This is a Validated Antibody Database (VAD) review about mouse Padi4, based on 13 published articles (read how Labome selects the articles), using Padi4 antibody in all methods. It is aimed to help Labome visitors find the most suited Padi4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Padi4 synonym: Pad4; Pdi4

Knockout validation
Abcam
domestic rabbit polyclonal
  • immunocytochemistry knockout validation; mouse; fig 5c
  • western blot knockout validation; mouse; fig 5b
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1d
Abcam Padi4 antibody (Abcam, ab96758) was used in immunocytochemistry knockout validation on mouse samples (fig 5c), in western blot knockout validation on mouse samples (fig 5b) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1d). Theranostics (2021) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; hamsters; 1:100; loading ...; fig 12a
  • immunohistochemistry - paraffin section; bovine; 1:100; loading ...; fig 8a, 14a
  • immunohistochemistry - paraffin section; domestic sheep; 1:100; fig 5a
  • immunohistochemistry - paraffin section; domestic rabbit; 1:100; loading ...; fig 2a, 3a
  • immunohistochemistry - paraffin section; dogs; 1:100; loading ...; fig 11a, 19a
  • immunohistochemistry - paraffin section; cat; 1:100; fig 13a, 16a
Abcam Padi4 antibody (Abcam, ab50247) was used in immunohistochemistry - paraffin section on hamsters samples at 1:100 (fig 12a), in immunohistochemistry - paraffin section on bovine samples at 1:100 (fig 8a, 14a), in immunohistochemistry - paraffin section on domestic sheep samples at 1:100 (fig 5a), in immunohistochemistry - paraffin section on domestic rabbit samples at 1:100 (fig 2a, 3a), in immunohistochemistry - paraffin section on dogs samples at 1:100 (fig 11a, 19a) and in immunohistochemistry - paraffin section on cat samples at 1:100 (fig 13a, 16a). Int J Mol Sci (2022) ncbi
domestic rabbit polyclonal
  • immunocytochemistry knockout validation; mouse; fig 5c
  • western blot knockout validation; mouse; fig 5b
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1d
Abcam Padi4 antibody (Abcam, ab96758) was used in immunocytochemistry knockout validation on mouse samples (fig 5c), in western blot knockout validation on mouse samples (fig 5b) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1d). Theranostics (2021) ncbi
mouse monoclonal (OTI4H5)
  • chromatin immunoprecipitation; human; loading ...; fig 3c, s4b
  • immunohistochemistry; human; loading ...; fig 6d
  • western blot; human; 1:500; loading ...; fig 1a
Abcam Padi4 antibody (Abcam, ab128086) was used in chromatin immunoprecipitation on human samples (fig 3c, s4b), in immunohistochemistry on human samples (fig 6d) and in western blot on human samples at 1:500 (fig 1a). Sci Adv (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Abcam Padi4 antibody (Abcam, ab50247) was used in western blot on human samples (fig 2a). Mol Cell (2019) ncbi
mouse monoclonal (OTI4H5)
  • western blot; human; 1:2000; fig s11
Abcam Padi4 antibody (Abcam, ab128086) was used in western blot on human samples at 1:2000 (fig s11). Nat Commun (2016) ncbi
mouse monoclonal (OTI4H5)
  • western blot; human; fig 3
In order to test if dying neutrophils release peptidyl arginine deiminase, which results in citrullination of antigens relevant to rheumatoid arthritis, Abcam Padi4 antibody (Abcam, ab128086) was used in western blot on human samples (fig 3). Arthritis Res Ther (2015) ncbi
mouse monoclonal (OTI4H5)
  • immunohistochemistry - frozen section; human; loading ...; fig 4
Abcam Padi4 antibody (Abcam, ab128086) was used in immunohistochemistry - frozen section on human samples (fig 4). Arthritis Rheumatol (2015) ncbi
mouse monoclonal (OTI4H5)
  • immunocytochemistry; human; 1:100
Abcam Padi4 antibody (Abcam, ab128086) was used in immunocytochemistry on human samples at 1:100. Respir Res (2015) ncbi
mouse monoclonal (OTI4H5)
  • immunocytochemistry; human; 1:100
Abcam Padi4 antibody (Abcam, ab128086) was used in immunocytochemistry on human samples at 1:100. PLoS ONE (2014) ncbi
