This is a Validated Antibody Database (VAD) review about mouse MDA5, based on 25 published articles (read how Labome selects the articles), using MDA5 antibody in all methods. It is aimed to help Labome visitors find the most suited MDA5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MDA5 synonym: 9130009C22Rik; Helicard; Hlcd; MDA5; RLR-2

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (D74E4)
  • western blot knockout validation; human; loading ...; fig 3a
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot knockout validation on human samples (fig 3a). iScience (2022) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D74E4)
  • western blot knockout validation; human; 1:500; loading ...; fig 4a
Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, 5321) was used in western blot knockout validation on human samples at 1:500 (fig 4a). JCI Insight (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D74E4)
  • western blot knockout validation; mouse; loading ...; fig s8f
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot knockout validation on mouse samples (fig s8f). Hepatology (2018) ncbi
Invitrogen
domestic rabbit recombinant (33H12L34)
  • immunocytochemistry; human; loading ...; fig 10g
In order to report that rabies virus induces stress granule formation, Invitrogen MDA5 antibody (Invitrogen, 33H12L34) was used in immunocytochemistry on human samples (fig 10g). PLoS Pathog (2016) ncbi
domestic rabbit recombinant (33H12L34)
  • western blot; human
In order to study the IFN-lambda inate immune response of HepG2 cells in response to HCV infection, Invitrogen MDA5 antibody (Invitrogen, 33H12L34) was used in western blot on human samples . Hepatology (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D74E4)
  • western blot knockout validation; human; loading ...; fig 3a
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot knockout validation on human samples (fig 3a). iScience (2022) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; 1:1000; loading ...; fig 3a
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples at 1:1000 (fig 3a). Proc Natl Acad Sci U S A (2022) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; 1:1000; loading ...; fig 9b
Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, 5321) was used in western blot on human samples at 1:1000 (fig 9b). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; 1:1000; loading ...
Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, 5321) was used in western blot on human samples at 1:1000. Signal Transduct Target Ther (2021) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot knockout validation; human; 1:500; loading ...; fig 4a
Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, 5321) was used in western blot knockout validation on human samples at 1:500 (fig 4a). JCI Insight (2020) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; mouse; loading ...
Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, D74E4) was used in western blot on mouse samples . elife (2019) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; 1:3000; loading ...
Cell Signaling Technology MDA5 antibody (CST, 5321) was used in western blot on human samples at 1:3000. elife (2019) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; mouse; loading ...; fig 7e
Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, D74E4) was used in western blot on mouse samples (fig 7e). Nature (2019) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 3f). PLoS ONE (2018) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; fig s7b
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig s7b). Cell (2018) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; fig 7e
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 7e). Cell (2018) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot knockout validation; mouse; loading ...; fig s8f
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot knockout validation on mouse samples (fig s8f). Hepatology (2018) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; mouse; 1:2000; loading ...; fig s4f
In order to describe the mechanism by which FBXW7 stabilizes RigI, Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on mouse samples at 1:2000 (fig s4f). Nat Commun (2017) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on mouse samples at 1:1000 (fig 4b). Nucleic Acids Res (2017) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; loading ...; fig 7d
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 7d). J Neuroinflammation (2016) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; fig 6
In order to assess the effect of polyinosinic-polycytidylic acid on the barrier function and tight junction integrity of primary human lung microvascular endothelial cells, Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 6). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; fig 1
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 1). Sci Rep (2016) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; loading ...; fig 1e
In order to identify the viral RNA signature on RIG-I-like receptors during viral infection, Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 1e). elife (2016) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology MDA5 antibody (Cell Signaling, D74E4) was used in western blot on human samples at 1:1000 (fig 5). PLoS Pathog (2016) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; pigs ; loading ...; fig 10a
