This is a Validated Antibody Database (VAD) review about mouse Phb2, based on 10 published articles (read how Labome selects the articles), using Phb2 antibody in all methods. It is aimed to help Labome visitors find the most suited Phb2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Phb2 synonym: AU044498; BAP; Bap37; Bcap37; REA; prohibitin-2; B-cell receptor-associated protein 37; B-cell receptor-associated protein BAP37; phb2; repressor of estrogen receptor activity

Knockout validation
Invitrogen
rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig 3c
  • immunocytochemistry; human; 1:500; loading ...; fig 4a
  • western blot; human; 1:500; loading ...; fig 1e
  • western blot knockout validation; mouse; 1:500; loading ...; fig 2h
In order to recognize the inner mitochondrial membrane protein, prohibitin 2, as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation, Invitrogen Phb2 antibody (ThermoFisher Scientific, PA5-14133) was used in proximity ligation assay on human samples (fig 3c), in immunocytochemistry on human samples at 1:500 (fig 4a), in western blot on human samples at 1:500 (fig 1e) and in western blot knockout validation on mouse samples at 1:500 (fig 2h). Cell (2017) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; loading ...; fig 6b
  • western blot knockout validation; mouse; loading ...; fig 1c
EMD Millipore Phb2 antibody (Millipore, 07-234) was used in western blot on human samples (fig 6b) and in western blot knockout validation on mouse samples (fig 1c). Oncotarget (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-2)
  • immunoprecipitation; human; 1:100; loading ...; fig 1b
In order to recognize the inner mitochondrial membrane protein, prohibitin 2, as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation, Santa Cruz Biotechnology Phb2 antibody (Santa Cruz, sc-133094) was used in immunoprecipitation on human samples at 1:100 (fig 1b). Cell (2017) ncbi
mouse monoclonal (A-2)
  • immunohistochemistry - paraffin section; human; 1:50; fig 1c
Santa Cruz Biotechnology Phb2 antibody (Santa Cruz, sc- 133094) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1c). Turk J Gastroenterol (2016) ncbi
mouse monoclonal (II-14-10)
  • western blot; rat
In order to identify the role of mKATP channel activation in mitochondrial membrane potential and cell death, Santa Cruz Biotechnology Phb2 antibody (Santa Cruz, sc-56346) was used in western blot on rat samples . PLoS ONE (2014) ncbi
mouse monoclonal (II-14-10)
  • western blot; rat
  • western blot; human
In order to examine the mechanism of translocation in regards to glycogen synthase kinase-3 protein, Santa Cruz Biotechnology Phb2 antibody (Santa Cruz Biotechnology, sc-56346) was used in western blot on rat samples and in western blot on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (A-2)
  • western blot; mouse
In order to study the role of Runx1 and PU.1 in corepressor-coactivator exchange and the potential role of PU.1-recruited corepressors as targets for leukemia differentiation therapy, Santa Cruz Biotechnology Phb2 antibody (Santa Cruz Biotechnology, sc-133094) was used in western blot on mouse samples . J Biol Chem (2014) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry; rat; 1:100; fig 7d
In order to identify novel molecules in peripheral myelination by spatial mapping of juxtacrine axo-glial interactions, Proteintech Group Phb2 antibody (Proteintech, 12295-1-AP) was used in immunohistochemistry on rat samples at 1:100 (fig 7d). Nat Commun (2015) ncbi
BioLegend
rabbit polyclonal (Poly6118)
  • western blot; human; fig 2e
BioLegend Phb2 antibody (BioLegend, 611802) was used in western blot on human samples (fig 2e). Hum Mol Genet (2016) ncbi
Invitrogen
rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig 3c
  • immunocytochemistry; human; 1:500; loading ...; fig 4a
  • western blot; human; 1:500; loading ...; fig 1e
  • western blot knockout validation; mouse; 1:500; loading ...; fig 2h
In order to recognize the inner mitochondrial membrane protein, prohibitin 2, as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation, Invitrogen Phb2 antibody (ThermoFisher Scientific, PA5-14133) was used in proximity ligation assay on human samples (fig 3c), in immunocytochemistry on human samples at 1:500 (fig 4a), in western blot on human samples at 1:500 (fig 1e) and in western blot knockout validation on mouse samples at 1:500 (fig 2h). Cell (2017) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; fig 2
Bethyl Phb2 antibody (Bethyl Lab, A300-657A) was used in western blot on human samples (fig 2). Biochim Biophys Acta (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; loading ...; fig 5b,5c,6b,6c,6d
EMD Millipore Phb2 antibody (Millipore, 07-234) was used in western blot on human samples (fig 5b,5c,6b,6c,6d). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 6b
  • western blot knockout validation; mouse; loading ...; fig 1c
EMD Millipore Phb2 antibody (Millipore, 07-234) was used in western blot on human samples (fig 6b) and in western blot knockout validation on mouse samples (fig 1c). Oncotarget (2015) ncbi
Articles Reviewed
  1. Wei Y, Chiang W, Sumpter R, Mishra P, Levine B. Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor. Cell. 2017;168:224-238.e10 pubmed publisher
  2. Pomares H, Palmeri C, Iglesias Serret D, Moncunill Massaguer C, Saura Esteller J, Núñez Vázquez S, et al. Targeting prohibitins induces apoptosis in acute myeloid leukemia cells. Oncotarget. 2016;7:64987-65000 pubmed publisher
  3. Kucuk I, Tanoglu A, Oncu K, Yilmaz I, Kara M, Beyazit Y, et al. Immunohistochemical activity of Prohibitin-2 and Stomatin-Like Protein-2 in patients with ulcerative colitis. Turk J Gastroenterol. 2016;27:233-8 pubmed publisher
  4. Lu Y, Galbraith L, Herndon J, Lü Y, Pras Raves M, Vervaart M, et al. Defining functional classes of Barth syndrome mutation in humans. Hum Mol Genet. 2016;25:1754-70 pubmed publisher
  5. Moncunill Massaguer C, Saura Esteller J, Pérez Perarnau A, Palmeri C, Núñez Vázquez S, Cosialls A, et al. A novel prohibitin-binding compound induces the mitochondrial apoptotic pathway through NOXA and BIM upregulation. Oncotarget. 2015;6:41750-65 pubmed publisher
  6. Poitelon Y, Bogni S, Matafora V, Della Flora Nunes G, Hurley E, Ghidinelli M, et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun. 2015;6:8303 pubmed publisher
  7. Sun L, Hartson S, Matts R. Identification of proteins associated with Aha1 in HeLa cells by quantitative proteomics. Biochim Biophys Acta. 2015;1854:365-80 pubmed publisher
  8. Sunaga D, Tanno M, Kuno A, Ishikawa S, Ogasawara M, Yano T, et al. Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis. PLoS ONE. 2014;9:e112529 pubmed publisher
  9. Tanno M, Kuno A, Ishikawa S, Miki T, Kouzu H, Yano T, et al. Translocation of glycogen synthase kinase-3β (GSK-3β), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2). J Biol Chem. 2014;289:29285-96 pubmed publisher
  10. Gu X, Hu Z, Ebrahem Q, Crabb J, Mahfouz R, Radivoyevitch T, et al. Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. J Biol Chem. 2014;289:14881-95 pubmed publisher