This is a Validated Antibody Database (VAD) review about mouse Lrrk2, based on 27 published articles (read how Labome selects the articles), using Lrrk2 antibody in all methods. It is aimed to help Labome visitors find the most suited Lrrk2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Lrrk2 synonym: 4921513O20Rik; 9330188B09Rik; AW561911; D630001M17Rik; Gm927; cI-46

Knockout validation
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s7b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot knockout validation on mouse samples at 1:1000 (fig s7b). Acta Neuropathol (2019) ncbi
Abcam
domestic rabbit monoclonal (UDD2 10(12))
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s1a
Abcam Lrrk2 antibody (Abcam, ab133450) was used in western blot knockout validation on mouse samples at 1:2000 (fig s1a). NPJ Parkinsons Dis (2018) ncbi
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s1a
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot knockout validation on mouse samples at 1:2000 (fig s1a). NPJ Parkinsons Dis (2018) ncbi
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; human; loading ...; fig 5b
  • immunoprecipitation; human; loading ...; fig 6e
In order to find that LRRK2 regulates Rip2 and promotes inflammatory cytokine induction through the Nod1/2-Rip2 pathway, Abcam Lrrk2 antibody (Epitomics, MJFF2 c41-2) was used in western blot knockout validation on human samples (fig 5b) and in immunoprecipitation on human samples (fig 6e). Protein Cell (2017) ncbi
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; fig 7
Abcam Lrrk2 antibody (Epitomics, MJFF2) was used in western blot knockout validation on mouse samples at 1:2000 (fig 7). J Biol Chem (2014) ncbi
Abcam
domestic rabbit monoclonal (UDD3 30(12))
  • western blot knockout validation; mouse; fig 1
  • immunoprecipitation; mouse; fig 2
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
Abcam Lrrk2 antibody (Abcam, ab133518) was used in western blot knockout validation on mouse samples (fig 1), in immunoprecipitation on mouse samples (fig 2), in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 1). Biochem J (2013) ncbi
Abcam
domestic rabbit monoclonal (MJFF4)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig 1
  • immunoprecipitation; mouse; fig 2
Abcam Lrrk2 antibody (Abcam, ab181386) was used in immunoprecipitation on human samples (fig 2), in western blot on human samples (fig 1), in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot knockout validation on mouse samples (fig 1) and in immunoprecipitation on mouse samples (fig 2). Biochem J (2013) ncbi
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; rat; fig 6
  • immunohistochemistry - free floating section; rat; fig 6
  • western blot; human; fig 1
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig 1, s2
  • immunohistochemistry - free floating section; mouse; fig 4
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunohistochemistry knockout validation on rat samples (fig 6), in immunohistochemistry - free floating section on rat samples (fig 6), in western blot on human samples (fig 1), in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot knockout validation on mouse samples (fig 1, s2) and in immunohistochemistry - free floating section on mouse samples (fig 4). Biochem J (2013) ncbi
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; mouse; 1:4000; fig 1
Abcam Lrrk2 antibody (Epitomics, MJFF2 (c41-2)) was used in immunohistochemistry knockout validation on mouse samples at 1:4000 (fig 1). Mol Neurodegener (2012) ncbi
Abcam
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; mouse; 1:300; loading ...; fig s5b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on mouse samples at 1:300 (fig s5b). Neurobiol Dis (2021) ncbi
domestic rabbit monoclonal (UDD2 10(12))
  • western blot; human; loading ...; fig s1
Abcam Lrrk2 antibody (Abcam, ab133450) was used in western blot on human samples (fig s1). Clin Transl Med (2021) ncbi
domestic rabbit monoclonal (UDD3 30(12))
  • western blot; human; loading ...; fig 2a
Abcam Lrrk2 antibody (Abcam, ab133518) was used in western blot on human samples (fig 2a). J Cell Biol (2019) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s7b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot knockout validation on mouse samples at 1:1000 (fig s7b). Acta Neuropathol (2019) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s1a
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot knockout validation on mouse samples at 1:2000 (fig s1a). NPJ Parkinsons Dis (2018) ncbi
domestic rabbit monoclonal (UDD2 10(12))
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s1a
