This is a Validated Antibody Database (VAD) review about mouse Pcnt, based on 35 published articles (read how Labome selects the articles), using Pcnt antibody in all methods. It is aimed to help Labome visitors find the most suited Pcnt antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Pcnt synonym: AW476095; C86676; KEN; Pcnt2; kendrin; m239Asp; m275Asp

Knockout validation
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; human; 1:1000; fig s3
Abcam Pcnt antibody (Abcam, Ab4448) was used in immunohistochemistry knockout validation on human samples at 1:1000 (fig s3). PLoS ONE (2016) ncbi
Abcam
mouse monoclonal (mAbcam 28144)
  • immunocytochemistry; human; 1:400; loading ...; fig 4b
  • western blot; human; 1:1000; loading ...; fig 4a
Abcam Pcnt antibody (Abcam, 28144) was used in immunocytochemistry on human samples at 1:400 (fig 4b) and in western blot on human samples at 1:1000 (fig 4a). Nat Microbiol (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig s4i
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples at 1:1000 (fig s4i). Science (2018) ncbi
mouse monoclonal (mAbcam 28144)
  • immunohistochemistry; mouse; fig ev4d
Abcam Pcnt antibody (Abcam, ab28144) was used in immunohistochemistry on mouse samples (fig ev4d). EMBO Rep (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 2d
In order to Investigate the regulation of GABARAP ubiquitination and GABARAP-mediated autophagy, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 2d). Curr Biol (2017) ncbi
domestic rabbit polyclonal
  • other; human; 1:200; loading ...; fig s1a
  • immunocytochemistry; human; 1:2000; loading ...; fig 1a, 6a
Abcam Pcnt antibody (Abcam, ab4448) was used in other on human samples at 1:200 (fig s1a) and in immunocytochemistry on human samples at 1:2000 (fig 1a, 6a). Nat Commun (2017) ncbi
mouse monoclonal (mAbcam 28144)
  • immunocytochemistry; human; 1:200; loading ...; fig s4a
  • western blot; human; loading ...; fig s4b
In order to show that USP9X regulates centrosome biogenesis and plays a role breast carcinogenesis, Abcam Pcnt antibody (Abcam, ab28144) was used in immunocytochemistry on human samples at 1:200 (fig s4a) and in western blot on human samples (fig s4b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 2d
Abcam Pcnt antibody (Abcam, Ab4448) was used in immunocytochemistry on human samples (fig 2d). Cell Stem Cell (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 8c
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 8c). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:3000; loading ...; fig s14
In order to identify MAPKBP1 mutations as a genetic cause of juvenile or late-onset and cilia-independent nephronophthisis, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples at 1:3000 (fig s14). Am J Hum Genet (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 9b
In order to demonstrate that INPP5E regulates cell division, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 9b). Mol Cell Biol (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:5000; loading ...; fig s1a
In order to elucidate tumor-derived factors and genetic changes in endothelial cells that contribute to excess centrosomes in tumor endothelial cells, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples at 1:5000 (fig s1a). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:300; loading ...; fig 6a
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples at 1:300 (fig 6a). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:400; fig s1
  • immunocytochemistry; human; 1:400; fig s5
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on mouse samples at 1:400 (fig s1) and in immunocytochemistry on human samples at 1:400 (fig s5). BMC Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 3a
In order to study the capacity of Fatostatin to inhibit sterol regulatory element-binding protein activity and cell division, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 3a). J Biol Chem (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; fig 4
