This is a Validated Antibody Database (VAD) review about mouse Mitf, based on 25 published articles (read how Labome selects the articles), using Mitf antibody in all methods. It is aimed to help Labome visitors find the most suited Mitf antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Mitf synonym: BCC2; Bhlhe32; Gsfbcc2; Vitiligo; Wh; bw; mi; vit

Knockout validation
Abcam
mouse monoclonal (C5)
  • western blot knockout validation; human; 1:2000; loading ...; fig s1-1a
Abcam Mitf antibody (Abcam, ab12039) was used in western blot knockout validation on human samples at 1:2000 (fig s1-1a). elife (2021) ncbi
Abcam
mouse monoclonal (C5)
  • western blot; human; loading ...; fig 6a
Abcam Mitf antibody (Abcam, ab12039) was used in western blot on human samples (fig 6a). Nat Commun (2021) ncbi
mouse monoclonal (D5)
  • immunohistochemistry; human; loading ...; fig 2a
Abcam Mitf antibody (abcam, ab3201) was used in immunohistochemistry on human samples (fig 2a). Cell Stem Cell (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; 1:100; loading ...; fig 4a
Abcam Mitf antibody (Abcam, AB122982) was used in immunohistochemistry on rat samples at 1:100 (fig 4a). Exp Mol Med (2021) ncbi
mouse monoclonal (C5)
  • western blot knockout validation; human; 1:2000; loading ...; fig s1-1a
Abcam Mitf antibody (Abcam, ab12039) was used in western blot knockout validation on human samples at 1:2000 (fig s1-1a). elife (2021) ncbi
mouse monoclonal (C5)
  • other; human; loading ...; fig 4c
Abcam Mitf antibody (Abcam, ab12039) was used in other on human samples (fig 4c). PLoS ONE (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:50; loading ...; fig 2f
Abcam Mitf antibody (Santa, ab20663) was used in immunocytochemistry on mouse samples at 1:50 (fig 2f). EBioMedicine (2020) ncbi
mouse monoclonal (C5)
  • immunohistochemistry; mouse; 1:400; loading ...; fig s1e
Abcam Mitf antibody (Abcam, ab12039) was used in immunohistochemistry on mouse samples at 1:400 (fig s1e). Nature (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4h
Abcam Mitf antibody (Abcam, ab20663) was used in western blot on human samples (fig 4h). Aging (Albany NY) (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3b
Abcam Mitf antibody (Abcam, ab20663) was used in western blot on mouse samples at 1:1000 (fig 3b). Mol Med Rep (2019) ncbi
domestic rabbit monoclonal (EPR9731)
  • western blot; human; 1:200; loading ...; fig 1a
Abcam Mitf antibody (Abcam, ab140606) was used in western blot on human samples at 1:200 (fig 1a). Cell Mol Biol Lett (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 3e
In order to characterize the differentiation of RPE cells, Abcam Mitf antibody (Abcam, ab20663) was used in immunocytochemistry on human samples (fig 3e). Stem Cell Res Ther (2017) ncbi
mouse monoclonal (C5)
  • western blot; mouse; 1:1000; loading ...; fig sf10d
Abcam Mitf antibody (Abcam, Ab12039) was used in western blot on mouse samples at 1:1000 (fig sf10d). J Clin Invest (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:500; fig 2A
In order to establish an application to generate retinal pigmented epithelium from induced pluripotent stem cells, Abcam Mitf antibody (Abcam, ab122982) was used in immunohistochemistry on human samples at 1:500 (fig 2A). PLoS ONE (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:50; fig 4h
In order to develop a novel method for obtaining perivascular-resident macrophage-like melanocytes, pericytes, and endothelial cells primary cells to study the vestibular blood-labyrinth barrier, Abcam Mitf antibody (Abcam, Ab20663) was used in immunocytochemistry on mouse samples at 1:50 (fig 4h). Hear Res (2017) ncbi
mouse monoclonal (C5)
  • western blot; human; fig 2a
