This is a Validated Antibody Database (VAD) review about mouse Mlkl, based on 37 published articles (read how Labome selects the articles), using Mlkl antibody in all methods. It is aimed to help Labome visitors find the most suited Mlkl antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Mlkl synonym: 9130019I15Rik

Knockout validation
Abcam
domestic rabbit monoclonal (EPR9515(2))
  • immunohistochemistry knockout validation; mouse; fig 1
  • immunohistochemistry - paraffin section; mouse; fig 6
  • western blot; mouse; fig 1
In order to study protection of mice from high fat diet-induced liver injury by receptor interacting protein 3, Abcam Mlkl antibody (Abcam, ab196436) was used in immunohistochemistry knockout validation on mouse samples (fig 1), in immunohistochemistry - paraffin section on mouse samples (fig 6) and in western blot on mouse samples (fig 1). Hepatology (2016) ncbi
Cell Signaling Technology
monoclonal (D6W1K)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s1c
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1c
Cell Signaling Technology Mlkl antibody (Cell Signaling, 37705) was used in western blot knockout validation on mouse samples at 1:1000 (fig s1c) and in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 1c). Proc Natl Acad Sci U S A (2022) ncbi
others
monoclonal (7C6.1)
  • western blot; mouse; 1:500; loading ...; fig 1j, 5e, 6d
 Mlkl antibody (Merck, MABC1158) was used in western blot on mouse samples at 1:500 (fig 1j, 5e, 6d). Nat Commun (2018) ncbi
Abcam
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; 1:1000; loading ...; fig 3b, 3g
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples at 1:1000 (fig 3b, 3g). Cell Death Discov (2022) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s5c
  • western blot; mouse; 1:1000; loading ...; fig 4b
Abcam Mlkl antibody (Abcam, ab196436) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s5c) and in western blot on mouse samples at 1:1000 (fig 4b). Stem Cell Reports (2021) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • immunohistochemistry; rat; 1:2000; loading ...; fig 1g
Abcam Mlkl antibody (Abcam, ab196436) was used in immunohistochemistry on rat samples at 1:2000 (fig 1g). J Neuroinflammation (2021) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 4b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 4b). Front Med (Lausanne) (2021) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; human; 1:1000; loading ...; fig 5g
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on human samples at 1:1000 (fig 5g). Cancers (Basel) (2021) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 3a
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 3a). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; 1:1000; loading ...; fig s2b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples at 1:1000 (fig s2b). Cell Death Dis (2021) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 2c, 6b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 2c, 6b). Nat Commun (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 4g
  • western blot; mouse; loading ...; fig 4i
Abcam Mlkl antibody (Abcam, ab196436) was used in immunohistochemistry on mouse samples at 1:1000 (fig 4g) and in western blot on mouse samples (fig 4i). elife (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • immunocytochemistry; mouse; loading ...; fig 2c
Abcam Mlkl antibody (Abcam, ab196436) was used in immunocytochemistry on mouse samples (fig 2c). Cell Res (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; 1:1000; loading ...; fig 4b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples at 1:1000 (fig 4b). elife (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; 1:6000; loading ...; fig 3b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples at 1:6000 (fig 3b). Cell Death Dis (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig s3a
  • western blot; mouse; 1:1000; loading ...; fig 3f
Abcam Mlkl antibody (Abcam, ab196436) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig s3a) and in western blot on mouse samples at 1:1000 (fig 3f). Cell Death Dis (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
Abcam Mlkl antibody (Abcam, ab196436) was used . Nat Commun (2020) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 4s1a