In order to identify pericentrin as a novel regulator of piezo2 in somatosensory neurons by native piezo2 interactomics, Abcam Pcnt antibody (Abcam, ab4448) was used in immunohistochemistry on mouse samples at 1:500 (fig 4). J Proteome Res (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; fig 2
In order to study functionally distinct gamma-tubulin complexes and divergent regulation during differentiation, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on mouse samples (fig 2). J Cell Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 5a
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 5a). EMBO Rep (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:1000; fig 2
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples at 1:1000 (fig 2). Mol Biol Cell (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 1
Abcam Pcnt antibody (Abcam, Ab4448) was used in immunocytochemistry on human samples (fig 1). Mol Biol Cell (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 5
In order to analyze programming of viral genomes for specific cytoplasmic trafficking behaviors by HIV-1 and M-PMV RNA nuclear export elements, Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 5). PLoS Pathog (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 3
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 3). Cell Rep (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 2
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 2). J Cell Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 3
Abcam Pcnt antibody (Abcam, ab44448) was used in immunocytochemistry on human samples (fig 3). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 6
  • western blot; human; fig 6
Abcam Pcnt antibody (Abcam, ab4448) was used in immunocytochemistry on human samples (fig 6) and in western blot on human samples (fig 6). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; fig 2
In order to elucidate the requirement for atypical protein kinase c-dependent polarized cell division in myocardial trabeculation, Abcam Pcnt antibody (Abcam, ab4448) was used in immunohistochemistry - frozen section on mouse samples (fig 2). Cell Rep (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; human; 1:1000; fig s3
Abcam Pcnt antibody (Abcam, Ab4448) was used in immunohistochemistry knockout validation on human samples at 1:1000 (fig s3). PLoS ONE (2016) ncbi
BD Biosciences
mouse monoclonal (30/Pericentrin)
  • immunohistochemistry - frozen section; mouse; 1:2000; loading ...; fig 1a
BD Biosciences Pcnt antibody (BD Biosciences, 611814) was used in immunohistochemistry - frozen section on mouse samples at 1:2000 (fig 1a). elife (2019) ncbi
mouse monoclonal (30/Pericentrin)
  • immunohistochemistry; mouse; 1:200; loading ...; fig s3c
In order to search for the cue that drives blastocyst embryonic lineage segregation, BD Biosciences Pcnt antibody (BD, 611814) was used in immunohistochemistry on mouse samples at 1:200 (fig s3c). Dev Cell (2017) ncbi
mouse monoclonal (30/Pericentrin)
  • immunocytochemistry; mouse; 1:100; loading ...; fig s1
In order to determine the role of haspin in bipolar spindle assembly, BD Biosciences Pcnt antibody (BD Biosciences, 611814) was used in immunocytochemistry on mouse samples at 1:100 (fig s1). J Cell Sci (2016) ncbi
mouse monoclonal (30/Pericentrin)
  • immunocytochemistry; human; 1:400; fig s5
  • immunocytochemistry; mouse; 1:400; fig s1
BD Biosciences Pcnt antibody (BD Biosciences, 611815) was used in immunocytochemistry on human samples at 1:400 (fig s5) and in immunocytochemistry on mouse samples at 1:400 (fig s1). BMC Biol (2016) ncbi
mouse monoclonal (30/Pericentrin)
  • immunocytochemistry; mouse; 1:200; loading ...; fig 3b
In order to assess the role of Setdb1 in oogenesis and pre-implantation development, BD Biosciences Pcnt antibody (BD Transduction Laboratories, 611814) was used in immunocytochemistry on mouse samples at 1:200 (fig 3b). Development (2016) ncbi
