This is a Validated Antibody Database (VAD) review about mouse Npc1, based on 7 published articles (read how Labome selects the articles), using Npc1 antibody in all methods. It is aimed to help Labome visitors find the most suited Npc1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Npc1 synonym: A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; spm

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5209)
  • western blot knockout validation; human; 1:2000; loading ...; fig 8c
Abcam Npc1 antibody (Abcam, ab134113) was used in western blot knockout validation on human samples at 1:2000 (fig 8c). elife (2019) ncbi
Abcam
domestic rabbit monoclonal (EPR5209)
  • western blot; mouse; 1:3000; loading ...; fig 1i
Abcam Npc1 antibody (Abcam, ab134113) was used in western blot on mouse samples at 1:3000 (fig 1i). Nat Commun (2021) ncbi
domestic rabbit monoclonal (EPR5209)
  • immunocytochemistry; human; loading ...; fig 1g
Abcam Npc1 antibody (Abcam, ab134113) was used in immunocytochemistry on human samples (fig 1g). EMBO J (2021) ncbi
domestic rabbit monoclonal (EPR5209)
  • western blot; human; 1:3000; loading ...
Abcam Npc1 antibody (Abcam, EPR5209) was used in western blot on human samples at 1:3000. Nature (2020) ncbi
domestic rabbit monoclonal (EPR5209)
  • western blot; mouse; loading ...; fig 1c
Abcam Npc1 antibody (Abcam, ab134113) was used in western blot on mouse samples (fig 1c). Dis Model Mech (2020) ncbi
domestic rabbit monoclonal (EPR5209)
  • western blot knockout validation; human; 1:2000; loading ...; fig 8c
Abcam Npc1 antibody (Abcam, ab134113) was used in western blot knockout validation on human samples at 1:2000 (fig 8c). elife (2019) ncbi
domestic rabbit monoclonal (EPR5209)
  • western blot; mouse; loading ...; fig 5a
Abcam Npc1 antibody (Abcam, ab134113) was used in western blot on mouse samples (fig 5a). Sci Rep (2017) ncbi
Novus Biologicals
domestic rabbit polyclonal (AP-MAB0708)
  • western blot; rat; 1:2500; loading ...; fig 4d
Novus Biologicals Npc1 antibody (Novus Biologicals, NB400-148) was used in western blot on rat samples at 1:2500 (fig 4d). Life Sci Alliance (2021) ncbi
Articles Reviewed
  1. Alesi N, Akl E, Khabibullin D, Liu H, Nidhiry A, Garner E, et al. TSC2 regulates lysosome biogenesis via a non-canonical RAGC and TFEB-dependent mechanism. Nat Commun. 2021;12:4245 pubmed publisher
  2. Guix F, Capitán A, Casadomé Perales Á, Palomares Perez I, López Del Castillo I, Miguel V, et al. Increased exosome secretion in neurons aging in vitro by NPC1-mediated endosomal cholesterol buildup. Life Sci Alliance. 2021;4: pubmed publisher
  3. Takahashi K, Kanerva K, Vanharanta L, Almeida Souza L, Lietha D, Olkkonen V, et al. ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange. EMBO J. 2021;40:e106871 pubmed publisher
  4. Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
  5. Rodriguez Gil J, Watkins Chow D, Baxter L, Elliot G, Harper U, Wincovitch S, et al. Genetic background modifies phenotypic severity and longevity in a mouse model of Niemann-Pick disease type C1. Dis Model Mech. 2020;13: pubmed publisher
  6. McCauliff L, Langan A, Li R, Ilnytska O, Bose D, Waghalter M, et al. Intracellular cholesterol trafficking is dependent upon NPC2 interaction with lysobisphosphatidic acid. elife. 2019;8: pubmed publisher
  7. Vienken H, Mabrouki N, Grabau K, Claas R, Rudowski A, Schömel N, et al. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype with enhanced lysosomal Ca2+ storage. Sci Rep. 2017;7:43575 pubmed publisher