This is a Validated Antibody Database (VAD) review about mouse Nedd4, based on 6 published articles (read how Labome selects the articles), using Nedd4 antibody in all methods. It is aimed to help Labome visitors find the most suited Nedd4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Nedd4 synonym: AA959633; AL023035; AU019897; E430025J12Rik; EG639396; Gm7265; KIAA0093; Nedd4-1; Nedd4a

Knockout validation
BD Biosciences
mouse monoclonal (15/Nedd4)
  • western blot knockout validation; mouse; 1:3000; fig 4c
BD Biosciences Nedd4 antibody (BD, 611481) was used in western blot knockout validation on mouse samples at 1:3000 (fig 4c). Nat Commun (2022) ncbi
BD Biosciences
mouse monoclonal (15/Nedd4)
  • western blot; human; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 2c
BD Biosciences Nedd4 antibody (BD Biosciences, 611481) was used in western blot on human samples (fig 2a) and in western blot knockout validation on mouse samples (fig 2c). Autophagy (2017) ncbi
BD Biosciences
mouse monoclonal (15/Nedd4)
  • immunocytochemistry knockout validation; mouse; fig 3
  • western blot knockout validation; mouse; fig 1
BD Biosciences Nedd4 antibody (BD Biosciences, 611480) was used in immunocytochemistry knockout validation on mouse samples (fig 3) and in western blot knockout validation on mouse samples (fig 1). PLoS ONE (2012) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 7e
  • immunoprecipitation; human; 1:100; loading ...; fig 2b
  • immunohistochemistry; human; 1:100; loading ...; fig 7e
  • western blot; human; 1:1000; loading ...; fig 2b, 4f
Invitrogen Nedd4 antibody (Thermo, PA5-17463) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 7e), in immunoprecipitation on human samples at 1:100 (fig 2b), in immunohistochemistry on human samples at 1:100 (fig 7e) and in western blot on human samples at 1:1000 (fig 2b, 4f). Nat Commun (2020) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
In order to report that DUB3 family deubiquitylating enzymes regulate Hippo pathway activity, Cell Signaling Technology Nedd4 antibody (Cell Signaling, 2740) was used in western blot on human samples (fig 2a). PLoS ONE (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1c
In order to elucidate how Cdh1 regulates NEDD4 family E3 ligases such as WWP2, Cell Signaling Technology Nedd4 antibody (Cell Signaling, 2740) was used in western blot on human samples (fig 1c). Cell Discov (2016) ncbi
BD Biosciences
mouse monoclonal (15/Nedd4)
  • western blot knockout validation; mouse; 1:3000; fig 4c
BD Biosciences Nedd4 antibody (BD, 611481) was used in western blot knockout validation on mouse samples at 1:3000 (fig 4c). Nat Commun (2022) ncbi
mouse monoclonal (15/Nedd4)
  • western blot; human; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 2c
BD Biosciences Nedd4 antibody (BD Biosciences, 611481) was used in western blot on human samples (fig 2a) and in western blot knockout validation on mouse samples (fig 2c). Autophagy (2017) ncbi
mouse monoclonal (15/Nedd4)
  • immunocytochemistry knockout validation; mouse; fig 3
  • western blot knockout validation; mouse; fig 1
BD Biosciences Nedd4 antibody (BD Biosciences, 611480) was used in immunocytochemistry knockout validation on mouse samples (fig 3) and in western blot knockout validation on mouse samples (fig 1). PLoS ONE (2012) ncbi
Articles Reviewed
  1. Lohraseb I, McCarthy P, Secker G, Marchant C, Wu J, Ali N, et al. Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells. Nat Commun. 2022;13:2018 pubmed publisher
  2. Yang Y, Luo M, Zhang K, Zhang J, Gao T, Connell D, et al. Nedd4 ubiquitylates VDAC2/3 to suppress erastin-induced ferroptosis in melanoma. Nat Commun. 2020;11:433 pubmed publisher
  3. Pei G, Buijze H, Liu H, Moura Alves P, Goosmann C, Brinkmann V, et al. The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy. Autophagy. 2017;13:2041-2055 pubmed publisher
  4. Nguyen H, Kugler J, Cohen S. DUB3 Deubiquitylating Enzymes Regulate Hippo Pathway Activity by Regulating the Stability of ITCH, LATS and AMOT Proteins. PLoS ONE. 2017;12:e0169587 pubmed publisher
  5. Liu J, Wan L, Liu J, Yuan Z, Zhang J, Guo J, et al. Cdh1 inhibits WWP2-mediated ubiquitination of PTEN to suppress tumorigenesis in an APC-independent manner. Cell Discov. 2016;2:15044 pubmed publisher
  6. Nagpal P, Plant P, Correa J, Bain A, Takeda M, Kawabe H, et al. The ubiquitin ligase Nedd4-1 participates in denervation-induced skeletal muscle atrophy in mice. PLoS ONE. 2012;7:e46427 pubmed publisher