mouse monoclonal (30/Pericentrin)
  • immunocytochemistry; mouse; fig 4
In order to use knockout mice to determine the function of RPGRIP1L, BD Biosciences Pcnt antibody (BD, 611814) was used in immunocytochemistry on mouse samples (fig 4). J Cell Biol (2015) ncbi
mouse monoclonal (30/Pericentrin)
  • immunohistochemistry; mouse; 1:750; fig s1
BD Biosciences Pcnt antibody (BD Biosciences, 611815) was used in immunohistochemistry on mouse samples at 1:750 (fig s1). Nat Commun (2015) ncbi
mouse monoclonal (30/Pericentrin)
  • immunohistochemistry - frozen section; mouse; 1:200
BD Biosciences Pcnt antibody (BD Biosciences, 611815) was used in immunohistochemistry - frozen section on mouse samples at 1:200. J Comp Neurol (2012) ncbi
EMD Millipore
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:500; fig 3a
EMD Millipore Pcnt antibody (Millipore, ABT59) was used in immunocytochemistry on human samples at 1:500 (fig 3a). PLoS ONE (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
EMD Millipore Pcnt antibody (Millipore, ABT59) was used in western blot on human samples (fig 2a). J Biol Chem (2017) ncbi
Articles Reviewed
  1. Bendriem R, Singh S, Aleem A, Antonetti D, Ross M. Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. elife. 2019;8: pubmed publisher
  2. Full F, van Gent M, Sparrer K, Chiang C, Zurenski M, Scherer M, et al. Centrosomal protein TRIM43 restricts herpesvirus infection by regulating nuclear lamina integrity. Nat Microbiol. 2019;4:164-176 pubmed publisher
  3. Gut G, Herrmann M, Pelkmans L. Multiplexed protein maps link subcellular organization to cellular states. Science. 2018;361: pubmed publisher
  4. Hsieh W, Ramadesikan S, FEKETE D, Aguilar R. Kidney-differentiated cells derived from Lowe Syndrome patient's iPSCs show ciliogenesis defects and Six2 retention at the Golgi complex. PLoS ONE. 2018;13:e0192635 pubmed publisher
  5. Vogl C, Butola T, Haag N, Hausrat T, Leitner M, Moutschen M, et al. The BEACH protein LRBA is required for hair bundle maintenance in cochlear hair cells and for hearing. EMBO Rep. 2017;18:2015-2029 pubmed publisher
  6. Joachim J, Razi M, Judith D, Wirth M, Calamita E, Encheva V, et al. Centriolar Satellites Control GABARAP Ubiquitination and GABARAP-Mediated Autophagy. Curr Biol. 2017;27:2123-2136.e7 pubmed publisher
  7. Wang Q, Tang Y, Xu Y, Xu S, Jiang Y, Dong Q, et al. The X-linked deubiquitinase USP9X is an integral component of centrosome. J Biol Chem. 2017;292:12874-12884 pubmed publisher
  8. Morris E, Kawamura E, Gillespie J, Balgi A, Kannan N, Muller W, et al. Stat3 regulates centrosome clustering in cancer cells via Stathmin/PLK1. Nat Commun. 2017;8:15289 pubmed publisher
  9. Li X, Song N, Liu L, Liu X, Ding X, Song X, et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun. 2017;8:14866 pubmed publisher
  10. Korotkevich E, Niwayama R, Courtois A, Friese S, Berger N, Buchholz F, et al. The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo. Dev Cell. 2017;40:235-247.e7 pubmed publisher
  11. Bershteyn M, Nowakowski T, Pollen A, Di Lullo E, Nene A, Wynshaw Boris A, et al. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia. Cell Stem Cell. 2017;20:435-449.e4 pubmed publisher
  12. Malecki J, Aileni V, Ho A, Schwarz J, Moen A, Sørensen V, et al. The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). Nucleic Acids Res. 2017;45:4370-4389 pubmed publisher
  13. Macia M, Halbritter J, Delous M, Bredrup C, Gutter A, Filhol E, et al. Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis. Am J Hum Genet. 2017;100:323-333 pubmed publisher
  14. Sierra Potchanant E, Cerabona D, Sater Z, He Y, Sun Z, Gehlhausen J, et al. INPP5E Preserves Genomic Stability through Regulation of Mitosis. Mol Cell Biol. 2017;37: pubmed publisher
  15. Yu Z, Mouillesseaux K, Kushner E, Bautch V. Tumor-Derived Factors and Reduced p53 Promote Endothelial Cell Centrosome Over-Duplication. PLoS ONE. 2016;11:e0168334 pubmed publisher
