This is a Validated Antibody Database (VAD) review about mouse Lck, based on 30 published articles (read how Labome selects the articles), using Lck antibody in all methods. It is aimed to help Labome visitors find the most suited Lck antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Lck synonym: Hck-3; Lsk; Lskt; p56; p56Lck

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1b
Cell Signaling Technology Lck antibody (Cell signaling, 2752) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1b). Front Immunol (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3A5)
  • western blot; mouse; loading ...; fig 6a
Santa Cruz Biotechnology Lck antibody (Santa, sc-433) was used in western blot on mouse samples (fig 6a). Nat Immunol (2018) ncbi
mouse monoclonal (3A5)
  • western blot; human; loading ...; fig s7
Santa Cruz Biotechnology Lck antibody (SantaCruz, 3A5) was used in western blot on human samples (fig s7). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (3A5)
  • western blot; human; loading ...; fig 3c
Santa Cruz Biotechnology Lck antibody (Santa Cruz, SC-433) was used in western blot on human samples (fig 3c). Redox Biol (2017) ncbi
mouse monoclonal (3A5)
  • immunocytochemistry; human; loading ...; fig 1a
Santa Cruz Biotechnology Lck antibody (Santa Cruz, 3A5) was used in immunocytochemistry on human samples (fig 1a). J Immunol (2016) ncbi
mouse monoclonal (3A5)
  • flow cytometry; human; fig 1c
Santa Cruz Biotechnology Lck antibody (SantaCruz, 3A5) was used in flow cytometry on human samples (fig 1c). J Virol (2016) ncbi
mouse monoclonal (3A5)
  • immunocytochemistry; human; fig 4
Santa Cruz Biotechnology Lck antibody (Santa Cruz, sc-433) was used in immunocytochemistry on human samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (3A5)
  • western blot; human; loading ...; fig 3a
In order to test if the two hematopoietic systems in patients with mixed chimerism remain functional and study immunological differences, Santa Cruz Biotechnology Lck antibody (Santa Cruz, 3A5) was used in western blot on human samples (fig 3a). PLoS ONE (2016) ncbi
mouse monoclonal (3A5)
  • western blot; mouse; fig 5
Santa Cruz Biotechnology Lck antibody (Santa Cruz, 3A5) was used in western blot on mouse samples (fig 5). J Exp Med (2016) ncbi
mouse monoclonal (3A5)
  • western blot; mouse; fig 6
Santa Cruz Biotechnology Lck antibody (Santa Cruz, sc-433) was used in western blot on mouse samples (fig 6). Oncogene (2016) ncbi
mouse monoclonal (3A5)
  • western blot; mouse; fig s4
Santa Cruz Biotechnology Lck antibody (santa Cruz, sc-433) was used in western blot on mouse samples (fig s4). Front Cell Neurosci (2015) ncbi
mouse monoclonal (3A5)
  • western blot; mouse; fig 6a
Santa Cruz Biotechnology Lck antibody (Santa Cruz Biotechnology, 3A5) was used in western blot on mouse samples (fig 6a). J Exp Med (2015) ncbi
mouse monoclonal (3A5)
  • western blot; mouse; fig 1
Santa Cruz Biotechnology Lck antibody (Santa Cruz, sc-433) was used in western blot on mouse samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (3A5)
  • western blot; human; loading ...; fig 1c
Santa Cruz Biotechnology Lck antibody (Santa Cruz, sc-433) was used in western blot on human samples (fig 1c). PLoS ONE (2014) ncbi
mouse monoclonal (3A5)
  • western blot; human; fig 3
In order to show that CKIP-1 interacts with CARMA1 and inhibits PKCtheta-CBM-NF-kappaB signaling, Santa Cruz Biotechnology Lck antibody (Santa, sc-433) was used in western blot on human samples (fig 3). PLoS ONE (2014) ncbi
mouse monoclonal (3A5)
  • western blot; human
Santa Cruz Biotechnology Lck antibody (Santa Cruz Biotechnology, 3A5) was used in western blot on human samples . Mol Cell Biol (2014) ncbi
mouse monoclonal (3A5)
  • western blot; human
Santa Cruz Biotechnology Lck antibody (Santa Cruz, 3A5) was used in western blot on human samples . J Immunol (2013) ncbi
mouse monoclonal (3A5)
  • western blot; human; fig 1
Santa Cruz Biotechnology Lck antibody (Santa Cruz, sc-433) was used in western blot on human samples (fig 1). BMC Immunol (2009) ncbi
Invitrogen
mouse monoclonal (SRRCHA)
  • flow cytometry; mouse; 1:10; fig 2j
Invitrogen Lck antibody (Invitrogen, 46-9076-42) was used in flow cytometry on mouse samples at 1:10 (fig 2j). Nat Immunol (2019) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6b