mouse monoclonal (OTI4H5)
  • immunohistochemistry - paraffin section; human; fig 1c
Abcam Padi4 antibody (Abcam, ab128086) was used in immunohistochemistry - paraffin section on human samples (fig 1c). J Thromb Haemost (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 10 ug/ml; loading ...; fig 1f
In order to study the contribution of neutrophil extracellular traps to pulmonary hypertension, Invitrogen Padi4 antibody (ThermoScientific, PA5-22317) was used in immunohistochemistry - paraffin section on human samples at 10 ug/ml (fig 1f). Arterioscler Thromb Vasc Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; rhesus macaque; 1:10; fig 9
In order to examine the role of B cells in the common marmoset and rhesus monkey models of experimental autoimmune encephalomyelitis, Invitrogen Padi4 antibody (Invitrogen, PA5-22317) was used in immunocytochemistry on rhesus macaque samples at 1:10 (fig 9). J Immunol (2016) ncbi
BioLegend
mouse monoclonal (O94H5)
  • western blot; mouse; 1:1000; loading ...; fig 6b
BioLegend Padi4 antibody (Biolegend, O94H5) was used in western blot on mouse samples at 1:1000 (fig 6b). Nat Commun (2020) ncbi
Articles Reviewed
  1. Inal J, Hristova M, Lange S. A Pilot Study on Peptidylarginine Deiminases and Protein Deimination in Animal Cancers across Vertebrate Species. Int J Mol Sci. 2022;23: pubmed publisher
  2. Ni Y, Hu B, Wu G, Shao Z, Zheng Y, Zhang R, et al. Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI. Theranostics. 2021;11:9431-9451 pubmed publisher
  3. Wang Y, Lyu Y, Tu K, Xu Q, Yang Y, Salman S, et al. Histone citrullination by PADI4 is required for HIF-dependent transcriptional responses to hypoxia and tumor vascularization. Sci Adv. 2021;7: pubmed publisher
  4. Kang L, Yu H, Yang X, Zhu Y, Bai X, Wang R, et al. Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke. Nat Commun. 2020;11:2488 pubmed publisher
  5. Sharma P, Lioutas A, Fernandez Fuentes N, Quilez J, Carbonell Caballero J, Wright R, et al. Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription. Mol Cell. 2019;73:84-96.e7 pubmed publisher
  6. Aldabbous L, Abdul Salam V, McKinnon T, Duluc L, Pepke Zaba J, Southwood M, et al. Neutrophil Extracellular Traps Promote Angiogenesis: Evidence From Vascular Pathology in Pulmonary Hypertension. Arterioscler Thromb Vasc Biol. 2016;36:2078-87 pubmed publisher
  7. Jagessar S, Holtman I, Hofman S, Morandi E, Heijmans N, Laman J, et al. Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte Glycoprotein. J Immunol. 2016;197:1074-88 pubmed publisher
  8. Katkar G, Sundaram M, NaveenKumar S, Swethakumar B, Sharma R, Paul M, et al. NETosis and lack of DNase activity are key factors in Echis carinatus venom-induced tissue destruction. Nat Commun. 2016;7:11361 pubmed publisher
  9. Blachère N, Parveen S, Fak J, Frank M, Orange D. Inflammatory but not apoptotic death of granulocytes citrullinates fibrinogen. Arthritis Res Ther. 2015;17:369 pubmed publisher
  10. Spengler J, Lugonja B, Ytterberg A, Zubarev R, Creese A, Pearson M, et al. Release of Active Peptidyl Arginine Deiminases by Neutrophils Can Explain Production of Extracellular Citrullinated Autoantigens in Rheumatoid Arthritis Synovial Fluid. Arthritis Rheumatol. 2015;67:3135-45 pubmed publisher
  11. Grabcanovic Musija F, Obermayer A, Stoiber W, Krautgartner W, Steinbacher P, Winterberg N, et al. Neutrophil extracellular trap (NET) formation characterises stable and exacerbated COPD and correlates with airflow limitation. Respir Res. 2015;16:59 pubmed publisher
  12. Obermayer A, Stoiber W, Krautgartner W, Klappacher M, Kofler B, Steinbacher P, et al. New aspects on the structure of neutrophil extracellular traps from chronic obstructive pulmonary disease and in vitro generation. PLoS ONE. 2014;9:e97784 pubmed publisher
  13. Savchenko A, Martinod K, Seidman M, Wong S, Borissoff J, Piazza G, et al. Neutrophil extracellular traps form predominantly during the organizing stage of human venous thromboembolism development. J Thromb Haemost. 2014;12:860-70 pubmed publisher