This is a Validated Antibody Database (VAD) review about mouse P2rx7, based on 13 published articles (read how Labome selects the articles), using P2rx7 antibody in all methods. It is aimed to help Labome visitors find the most suited P2rx7 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
P2rx7 synonym: AI467586; P2X(7); P2X7R

Knockout validation
Alomone Labs
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s2b
In order to characterize the P2X7R-secretome in M1 and M2 macrophages., Alomone Labs P2rx7 antibody (Alomone, APR-004) was used in western blot knockout validation on mouse samples (fig s2b). Sci Rep (2016) ncbi
Alomone Labs
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; fig 3a
Alomone Labs P2rx7 antibody (Alomone, APR-008) was used in immunocytochemistry on mouse samples at 1:200 (fig 3a). J Neuroinflammation (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 6i
Alomone Labs P2rx7 antibody (Alomone labs, APR-004) was used in western blot on mouse samples (fig 6i). Sci Adv (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...
Alomone Labs P2rx7 antibody (Alomone Labs, APR-004) was used in immunohistochemistry on mouse samples . Anal Cell Pathol (Amst) (2021) ncbi
domestic rabbit polyclonal
  • proximity ligation assay; mouse; loading ...; fig 4a
Alomone Labs P2rx7 antibody (Alomone Labs, APR-008) was used in proximity ligation assay on mouse samples (fig 4a). Anal Cell Pathol (Amst) (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; loading ...; fig 6c
Alomone Labs P2rx7 antibody (Alomone, APR-008) was used in immunohistochemistry on mouse samples at 1:500 (fig 6c). Sci Rep (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5a
  • western blot; mouse; 1:200; loading ...; fig 5a
Alomone Labs P2rx7 antibody (Alomone, APR-004) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 5a) and in western blot on mouse samples at 1:200 (fig 5a). Chin Med J (Engl) (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 3a
In order to elucidate the role of P2X7 in alveolar epithelial cells, Alomone Labs P2rx7 antibody (alomone labs, APR-004) was used in western blot on mouse samples at 1:500 (fig 3a). Histochem Cell Biol (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 1
Alomone Labs P2rx7 antibody (Alomone labs, APR-008) was used in immunohistochemistry on mouse samples (fig 1). Neural Regen Res (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; fig 4b
In order to examine the contribution of the ATP-gated receptor P2X7 to pancreatic ductal adenocarcinoma, Alomone Labs P2rx7 antibody (Alomone, APR-008) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 4b). Int J Cancer (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; fig 4c
  • western blot; human; 1:500; fig 2f
In order to examine the contribution of the ATP-gated receptor P2X7 to pancreatic ductal adenocarcinoma, Alomone Labs P2rx7 antibody (Alomone, APR-004) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 4c) and in western blot on human samples at 1:500 (fig 2f). Int J Cancer (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s2b
In order to characterize the P2X7R-secretome in M1 and M2 macrophages., Alomone Labs P2rx7 antibody (Alomone, APR-004) was used in western blot knockout validation on mouse samples (fig s2b). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • flow cytometry; mouse; fig 2
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 2
Alomone Labs P2rx7 antibody (Alomone Labs, APR 008) was used in flow cytometry on mouse samples (fig 2), in immunocytochemistry on mouse samples (fig 2) and in western blot on mouse samples (fig 2). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 2
Alomone Labs P2rx7 antibody (Alomone Labs, APR 004) was used in western blot on mouse samples (fig 2). Nat Commun (2016) ncbi
BioLegend
rat monoclonal (1F11)
