biol (2016) ncbi
goat polyclonal
  • immunohistochemistry; mouse; loading ...; fig 3a
  • western blot; mouse; loading ...; fig 4a
In order to use knockout mice to study the involvement of iPLA2 gamma in glomerular pathophysiology, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry on mouse samples (fig 3a) and in western blot on mouse samples (fig 4a). J Biol Chem (2016) ncbi
goat polyclonal
  • flow cytometry; mouse; loading ...; fig 5a
  • western blot; mouse; loading ...; fig 5d
In order to evaluate the 3D3 PODXL antibody for the elimination of tumorigenic pluripotent cells, R&D Systems Podxl antibody (R&D, AF1556) was used in flow cytometry on mouse samples (fig 5a) and in western blot on mouse samples (fig 5d). Stem Cells Dev (2016) ncbi
goat polyclonal
  • immunohistochemistry - free floating section; mouse; fig 1
In order to characterize the rearrangement in the cerebroventricular malformation and cerebrovascular tree due to a tPA deficiency in mice, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - free floating section on mouse samples (fig 1). Front Cell Neurosci (2015) ncbi
goat polyclonal
  • immunocytochemistry knockout validation; human; 1:500; fig 5
  • western blot knockout validation; human; 1:500; fig 5
In order to create and characterize a model for kidney disease using CRISPR-mutants, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunocytochemistry knockout validation on human samples at 1:500 (fig 5) and in western blot knockout validation on human samples at 1:500 (fig 5). Nat Commun (2015) ncbi
goat polyclonal
  • immunocytochemistry; mouse; 1:200; fig 6
  • western blot; mouse; 1:1000; fig 6
In order to elucidate the role of IFT proteins in microtubule stabilization after discovery of mutations in TRAF3IP1/IFT54, R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunocytochemistry on mouse samples at 1:200 (fig 6) and in western blot on mouse samples at 1:1000 (fig 6). Nat Commun (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500
In order to characterize the anti-tumor effects of OXA-11, R&D Systems Podxl antibody (R&D Systems, #AF1556) was used in immunohistochemistry - frozen section on mouse samples at 1:500. Clin Exp Metastasis (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200
In order to study the contribution of protease tissue plasminogen activator to seizures, R&D Systems Podxl antibody (R&D systems, AF1556) was used in immunohistochemistry - frozen section on mouse samples at 1:200. Ann Clin Transl Neurol (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; fig s1q-s
R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - frozen section on mouse samples (fig s1q-s). Development (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100
R&D Systems Podxl antibody (R&D Systems, AF1556) was used in immunohistochemistry - frozen section on mouse samples at 1:100. Cancer Res (2015) ncbi
goat polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s1b
R&D Systems Podxl antibody (R&D, AF1556) was used in western blot on mouse samples at 1:1000 (fig s1b). Nat Med (2015) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:50
In order to study blood vessel polarization during angiogenic sprouting, R&D Systems Podxl antibody (R & D Systems, AF1556) was used in immunohistochemistry on mouse samples at 1:50. Development (2014) ncbi
rat monoclonal (192703)
  • flow cytometry; mouse; 1:10
R&D Systems Podxl antibody (R&D, FAB1556P) was used in flow cytometry on mouse samples at 1:10. Stem Cells (2014) ncbi
rat monoclonal (192703)
  • immunohistochemistry; mouse; 1:40; fig 7
In order to investigate the role of cadherin regulator p120 catenin to the development of kidney tubules, R&D Systems Podxl antibody (R&D, MAB1556) was used in immunohistochemistry on mouse samples at 1:40 (fig 7). Development (2011) ncbi
Articles Reviewed
  1. Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  2. Dias D, Kim H, Holl D, Werne Solnestam B, Lundeberg J, Carlen M, et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell. 2018;173:153-165.e22 pubmed publisher
