This is a Validated Antibody Database (VAD) review about mouse Pax5, based on 16 published articles (read how Labome selects the articles), using Pax5 antibody in all methods. It is aimed to help Labome visitors find the most suited Pax5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Pax5 synonym: BSAP; EBB-1; KLP; Pax-5

Santa Cruz Biotechnology
mouse monoclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 7b
Santa Cruz Biotechnology Pax5 antibody (Santa Cruz Biotechnology, A-11) was used in chromatin immunoprecipitation on mouse samples (fig 7b). PLoS Genet (2017) ncbi
mouse monoclonal (A-11)
  • chromatin immunoprecipitation; mouse; loading ...; fig 7b
Santa Cruz Biotechnology Pax5 antibody (Santa Cruz Biotechnology, A-11) was used in chromatin immunoprecipitation on mouse samples (fig 7b). PLoS Genet (2017) ncbi
mouse monoclonal (A-11)
  • western blot; mouse; loading ...; fig s8e
  • western blot; human; loading ...; fig 6e
In order to explore transcriptional regulation mediated by p27, Pax5, and PCAF, Santa Cruz Biotechnology Pax5 antibody (Santa Cruz, sc-13146) was used in western blot on mouse samples (fig s8e) and in western blot on human samples (fig 6e). Nucleic Acids Res (2017) ncbi
mouse monoclonal (G-3)
  • chromatin immunoprecipitation; rat; fig 5
Santa Cruz Biotechnology Pax5 antibody (SantaCruz, sc-377181) was used in chromatin immunoprecipitation on rat samples (fig 5). Mol Cell Endocrinol (2016) ncbi
mouse monoclonal (A-11)
  • western blot; chicken; fig 4
In order to investigate the requirement for Bcl6 for somatic hypermutation and gene conversion in chicken DT40 cells, Santa Cruz Biotechnology Pax5 antibody (Santa Cruz, sc-13146) was used in western blot on chicken samples (fig 4). PLoS ONE (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR3730(2))
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s3c
Abcam Pax5 antibody (Abcam, EPR3730(2)) was used in immunohistochemistry - paraffin section on mouse samples (fig s3c). Science (2018) ncbi
domestic rabbit monoclonal (EPR3730(2))
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig st12
In order to outline the protocols for antibodies used for immunohistochemical studies, Abcam Pax5 antibody (Abcam, ab109443) was used in immunohistochemistry - paraffin section on mouse samples at 1:300 (fig st12). J Toxicol Pathol (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 3e
  • western blot; human; loading ...; fig 6e
  • immunoprecipitation; mouse; loading ...; fig s8e
  • western blot; mouse; loading ...; fig s8e
In order to explore transcriptional regulation mediated by p27, Pax5, and PCAF, Abcam Pax5 antibody (Abcam, ab15164) was used in ChIP-Seq on human samples (fig 3e), in western blot on human samples (fig 6e), in immunoprecipitation on mouse samples (fig s8e) and in western blot on mouse samples (fig s8e). Nucleic Acids Res (2017) ncbi
BioLegend
rat monoclonal (1H9)
  • flow cytometry; human; loading ...; fig 2b
BioLegend Pax5 antibody (Biolegend, 1H9) was used in flow cytometry on human samples (fig 2b). Arthritis Res Ther (2019) ncbi
Invitrogen
domestic rabbit monoclonal (SP34)
  • immunohistochemistry; human; tbl 2
In order to study a northern New England hospital and increased utilization, verification, and clinical implications of immunocytochemistry, Invitrogen Pax5 antibody (Thermo Scientific, SP34) was used in immunohistochemistry on human samples (tbl 2). Diagn Cytopathol (2015) ncbi
domestic rabbit monoclonal (SP34)
  • immunohistochemistry - paraffin section; human; 1:50; fig s1
In order to develop an "antigen relaxing" method to improve routine detection of scarce antigens in formalin-fixed, paraffin-embedded material, Invitrogen Pax5 antibody (Thermo Scientific, SP34) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig s1). Appl Immunohistochem Mol Morphol (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D19F8)
  • western blot; human; fig 6f
Cell Signaling Technology Pax5 antibody (Cell Signaling, 8970S) was used in western blot on human samples (fig 6f). Cell (2018) ncbi
domestic rabbit monoclonal (D19F8)
  • western blot; human; fig 2b
  • western blot; mouse; fig s3d
Cell Signaling Technology Pax5 antibody (Cell Signaling, 8970) was used in western blot on human samples (fig 2b) and in western blot on mouse samples (fig s3d). Nature (2017) ncbi