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 4a
BioLegend P2rx7 antibody (BioLegend, 148707) was used in flow cytometry on mouse samples at 5 ug/ml (fig 4a). Sci Transl Med (2022) ncbi
rat monoclonal (1F11)
  • flow cytometry; mouse; loading ...; fig s10a
BioLegend P2rx7 antibody (BioLegend, 148708) was used in flow cytometry on mouse samples (fig s10a). Nat Commun (2020) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:900; fig 4d
In order to examine the contribution of the ATP-gated receptor P2X7 to pancreatic ductal adenocarcinoma, Invitrogen P2rx7 antibody (Life Technologies, PA5-28020) was used in immunohistochemistry - paraffin section on human samples at 1:900 (fig 4d). Int J Cancer (2016) ncbi
Bio-Rad
rat monoclonal (Hano43)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1e
  • flow cytometry; mouse; loading ...; fig 1d
In order to elucidate how P2X7 regulates the contraction of intestinal CD4 positive effector T cells, Bio-Rad P2rx7 antibody (AbD Serotec, Hano43) was used in immunohistochemistry - frozen section on mouse samples (fig 1e) and in flow cytometry on mouse samples (fig 1d). Mucosal Immunol (2017) ncbi
Articles Reviewed
  1. Wennerberg E, Mukherjee S, Spada S, Hung C, Agrusa C, Chen C, et al. Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancer. Sci Transl Med. 2022;14:eabe8195 pubmed publisher
  2. Campagno K, Lu W, Jassim A, Albalawi F, Cenaj A, Tso H, et al. Rapid morphologic changes to microglial cells and upregulation of mixed microglial activation state markers induced by P2X7 receptor stimulation and increased intraocular pressure. J Neuroinflammation. 2021;18:217 pubmed publisher
  3. Cheng J, Dong Y, Ma J, Pan R, Liao Y, Kong X, et al. Microglial Calhm2 regulates neuroinflammation and contributes to Alzheimer's disease pathology. Sci Adv. 2021;7: pubmed publisher
  4. Rhodes G, Segars K, Lee Y, Hutcheon A, Rich C, Trinkaus Randall V. Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium. Anal Cell Pathol (Amst). 2021;2021:4793338 pubmed publisher
  5. Wang A, Wong V, Lee P, Bui B, Dudczig S, Vessey K, et al. Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout. Sci Rep. 2021;11:4184 pubmed publisher
  6. von Roemeling C, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, et al. Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity. Nat Commun. 2020;11:1508 pubmed publisher
  7. Hou X, Dong H, Sun L, Yang M, Cheng H, Chen Y. Purinergic 2X7 Receptor is Involved in the Podocyte Damage of Obesity-Related Glomerulopathy via Activating Nucleotide-Binding and Oligomerization Domain-Like Receptor Protein 3 Inflammasome. Chin Med J (Engl). 2018;131:2713-2725 pubmed publisher
  8. Hashimoto Hill S, Friesen L, Kim M, Kim C. Contraction of intestinal effector T cells by retinoic acid-induced purinergic receptor P2X7. Mucosal Immunol. 2017;10:912-923 pubmed publisher
  9. Barth K, Blasche R, Neiser A, Bramke S, Frank J, Kasper M. P2X7R-dependent regulation of glycogen synthase kinase 3β and claudin-18 in alveolar epithelial type I cells of mice lung. Histochem Cell Biol. 2016;146:757-768 pubmed publisher
  10. Jager S, Vaegter C. Avoiding experimental bias by systematic antibody validation. Neural Regen Res. 2016;11:1079-80 pubmed publisher
  11. Giannuzzo A, Saccomano M, Napp J, Ellegaard M, Alves F, Novak I. Targeting of the P2X7 receptor in pancreatic cancer and stellate cells. Int J Cancer. 2016;139:2540-52 pubmed publisher
  12. de Torre Minguela C, Barberà Cremades M, Gómez A, Martín Sánchez F, Pelegrín P. Macrophage activation and polarization modify P2X7 receptor secretome influencing the inflammatory process. Sci Rep. 2016;6:22586 pubmed publisher
  13. Karmakar M, Katsnelson M, Dubyak G, Pearlman E. Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1β secretion in response to ATP. Nat Commun. 2016;7:10555 pubmed publisher