This is a Validated Antibody Database (VAD) review about mouse P2ry12, based on 16 published articles (read how Labome selects the articles), using P2ry12 antibody in all methods. It is aimed to help Labome visitors find the most suited P2ry12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
P2ry12 synonym: 2900079B22Rik; 4921504D23Rik; P2Y12

BioLegend
rat monoclonal (S16007D)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 2f
BioLegend P2ry12 antibody (BioLegend, 848002) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2f). Proc Natl Acad Sci U S A (2022) ncbi
rat monoclonal (S16007D)
  • flow cytometry; mouse; 1:200; loading ...; fig 3e
BioLegend P2ry12 antibody (Biolegend, 848006) was used in flow cytometry on mouse samples at 1:200 (fig 3e). Aging Cell (2021) ncbi
rat monoclonal (S16007D)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5b
BioLegend P2ry12 antibody (Biolegend, 848002) was used in immunohistochemistry - frozen section on mouse samples (fig 5b). Neuron (2021) ncbi
rat monoclonal (S16007D)
  • flow cytometry; mouse; 1:100; loading ...; fig 5g
BioLegend P2ry12 antibody (Biolegend, S16007D) was used in flow cytometry on mouse samples at 1:100 (fig 5g). Nat Neurosci (2021) ncbi
rat monoclonal (S16007D)
  • flow cytometry; mouse; 1:200; loading ...; fig 1d
BioLegend P2ry12 antibody (BioLegend, S16007D) was used in flow cytometry on mouse samples at 1:200 (fig 1d). elife (2020) ncbi
rat monoclonal (S16007D)
  • flow cytometry; mouse; loading ...; fig 6c
BioLegend P2ry12 antibody (Biolegend, S16007D) was used in flow cytometry on mouse samples (fig 6c). Front Immunol (2019) ncbi
Abcam
domestic rabbit monoclonal (EPR18611)
  • western blot; human; loading ...; fig 1a
Abcam P2ry12 antibody (Abcam, EPR18611) was used in western blot on human samples (fig 1a). Cancers (Basel) (2020) ncbi
domestic rabbit monoclonal (EPR18611)
  • immunohistochemistry - frozen section; rat; 1:150; loading ...; fig 2
Abcam P2ry12 antibody (Abcam, ab184411) was used in immunohistochemistry - frozen section on rat samples at 1:150 (fig 2). Cell Physiol Biochem (2018) ncbi
domestic rabbit monoclonal (EPR18611)
  • western blot; human; loading ...; fig s4
Abcam P2ry12 antibody (Abcam, EPR18611) was used in western blot on human samples (fig s4). Cancers (Basel) (2017) ncbi
domestic rabbit monoclonal (EPR18611)
  • western blot; rat; 1:1000; fig 2c
Abcam P2ry12 antibody (Abcam, ab184411) was used in western blot on rat samples at 1:1000 (fig 2c). J Neuroinflammation (2017) ncbi
Invitrogen
domestic rabbit recombinant (4H5L19)
  • western blot; mouse; 1:1000; loading ...; fig s5b
Invitrogen P2ry12 antibody (Thermo Fisher, 702516) was used in western blot on mouse samples at 1:1000 (fig s5b). Nat Commun (2020) ncbi
domestic rabbit recombinant (4H5L19)
  • western blot; human; loading ...; fig s1a
Invitrogen P2ry12 antibody (ThermoFischer, 4H5L19) was used in western blot on human samples (fig s1a). Cancers (Basel) (2020) ncbi
Alomone Labs
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 2j
Alomone Labs P2ry12 antibody (Alomone Labs, APR-012) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 2j). Front Immunol (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1
Alomone Labs P2ry12 antibody (Alomone Labs, APR-012) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1). Int J Mol Sci (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 1b
Alomone Labs P2ry12 antibody (Alomone Labs, APR-020) was used in immunocytochemistry on human samples at 1:100 (fig 1b). Mol Psychiatry (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; tbl 1
In order to study P2Y12 expression in human brain tissue, Alomone Labs P2ry12 antibody (Alomone, APR-020) was used in immunohistochemistry - paraffin section on human samples (tbl 1). Glia (2017) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:100; fig 4a