Abcam Lrrk2 antibody (Abcam, ab133450) was used in western blot knockout validation on mouse samples at 1:2000 (fig s1a). NPJ Parkinsons Dis (2018) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3e
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunohistochemistry - frozen section on mouse samples (fig s3e). Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit monoclonal
  • western blot; human; loading ...; fig 8a
Abcam Lrrk2 antibody (Abcam, ab169521) was used in western blot on human samples (fig 8a). EMBO J (2018) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; mouse; 1:300; loading ...; fig 1
In order to elucidate the effects of mutations in leucine-rich repeat kinase 2 on nigro0striatal dopamine neurons, Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on mouse samples at 1:300 (fig 1). Acta Neuropathol Commun (2017) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; human; loading ...; fig 5b
  • immunoprecipitation; human; loading ...; fig 6e
In order to find that LRRK2 regulates Rip2 and promotes inflammatory cytokine induction through the Nod1/2-Rip2 pathway, Abcam Lrrk2 antibody (Epitomics, MJFF2 c41-2) was used in western blot knockout validation on human samples (fig 5b) and in immunoprecipitation on human samples (fig 6e). Protein Cell (2017) ncbi
domestic rabbit monoclonal (UDD2 10(12))
  • western blot; human; fig s4
Abcam Lrrk2 antibody (Abcam, ab133450) was used in western blot on human samples (fig s4). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; fig 2b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples (fig 2b). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (MJFF3 (c69-6))
  • immunohistochemistry; zebrafish ; 1:200; loading ...; fig 6a
  • western blot; zebrafish ; 1:500; loading ...; fig 1a
In order to describe multifaceted roles of LRRK2 in zebrafish, Abcam Lrrk2 antibody (Abcam, ab133475) was used in immunohistochemistry on zebrafish samples at 1:200 (fig 6a) and in western blot on zebrafish samples at 1:500 (fig 1a). J Neurosci Res (2016) ncbi
domestic rabbit monoclonal (UDD2 10(12))
  • immunocytochemistry; human
  • western blot; human; fig s3
In order to study the effect of G2019S on neuronal subtypes, Abcam Lrrk2 antibody (Abcam, ab133450) was used in immunocytochemistry on human samples and in western blot on human samples (fig s3). Stem Cell Reports (2015) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; 1:2000; loading ...; fig 1c
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples at 1:2000 (fig 1c). Mol Neurobiol (2016) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • immunoprecipitation; human; fig 2a
  • western blot; human; fig 2b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 2b). Mol Brain (2015) ncbi
domestic rabbit monoclonal (UDD2 10(12))
  • western blot; mouse; 1:500; fig 6d
Abcam Lrrk2 antibody (Abcam, ab133450) was used in western blot on mouse samples at 1:500 (fig 6d). Mol Brain (2015) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; mouse; 1:1000; fig 4
  • western blot; rat; 1:1000; fig 3
  • western blot; human; 1:1000; fig 3
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on mouse samples at 1:1000 (fig 4), in western blot on rat samples at 1:1000 (fig 3) and in western blot on human samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
domestic rabbit monoclonal (UDD2 10(12))
  • immunoprecipitation; human; 1:1000; fig 8
Abcam Lrrk2 antibody (Abcam, ab133450) was used in immunoprecipitation on human samples at 1:1000 (fig 8). Nat Commun (2015) ncbi
domestic rabbit monoclonal (MJFF4)
  • immunoprecipitation; mouse; fig s3
  • immunoprecipitation; human; fig 3
  • immunoprecipitation; rat; fig 3
Abcam Lrrk2 antibody (Abcam, ab181386) was used in immunoprecipitation on mouse samples (fig s3), in immunoprecipitation on human samples (fig 3) and in immunoprecipitation on rat samples (fig 3). Nat Commun (2015) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; 1:3000; fig 1c
  • western blot; mouse; 1:3000; fig 1d
In order to analyze protein kinase LRRK2, parkinson's disease associated, which modulates notch signaling via the endosomal pathway, Abcam Lrrk2 antibody (Abcam, MJFF2) was used in western blot on human samples at 1:3000 (fig 1c) and in western blot on mouse samples at 1:3000 (fig 1d). PLoS Genet (2015) ncbi
domestic rabbit monoclonal (MJFF4)
  • western blot; mouse; 1:1000; fig 2d
In order to compare cognitive and motor behaviors in various LRRK2 transgenic mice, Abcam Lrrk2 antibody (Abcam, ab133476) was used in western blot on mouse samples at 1:1000 (fig 2d). Parkinsonism Relat Disord (2015) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human
