This is a Validated Antibody Database (VAD) review about mouse PP2A, based on 11 published articles (read how Labome selects the articles), using PP2A antibody in all methods. It is aimed to help Labome visitors find the most suited PP2A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PP2A synonym: 6330556D22Rik; PP2A; PR65; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; protein phosphatase PP2A; serine/threonine protein phosphatase A subunit type 2A

Knockout validation
Cell Signaling Technology
rabbit monoclonal (81G5)
  • western blot knockout validation; human; fig 7a
  • western blot; mouse; fig 6e
Cell Signaling Technology PP2A antibody (Cell Signaling, 2041) was used in western blot knockout validation on human samples (fig 7a) and in western blot on mouse samples (fig 6e). Cell (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (4G7)
  • immunoprecipitation; rat; loading ...; fig 2a
  • immunocytochemistry; rat; 1:50; loading ...; fig 4a
  • western blot; rat; 1:500; loading ...; fig 2a
Santa Cruz Biotechnology PP2A antibody (Santa Cruz Biotechnology, 4G7) was used in immunoprecipitation on rat samples (fig 2a), in immunocytochemistry on rat samples at 1:50 (fig 4a) and in western blot on rat samples at 1:500 (fig 2a). J Neurochem (2016) ncbi
mouse monoclonal (4G7)
  • immunocytochemistry; rat; 1:200
  • western blot; rat; 1:6000
In order to examine the role of Set-beta in neurons, Santa Cruz Biotechnology PP2A antibody (SCBT, sc13600) was used in immunocytochemistry on rat samples at 1:200 and in western blot on rat samples at 1:6000. J Neurosci (2014) ncbi
Bethyl
rabbit polyclonal
  • immunohistochemistry - frozen section; human; fig 4a
  • flow cytometry; human; fig 2g
  • western blot; human; fig 2f
Bethyl PP2A antibody (Bethyl Laboratories, A300-963A) was used in immunohistochemistry - frozen section on human samples (fig 4a), in flow cytometry on human samples (fig 2g) and in western blot on human samples (fig 2f). Proc Natl Acad Sci U S A (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (81G5)
  • western blot knockout validation; human; fig 7a
  • western blot; mouse; fig 6e
Cell Signaling Technology PP2A antibody (Cell Signaling, 2041) was used in western blot knockout validation on human samples (fig 7a) and in western blot on mouse samples (fig 6e). Cell (2018) ncbi
rabbit monoclonal (81G5)
  • western blot; human; loading ...; fig s11e
Cell Signaling Technology PP2A antibody (Cell Signaling, 2041S) was used in western blot on human samples (fig s11e). J Clin Invest (2017) ncbi
rabbit polyclonal
  • western blot; mouse; fig s6
Cell Signaling Technology PP2A antibody (Cell Signaling, 2039) was used in western blot on mouse samples (fig s6). PLoS Genet (2016) ncbi
rabbit monoclonal (81G5)
  • western blot; human; loading ...; fig 1c
Cell Signaling Technology PP2A antibody (Cell Signaling Technology, 2041) was used in western blot on human samples (fig 1c). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to study triple-negative breast cancer and BET bromodomain inhibitor response and resistance, Cell Signaling Technology PP2A antibody (Cell Signaling, 2039) was used in western blot on human samples (fig 4). Nature (2016) ncbi
rabbit monoclonal (81G5)
  • western blot; human; 1:1000; fig 6
  • western blot; mouse; 1:1000; fig s3
Cell Signaling Technology PP2A antibody (Cell Signaling, 2041) was used in western blot on human samples at 1:1000 (fig 6) and in western blot on mouse samples at 1:1000 (fig s3). Nat Commun (2015) ncbi
rabbit monoclonal (81G5)
  • immunocytochemistry; mouse; 1:100; fig 2
  • western blot; mouse; 1:1000; fig 3
  • western blot; human; 1:1000; fig s7
Cell Signaling Technology PP2A antibody (Cell Signaling, 2041) was used in immunocytochemistry on mouse samples at 1:100 (fig 2), in western blot on mouse samples at 1:1000 (fig 3) and in western blot on human samples at 1:1000 (fig s7). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Cell Signaling Technology PP2A antibody (Cell Signaling Technology., 2039S) was used in western blot on human samples (fig 5). Cell Rep (2014) ncbi
Articles Reviewed
  1. Xiao G, Chan L, Klemm L, Braas D, Chen Z, Geng H, et al. B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies. Cell. 2018;173:470-484.e18 pubmed publisher
  2. Sangodkar J, Perl A, Tohme R, Kiselar J, Kastrinsky D, Zaware N, et al. Activation of tumor suppressor protein PP2A inhibits KRAS-driven tumor growth. J Clin Invest. 2017;127:2081-2090 pubmed publisher
  3. Diril M, Bisteau X, Kitagawa M, Caldez M, Wee S, Gunaratne J, et al. Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet. 2016;12:e1006310 pubmed publisher
  4. Juraszek B, Nałęcz K. Protein phosphatase PP2A - a novel interacting partner of carnitine transporter OCTN2 (SLC22A5) in rat astrocytes. J Neurochem. 2016;139:537-551 pubmed publisher
  5. Liang J, Cao R, Zhang Y, Xia Y, Zheng Y, Li X, et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun. 2016;7:12431 pubmed publisher
  6. Shu S, Lin C, He H, Witwicki R, Tabassum D, Roberts J, et al. Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer. Nature. 2016;529:413-417 pubmed publisher
  7. Wong P, Feng Y, Wang J, Shi R, Jiang X. Regulation of autophagy by coordinated action of mTORC1 and protein phosphatase 2A. Nat Commun. 2015;6:8048 pubmed publisher
  8. Yu C, Ji S, Sha Q, Sun Q, Fan H. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun. 2015;6:8017 pubmed publisher
  9. Christianson D, Dobroff A, Proneth B, Zurita A, Salameh A, Dondossola E, et al. Ligand-directed targeting of lymphatic vessels uncovers mechanistic insights in melanoma metastasis. Proc Natl Acad Sci U S A. 2015;112:2521-6 pubmed publisher
  10. Trakhtenberg E, Wang Y, Morkin M, Fernandez S, Mlacker G, Shechter J, et al. Regulating Set-?'s Subcellular Localization Toggles Its Function between Inhibiting and Promoting Axon Growth and Regeneration. J Neurosci. 2014;34:7361-74 pubmed publisher
  11. Wang J, Xu X, Alontaga A, Chen Y, Liu Y. Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction. Cell Rep. 2014;7:848-58 pubmed publisher