Cell Signaling Technology Lck antibody (CST, 2751) was used in western blot on mouse samples at 1:1000 (fig 6b). PLoS Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1b
Cell Signaling Technology Lck antibody (Cell signaling, 2752) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1b). Front Immunol (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6c
Cell Signaling Technology Lck antibody (Cell Signaling, 2752) was used in western blot on mouse samples at 1:1000 (fig 6c). Nat Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6c
Cell Signaling Technology Lck antibody (Cell Signaling, 2751) was used in western blot on mouse samples at 1:1000 (fig 6c). Nat Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 7h
Cell Signaling Technology Lck antibody (Cell Signaling, 2751) was used in western blot on human samples (fig 7h). Nat Immunol (2018) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology Lck antibody (Cell Signaling, 2752) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6a
Cell Signaling Technology Lck antibody (Cell Signaling, 2752) was used in western blot on mouse samples at 1:1000 (fig 6a). J Immunol (2018) ncbi
domestic rabbit polyclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology Lck antibody (CST, 2752) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s6a
In order to demonstrate that PD-1 inhibits T cell function primarily by inactivating CD28 signaling, Cell Signaling Technology Lck antibody (Cell Signaling, 2751) was used in western blot on human samples (fig s6a). Science (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...
In order to analyze the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches, Cell Signaling Technology Lck antibody (Cell Signaling Technology, 2752) was used in western blot on human samples . Cell Syst (2017) ncbi
domestic rabbit polyclonal
  • flow cytometry; mouse; fig ev5
Cell Signaling Technology Lck antibody (Cell Signaling Tech, 2751) was used in flow cytometry on mouse samples (fig ev5). Mol Syst Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; fig 9
  • western blot; human; 1:1000; fig 9
In order to research the drive of early signaling and vesicle dynamics during T-cell activation by Aurora A, Cell Signaling Technology Lck antibody (Cell signaling, 2752) was used in immunocytochemistry on human samples at 1:200 (fig 9) and in western blot on human samples at 1:1000 (fig 9). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 5
Cell Signaling Technology Lck antibody (Cell Signaling, 2751) was used in western blot on mouse samples (fig 5). J Exp Med (2016) ncbi
BD Biosciences
mouse monoclonal (28/Lck)
  • western blot; human; loading ...; fig 4a
In order to report the proteome of CADA-treated SUP-T1 human CD4+ T lymphocytes, BD Biosciences Lck antibody (BD Biosciences, 28) was used in western blot on human samples (fig 4a). Mol Cell Proteomics (2017) ncbi
mouse monoclonal (4/LCK-Y505)
  • flow cytometry; human
In order to study NK function in solid tumors, BD Biosciences Lck antibody (BD Biosciences, 4/LCK-Y505) was used in flow cytometry on human samples . Int J Cancer (2014) ncbi
Articles Reviewed
  1. Sripada A, Sirohi K, Michalec L, Guo L, McKay J, Yadav S, et al. Sprouty2 positively regulates T cell function and airway inflammation through regulation of CSK and LCK kinases. PLoS Biol. 2021;19:e3001063 pubmed publisher
  2. Märklin M, Fuchs A, Tandler C, Heitmann J, Salih H, Kauer J, et al. Genetic Loss of LCK Kinase Leads to Acceleration of Chronic Lymphocytic Leukemia. Front Immunol. 2020;11:1995 pubmed publisher
  3. Verma V, Shrimali R, Ahmad S, Dai W, Wang H, Lu S, et al. PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance. Nat Immunol. 2019;20:1231-1243 pubmed publisher
  4. Kim H, Mun Y, Lee K, Park Y, Park J, Park J, et al. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells. Nat Commun. 2018;9:3630 pubmed publisher
  5. Borlido J, Sakuma S, Raices M, Carrette F, Tinoco R, Bradley L, et al. Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol. 2018;19:594-605 pubmed publisher
  6. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  7. Steinbuck M, Arakcheeva K, Winandy S. Novel TCR-Mediated Mechanisms of Notch Activation and Signaling. J Immunol. 2018;200:997-1007 pubmed publisher