  3. Kaufman D, Papillon J, Larose L, Iwawaki T, Cybulsky A. Deletion of inositol-requiring enzyme-1? in podocytes disrupts glomerular capillary integrity and autophagy. Mol Biol Cell. 2017;28:1636-1651 pubmed publisher
  4. Gallini R, Lindblom P, Bondjers C, Betsholtz C, Andrae J. PDGF-A and PDGF-B induces cardiac fibrosis in transgenic mice. Exp Cell Res. 2016;349:282-290 pubmed publisher
  5. Eleftheriou N, Sjölund J, Bocci M, Cortez E, Lee S, Cunha S, et al. Compound genetically engineered mouse models of cancer reveal dual targeting of ALK1 and endoglin as a synergistic opportunity to impinge on angiogenic TGF-β signaling. Oncotarget. 2016;7:84314-84325 pubmed publisher
  6. Yang Z, Zimmerman S, Tsunezumi J, Braitsch C, Trent C, Bryant D, et al. Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016;418:66-74 pubmed publisher
  7. Johansson E, Rönö B, Johansson M, Lindgren D, Möller C, Axelson H, et al. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice. Sci Rep. 2016;6:30739 pubmed publisher
  8. Horrillo A, Porras G, Ayuso M, González Manchón C. Loss of endothelial barrier integrity in mice with conditional ablation of podocalyxin (Podxl) in endothelial cells. Eur J Cell Biol. 2016;95:265-76 pubmed publisher
  9. Elimam H, Papillon J, Kaufman D, Guillemette J, Aoudjit L, Gross R, et al. Genetic Ablation of Calcium-independent Phospholipase A2? Induces Glomerular Injury in Mice. J Biol Chem. 2016;291:14468-82 pubmed publisher
  10. Kang L, Yao C, Khodadadi Jamayran A, Xu W, Zhang R, Banerjee N, et al. The Universal 3D3 Antibody of Human PODXL Is Pluripotent Cytotoxic, and Identifies a Residual Population After Extended Differentiation of Pluripotent Stem Cells. Stem Cells Dev. 2016;25:556-68 pubmed publisher
  11. Stefanitsch C, Lawrence A, Olverling A, Nilsson I, Fredriksson L. tPA Deficiency in Mice Leads to Rearrangement in the Cerebrovascular Tree and Cerebroventricular Malformations. Front Cell Neurosci. 2015;9:456 pubmed publisher
  12. Freedman B, Brooks C, Lam A, Fu H, Morizane R, Agrawal V, et al. Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nat Commun. 2015;6:8715 pubmed publisher
  13. Bizet A, Becker Heck A, Ryan R, Weber K, Filhol E, Krug P, et al. Mutations in TRAF3IP1/IFT54 reveal a new role for IFT proteins in microtubule stabilization. Nat Commun. 2015;6:8666 pubmed publisher
  14. Moen I, Gebre M, Alonso Camino V, Chen D, Epstein D, McDonald D. Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors. Clin Exp Metastasis. 2015;32:799-817 pubmed publisher
  15. Fredriksson L, Stevenson T, Su E, Ragsdale M, Moore S, Craciun S, et al. Identification of a neurovascular signaling pathway regulating seizures in mice. Ann Clin Transl Neurol. 2015;2:722-38 pubmed publisher
  16. Hurtado R, Zewdu R, Mtui J, Liang C, Aho R, Kurylo C, et al. Pbx1-dependent control of VMC differentiation kinetics underlies gross renal vascular patterning. Development. 2015;142:2653-64 pubmed publisher
  17. Cunha S, Bocci M, Lövrot J, Eleftheriou N, Roswall P, Cordero E, et al. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer. Cancer Res. 2015;75:2445-56 pubmed publisher
  18. Schiffer M, Teng B, Gu C, Shchedrina V, Kasaikina M, Pham V, et al. Pharmacological targeting of actin-dependent dynamin oligomerization ameliorates chronic kidney disease in diverse animal models. Nat Med. 2015;21:601-9 pubmed publisher
  19. Pelton J, Wright C, Leitges M, Bautch V. Multiple endothelial cells constitute the tip of developing blood vessels and polarize to promote lumen formation. Development. 2014;141:4121-6 pubmed publisher
  20. Zhang H, Nieves J, Fraser S, Isern J, Douvaras P, Papatsenko D, et al. Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm. Stem Cells. 2014;32:191-203 pubmed publisher
  21. MARCIANO D, BRAKEMAN P, Lee C, Spivak N, Eastburn D, Bryant D, et al. p120 catenin is required for normal renal tubulogenesis and glomerulogenesis. Development. 2011;138:2099-109 pubmed publisher