  16. Yu F, Sharma S, Skowronek A, Erdmann K. The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis. Sci Rep. 2016;6:35399 pubmed publisher
  17. Balboula A, Nguyen A, Gentilello A, Quartuccio S, Drutovic D, Solc P, et al. Haspin kinase regulates microtubule-organizing center clustering and stability through Aurora kinase C in mouse oocytes. J Cell Sci. 2016;129:3648-3660 pubmed
  18. Pourcelot M, Zemirli N, Silva da Costa L, Loyant R, Garcin D, Vitour D, et al. The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing. BMC Biol. 2016;14:69 pubmed publisher
  19. Gholkar A, Cheung K, Williams K, Lo Y, Hamideh S, Nnebe C, et al. Fatostatin Inhibits Cancer Cell Proliferation by Affecting Mitotic Microtubule Spindle Assembly and Cell Division. J Biol Chem. 2016;291:17001-8 pubmed publisher
  20. Narayanan P, Sondermann J, Rouwette T, Karaca S, Urlaub H, Mitkovski M, et al. Native Piezo2 Interactomics Identifies Pericentrin as a Novel Regulator of Piezo2 in Somatosensory Neurons. J Proteome Res. 2016;15:2676-87 pubmed publisher
  21. Eymery A, Liu Z, Ozonov E, Stadler M, Peters A. The methyltransferase Setdb1 is essential for meiosis and mitosis in mouse oocytes and early embryos. Development. 2016;143:2767-79 pubmed publisher
  22. Muroyama A, Seldin L, Lechler T. Divergent regulation of functionally distinct γ-tubulin complexes during differentiation. J Cell Biol. 2016;213:679-92 pubmed publisher
  23. Sánchez A, Urrego D, Pardo L. Cyclic expression of the voltage-gated potassium channel KV10.1 promotes disassembly of the primary cilium. EMBO Rep. 2016;17:708-23 pubmed publisher
  24. Kushner E, Ferro L, Yu Z, Bautch V. Excess centrosomes perturb dynamic endothelial cell repolarization during blood vessel formation. Mol Biol Cell. 2016;27:1911-20 pubmed publisher
  25. Nivon M, Fort L, Muller P, Richet E, Simon S, Guey B, et al. NF?B is a central regulator of protein quality control in response to protein aggregation stresses via autophagy modulation. Mol Biol Cell. 2016;27:1712-27 pubmed publisher
  26. Pocock G, Becker J, Swanson C, Ahlquist P, Sherer N. HIV-1 and M-PMV RNA Nuclear Export Elements Program Viral Genomes for Distinct Cytoplasmic Trafficking Behaviors. PLoS Pathog. 2016;12:e1005565 pubmed publisher
  27. Huang C, Cheng J, Bawa Khalfe T, Yao X, Chin Y, Yeh E. SUMOylated ORC2 Recruits a Histone Demethylase to Regulate Centromeric Histone Modification and Genomic Stability. Cell Rep. 2016;15:147-157 pubmed publisher
  28. Zhao W, Liu J, Zhang X, Deng L. MLL5 maintains spindle bipolarity by preventing aberrant cytosolic aggregation of PLK1. J Cell Biol. 2016;212:829-43 pubmed publisher
  29. Lasek A, McPherson B, Trueman N, Burkard M. The Functional Significance of Posttranslational Modifications on Polo-Like Kinase 1 Revealed by Chemical Genetic Complementation. PLoS ONE. 2016;11:e0150225 pubmed publisher
  30. Maine E, Westcott J, Prechtl A, Dang T, Whitehurst A, Pearson G. The cancer-testis antigens SPANX-A/C/D and CTAG2 promote breast cancer invasion. Oncotarget. 2016;7:14708-26 pubmed publisher
  31. Passer D, van de Vrugt A, Atmanli A, Domian I. Atypical Protein Kinase C-Dependent Polarized Cell Division Is Required for Myocardial Trabeculation. Cell Rep. 2016;14:1662-1672 pubmed publisher
  32. Czub B, Shah A, Alfano G, Kruczek P, Chakarova C, Bhattacharya S. TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene. PLoS ONE. 2016;11:e0148678 pubmed publisher
  33. Gerhardt C, Lier J, Burmühl S, Struchtrup A, Deutschmann K, Vetter M, et al. The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium. J Cell Biol. 2015;210:115-33 pubmed publisher
  34. Clift D, Schuh M. A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes. Nat Commun. 2015;6:7217 pubmed publisher
  35. Arellano J, Guadiana S, Breunig J, Rakic P, Sarkisian M. Development and distribution of neuronal cilia in mouse neocortex. J Comp Neurol. 2012;520:848-73 pubmed publisher