This is a Validated Antibody Database (VAD) review about mouse Panx1, based on 9 published articles (read how Labome selects the articles), using Panx1 antibody in all methods. It is aimed to help Labome visitors find the most suited Panx1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Panx1 synonym: AI847747; pannexin-1

Invitrogen
rabbit polyclonal
  • western blot; mouse; 1:100; fig 2
In order to study impairment of bone mechanosignaling under high glucose levels linked to type-1 diabetes through a P2X7R-Panx1 complex, Invitrogen Panx1 antibody (Invitrogen, 487900) was used in western blot on mouse samples at 1:100 (fig 2). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; fig 1
In order to study the spreading depolarization-induced dendritic beading where neuronal pannexin-1 channels are not molecular routes of water influx, Invitrogen Panx1 antibody (Invitrogen, 488100) was used in immunohistochemistry on mouse samples at 1:250 (fig 1). J Cereb Blood Flow Metab (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 1
In order to determine the role of Duox2 and how it regulates Pannexin 1-mediated ATP release in primary human airway epithelial cells via changes in intracellular pH and not H202 production, Invitrogen Panx1 antibody (Invitrogen, 488100) was used in western blot on human samples (fig 1). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
In order to identify the epigenetic mechanism involved in the increased Panx1 expression in the dorsal root ganglion after nerve injury, Invitrogen Panx1 antibody (Invitrogen, 488000) was used in western blot on rat samples at 1:1000. J Biol Chem (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:100
  • western blot; rat; 1:1000
In order to study pannexin-1 and it role in the activation of innate immune cells of the choroid plexus, Invitrogen Panx1 antibody (Invitrogen, 488100) was used in immunocytochemistry on rat samples at 1:100 and in western blot on rat samples at 1:1000. Channels (Austin) (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; fig 2
In order to identify mechanisms of Panx1 activation, Invitrogen Panx1 antibody (Invitrogen, 488100) was used in immunohistochemistry on mouse samples at 1:1000 (fig 2). J Neurosci (2012) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:500; fig 3
  • western blot; rat; 1:1000; fig 2
In order to discuss the role of NLRP1/NALP1 during central nervous system injury, Invitrogen Panx1 antibody (Invitrogen, 488100) was used in immunohistochemistry - frozen section on rat samples at 1:500 (fig 3) and in western blot on rat samples at 1:1000 (fig 2). BMC Neurosci (2011) ncbi
R&D Systems
mouse monoclonal (720505)
  • western blot; mouse; 1:500; fig 2
In order to study differential expression of astrocytic connexins by a prenatal alcohol exposure mouse model, R&D Systems Panx1 antibody (R&D Systems, MAB 7097) was used in western blot on mouse samples at 1:500 (fig 2). Neurobiol Dis (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:5000; loading ...; fig 6a
In order to show that serum- and glucocorticoid-inducible kinase-1 governs the glucocorticoid-enhanced release of ATP from astrocytes, Abcam Panx1 antibody (Abcam, ab124131) was used in western blot on mouse samples at 1:5000 (fig 6a). Nat Commun (2016) ncbi
Articles Reviewed
  1. Koyanagi S, Kusunose N, Taniguchi M, Akamine T, Kanado Y, Ozono Y, et al. Glucocorticoid regulation of ATP release from spinal astrocytes underlies diurnal exacerbation of neuropathic mechanical allodynia. Nat Commun. 2016;7:13102 pubmed publisher
  2. Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher
  3. Sword J, Croom D, Wang P, Thompson R, Kirov S. Neuronal pannexin-1 channels are not molecular routes of water influx during spreading depolarization-induced dendritic beading. J Cereb Blood Flow Metab. 2017;37:1626-1633 pubmed publisher
  4. Ramani M, Mylvaganam S, Krawczyk M, Wang L, Zoidl C, Brien J, et al. Differential expression of astrocytic connexins in a mouse model of prenatal alcohol exposure. Neurobiol Dis. 2016;91:83-93 pubmed publisher
  5. Krick S, Wang J, St Pierre M, Gonzalez C, Dahl G, Salathe M. Dual Oxidase 2 (Duox2) Regulates Pannexin 1-mediated ATP Release in Primary Human Airway Epithelial Cells via Changes in Intracellular pH and Not H2O2 Production. J Biol Chem. 2016;291:6423-32 pubmed publisher
  6. Zhang Y, Laumet G, Chen S, Hittelman W, Pan H. Pannexin-1 Up-regulation in the Dorsal Root Ganglion Contributes to Neuropathic Pain Development. J Biol Chem. 2015;290:14647-55 pubmed publisher
  7. Maslieieva V, Thompson R. A critical role for pannexin-1 in activation of innate immune cells of the choroid plexus. Channels (Austin). 2014;8:131-41 pubmed publisher
  8. Weilinger N, Tang P, Thompson R. Anoxia-induced NMDA receptor activation opens pannexin channels via Src family kinases. J Neurosci. 2012;32:12579-88 pubmed publisher
  9. Mawhinney L, de Rivero Vaccari J, Dale G, Keane R, Bramlett H. Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats. BMC Neurosci. 2011;12:123 pubmed publisher