In order to test if the microglial P2Y12 receptor in the trigeminal spinal subnucleus caudalis is involved in intraoral cancer pain, MilliporeSigma P2ry12 antibody (Sigma, P4871) was used in immunohistochemistry - free floating section on rat samples at 1:100 (fig 4a). J Dent Res (2016) ncbi
Articles Reviewed
  1. Zhao Q, Dai W, Chen H, Jacobs R, Zlokovic B, Lund B, et al. Prenatal disruption of blood-brain barrier formation via cyclooxygenase activation leads to lifelong brain inflammation. Proc Natl Acad Sci U S A. 2022;119:e2113310119 pubmed publisher
  2. Funk K, Arutyunov A, Desai P, White J, Soung A, Rosen S, et al. Decreased antiviral immune response within the central nervous system of aged mice is associated with increased lethality of West Nile virus encephalitis. Aging Cell. 2021;20:e13412 pubmed publisher
  3. Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed publisher
  4. Safaiyan S, Besson Girard S, Kaya T, Cantuti Castelvetri L, Liu L, Ji H, et al. White matter aging drives microglial diversity. Neuron. 2021;109:1100-1117.e10 pubmed publisher
  5. Mastorakos P, Mihelson N, Luby M, Burks S, Johnson K, Hsia A, et al. Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury. Nat Neurosci. 2021;24:245-258 pubmed publisher
  6. BURNS J, Cotleur B, Walther D, Bajrami B, Rubino S, Wei R, et al. Differential accumulation of storage bodies with aging defines discrete subsets of microglia in the healthy brain. elife. 2020;9: pubmed publisher
  7. Xu Z, Kim G, Tan J, Riso A, Sun Y, Xu E, et al. Elevated protein synthesis in microglia causes autism-like synaptic and behavioral aberrations. Nat Commun. 2020;11:1797 pubmed publisher
  8. Bell O, Copland D, Ward A, Nicholson L, Lange C, Chu C, et al. Single Eye mRNA-Seq Reveals Normalisation of the Retinal Microglial Transcriptome Following Acute Inflammation. Front Immunol. 2019;10:3033 pubmed publisher
  9. Walker D, Tang T, Mendsaikhan A, Tooyama I, Serrano G, Sue L, et al. Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer's Disease Brains. Int J Mol Sci. 2020;21: pubmed publisher
  10. Elaskalani O, Domenichini A, Abdol Razak N, E Dye D, Falasca M, Metharom P. Antiplatelet Drug Ticagrelor Enhances Chemotherapeutic Efficacy by Targeting the Novel P2Y12-AKT Pathway in Pancreatic Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
  11. Sheng X, Wang J, Guo J, Xu Y, Jiang H, Zheng C, et al. Effects of Baicalin on Diabetic Cardiac Autonomic Neuropathy Mediated by the P2Y12 Receptor in Rat Stellate Ganglia. Cell Physiol Biochem. 2018;46:986-998 pubmed publisher
  12. Elaskalani O, Falasca M, Moran N, Berndt M, Metharom P. The Role of Platelet-Derived ADP and ATP in Promoting Pancreatic Cancer Cell Survival and Gemcitabine Resistance. Cancers (Basel). 2017;9: pubmed publisher
  13. Niu J, Huang D, Zhou R, Yue M, Xu T, Yang J, et al. Activation of dorsal horn cannabinoid CB2 receptor suppresses the expression of P2Y12 and P2Y13 receptors in neuropathic pain rats. J Neuroinflammation. 2017;14:185 pubmed publisher
  14. Sellgren C, Sheridan S, Gracias J, Xuan D, Fu T, Perlis R. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry. 2017;22:170-177 pubmed publisher
  15. Mildner A, Huang H, Radke J, Stenzel W, Priller J. P2Y12 receptor is expressed on human microglia under physiological conditions throughout development and is sensitive to neuroinflammatory diseases. Glia. 2017;65:375-387 pubmed publisher
  16. Tamagawa T, Shinoda M, Honda K, Furukawa A, Kaji K, Nagashima H, et al. Involvement of Microglial P2Y12 Signaling in Tongue Cancer Pain. J Dent Res. 2016;95:1176-82 pubmed publisher