This is a Validated Antibody Database (VAD) review about mouse Optn, based on 10 published articles (read how Labome selects the articles), using Optn antibody in all methods. It is aimed to help Labome visitors find the most suited Optn antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Optn synonym: 4930441O07Rik; FIP2; HYPL; NRP

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (C-2)
  • immunocytochemistry knockout validation; human; loading ...; fig 3c
  • western blot knockout validation; human; loading ...; fig 2d
  • immunohistochemistry; human; 1:100; loading ...; fig 1a
Santa Cruz Biotechnology Optn antibody (Santa Cruz Biotechnology, sc-166576) was used in immunocytochemistry knockout validation on human samples (fig 3c), in western blot knockout validation on human samples (fig 2d) and in immunohistochemistry on human samples at 1:100 (fig 1a). Cancer Med (2021) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; 1:5000; fig 5
  • western blot knockout validation; mouse; 1:5000; fig 5
Abcam Optn antibody (Abcam, ab23666) was used in western blot on human samples at 1:5000 (fig 5) and in western blot knockout validation on mouse samples at 1:5000 (fig 5). BMC Biol (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-2)
  • immunocytochemistry knockout validation; human; loading ...; fig 3c
  • western blot knockout validation; human; loading ...; fig 2d
  • immunohistochemistry; human; 1:100; loading ...; fig 1a
Santa Cruz Biotechnology Optn antibody (Santa Cruz Biotechnology, sc-166576) was used in immunocytochemistry knockout validation on human samples (fig 3c), in western blot knockout validation on human samples (fig 2d) and in immunohistochemistry on human samples at 1:100 (fig 1a). Cancer Med (2021) ncbi
mouse monoclonal (C-2)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 8e
Santa Cruz Biotechnology Optn antibody (Santa Cruz, sc-166576) was used in immunohistochemistry on mouse samples at 1:200 (fig 8e). Cell Death Dis (2018) ncbi
mouse monoclonal (C-2)
  • western blot; human; loading ...; fig 6a
Santa Cruz Biotechnology Optn antibody (Santa Cruz, sc-166576) was used in western blot on human samples (fig 6a). Cell Death Differ (2018) ncbi
mouse monoclonal (C-2)
  • western blot; human; 1:1000; fig s15
Santa Cruz Biotechnology Optn antibody (Santa Cruz, sc-166576) was used in western blot on human samples at 1:1000 (fig s15). Nat Commun (2016) ncbi
mouse monoclonal (C-2)
  • immunoprecipitation; human; fig s7
  • immunocytochemistry; human; 1:500; fig s5
  • western blot; human; 1:2000; fig 5
  • western blot; mouse; 1:2000; fig 5
Santa Cruz Biotechnology Optn antibody (Santa Cruz, sc-166576) was used in immunoprecipitation on human samples (fig s7), in immunocytochemistry on human samples at 1:500 (fig s5), in western blot on human samples at 1:2000 (fig 5) and in western blot on mouse samples at 1:2000 (fig 5). BMC Biol (2016) ncbi
mouse monoclonal (C-2)
  • western blot; mouse
Santa Cruz Biotechnology Optn antibody (Santa Cruz Biotechnology, sc-166576) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:300; loading ...; fig 9a
Abcam Optn antibody (Abcam, ab23666) was used in western blot on mouse samples at 1:300 (fig 9a). Oxid Med Cell Longev (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:1000; loading ...; fig ar1a
Abcam Optn antibody (Abcam, ab23666) was used in immunocytochemistry on mouse samples at 1:1000 (fig ar1a). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1d
Abcam Optn antibody (Abcam, ab23666) was used in western blot on mouse samples at 1:1000 (fig 1d). J Biol Chem (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:5000; fig 5
  • western blot knockout validation; mouse; 1:5000; fig 5
Abcam Optn antibody (Abcam, ab23666) was used in western blot on human samples at 1:5000 (fig 5) and in western blot knockout validation on mouse samples at 1:5000 (fig 5). BMC Biol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; tbl 4
In order to elucidate the function of the alpha2-linker of myosin VI, Abcam Optn antibody (AbCam, ab-23666) was used in western blot on human samples at 1:1000 (tbl 4). Nat Struct Mol Biol (2016) ncbi
Articles Reviewed
  1. Cheng Y, Liu M, Tang H, Chen B, Yang G, Zhao W, et al. iTRAQ-Based Quantitative Proteomics Indicated Nrf2/OPTN-Mediated Mitophagy Inhibits NLRP3 Inflammasome Activation after Intracerebral Hemorrhage. Oxid Med Cell Longev. 2021;2021:6630281 pubmed publisher
  2. Inokuchi S, Yoshizumi T, Toshima T, Itoh S, Yugawa K, Harada N, et al. Suppression of optineurin impairs the progression of hepatocellular carcinoma through regulating mitophagy. Cancer Med. 2021;10:1501-1514 pubmed publisher
  3. Evans C, Holzbaur E. Degradation of engulfed mitochondria is rate-limiting in Optineurin-mediated mitophagy in neurons. elife. 2020;9: pubmed publisher
  4. Chen K, Dai H, Yuan J, Chen J, Lin L, Zhang W, et al. Optineurin-mediated mitophagy protects renal tubular epithelial cells against accelerated senescence in diabetic nephropathy. Cell Death Dis. 2018;9:105 pubmed publisher
  5. Goiran T, Duplan E, Rouland L, El Manaa W, Lauritzen I, Dunys J, et al. Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation. Cell Death Differ. 2018;25:873-884 pubmed publisher
  6. Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
  7. Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  8. Pourcelot M, Zemirli N, Silva da Costa L, Loyant R, Garcin D, Vitour D, et al. The Golgi apparatus acts as a platform for TBK1 activation after viral RNA sensing. BMC Biol. 2016;14:69 pubmed publisher
  9. Wollscheid H, Biancospino M, He F, Magistrati E, Molteni E, Lupia M, et al. Diverse functions of myosin VI elucidated by an isoform-specific α-helix domain. Nat Struct Mol Biol. 2016;23:300-308 pubmed publisher
  10. Sharma A, Huard C, Vernochet C, Ziemek D, Knowlton K, Tyminski E, et al. Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6. PLoS ONE. 2014;9:e92608 pubmed publisher