domestic rabbit monoclonal (D19F8)
  • western blot; human; fig s10b
Cell Signaling Technology Pax5 antibody (Cell Signaling, D19F8) was used in western blot on human samples (fig s10b). Nature (2015) ncbi
BD Biosciences
mouse monoclonal (24/Pax-5)
  • immunohistochemistry - paraffin section; horses; loading ...; fig s1b
BD Biosciences Pax5 antibody (BD Transduction, 24/Pax-5) was used in immunohistochemistry - paraffin section on horses samples (fig s1b). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (24/Pax-5)
  • immunohistochemistry - paraffin section; human; 1:35; loading ...; fig 4b
In order to describe the evolution of ibrutinib resistance using samples from five patients with chronic lymphocytic leukemia, BD Biosciences Pax5 antibody (BD Biosciences, 24) was used in immunohistochemistry - paraffin section on human samples at 1:35 (fig 4b). Nat Commun (2016) ncbi
mouse monoclonal (24/Pax-5)
  • immunohistochemistry - paraffin section; dogs
BD Biosciences Pax5 antibody (BD Biosciences, 24/Pax-5) was used in immunohistochemistry - paraffin section on dogs samples . Vet Clin Pathol (2015) ncbi
mouse monoclonal (24/Pax-5)
  • immunohistochemistry - paraffin section; human
In order to investigate the clinical and pathological features in a girl with common variable immunodeficiency, BD Biosciences Pax5 antibody (BD Biosciences, 24) was used in immunohistochemistry - paraffin section on human samples . Int J Surg Pathol (2014) ncbi
Articles Reviewed
  1. Ye Y, Liu M, Tang L, Du F, Liu Y, Hao P, et al. Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis. Arthritis Res Ther. 2019;21:92 pubmed publisher
  2. Poffenberger M, Metcalfe Roach A, Aguilar E, Chen J, Hsu B, Wong A, et al. LKB1 deficiency in T cells promotes the development of gastrointestinal polyposis. Science. 2018;361:406-411 pubmed publisher
  3. Xiao G, Chan L, Klemm L, Braas D, Chen Z, Geng H, et al. B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies. Cell. 2018;173:470-484.e18 pubmed publisher
  4. Kurkewich J, Hansen J, Klopfenstein N, Zhang H, Wood C, Boucher A, et al. The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis. PLoS Genet. 2017;13:e1006887 pubmed publisher
  5. Pfaender S, Walter S, Grabski E, Todt D, Bruening J, Romero Brey I, et al. Immune protection against reinfection with nonprimate hepacivirus. Proc Natl Acad Sci U S A. 2017;114:E2430-E2439 pubmed publisher
  6. Chan L, Chen Z, Braas D, Lee J, Xiao G, Geng H, et al. Metabolic gatekeeper function of B-lymphoid transcription factors. Nature. 2017;542:479-483 pubmed publisher
  7. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  8. Perearnau A, Orlando S, Islam A, Gallastegui E, Martinez J, Jordan A, et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res. 2017;45:5086-5099 pubmed publisher
  9. Rossich L, Thomasz L, Nicola J, Nazar M, Salvarredi L, Pisarev M, et al. Effects of 2-iodohexadecanal in the physiology of thyroid cells. Mol Cell Endocrinol. 2016;437:292-301 pubmed publisher
  10. Burger J, Landau D, Taylor Weiner A, Bozic I, Zhang H, Sarosiek K, et al. Clonal evolution in patients with chronic lymphocytic leukaemia developing resistance to BTK inhibition. Nat Commun. 2016;7:11589 pubmed publisher
  11. Williams A, Maman Y, Alinikula J, Schatz D. Bcl6 Is Required for Somatic Hypermutation and Gene Conversion in Chicken DT40 Cells. PLoS ONE. 2016;11:e0149146 pubmed publisher
  12. Sauter J, Ambaye A, Mount S. Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital. Diagn Cytopathol. 2015;43:688-95 pubmed publisher
  13. Scalia C, Gendusa R, Cattoretti G. A 2-Step Laemmli and Antigen Retrieval Method Improves Immunodetection. Appl Immunohistochem Mol Morphol. 2016;24:436-46 pubmed publisher
  14. Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher
  15. Stokol T, Schaefer D, Shuman M, Belcher N, Dong L. Alkaline phosphatase is a useful cytochemical marker for the diagnosis of acute myelomonocytic and monocytic leukemia in the dog. Vet Clin Pathol. 2015;44:79-93 pubmed publisher
  16. Petrilli G, Lorenzi L, Paracchini R, Ubiali A, Schumacher R, Cabassa P, et al. Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature. Int J Surg Pathol. 2014;22:712-21 pubmed publisher