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 4s1a). elife (2019) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; 1:1000; loading ...; fig 3b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples at 1:1000 (fig 3b). J Cell Sci (2019) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; human; 1:500; loading ...; fig 2b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on human samples at 1:500 (fig 2b). Cell Death Differ (2019) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 5b
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 5b). Toxicology (2019) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; fig s7a
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig s7a). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 2c
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 2c). Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 6a
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 6a). J Cell Biol (2017) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 1c
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 1c). EMBO J (2017) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; loading ...; fig 8a
Abcam Mlkl antibody (Abcam, EPR9515(2)) was used in western blot on mouse samples (fig 8a). Infect Immun (2017) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; 1:2000; fig S6
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples at 1:2000 (fig S6). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; human; fig 5
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on human samples (fig 5). EMBO J (2016) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • immunohistochemistry knockout validation; mouse; fig 1
  • immunohistochemistry - paraffin section; mouse; fig 6
  • western blot; mouse; fig 1
In order to study protection of mice from high fat diet-induced liver injury by receptor interacting protein 3, Abcam Mlkl antibody (Abcam, ab196436) was used in immunohistochemistry knockout validation on mouse samples (fig 1), in immunohistochemistry - paraffin section on mouse samples (fig 6) and in western blot on mouse samples (fig 1). Hepatology (2016) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; fig 5
Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 5). Cell Rep (2016) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; fig 4
In order to analyze induction of efficient anti-tumor immunity by vaccination with necroptotic cancer cells, Abcam Mlkl antibody (Abcam, ab196436) was used in western blot on mouse samples (fig 4). Cell Rep (2016) ncbi
domestic rabbit monoclonal (EPR9515(2))
  • western blot; mouse; fig 7
Abcam Mlkl antibody (Abcam, 196436) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
Novus Biologicals
domestic rabbit monoclonal (JM92-37)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 3h
  • western blot; mouse; 1:750; loading ...; fig 2a, 3d
Novus Biologicals Mlkl antibody (Novus, NBP2-66953) was used in immunocytochemistry on mouse samples at 1:100 (fig 3h) and in western blot on mouse samples at 1:750 (fig 2a, 3d). Stem Cells Dev (2020) ncbi
domestic rabbit monoclonal (JM92-37)
  • immunohistochemistry - paraffin section; mouse; fig 5c
  • western blot; mouse; loading ...; fig 5a
Novus Biologicals Mlkl antibody (Novus Biologicals, NBP2?\66953) was used in immunohistochemistry - paraffin section on mouse samples (fig 5c) and in western blot on mouse samples (fig 5a). Oral Dis (2020) ncbi
Cell Signaling Technology
monoclonal (D6W1K)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s1c
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1c
Cell Signaling Technology Mlkl antibody (Cell Signaling, 37705) was used in western blot knockout validation on mouse samples at 1:1000 (fig s1c) and in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 1c). Proc Natl Acad Sci U S A (2022) ncbi
monoclonal (D6W1K)
  • western blot; mouse; 1:1000; loading ...; fig s8a
Cell Signaling Technology Mlkl antibody (CST, 37705) was used in western blot on mouse samples at 1:1000 (fig s8a). Nat Commun (2021) ncbi
monoclonal (D6W1K)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Cell Signaling Technology Mlkl antibody (Cell Signaling Technology, 37705) was used in western blot on mouse samples at 1:1000 (fig 4b). elife (2020) ncbi
monoclonal (D6W1K)
  • western blot; mouse; loading ...; fig 3b
Cell Signaling Technology Mlkl antibody (Cell Signaling, 37705) was used in western blot on mouse samples (fig 3b). J Mol Cell Cardiol (2018) ncbi
Abcepta
  • western blot; mouse; loading ...; fig s3e
Abcepta Mlkl antibody (Abgent, AP14272b) was used in western blot on mouse samples (fig s3e). Cell (2021) ncbi
  • immunocytochemistry; mouse; loading ...
  • western blot; mouse; loading ...