In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Abcam Mitf antibody (Abcam, ab12039) was used in western blot on human samples (fig 2a). Cell Rep (2016) ncbi
mouse monoclonal (D5)
  • immunohistochemistry; human; loading ...; fig 3c
In order to investigate metastatic melanoma through single-cell RNA-seq, Abcam Mitf antibody (Abcam, ab3201) was used in immunohistochemistry on human samples (fig 3c). Science (2016) ncbi
mouse monoclonal (C5)
  • western blot; human; fig 2
In order to investigate regulation of the visual cycle genes Rdh5 and Rlbp1 in the retinal pigment epithelium by microphthalmia-associated transcription factor, Abcam Mitf antibody (Abcam, 12039) was used in western blot on human samples (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (C5)
  • western blot; pigs ; 1:1000; fig 5d
Abcam Mitf antibody (Abcam, ab12039) was used in western blot on pigs samples at 1:1000 (fig 5d). Sci Rep (2015) ncbi
mouse monoclonal (C5)
  • western blot; mouse; 1:1000; fig 6
Abcam Mitf antibody (Abcam, Ab12039) was used in western blot on mouse samples at 1:1000 (fig 6). PLoS ONE (2015) ncbi
mouse monoclonal (C5)
  • ChIP-Seq; human; fig 4
  • western blot; human; 1:1000; fig s7
Abcam Mitf antibody (Abcam, ab12039) was used in ChIP-Seq on human samples (fig 4) and in western blot on human samples at 1:1000 (fig s7). Nat Commun (2015) ncbi
mouse monoclonal (C5)
  • western blot; human
In order to study the promotion of c-Myc degradation by BLM helicase and its effect on tumor initiation, Abcam Mitf antibody (Abcam, ab12039) was used in western blot on human samples . J Cell Sci (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C5)
  • immunocytochemistry; human; loading ...; fig 1a
Santa Cruz Biotechnology Mitf antibody (Santa Cruz Biotechnology, sc-56725) was used in immunocytochemistry on human samples (fig 1a). Mol Ther Methods Clin Dev (2021) ncbi
mouse monoclonal (C5)
  • chromatin immunoprecipitation; human; loading ...; fig 5e
  • immunocytochemistry; human; loading ...; fig 4g
  • western blot; human; loading ...; fig 4e
Santa Cruz Biotechnology Mitf antibody (Santa Cruz, sc-56725) was used in chromatin immunoprecipitation on human samples (fig 5e), in immunocytochemistry on human samples (fig 4g) and in western blot on human samples (fig 4e). Mol Carcinog (2016) ncbi
mouse monoclonal (C5)
  • immunocytochemistry; mouse; 1:100; fig s1
In order to elucidate the relationship between MITF and c-Jun in melanoma, Santa Cruz Biotechnology Mitf antibody (Santa Cruz, sc-56725) was used in immunocytochemistry on mouse samples at 1:100 (fig s1). Nat Commun (2015) ncbi
mouse monoclonal (4H205)
  • chromatin immunoprecipitation; human
  • immunocytochemistry; human; fig 2a
  • western blot; human; fig 2c
Santa Cruz Biotechnology Mitf antibody (santa cruz, sc-71587) was used in chromatin immunoprecipitation on human samples , in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 2c). Nature (2015) ncbi
Articles Reviewed
  1. Hamm M, Sohier P, Petit V, Raymond J, Delmas V, Le Coz M, et al. BRN2 is a non-canonical melanoma tumor-suppressor. Nat Commun. 2021;12:3707 pubmed publisher
  2. Eriksen A, Møller R, Makovoz B, Uhl S, tenOever B, Blenkinsop T. SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium. Cell Stem Cell. 2021;28:1205-1220.e7 pubmed publisher
  3. Yang J, Chung S, Yun K, Kim B, So S, Kang S, et al. Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats. Exp Mol Med. 2021;53:631-642 pubmed publisher
  4. Salas A, Duarri A, Fontrodona L, Ram xed rez D, Badia A, Isla Magran xe9 H, et al. Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration. Mol Ther Methods Clin Dev. 2021;20:688-702 pubmed publisher
  5. Dilshat R, Fock V, Kenny C, Gerritsen I, Lasseur R, Travnickova J, et al. MITF reprograms the extracellular matrix and focal adhesion in melanoma. elife. 2021;10: pubmed publisher
