This is a Validated Antibody Database (VAD) review about mouse Manf, based on 5 published articles (read how Labome selects the articles), using Manf antibody in all methods. It is aimed to help Labome visitors find the most suited Manf antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Manf synonym: 3230402M22Rik; AA407711; AA408789; AA673178; Armet; D17914; D18Mgi17; mesencephalic astrocyte-derived neurotrophic factor; arginine-rich protein; arginine-rich, mutated in early stage tumors

Abcam
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 1
In order to report the mechanism by which thrombospondin-4 regulates skeletal muscle integrity, Abcam Manf antibody (Abcam, ab67271) was used in western blot on mouse samples at 1:1000 (fig 1). elife (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3g
  • western blot; rat; fig 3e
In order to test if thrombospondin-4 is induced in association with ATF6alpha activation and endoplasmic reticulum expansion, Abcam Manf antibody (abcam, ab67271) was used in western blot on mouse samples (fig 3g) and in western blot on rat samples (fig 3e). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:500; fig 3
Abcam Manf antibody (abcam, ab67271) was used in western blot on rat samples at 1:500 (fig 3). PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
In order to identify proteins that interact with RTN1-C, Abcam Manf antibody (Abcam, ab67271) was used in immunoprecipitation on human samples , in immunocytochemistry on human samples and in western blot on human samples . Acta Biochim Biophys Sin (Shanghai) (2015) ncbi
ProSci
rabbit polyclonal
  • immunohistochemistry; zebrafish ; 1:3000
  • western blot; zebrafish ; 1:2000
In order to study the role of MANF during dopaminergic neuron development in larval zebrafish, ProSci Manf antibody (ProSci Inc, 4347) was used in immunohistochemistry on zebrafish samples at 1:3000 and in western blot on zebrafish samples at 1:2000. Dev Biol (2012) ncbi
Articles Reviewed
  1. Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
  2. Krawczyk K, Ekman M, Rippe C, Grossi M, Nilsson B, Albinsson S, et al. Assessing the contribution of thrombospondin-4 induction and ATF6? activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction. Sci Rep. 2016;6:32449 pubmed publisher
  3. Palao T, Swärd K, Jongejan A, Moerland P, de Vos J, van Weert A, et al. Gene Expression and MicroRNA Expression Analysis in Small Arteries of Spontaneously Hypertensive Rats. Evidence for ER Stress. PLoS ONE. 2015;10:e0137027 pubmed publisher
  4. Chen L, Wan L, Du J, Shen Y. Identification of MANF as a protein interacting with RTN1-C. Acta Biochim Biophys Sin (Shanghai). 2015;47:91-7 pubmed publisher
  5. Chen Y, Sundvik M, Rozov S, Priyadarshini M, Panula P. MANF regulates dopaminergic neuron development in larval zebrafish. Dev Biol. 2012;370:237-49 pubmed publisher