Abcepta Mlkl antibody (Abgent, AP14272b) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . Cell Res (2020) ncbi
  • western blot; mouse; fig s7a
Abcepta Mlkl antibody (Abgent, ap14272b) was used in western blot on mouse samples (fig s7a). J Clin Invest (2018) ncbi
  • immunocytochemistry; mouse
Abcepta Mlkl antibody (Abgent, AP14272b) was used in immunocytochemistry on mouse samples . Cell Death Differ (2016) ncbi
Articles Reviewed
  1. Guo J, Guo Z, Huang Y, Ma S, Yan B, Pan C, et al. Blockage of MLKL prevents myelin damage in experimental diabetic neuropathy. Proc Natl Acad Sci U S A. 2022;119:e2121552119 pubmed publisher
  2. Wang J, Li X, Wang Y, Li Y, Shi F, Diao H. Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis. Cell Death Discov. 2022;8:97 pubmed publisher
  3. Supper E, Rudat S, Iyer V, Droop A, Wong K, Spinella J, et al. Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Nat Commun. 2021;12:2482 pubmed publisher
  4. Wang S, Li S, Li Y, Jiang Q, Li X, Wang Y, et al. Non-muscle myosin heavy chain 9 maintains intestinal homeostasis by preventing epithelium necroptosis and colitis adenoma formation. Stem Cell Reports. 2021;16:1290-1301 pubmed publisher
  5. Zhan L, Lu X, Xu W, Sun W, Xu E. Inhibition of MLKL-dependent necroptosis via downregulating interleukin-1R1 contributes to neuroprotection of hypoxic preconditioning in transient global cerebral ischemic rats. J Neuroinflammation. 2021;18:97 pubmed publisher
  6. Ngamsri K, Gamper Tsigaras J, Reutershan J, Konrad F. Fractalkine Is Linked to the Necrosome Pathway in Acute Pulmonary Inflammation. Front Med (Lausanne). 2021;8:591790 pubmed publisher
  7. Zheng Z, Yang P, Luo W, Yu S, Xu H, Huang Y, et al. STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma. Cancers (Basel). 2021;13: pubmed publisher
  8. Moujalled D, Gangatirkar P, Kauppi M, Corbin J, Lebois M, Murphy J, et al. The necroptotic cell death pathway operates in megakaryocytes, but not in platelet synthesis. Cell Death Dis. 2021;12:133 pubmed publisher
  9. Chen C, Wang D, Yu Y, Zhao T, Min N, Wu Y, et al. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI. Cell Death Dis. 2021;12:65 pubmed publisher
  10. Li X, Zhang M, Huang X, Liang W, Li G, Lu X, et al. Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun. 2020;11:6364 pubmed publisher
  11. Karki R, Sharma B, Tuladhar S, Williams E, Zalduondo L, Samir P, et al. Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes. Cell. 2021;184:149-168.e17 pubmed publisher
  12. Li D, Ai Y, Guo J, Dong B, Li L, Cai G, et al. Casein kinase 1G2 suppresses necroptosis-promoted testis aging by inhibiting receptor-interacting kinase 3. elife. 2020;9: pubmed publisher
  13. Zhou S, Zhang W, Cai G, Ding Y, Wei C, Li S, et al. Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration. Cell Res. 2020;30:1063-1077 pubmed publisher
  14. Kim E, Woodruff M, Grigoryan L, Maier B, Lee S, Mandal P, et al. Squalene emulsion-based vaccine adjuvants stimulate CD8 T cell, but not antibody responses, through a RIPK3-dependent pathway. elife. 2020;9: pubmed publisher
  15. Wu Y, Chou T, Young L, Hsieh F, Pan H, Mo S, et al. Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis. 2020;11:305 pubmed publisher
  16. Duan X, Liu X, Liu N, Huang Y, Jin Z, Zhang S, et al. Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation. Cell Death Dis. 2020;11:134 pubmed publisher
  17. Xie Y, Chen H, Luo D, Yang X, Yao J, Zhang C, et al. Inhibiting Necroptosis of Spermatogonial Stem Cell as a Novel Strategy for Male Fertility Preservation. Stem Cells Dev. 2020;29:475-487 pubmed publisher