In order to elucidate the relationship between LRRK2 and Rab5b, Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples . J Biochem (2015) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; 1:1000; fig 3
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples at 1:1000 (fig 3). Biomed Res Int (2014) ncbi
domestic rabbit monoclonal (MJFF4)
  • western blot; rat
In order to examine the p.G2019S mutation in the leucine-rich repeat kinase 2 (LRRK2) and it's role in Parkinson's disease, Abcam Lrrk2 antibody (Abcam, ab133476) was used in western blot on rat samples . J Parkinsons Dis (2014) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; fig 7
Abcam Lrrk2 antibody (Epitomics, MJFF2) was used in western blot knockout validation on mouse samples at 1:2000 (fig 7). J Biol Chem (2014) ncbi
domestic rabbit monoclonal (UDD3 30(12))
  • western blot knockout validation; mouse; fig 1
  • immunoprecipitation; mouse; fig 2
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
Abcam Lrrk2 antibody (Abcam, ab133518) was used in western blot knockout validation on mouse samples (fig 1), in immunoprecipitation on mouse samples (fig 2), in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 1). Biochem J (2013) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; rat; fig 6
  • immunohistochemistry - free floating section; rat; fig 6
  • western blot; human; fig 1
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig 1, s2
  • immunohistochemistry - free floating section; mouse; fig 4
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunohistochemistry knockout validation on rat samples (fig 6), in immunohistochemistry - free floating section on rat samples (fig 6), in western blot on human samples (fig 1), in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot knockout validation on mouse samples (fig 1, s2) and in immunohistochemistry - free floating section on mouse samples (fig 4). Biochem J (2013) ncbi
domestic rabbit monoclonal (MJFF4)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig 1
  • immunoprecipitation; mouse; fig 2
Abcam Lrrk2 antibody (Abcam, ab181386) was used in immunoprecipitation on human samples (fig 2), in western blot on human samples (fig 1), in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot knockout validation on mouse samples (fig 1) and in immunoprecipitation on mouse samples (fig 2). Biochem J (2013) ncbi
domestic rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; mouse; 1:4000; fig 1
Abcam Lrrk2 antibody (Epitomics, MJFF2 (c41-2)) was used in immunohistochemistry knockout validation on mouse samples at 1:4000 (fig 1). Mol Neurodegener (2012) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; fruit fly ; 1:5000; loading ...; fig 1
In order to find that knocking down the leucine-rich repeat kinase gene by RNAi in DAergic neurons untreated or treated with paraquat neither affects the number of DAergic clusters, tyrosine hydroxylase protein levels, lifespan nor locomotor activity, Invitrogen Lrrk2 antibody (Thermo, PA1-16770) was used in western blot on fruit fly samples at 1:5000 (fig 1). Brain Res (2017) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 3c
In order to discuss the contribution of leucine-rich repeat kinase 2 mutations to Parkinson's disease, Novus Biologicals Lrrk2 antibody (Novus Biologicals, NB110-58771) was used in western blot on human samples at 1:1000 (fig 3c). J Neuroinflammation (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 4f
Cell Signaling Technology Lrrk2 antibody (Cell Signaling Technology, 5559) was used in western blot on rat samples (fig 4f). Am J Physiol Endocrinol Metab (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4d
In order to discuss the contribution of LINK-A to cancer, Cell Signaling Technology Lrrk2 antibody (Cell Signaling, 5559) was used in western blot on human samples at 1:1000 (fig 4d). Nat Cell Biol (2016) ncbi
Articles Reviewed
  1. Albanese F, Mercatelli D, Finetti L, Lamonaca G, Pizzi S, Shimshek D, et al. Constitutive silencing of LRRK2 kinase activity leads to early glucocerebrosidase deregulation and late impairment of autophagy in vivo. Neurobiol Dis. 2021;159:105487 pubmed publisher
  2. Chen L, Hou J, Zeng X, Guo Q, Deng M, Kloeber J, et al. LRRK2 inhibition potentiates PARP inhibitor cytotoxicity through inhibiting homologous recombination-mediated DNA double strand break repair. Clin Transl Med. 2021;11:e341 pubmed publisher
  3. Gomez R, Wawro P, Lis P, Alessi D, Pfeffer S. Membrane association but not identity is required for LRRK2 activation and phosphorylation of Rab GTPases. J Cell Biol. 2019;218:4157-4170 pubmed publisher
