This is a Validated Antibody Database (VAD) review about mouse Muc1, based on 22 published articles (read how Labome selects the articles), using Muc1 antibody in all methods. It is aimed to help Labome visitors find the most suited Muc1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Muc1 synonym: CD227; EMA; Muc-1; mucin-1; episialin

Knockout validation
Invitrogen
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry; human; fig 1c
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2d
  • western blot knockout validation; mouse; loading ...; fig 2b
In order to test if MUC1 contributes to epithelial tubular cell plasticity and kidney fibrosis, Invitrogen Muc1 antibody (ThermoFisher, MA5-11202) was used in immunohistochemistry on human samples (fig 1c), in immunohistochemistry knockout validation on mouse samples (fig 2d) and in western blot knockout validation on mouse samples (fig 2b). Biochim Biophys Acta Mol Basis Dis (2017) ncbi
Invitrogen
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry; human; fig 1c
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2d
  • western blot knockout validation; mouse; loading ...; fig 2b
In order to test if MUC1 contributes to epithelial tubular cell plasticity and kidney fibrosis, Invitrogen Muc1 antibody (ThermoFisher, MA5-11202) was used in immunohistochemistry on human samples (fig 1c), in immunohistochemistry knockout validation on mouse samples (fig 2d) and in western blot knockout validation on mouse samples (fig 2b). Biochim Biophys Acta Mol Basis Dis (2017) ncbi
hamsters monoclonal (MH1 (CT2))
  • western blot; human; loading ...; fig 2
In order to determine effects of the MUC1-C subunit inhibitor (GO-203) on human colorectal cancer cells, Invitrogen Muc1 antibody (Thermo Scientific, Ab5) was used in western blot on human samples (fig 2). Mol Cancer (2017) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry - paraffin section; human; 1:50; fig 4
In order to study a case report and literature review of recurrent intracranial neurenteric cyst with malignant transformation, Invitrogen Muc1 antibody (Thermo Fisher, MA5-11202) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 4). Oncol Lett (2016) ncbi
hamsters monoclonal (MH1 (CT2))
  • chromatin immunoprecipitation; human; loading ...; fig 3b
  • immunohistochemistry; human; 1:200; fig 6b
  • western blot; human; loading ...; fig 1b
In order to determine that MUC1 stimulates epidermal growth factor receptor expression and function in endometrial cancer, Invitrogen Muc1 antibody (ProSci, MH1) was used in chromatin immunoprecipitation on human samples (fig 3b), in immunohistochemistry on human samples at 1:200 (fig 6b) and in western blot on human samples (fig 1b). Oncotarget (2016) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 7
In order to study the contribution of hematopoietically expressed homeobox protein in ductal secretion and pancreatitis, Invitrogen Muc1 antibody (NeoMarkers, 1630X1210A) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 7). Cell Mol Gastroenterol Hepatol (2015) ncbi
mouse monoclonal (GP1.4)
  • western blot; human; loading ...; fig 1f
In order to investigate the role of MUC1 on macrophage differentiation or phagocytic activity, Invitrogen Muc1 antibody (Pierce, GP1.4) was used in western blot on human samples (fig 1f). Am J Respir Cell Mol Biol (2016) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry - paraffin section; human; 1:200
In order to determine if miRNA that target Muc1 are protective against pancreatic cancer, Invitrogen Muc1 antibody (Neomarkers Labvision, HM-1630P1) was used in immunohistochemistry - paraffin section on human samples at 1:200. Biochim Biophys Acta (2015) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry - paraffin section; mouse; 1:200
In order to investigate the role of transcription factors SOX4 and SOX9 during the development of bile ducts, Invitrogen Muc1 antibody (Neomarkers, HM-1630-P) was used in immunohistochemistry - paraffin section on mouse samples at 1:200. Dev Biol (2015) ncbi
mouse monoclonal (GP1.4)
  • immunohistochemistry - paraffin section; human; fig 5
  • flow cytometry; human; fig 1
  • western blot; human; fig 1
In order to study the shift of Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway occurs by Mucin1 activating JNK in human hepatocellular carcinoma cells, Invitrogen Muc1 antibody (Neomarker, GP1.4) was used in immunohistochemistry - paraffin section on human samples (fig 5), in flow cytometry on human samples (fig 1) and in western blot on human samples (fig 1). Oncotarget (2015) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 3
