This is a Validated Antibody Database (VAD) review about mouse Nix, based on 11 published articles (read how Labome selects the articles), using Nix antibody in all methods. It is aimed to help Labome visitors find the most suited Nix antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Nix synonym: C86132; D14Ertd719e; Nip3L; Nix

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (H-8)
  • western blot knockout validation; human; 1:100; loading ...; fig 4d
Santa Cruz Biotechnology Nix antibody (Santa Cruz, sc-166332) was used in western blot knockout validation on human samples at 1:100 (fig 4d). Cell Rep (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR4033)
  • western blot knockout validation; human; 1:1000; loading ...; fig 4d
Abcam Nix antibody (Abcam, Ab109414) was used in western blot knockout validation on human samples at 1:1000 (fig 4d). Cell Rep (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-8)
  • western blot knockout validation; human; 1:100; loading ...; fig 4d
Santa Cruz Biotechnology Nix antibody (Santa Cruz, sc-166332) was used in western blot knockout validation on human samples at 1:100 (fig 4d). Cell Rep (2021) ncbi
mouse monoclonal (H-8)
  • proximity ligation assay; human; 1:100; fig 1
  • immunocytochemistry; human; 1:100; fig 1
Santa Cruz Biotechnology Nix antibody (Santa Cruz, sc-166332) was used in proximity ligation assay on human samples at 1:100 (fig 1) and in immunocytochemistry on human samples at 1:100 (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (H-8)
  • immunoprecipitation; rat
  • western blot; rat; 1:1000
Santa Cruz Biotechnology Nix antibody (Santa Cruz Biotechnology, sc-166332) was used in immunoprecipitation on rat samples and in western blot on rat samples at 1:1000. CNS Neurosci Ther (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR4033)
  • western blot knockout validation; human; 1:1000; loading ...; fig 4d
Abcam Nix antibody (Abcam, Ab109414) was used in western blot knockout validation on human samples at 1:1000 (fig 4d). Cell Rep (2021) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; 1:1500; loading ...; fig 5c
Invitrogen Nix antibody (Thermo Fisher, PA5-29385) was used in western blot on mouse samples at 1:1500 (fig 5c). JCI Insight (2021) ncbi
Novus Biologicals
domestic rabbit polyclonal (545818)
  • western blot; human; 1:3000; loading ...; fig 6a
Novus Biologicals Nix antibody (Novus Biologicals, NBP1-88558) was used in western blot on human samples at 1:3000 (fig 6a). Nat Commun (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4R4B)
  • western blot; mouse; 1:1000; loading ...; fig 6a
Cell Signaling Technology Nix antibody (CST, 12396S) was used in western blot on mouse samples at 1:1000 (fig 6a). Antioxidants (Basel) (2020) ncbi
domestic rabbit monoclonal (D4R4B)
  • western blot; mouse; 1:1000; loading ...; fig 8e
Cell Signaling Technology Nix antibody (Cell Signaling, 12396) was used in western blot on mouse samples at 1:1000 (fig 8e). J Neurosci (2019) ncbi
domestic rabbit monoclonal (D4R4B)
  • western blot; human; 1:1000; loading ...; fig 6c
Cell Signaling Technology Nix antibody (Cell Signaling Technology, 12396) was used in western blot on human samples at 1:1000 (fig 6c). Sci Rep (2019) ncbi
domestic rabbit monoclonal (D4R4B)
  • western blot; human; 1:500; loading ...; fig 6a
Cell Signaling Technology Nix antibody (Cell Signaling, 12396) was used in western blot on human samples at 1:500 (fig 6a). Autophagy (2016) ncbi
domestic rabbit monoclonal (D4R4B)
  • western blot; human
Cell Signaling Technology Nix antibody (Cell Signaling, 12396) was used in western blot on human samples . Genes Dev (2016) ncbi
domestic rabbit monoclonal (D4R4B)
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse; 1:1000
Cell Signaling Technology Nix antibody (Cell Signaling Technology, 12396) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. Eur J Pharmacol (2015) ncbi
Articles Reviewed
  1. Xu L, Humphries F, Delagic N, Wang B, Holland A, Edgar K, et al. ECSIT is a critical limiting factor for cardiac function. JCI Insight. 2021;6: pubmed publisher
  2. Simpson C, Tokito M, Uppala R, Sarkar M, Gudjonsson J, Holzbaur E. NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell Rep. 2021;34:108689 pubmed publisher
  3. Choi G, Lee H, Chae C, Cho J, Jung Y, Kim J, et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun. 2021;12:487 pubmed publisher
  4. Meza Torres C, Hernández Camacho J, Cortés Rodríguez A, Fang L, Bui Thanh T, Rodríguez Bies E, et al. Resveratrol Regulates the Expression of Genes Involved in CoQ Synthesis in Liver in Mice Fed with High Fat Diet. Antioxidants (Basel). 2020;9: pubmed publisher
  5. Hass D, Barnstable C. Mitochondrial Uncoupling Protein 2 Knock-out Promotes Mitophagy to Decrease Retinal Ganglion Cell Death in a Mouse Model of Glaucoma. J Neurosci. 2019;39:3582-3596 pubmed publisher
  6. Okuyama K, Kitajima Y, Egawa N, Kitagawa H, Ito K, Aishima S, et al. Mieap-induced accumulation of lysosomes within mitochondria (MALM) regulates gastric cancer cell invasion under hypoxia by suppressing reactive oxygen species accumulation. Sci Rep. 2019;9:2822 pubmed publisher
  7. Choi H, Merceron C, Mangiavini L, Seifert E, Schipani E, Shapiro I, et al. Hypoxia promotes noncanonical autophagy in nucleus pulposus cells independent of MTOR and HIF1A signaling. Autophagy. 2016;12:1631-46 pubmed publisher
  8. O Neill K, Huang K, Zhang J, Chen Y, Luo X. Inactivation of prosurvival Bcl-2 proteins activates Bax/Bak through the outer mitochondrial membrane. Genes Dev. 2016;30:973-88 pubmed publisher
  9. Gauthier T, Claude Taupin A, Delage Mourroux R, Boyer Guittaut M, Hervouet E. Proximity Ligation In situ Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction. PLoS ONE. 2015;10:e0128701 pubmed publisher
  10. Huang Z, Han Z, Ye B, Dai Z, Shan P, Lu Z, et al. Berberine alleviates cardiac ischemia/reperfusion injury by inhibiting excessive autophagy in cardiomyocytes. Eur J Pharmacol. 2015;762:1-10 pubmed publisher
  11. Shi R, Zhu S, Li V, Gibson S, Xu X, Kong J. BNIP3 interacting with LC3 triggers excessive mitophagy in delayed neuronal death in stroke. CNS Neurosci Ther. 2014;20:1045-55 pubmed publisher