This is a Validated Antibody Database (VAD) review about mouse MD 2, based on 12 published articles (read how Labome selects the articles), using MD 2 antibody in all methods. It is aimed to help Labome visitors find the most suited MD 2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MD 2 synonym: ESOP-1; MD-2; MD2; lymphocyte antigen 96; ly-96; myeloid differentiation factor-2; myeloid differentiation protein-2; protein MD-2

Invitrogen
rat monoclonal (MTS510)
  • flow cytometry; mouse; loading ...
In order to show that TLR4 signals through the BCR leading to activation of SYK, ERK, and AKT as well as through MYD88 leading to activation of NFkappaB, Invitrogen MD 2 antibody (eBioscience, MTS510) was used in flow cytometry on mouse samples . J Exp Med (2017) ncbi
rat monoclonal (MTS510)
  • blocking or activating experiments; human; loading ...; fig 4c
In order to implicate myeloid differentiation factor-2 as the high-affinity soluble CD83 binding partner, Invitrogen MD 2 antibody (eBioscience, MTS510) was used in blocking or activating experiments on human samples (fig 4c). J Immunol (2017) ncbi
rat monoclonal (MTS510)
  • flow cytometry; mouse; fig 6d
In order to discover structural elements that distinguish Grp94 from Hsp90, Invitrogen MD 2 antibody (eBioscience, MTS510) was used in flow cytometry on mouse samples (fig 6d). PLoS ONE (2016) ncbi
rat monoclonal (MTS510)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5a
In order to investigate the role of extracellular vesicles by the choroid plexus epithelium in blood-brain communication, Invitrogen MD 2 antibody (eBioscience, 13-9924-81) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 5a). EMBO Mol Med (2016) ncbi
rat monoclonal (MTS510)
  • flow cytometry; mouse
Invitrogen MD 2 antibody (eBioscience, MTS510) was used in flow cytometry on mouse samples . J Biol Chem (2013) ncbi
rat monoclonal (MTS510)
  • flow cytometry; mouse
Invitrogen MD 2 antibody (eBioscience, MTS510) was used in flow cytometry on mouse samples . J Biol Chem (2012) ncbi
rat monoclonal (MTS510)
  • flow cytometry; mouse
In order to report that SLAM is a microbial sensor aids in the killing of gram-negative bacteria by macrophages, Invitrogen MD 2 antibody (eBioscience, MTS510) was used in flow cytometry on mouse samples . Nat Immunol (2010) ncbi
rat monoclonal (MTS510)
  • immunohistochemistry; rat; 1:100; fig 2
In order to use rats to test if changes in gut epithelial function and microbiota are diet- or obesity-associated, Invitrogen MD 2 antibody (eBioscience, 14-9924) was used in immunohistochemistry on rat samples at 1:100 (fig 2). Am J Physiol Gastrointest Liver Physiol (2010) ncbi
rat monoclonal (MTS510)
  • flow cytometry; mouse; fig 3
In order to characterize and compare T helper 1 and 17 cells, Invitrogen MD 2 antibody (eBiosciences, MTS510) was used in flow cytometry on mouse samples (fig 3). J Leukoc Biol (2007) ncbi
rat monoclonal (MTS510)
  • flow cytometry; mouse
In order to study TLR2 and 4 expression in a trinitrobenzene sulfonic acid-induced colitis model of Crohn's disease, Invitrogen MD 2 antibody (eBioscience, MTS510) was used in flow cytometry on mouse samples . J Leukoc Biol (2005) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; mouse; loading ...; fig 8a
Novus Biologicals MD 2 antibody (Novus Biologicals, NB100-56655) was used in western blot on mouse samples (fig 8a). Sci Rep (2017) ncbi
Santa Cruz Biotechnology
rat monoclonal (MTS510)
  • flow cytometry; mouse
Santa Cruz Biotechnology MD 2 antibody (Santa Cruz Biotechnology, sc-13591) was used in flow cytometry on mouse samples . Exp Mol Med (2014) ncbi
Articles Reviewed
  1. Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
  2. Horvatinovich J, Grogan E, Norris M, Steinkasserer A, Lemos H, Mellor A, et al. Soluble CD83 Inhibits T Cell Activation by Binding to the TLR4/MD-2 Complex on CD14+ Monocytes. J Immunol. 2017;198:2286-2301 pubmed publisher
  3. Xiong X, Liu Y, Mei Y, Peng J, Wang Z, Kong B, et al. Novel Protective Role of Myeloid Differentiation 1 in Pathological Cardiac Remodelling. Sci Rep. 2017;7:41857 pubmed publisher
  4. Maharaj K, Que N, Hong F, Huck J, Gill S, Wu S, et al. Exploring the Functional Complementation between Grp94 and Hsp90. PLoS ONE. 2016;11:e0166271 pubmed publisher
  5. Balusu S, Van Wonterghem E, De Rycke R, Raemdonck K, Stremersch S, Gevaert K, et al. Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles. EMBO Mol Med. 2016;8:1162-1183 pubmed publisher
  6. Jin Y, Wi H, Choi M, Hong S, Bae Y. Regulation of anti-inflammatory cytokines IL-10 and TGF-? in mouse dendritic cells through treatment with Clonorchis sinensis crude antigen. Exp Mol Med. 2014;46:e74 pubmed publisher
  7. Hong F, Liu B, Chiosis G, Gewirth D, Li Z. ?7 helix region of ?I domain is crucial for integrin binding to endoplasmic reticulum chaperone gp96: a potential therapeutic target for cancer metastasis. J Biol Chem. 2013;288:18243-8 pubmed publisher
  8. Wu S, Hong F, Gewirth D, Guo B, Liu B, Li Z. The molecular chaperone gp96/GRP94 interacts with Toll-like receptors and integrins via its C-terminal hydrophobic domain. J Biol Chem. 2012;287:6735-42 pubmed publisher
  9. Berger S, Romero X, Ma C, Wang G, Faubion W, Liao G, et al. SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages. Nat Immunol. 2010;11:920-7 pubmed publisher
  10. De la Serre C, Ellis C, Lee J, Hartman A, Rutledge J, Raybould H. Propensity to high-fat diet-induced obesity in rats is associated with changes in the gut microbiota and gut inflammation. Am J Physiol Gastrointest Liver Physiol. 2010;299:G440-8 pubmed publisher
  11. Nakae S, Iwakura Y, Suto H, Galli S. Phenotypic differences between Th1 and Th17 cells and negative regulation of Th1 cell differentiation by IL-17. J Leukoc Biol. 2007;81:1258-68 pubmed
  12. Gomariz R, Arranz A, Abad C, Torroba M, Martinez C, Rosignoli F, et al. Time-course expression of Toll-like receptors 2 and 4 in inflammatory bowel disease and homeostatic effect of VIP. J Leukoc Biol. 2005;78:491-502 pubmed