  8. Li L, Guo X, Shi X, Li C, Wu W, Yan C, et al. Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling. Proc Natl Acad Sci U S A. 2017;114:E5891-E5899 pubmed publisher
  9. Chattopadhyay R, Raghavan S, Rao G. Resolvin D1 via prevention of ROS-mediated SHP2 inactivation protects endothelial adherens junction integrity and barrier function. Redox Biol. 2017;12:438-455 pubmed publisher
  10. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  11. Hui E, Cheung J, Zhu J, Su X, Taylor M, Wallweber H, et al. T cell costimulatory receptor CD28 is a primary target for PD-1-mediated inhibition. Science. 2017;355:1428-1433 pubmed publisher
  12. Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
  13. Van Puyenbroeck V, Claeys E, Schols D, Bell T, Vermeire K. A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA). Mol Cell Proteomics. 2017;16:157-167 pubmed publisher
  14. Silva J, Martins N, Henriques R, Soares H. HIV-1 Nef Impairs the Formation of Calcium Membrane Territories Controlling the Signaling Nanoarchitecture at the Immunological Synapse. J Immunol. 2016;197:4042-4052 pubmed
  15. Trautz B, Pierini V, Wombacher R, Stolp B, Chase A, Pizzato M, et al. The Antagonism of HIV-1 Nef to SERINC5 Particle Infectivity Restriction Involves the Counteraction of Virion-Associated Pools of the Restriction Factor. J Virol. 2016;90:10915-10927 pubmed publisher
  16. Pageon S, Tabarin T, Yamamoto Y, Ma Y, Nicovich P, Bridgeman J, et al. Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination. Proc Natl Acad Sci U S A. 2016;113:E5454-63 pubmed publisher
  17. Voisinne G, García Blesa A, Chaoui K, Fiore F, Bergot E, Girard L, et al. Co-recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR-induced ubiquitylation. Mol Syst Biol. 2016;12:876 pubmed publisher
  18. Stikvoort A, Sundin M, Uzunel M, Gertow J, Sundberg B, Schaffer M, et al. Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?. PLoS ONE. 2016;11:e0154737 pubmed publisher
  19. Blas Rus N, Bustos Morán E, Perez de Castro I, de Carcer G, Borroto A, Camafeita E, et al. Aurora A drives early signalling and vesicle dynamics during T-cell activation. Nat Commun. 2016;7:11389 pubmed publisher
  20. Hu H, Wang H, Xiao Y, Jin J, Chang J, Zou Q, et al. Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination. J Exp Med. 2016;213:399-414 pubmed publisher
  21. Franciosa G, Diluvio G, Gaudio F, Giuli M, Palermo R, Grazioli P, et al. Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression. Oncogene. 2016;35:4741-51 pubmed publisher
  22. De Mario A, Castellani A, Peggion C, Massimino M, Lim D, Hill A, et al. The prion protein constitutively controls neuronal store-operated Ca(2+) entry through Fyn kinase. Front Cell Neurosci. 2015;9:416 pubmed publisher
  23. Zou Q, Jin J, Xiao Y, Hu H, Zhou X, Jie Z, et al. T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi. J Exp Med. 2015;212:1323-36 pubmed publisher
  24. Silva O, Crocetti J, Humphries L, Burkhardt J, Miceli M. Discs Large Homolog 1 Splice Variants Regulate p38-Dependent and -Independent Effector Functions in CD8+ T Cells. PLoS ONE. 2015;10:e0133353 pubmed publisher
  25. Baens M, Bonsignore L, Somers R, Vanderheydt C, Weeks S, Gunnarsson J, et al. MALT1 auto-proteolysis is essential for NF-κB-dependent gene transcription in activated lymphocytes. PLoS ONE. 2014;9:e103774 pubmed publisher
  26. Prinz P, Mendler A, Brech D, Masouris I, Oberneder R, Noessner E. NK-cell dysfunction in human renal carcinoma reveals diacylglycerol kinase as key regulator and target for therapeutic intervention. Int J Cancer. 2014;135:1832-41 pubmed publisher
  27. Sakamoto T, Kobayashi M, Tada K, Shinohara M, Io K, Nagata K, et al. CKIP-1 is an intrinsic negative regulator of T-cell activation through an interaction with CARMA1. PLoS ONE. 2014;9:e85762 pubmed publisher
  28. Kloog Y, Mor A. Cytotoxic-T-lymphocyte antigen 4 receptor signaling for lymphocyte adhesion is mediated by C3G and Rap1. Mol Cell Biol. 2014;34:978-88 pubmed publisher
  29. Paster W, Brockmeyer C, Fu G, Simister P, de Wet B, Martinez Riaño A, et al. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J Immunol. 2013;190:3749-56 pubmed publisher
  30. Ngai J, Inngjerdingen M, Berge T, Tasken K. Interplay between the heterotrimeric G-protein subunits Galphaq and Galphai2 sets the threshold for chemotaxis and TCR activation. BMC Immunol. 2009;10:27 pubmed publisher