  18. Tanaka H, Homma H, Fujita K, Kondo K, Yamada S, Jin X, et al. YAP-dependent necrosis occurs in early stages of Alzheimer's disease and regulates mouse model pathology. Nat Commun. 2020;11:507 pubmed publisher
  19. Choi S, Shin S, Lee H, Sohn K, Yoon S, Kim J. 1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol ameliorates chemoradiation-induced oral mucositis. Oral Dis. 2020;26:111-121 pubmed publisher
  20. Lim J, Park H, Heisler J, Maculins T, Roose Girma M, Xu M, et al. Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins. elife. 2019;8: pubmed publisher
  21. Wang Z, Feng J, Yu J, Chen G. FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation. J Cell Sci. 2019;132: pubmed publisher
  22. Xiong Y, Li L, Zhang L, Cui Y, Wu C, Li H, et al. The bromodomain protein BRD4 positively regulates necroptosis via modulating MLKL expression. Cell Death Differ. 2019;: pubmed publisher
  23. Yuan Z, Zhang H, Hasnat M, Ding J, Chen X, Liang P, et al. A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation. Toxicology. 2019;414:45-56 pubmed publisher
  24. Benmerzoug S, Rose S, Bounab B, Gosset D, Duneau L, Chenuet P, et al. STING-dependent sensing of self-DNA drives silica-induced lung inflammation. Nat Commun. 2018;9:5226 pubmed publisher
  25. Yang Z, Li C, Wang Y, Yang J, Yin Y, Liu M, et al. Melatonin attenuates chronic pain related myocardial ischemic susceptibility through inhibiting RIP3-MLKL/CaMKII dependent necroptosis. J Mol Cell Cardiol. 2018;125:185-194 pubmed publisher
  26. Li F, Li Y, Liang H, Xu T, Kong Y, Huang M, et al. HECTD3 mediates TRAF3 polyubiquitination and type I interferon induction during bacterial infection. J Clin Invest. 2018;128:4148-4162 pubmed publisher
  27. Meng H, Liu Z, Li X, Wang H, Jin T, Wu G, et al. Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis. Proc Natl Acad Sci U S A. 2018;115:E2001-E2009 pubmed publisher
  28. Hos N, Ganesan R, Gutierrez S, Hos D, Klimek J, Abdullah Z, et al. Type I interferon enhances necroptosis of Salmonella Typhimurium-infected macrophages by impairing antioxidative stress responses. J Cell Biol. 2017;216:4107-4121 pubmed publisher
  29. Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
  30. Günster R, Matthews S, Holden D, Thurston T. SseK1 and SseK3 Type III Secretion System Effectors Inhibit NF-κB Signaling and Necroptotic Cell Death in Salmonella-Infected Macrophages. Infect Immun. 2017;85: pubmed publisher
  31. Gautheron J, Vucur M, Schneider A, Severi I, Roderburg C, Roy S, et al. The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance. Nat Commun. 2016;7:11869 pubmed publisher
  32. Wagner S, Satpathy S, Beli P, Choudhary C. SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes. EMBO J. 2016;35:1868-84 pubmed publisher
  33. Roychowdhury S, McCullough R, Sanz Garcia C, Saikia P, Alkhouri N, Matloob A, et al. Receptor interacting protein 3 protects mice from high-fat diet-induced liver injury. Hepatology. 2016;64:1518-1533 pubmed publisher
  34. Tortola L, Nitsch R, Bertrand M, Kogler M, Redouane Y, Kozieradzki I, et al. The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate. Cell Rep. 2016;15:1481-1492 pubmed publisher
  35. Aaes T, Kaczmarek A, Delvaeye T, De Craene B, De Koker S, Heyndrickx L, et al. Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep. 2016;15:274-87 pubmed publisher
  36. Thornton T, Delgado P, Chen L, Salas B, Krementsov D, Fernández M, et al. Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response. Nat Commun. 2016;7:10553 pubmed publisher
  37. Yoon S, Bogdanov K, Kovalenko A, Wallach D. Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it. Cell Death Differ. 2016;23:253-60 pubmed publisher