This is a Validated Antibody Database (VAD) review about mouse Lrig1, based on 8 published articles (read how Labome selects the articles), using Lrig1 antibody in all methods. It is aimed to help Labome visitors find the most suited Lrig1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Lrig1 synonym: D6Bwg0781e; Img; LIG-1; leucine-rich repeats and immunoglobulin-like domains protein 1; integral membrane glycoprotein

R&D Systems
goat polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig e3c
R&D Systems Lrig1 antibody (R&D, FAB3688G) was used in immunohistochemistry on mouse samples at 1:200 (fig e3c). Nature (2018) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:50; loading ...; fig s6d
In order to elucidate how cancers are distinguished from wounds, R&D Systems Lrig1 antibody (R&D Systems, AF3688) was used in immunohistochemistry on mouse samples at 1:50 (fig s6d). Cell (2017) ncbi
goat polyclonal
  • flow cytometry; mouse; loading ...; tbl 2
In order to describe how to isolate, culture, and characterize keratinocytes from murine skin, R&D Systems Lrig1 antibody (R&D Systems, AF3688) was used in flow cytometry on mouse samples (tbl 2). Methods Mol Biol (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; loading ...; fig 4a
In order to assess the expression of Lrig1 in senescent atrophic human epidermis and in the epidermis of CD44 knockout mice, R&D Systems Lrig1 antibody (R&D, AF3688) was used in immunohistochemistry on mouse samples (fig 4a). PLoS ONE (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:250; fig 1b-d
In order to test if LRIG1 contributes to the ontogeny of interstitial cells of Cajal in the small and large intestines, R&D Systems Lrig1 antibody (R&D systems, AF3688) was used in immunohistochemistry on mouse samples at 1:250 (fig 1b-d). Gastroenterology (2015) ncbi
goat polyclonal
  • western blot; mouse; 1:250
In order to study duodenal adenomas in leucine-rich repeats and immunoglobulin-like domains 1 -/- mice, R&D Systems Lrig1 antibody (R&D systems, AF3688) was used in western blot on mouse samples at 1:250. Am J Pathol (2015) ncbi
goat polyclonal
  • flow cytometry; mouse; 1:250
In order to assess the differences between two Lrig1 antibodies using intestinal samples, R&D Systems Lrig1 antibody (R&D Systems, FAB3688G) was used in flow cytometry on mouse samples at 1:250. Stem Cell Res (2014) ncbi
goat polyclonal
  • immunoprecipitation; mouse
  • immunohistochemistry; mouse; 1:100
  • western blot; mouse; 1:300
In order to assess the differences between two Lrig1 antibodies using intestinal samples, R&D Systems Lrig1 antibody (R&D Systems, AF3688) was used in immunoprecipitation on mouse samples , in immunohistochemistry on mouse samples at 1:100 and in western blot on mouse samples at 1:300. Stem Cell Res (2014) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse
R&D Systems Lrig1 antibody (R&D Systems, AF3688) was used in immunohistochemistry - frozen section on mouse samples . PLoS Genet (2012) ncbi
Articles Reviewed
  1. Liakath Ali K, Mills E, Sequeira I, Lichtenberger B, Pisco A, Sipilä K, et al. An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis. Nature. 2018;556:376-380 pubmed publisher
  2. Ge Y, Gomez N, Adam R, Nikolova M, Yang H, Verma A, et al. Stem Cell Lineage Infidelity Drives Wound Repair and Cancer. Cell. 2017;169:636-650.e14 pubmed publisher
  3. Moestrup K, Andersen M, Jensen K. Isolation and In Vitro Characterization of Epidermal Stem Cells. Methods Mol Biol. 2017;1553:67-83 pubmed publisher
  4. Barnes L, Saurat J, Kaya G. Senescent Atrophic Epidermis Retains Lrig1+ Stem Cells and Loses Wnt Signaling, a Phenotype Shared with CD44KO Mice. PLoS ONE. 2017;12:e0169452 pubmed publisher
  5. Kondo J, Powell A, Wang Y, Musser M, Southard Smith E, Franklin J, et al. LRIG1 Regulates Ontogeny of Smooth Muscle-Derived Subsets of Interstitial Cells of Cajal in Mice. Gastroenterology. 2015;149:407-19.e8 pubmed publisher
  6. Wang Y, Shi C, Lu Y, Poulin E, Franklin J, Coffey R. Loss of Lrig1 leads to expansion of Brunner glands followed by duodenal adenomas with gastric metaplasia. Am J Pathol. 2015;185:1123-34 pubmed publisher
  7. Poulin E, Powell A, Wang Y, Li Y, Franklin J, Coffey R. Using a new Lrig1 reporter mouse to assess differences between two Lrig1 antibodies in the intestine. Stem Cell Res. 2014;13:422-30 pubmed publisher
  8. Maddox J, Shakya A, South S, Shelton D, Andersen J, Chidester S, et al. Transcription factor Oct1 is a somatic and cancer stem cell determinant. PLoS Genet. 2012;8:e1003048 pubmed publisher