Cell Signaling Technology MDA5 antibody (Cell Signaling, D74E4) was used in western blot on pigs samples (fig 10a). MBio (2015) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; mouse; fig 1
In order to analyze the antiviral innate immune response due to mitochondrial DNA stress, Cell Signaling Technology MDA5 antibody (Cell Signaling Technology, D74E4) was used in western blot on mouse samples (fig 1). Nature (2015) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; human; fig 6a
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on human samples (fig 6a). Mol Cancer (2014) ncbi
domestic rabbit monoclonal (D74E4)
  • western blot; mouse
Cell Signaling Technology MDA5 antibody (Cell Signaling, 5321) was used in western blot on mouse samples . PLoS Pathog (2014) ncbi
Articles Reviewed
  1. Tan H, Yong Y, Xue Y, Liu H, Furihata T, Shankar E, et al. cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model. iScience. 2022;25:104404 pubmed publisher
  2. Wolpaw A, Grossmann L, Dessau J, Dong M, Aaron B, Brafford P, et al. Epigenetic state determines inflammatory sensing in neuroblastoma. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
  3. Lee C, Kerouanton B, Chothani S, Zhang S, Chen Y, Mantri C, et al. Coding and non-coding roles of MOCCI (C15ORF48) coordinate to regulate host inflammation and immunity. Nat Commun. 2021;12:2130 pubmed publisher
  4. Guo G, Gao M, Gao X, Zhu B, Huang J, Luo K, et al. SARS-CoV-2 non-structural protein 13 (nsp13) hijacks host deubiquitinase USP13 and counteracts host antiviral immune response. Signal Transduct Target Ther. 2021;6:119 pubmed publisher
  5. de Cubas A, Dunker W, Zaninovich A, Hongo R, Bhatia A, Panda A, et al. DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer. JCI Insight. 2020;5: pubmed publisher
  6. Witteveldt J, Knol L, Macias S. MicroRNA-deficient mouse embryonic stem cells acquire a functional interferon response. elife. 2019;8: pubmed publisher
  7. Xu H, Xu S, Xie S, Zhang Y, Yang J, Zhang W, et al. MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway. elife. 2019;8: pubmed publisher
  8. Ishizuka J, Manguso R, Cheruiyot C, Bi K, Panda A, Iracheta Vellve A, et al. Loss of ADAR1 in tumours overcomes resistance to immune checkpoint blockade. Nature. 2019;565:43-48 pubmed publisher
  9. Ghosh R, Roy S, Franco S. PARP1 depletion induces RIG-I-dependent signaling in human cancer cells. PLoS ONE. 2018;13:e0194611 pubmed publisher
  10. Ahmad S, Mu X, Yang F, Greenwald E, Park J, Jacob E, et al. Breaching Self-Tolerance to Alu Duplex RNA Underlies MDA5-Mediated Inflammation. Cell. 2018;172:797-810.e13 pubmed publisher
  11. Chung H, Calis J, Wu X, Sun T, Yu Y, Sarbanes S, et al. Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. Cell. 2018;172:811-824.e14 pubmed publisher
  12. Wang W, Wang Y, Qu C, Wang S, Zhou J, Cao W, et al. The RNA genome of hepatitis E virus robustly triggers an antiviral interferon response. Hepatology. 2018;67:2096-2112 pubmed publisher
  13. Song Y, Lai L, Chong Z, He J, Zhang Y, Xue Y, et al. E3 ligase FBXW7 is critical for RIG-I stabilization during antiviral responses. Nat Commun. 2017;8:14654 pubmed publisher
  14. Nikolic J, Civas A, Lama Z, Lagaudrière Gesbert C, Blondel D. Rabies Virus Infection Induces the Formation of Stress Granules Closely Connected to the Viral Factories. PLoS Pathog. 2016;12:e1005942 pubmed publisher
  15. Pépin G, Nejad C, Thomas B, Ferrand J, McArthur K, Bardin P, et al. Activation of cGAS-dependent antiviral responses by DNA intercalating agents. Nucleic Acids Res. 2017;45:198-205 pubmed publisher
  16. Zhang X, Zheng Z, Liu X, Shu B, Mao P, Bai B, et al. Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway. J Neuroinflammation. 2016;13:209 pubmed publisher
  17. Huang L, Stuart C, Takeda K, D Agnillo F, Golding B. Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5. PLoS ONE. 2016;11:e0160875 pubmed publisher
  18. Hou Z, Zhang J, Han Q, Su C, Qu J, Xu D, et al. Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a. Sci Rep. 2016;6:26150 pubmed publisher
  19. Sanchez David R, Combredet C, Sismeiro O, Dillies M, Jagla B, Coppée J, et al. Comparative analysis of viral RNA signatures on different RIG-I-like receptors. elife. 2016;5:e11275 pubmed publisher
  20. Pham A, Santa Maria F, Lahiri T, Friedman E, Marié I, Levy D. PKR Transduces MDA5-Dependent Signals for Type I IFN Induction. PLoS Pathog. 2016;12:e1005489 pubmed publisher
  21. Marquez Jurado S, Nogales A, Zuñiga S, Enjuanes L, Almazán F. Identification of a gamma interferon-activated inhibitor of translation-like RNA motif at the 3' end of the transmissible gastroenteritis coronavirus genome modulating innate immune response. MBio. 2015;6:e00105 pubmed publisher
  22. West A, Khoury Hanold W, Staron M, Tal M, Pineda C, Lang S, et al. Mitochondrial DNA stress primes the antiviral innate immune response. Nature. 2015;520:553-7 pubmed publisher
  23. Harashima N, Minami T, Uemura H, Harada M. Transfection of poly(I:C) can induce reactive oxygen species-triggered apoptosis and interferon-β-mediated growth arrest in human renal cell carcinoma cells via innate adjuvant receptors and the 2-5A system. Mol Cancer. 2014;13:217 pubmed publisher
  24. Israelow B, Narbus C, Sourisseau M, Evans M. HepG2 cells mount an effective antiviral interferon-lambda based innate immune response to hepatitis C virus infection. Hepatology. 2014;60:1170-9 pubmed publisher
  25. Huang Y, Liu H, Li S, Tang Y, Wei B, Yu H, et al. MAVS-MKK7-JNK2 defines a novel apoptotic signaling pathway during viral infection. PLoS Pathog. 2014;10:e1004020 pubmed publisher