  6. Ballesteros Álvarez J, Dilshat R, Fock V, Möller K, Karl L, Larue L, et al. MITF and TFEB cross-regulation in melanoma cells. PLoS ONE. 2020;15:e0238546 pubmed publisher
  7. Chen F, Liu X, Chen Y, Liu J, Lu H, Wang W, et al. Sphere-induced reprogramming of RPE cells into dual-potential RPE stem-like cells. EBioMedicine. 2020;52:102618 pubmed publisher
  8. Zhang B, Ma S, Rachmin I, He M, Baral P, Choi S, et al. Hyperactivation of sympathetic nerves drives depletion of melanocyte stem cells. Nature. 2020;577:676-681 pubmed publisher
  9. Tang L, Li J, Fu W, Wu W, Xu J. Suppression of FADS1 induces ROS generation, cell cycle arrest, and apoptosis in melanocytes: implications for vitiligo. Aging (Albany NY). 2019;11:11829-11843 pubmed publisher
  10. Qi S, Liu B, Zhang J, Liu X, Dong C, Fan R. Knockdown of microRNA‑143‑5p by STTM technology affects eumelanin and pheomelanin production in melanocytes. Mol Med Rep. 2019;20:2649-2656 pubmed publisher
  11. Pan H, Alamri A, Valapala M. Nutrient deprivation and lysosomal stress induce activation of TFEB in retinal pigment epithelial cells. Cell Mol Biol Lett. 2019;24:33 pubmed publisher
  12. Hazim R, Karumbayaram S, Jiang M, Dimashkie A, Lopes V, Li D, et al. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017;8:217 pubmed publisher
  13. Olvedy M, Tisserand J, Luciani F, Boeckx B, Wouters J, Lopez S, et al. Comparative oncogenomics identifies tyrosine kinase FES as a tumor suppressor in melanoma. J Clin Invest. 2017;127:2310-2325 pubmed publisher
  14. Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  15. Zhang J, Chen S, Cai J, Hou Z, Wang X, Kachelmeier A, et al. Culture media-based selection of endothelial cells, pericytes, and perivascular-resident macrophage-like melanocytes from the young mouse vestibular system. Hear Res. 2017;345:10-22 pubmed publisher
  16. Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
  17. Tirosh I, Izar B, Prakadan S, Wadsworth M, Treacy D, Trombetta J, et al. Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq. Science. 2016;352:189-96 pubmed publisher
  18. Wen B, Li S, Li H, Chen Y, Ma X, Wang J, et al. Microphthalmia-associated transcription factor regulates the visual cycle genes Rlbp1 and Rdh5 in the retinal pigment epithelium. Sci Rep. 2016;6:21208 pubmed publisher
  19. De Luca T, Pelosi A, Trisciuoglio D, D Aguanno S, Desideri M, Farini V, et al. miR-211 and MITF modulation by Bcl-2 protein in melanoma cells. Mol Carcinog. 2016;55:2304-2312 pubmed publisher
  20. Riesenberg S, Groetchen A, Siddaway R, Bald T, Reinhardt J, Smorra D, et al. MITF and c-Jun antagonism interconnects melanoma dedifferentiation with pro-inflammatory cytokine responsiveness and myeloid cell recruitment. Nat Commun. 2015;6:8755 pubmed publisher
  21. Wang X, Zhou J, Cao C, Huang J, Hai T, Wang Y, et al. Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Sci Rep. 2015;5:13348 pubmed publisher
  22. Perera R, Stoykova S, Nicolay B, Ross K, Fitamant J, Boukhali M, et al. Transcriptional control of autophagy-lysosome function drives pancreatic cancer metabolism. Nature. 2015;524:361-5 pubmed publisher
  23. Stemig M, Astelford K, Emery A, Cho J, Allen B, Huang T, et al. Deletion of histone deacetylase 7 in osteoclasts decreases bone mass in mice by interactions with MITF. PLoS ONE. 2015;10:e0123843 pubmed publisher
  24. Verfaillie A, Imrichová H, Atak Z, Dewaele M, Rambow F, Hulselmans G, et al. Decoding the regulatory landscape of melanoma reveals TEADS as regulators of the invasive cell state. Nat Commun. 2015;6:6683 pubmed publisher
  25. Chandra S, Priyadarshini R, Madhavan V, Tikoo S, Hussain M, Mudgal R, et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci. 2013;126:3782-95 pubmed publisher