This is a Validated Antibody Database (VAD) review about mouse Ncan, based on 6 published articles (read how Labome selects the articles), using Ncan antibody in all methods. It is aimed to help Labome visitors find the most suited Ncan antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ncan synonym: C230035B04; Cspg3; Cspg3-rs; Tgfbit; neurocan core protein; TGFbeta-induced transcript, brain; chondroitin sulfate proteoglycan 3, related sequence

R&D Systems
sheep polyclonal
  • immunohistochemistry - frozen section; mouse; 1:25; loading ...; fig 8f
In order to characterize parvalbumin interneurons and perineuronal nets in the prefrontal cortex, R&D Systems Ncan antibody (R&D systems, AF5800) was used in immunohistochemistry - frozen section on mouse samples at 1:25 (fig 8f). Neuroscience (2017) ncbi
sheep polyclonal
  • immunohistochemistry; mouse; 1:200; fig 5
  • western blot; mouse; 1:3000; fig 1
R&D Systems Ncan antibody (R&D Systems, AF5800) was used in immunohistochemistry on mouse samples at 1:200 (fig 5) and in western blot on mouse samples at 1:3000 (fig 1). Neural Plast (2016) ncbi
sheep polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; fig 8
In order to test if bone morphogenetic proteins trigger reactive gliosis in retinal astrocytes and Muller glial cells, R&D Systems Ncan antibody (R&D Systems, AF5800) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 8). Mol Vis (2014) ncbi
sheep polyclonal
  • immunohistochemistry - frozen section; rabbit
  • western blot; rabbit
  • immunohistochemistry - frozen section; human
R&D Systems Ncan antibody (R & D, AF5800) was used in immunohistochemistry - frozen section on rabbit samples , in western blot on rabbit samples and in immunohistochemistry - frozen section on human samples . Exp Neurol (2013) ncbi
sheep polyclonal
  • immunohistochemistry - free floating section; mouse; 1:200
  • western blot; mouse
R&D Systems Ncan antibody (R&D systems, BAF5800) was used in immunohistochemistry - free floating section on mouse samples at 1:200 and in western blot on mouse samples . J Comp Neurol (2012) ncbi
sheep polyclonal
  • immunohistochemistry; mouse; 1:25
  • western blot; mouse; 1:100
R&D Systems Ncan antibody (R&D Systems, AF5800) was used in immunohistochemistry on mouse samples at 1:25 and in western blot on mouse samples at 1:100. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. Ueno H, Suemitsu S, Okamoto M, Matsumoto Y, Ishihara T. Parvalbumin neurons and perineuronal nets in the mouse prefrontal cortex. Neuroscience. 2017;343:115-127 pubmed publisher
  2. Miyata S, Kitagawa H. Chondroitin 6-Sulfation Regulates Perineuronal Net Formation by Controlling the Stability of Aggrecan. Neural Plast. 2016;2016:1305801 pubmed publisher
  3. Dharmarajan S, Gurel Z, Wang S, Sorenson C, Sheibani N, Belecky Adams T. Bone morphogenetic protein 7 regulates reactive gliosis in retinal astrocytes and Müller glia. Mol Vis. 2014;20:1085-108 pubmed
  4. Vinukonda G, Zia M, Bhimavarapu B, Hu F, Feinberg M, Bokhari A, et al. Intraventricular hemorrhage induces deposition of proteoglycans in premature rabbits, but their in vivo degradation with chondroitinase does not restore myelination, ventricle size and neurological recovery. Exp Neurol. 2013;247:630-44 pubmed publisher
  5. Yi J, Katagiri Y, Susarla B, Figge D, Symes A, Geller H. Alterations in sulfated chondroitin glycosaminoglycans following controlled cortical impact injury in mice. J Comp Neurol. 2012;520:3295-313 pubmed publisher
  6. Bekku Y, Saito M, Moser M, Fuchigami M, Maehara A, Nakayama M, et al. Bral2 is indispensable for the proper localization of brevican and the structural integrity of the perineuronal net in the brainstem and cerebellum. J Comp Neurol. 2012;520:1721-36 pubmed publisher