In order to study the shift of Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway occurs by Mucin1 activating JNK in human hepatocellular carcinoma cells, Invitrogen Muc1 antibody (Neomarker, Ab-5) was used in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 3). Oncotarget (2015) ncbi
mouse monoclonal (GP1.4)
  • immunohistochemistry; human; 1:400
In order to describe a tumor from a patient with oncocytic cystadenoma, Invitrogen Muc1 antibody (Thermo Scientific, GP1.4) was used in immunohistochemistry on human samples at 1:400. Medicine (Baltimore) (2014) ncbi
mouse monoclonal (GP1.4)
  • immunohistochemistry - paraffin section; human
In order to compare meningiomas and mesotheliomas immunohistochemically, Invitrogen Muc1 antibody (Thermo Scientific, GP1.4) was used in immunohistochemistry - paraffin section on human samples . Biomed Res Int (2014) ncbi
hamsters monoclonal (MH1 (CT2))
In order to examine the anti-tumor potential of BCR-ABL disruption and secondary leukemia-specific pathway inhibitors, Invitrogen Muc1 antibody (Thermo Scientific, MUC-1 Ab-5/HM-1630) was used . Mol Pharm (2013) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry - paraffin section; mouse; 1:300
In order to study the roles of GATA4 and GATA6 in mouse pancreas organogenesis, Invitrogen Muc1 antibody (Thermo, HM-1630,) was used in immunohistochemistry - paraffin section on mouse samples at 1:300. J Clin Invest (2012) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunocytochemistry; human; 0.67 ug/ml; fig 7
  • western blot; human; 0.04 ug/ml
  • immunohistochemistry; Rhesus monkey; 0.4 ug/ml; fig 2
  • western blot; African green monkey; 0.04 ug/ml
In order to determine if MUC1-N is expressed inside the nucleus, Invitrogen Muc1 antibody (Thermo Fisher, HM-1630-P0) was used in immunocytochemistry on human samples at 0.67 ug/ml (fig 7), in western blot on human samples at 0.04 ug/ml, in immunohistochemistry on Rhesus monkey samples at 0.4 ug/ml (fig 2) and in western blot on African green monkey samples at 0.04 ug/ml. PLoS ONE (2012) ncbi
hamsters monoclonal (MH1 (CT2))
  • immunohistochemistry - frozen section; mouse; 1:500; fig 1
In order to investigate the origins of new beta-cells, Invitrogen Muc1 antibody (NeoMarkers, HM-1630-P1) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1). BMC Dev Biol (2010) ncbi
Abcam
rabbit monoclonal (EPR1023)
  • flow cytometry; human; loading ...; fig s6
In order to investigate origin of tumor-infiltrating T regulatory cells in breast cancer samples, Abcam Muc1 antibody (Abcam, ab109185) was used in flow cytometry on human samples (fig s6). Cell Res (2017) ncbi
mouse monoclonal (HMFG1 (aka 1.10.F3))
  • immunocytochemistry; human; 1:200; loading ...; fig 1c
  • western blot; human; 1:500; loading ...; fig 1a
In order to explore the contribution of TRPC5 carrying exosomes to breast cancer, Abcam Muc1 antibody (Abcam, ab70475) was used in immunocytochemistry on human samples at 1:200 (fig 1c) and in western blot on human samples at 1:500 (fig 1a). Cancer Sci (2017) ncbi
rabbit monoclonal (EP1024Y)
  • western blot; human; 1:1000; loading ...; fig 4a
Abcam Muc1 antibody (Abcam, ab45167) was used in western blot on human samples at 1:1000 (fig 4a). Int J Cancer (2017) ncbi
(MH1)
  • western blot; mouse; fig 1d
  • immunoprecipitation; human; fig 2d
  • western blot; human; fig 1a,1b
Abcam Muc1 antibody (Abcam, ab80952) was used in western blot on mouse samples (fig 1d), in immunoprecipitation on human samples (fig 2d) and in western blot on human samples (fig 1a,1b). Oncotarget (2016) ncbi
(MH1)
  • chromatin immunoprecipitation; human; loading ...; fig 3b
  • immunohistochemistry; human; 1:200; fig 6b
  • western blot; human; loading ...; fig 1b
In order to determine that MUC1 stimulates epidermal growth factor receptor expression and function in endometrial cancer, Abcam Muc1 antibody (ProSci, MH1) was used in chromatin immunoprecipitation on human samples (fig 3b), in immunohistochemistry on human samples at 1:200 (fig 6b) and in western blot on human samples (fig 1b). Oncotarget (2016) ncbi
rabbit monoclonal (EP1024Y)
  • immunohistochemistry; rat; 1:200; fig 1b
In order to study intra-abdominal hypertension-induced changes in gastrointestinal permeability, Abcam Muc1 antibody (Abcam, ab45167) was used in immunohistochemistry on rat samples at 1:200 (fig 1b). Sci Rep (2016) ncbi
rabbit monoclonal (EP1024Y)
  • immunohistochemistry - paraffin section; human; 1:200