  4. Bieri G, Brahic M, Bousset L, Couthouis J, Kramer N, Ma R, et al. LRRK2 modifies α-syn pathology and spread in mouse models and human neurons. Acta Neuropathol. 2019;137:961-980 pubmed publisher
  5. Kluss J, Conti M, Kaganovich A, Beilina A, Melrose H, Cookson M, et al. Detection of endogenous S1292 LRRK2 autophosphorylation in mouse tissue as a readout for kinase activity. NPJ Parkinsons Dis. 2018;4:13 pubmed publisher
  6. Xiong Y, Neifert S, Karuppagounder S, Liu Q, Stankowski J, Lee B, et al. Robust kinase- and age-dependent dopaminergic and norepinephrine neurodegeneration in LRRK2 G2019S transgenic mice. Proc Natl Acad Sci U S A. 2018;115:1635-1640 pubmed publisher
  7. Purlyte E, Dhekne H, Sarhan A, Gomez R, Lis P, Wightman M, et al. Rab29 activation of the Parkinson's disease-associated LRRK2 kinase. EMBO J. 2018;37:1-18 pubmed publisher
  8. Pinto S, Lamon S, Stephenson E, Kalanon M, Mikovic J, Koch L, et al. Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity. Am J Physiol Endocrinol Metab. 2017;313:E335-E343 pubmed publisher
  9. Longo F, Mercatelli D, Novello S, Arcuri L, Brugnoli A, Vincenzi F, et al. Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice. Acta Neuropathol Commun. 2017;5:22 pubmed publisher
  10. Quintero Espinosa D, Jimenez Del Rio M, Velez Pardo C. Knockdown transgenic Lrrk Drosophila resists paraquat-induced locomotor impairment and neurodegeneration: A therapeutic strategy for Parkinson's disease. Brain Res. 2017;1657:253-261 pubmed publisher
  11. López de Maturana R, Lang V, Zubiarrain A, Sousa A, Vázquez N, Gorostidi A, et al. Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons. J Neuroinflammation. 2016;13:295 pubmed
  12. Yan R, Liu Z. LRRK2 enhances Nod1/2-mediated inflammatory cytokine production by promoting Rip2 phosphorylation. Protein Cell. 2017;8:55-66 pubmed publisher
  13. Speidel A, Felk S, Reinhardt P, Sterneckert J, Gillardon F. Leucine-Rich Repeat Kinase 2 Influences Fate Decision of Human Monocytes Differentiated from Induced Pluripotent Stem Cells. PLoS ONE. 2016;11:e0165949 pubmed publisher
  14. Prabhudesai S, Bensabeur F, Abdullah R, Basak I, Baez S, Alves G, et al. LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. J Neurosci Res. 2016;94:717-35 pubmed publisher
  15. Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1? signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed publisher
  16. Schwab A, Ebert A. Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation. Stem Cell Reports. 2015;5:1039-1052 pubmed publisher
  17. Smith G, Jansson J, Rocha E, Osborn T, Hallett P, Isacson O. Fibroblast Biomarkers of Sporadic Parkinson's Disease and LRRK2 Kinase Inhibition. Mol Neurobiol. 2016;53:5161-77 pubmed publisher
  18. Ho D, Kim H, Kim J, Sim H, Ahn H, Kim J, et al. Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21(WAF1/CIP1) expression. Mol Brain. 2015;8:54 pubmed publisher
  19. Choi I, Kim B, Byun J, Baik S, Huh Y, Kim J, et al. LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase. Nat Commun. 2015;6:8255 pubmed publisher
  20. Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
  21. Volta M, Cataldi S, Beccano Kelly D, Munsie L, Tatarnikov I, Chou P, et al. Chronic and acute LRRK2 silencing has no long-term behavioral effects, whereas wild-type and mutant LRRK2 overexpression induce motor and cognitive deficits and altered regulation of dopamine release. Parkinsonism Relat Disord. 2015;21:1156-63 pubmed publisher
  22. Yun H, Kim H, Ga I, Oh H, Ho D, Kim J, et al. An early endosome regulator, Rab5b, is an LRRK2 kinase substrate. J Biochem. 2015;157:485-95 pubmed publisher
  23. Ho D, Yi S, Seo H, Son I, Seol W. Increased DJ-1 in urine exosome of Korean males with Parkinson's disease. Biomed Res Int. 2014;2014:704678 pubmed publisher
  24. Walker M, Volta M, Cataldi S, Dinelle K, Beccano Kelly D, Munsie L, et al. Behavioral deficits and striatal DA signaling in LRRK2 p.G2019S transgenic rats: a multimodal investigation including PET neuroimaging. J Parkinsons Dis. 2014;4:483-98 pubmed publisher
  25. Law B, Spain V, Leinster V, Chia R, Beilina A, Cho H, et al. A direct interaction between leucine-rich repeat kinase 2 and specific ?-tubulin isoforms regulates tubulin acetylation. J Biol Chem. 2014;289:895-908 pubmed publisher
  26. Davies P, Hinkle K, Sukar N, Sepulveda B, Mesias R, Serrano G, et al. Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies. Biochem J. 2013;453:101-13 pubmed publisher
  27. Hinkle K, Yue M, Behrouz B, Dachsel J, Lincoln S, Bowles E, et al. LRRK2 knockout mice have an intact dopaminergic system but display alterations in exploratory and motor co-ordination behaviors. Mol Neurodegener. 2012;7:25 pubmed publisher