Abcam Muc1 antibody (Abcam, ab45167) was used in immunohistochemistry - paraffin section on human samples at 1:200. Exp Mol Pathol (2014) ncbi
rabbit monoclonal (EPR1023)
  • western blot; human
Abcam Muc1 antibody (Epitomics, 2900-1) was used in western blot on human samples . Oncogene (2015) ncbi
Articles Reviewed
  1. Gibier J, Hemon B, Fanchon M, Gaudelot K, Pottier N, Ringot B, et al. Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase. Biochim Biophys Acta Mol Basis Dis. 2017;1863:1336-1349 pubmed publisher
  2. Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
  3. Ahmad R, Alam M, Hasegawa M, Uchida Y, Al Obaid O, Kharbanda S, et al. Targeting MUC1-C inhibits the AKT-S6K1-elF4A pathway regulating TIGAR translation in colorectal cancer. Mol Cancer. 2017;16:33 pubmed publisher
  4. Wang T, Ning K, Lu T, Sun X, Jin L, Qi X, et al. Increasing circulating exosomes-carrying TRPC5 predicts chemoresistance in metastatic breast cancer patients. Cancer Sci. 2017;108:448-454 pubmed publisher
  5. Tang Z, Li J, Shen Q, Feng J, Liu H, Wang W, et al. Contribution of upregulated dipeptidyl peptidase 9 (DPP9) in promoting tumoregenicity, metastasis and the prediction of poor prognosis in non-small cell lung cancer (NSCLC). Int J Cancer. 2017;140:1620-1632 pubmed publisher
  6. Hanson R, Brown R, Steele M, Grandgenett P, Grunkemeyer J, Hollingsworth M. Identification of FRA-1 as a novel player in pancreatic cancer in cooperation with a MUC1: ERK signaling axis. Oncotarget. 2016;7:39996-40011 pubmed publisher
  7. Yang Y, Fang J, Li D, Wang L, Ji N, Zhang J. Recurrent intracranial neurenteric cyst with malignant transformation: A case report and literature review. Oncol Lett. 2016;11:3395-3402 pubmed
  8. Engel B, Bowser J, Broaddus R, Carson D. MUC1 stimulates EGFR expression and function in endometrial cancer. Oncotarget. 2016;7:32796-809 pubmed publisher
  9. Leng Y, Yi M, Fan J, Bai Y, Ge Q, Yao G. Effects of acute intra-abdominal hypertension on multiple intestinal barrier functions in rats. Sci Rep. 2016;6:22814 pubmed publisher
  10. Ferreira M, McKenna L, Zhang J, Reichert M, Bakir B, Buza E, et al. Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice. Cell Mol Gastroenterol Hepatol. 2015;1:550-569 pubmed publisher
  11. Kato K, Uchino R, Lillehoj E, Knox K, Lin Y, Kim K. Membrane-Tethered MUC1 Mucin Counter-Regulates the Phagocytic Activity of Macrophages. Am J Respir Cell Mol Biol. 2016;54:515-23 pubmed publisher
  12. Tréhoux S, Lahdaoui F, Delpu Y, Renaud F, Leteurtre E, Torrisani J, et al. Micro-RNAs miR-29a and miR-330-5p function as tumor suppressors by targeting the MUC1 mucin in pancreatic cancer cells. Biochim Biophys Acta. 2015;1853:2392-403 pubmed publisher
  13. Poncy A, Antoniou A, Cordi S, Pierreux C, Jacquemin P, Lemaigre F. Transcription factors SOX4 and SOX9 cooperatively control development of bile ducts. Dev Biol. 2015;404:136-48 pubmed publisher
  14. Li Q, Liu G, Yuan H, Wang J, Guo Y, Chen T, et al. Mucin1 shifts Smad3 signaling from the tumor-suppressive pSmad3C/p21(WAF1) pathway to the oncogenic pSmad3L/c-Myc pathway by activating JNK in human hepatocellular carcinoma cells. Oncotarget. 2015;6:4253-65 pubmed
  15. BaÅŸak K, KiroÄŸlu K. Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report. Medicine (Baltimore). 2014;93:e246 pubmed publisher
  16. Abdelzaher E, Abdallah D. Expression of mesothelioma-related markers in meningiomas: an immunohistochemical study. Biomed Res Int. 2014;2014:968794 pubmed publisher
  17. Sørdal Ø, Qvigstad G, Nordrum I, Sandvik A, Gustafsson B, Waldum H. The PAS positive material in gastric cancer cells of signet ring type is not mucin. Exp Mol Pathol. 2014;96:274-8 pubmed publisher
  18. Cyr A, Kulak M, Park J, Bogachek M, Spanheimer P, Woodfield G, et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene. 2015;34:436-44 pubmed publisher
  19. Woessner D, Lim C. Disrupting BCR-ABL in combination with secondary leukemia-specific pathways in CML cells leads to enhanced apoptosis and decreased proliferation. Mol Pharm. 2013;10:270-7 pubmed publisher
  20. Carrasco M, Delgado I, Soria B, Martin F, Rojas A. GATA4 and GATA6 control mouse pancreas organogenesis. J Clin Invest. 2012;122:3504-15 pubmed publisher
  21. Kumar P, Kumar P, Lindberg L, Thirkill T, Ji J, Martsching L, et al. The MUC1 extracellular domain subunit is found in nuclear speckles and associates with spliceosomes. PLoS ONE. 2012;7:e42712 pubmed publisher
  22. Kopinke D, Murtaugh L. Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas. BMC Dev Biol. 2010;10:38 pubmed publisher