This is a Validated Antibody Database (VAD) review about mouse Irak1, based on 27 published articles (read how Labome selects the articles), using Irak1 antibody in all methods. It is aimed to help Labome visitors find the most suited Irak1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Irak1 synonym: AA408924; IRAK; IRAK-1; IRAK1-S; IRAK1b; Il1rak; Plpk; mPLK

Santa Cruz Biotechnology
mouse monoclonal (F-4)
  • immunohistochemistry; mouse; 1:50; loading ...; fig 5e
Santa Cruz Biotechnology Irak1 antibody (Santa Cruz Bio, sc-5288) was used in immunohistochemistry on mouse samples at 1:50 (fig 5e). Aging Dis (2021) ncbi
mouse monoclonal (F-4)
  • western blot; mouse; 1:200; loading ...; fig 7c
Santa Cruz Biotechnology Irak1 antibody (Santa, sc-5288) was used in western blot on mouse samples at 1:200 (fig 7c). Nat Commun (2019) ncbi
mouse monoclonal (F-4)
  • western blot; mouse; 1:200; loading ...; fig 7d
Santa Cruz Biotechnology Irak1 antibody (Santa Cruz, sc-5288) was used in western blot on mouse samples at 1:200 (fig 7d). F1000Res (2017) ncbi
mouse monoclonal (F-4)
  • western blot; human; 1:500; fig 4c
Santa Cruz Biotechnology Irak1 antibody (SantaCruz, 5288) was used in western blot on human samples at 1:500 (fig 4c). Nat Cell Biol (2015) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 7b
Abcam Irak1 antibody (Abcam, ab238) was used in western blot on mouse samples at 1:1000 (fig 7b). Cell Death Discov (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:200; fig 1
  • immunocytochemistry; mouse; 1:200; fig 3
Abcam Irak1 antibody (Abcam, ab238) was used in immunohistochemistry - free floating section on mouse samples at 1:200 (fig 1) and in immunocytochemistry on mouse samples at 1:200 (fig 3). Nat Commun (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; 1:1000; loading ...; fig 3d, s1c
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on mouse samples at 1:1000 (fig 3d, s1c). Nat Commun (2022) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; fig 3b
Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, 4504) was used in western blot on mouse samples (fig 3b). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; loading ...; fig 3a
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on mouse samples (fig 3a). Nat Immunol (2020) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 2g
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on human samples (fig 2g). Nat Commun (2019) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; loading ...; fig s11a
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on mouse samples (fig s11a). Sci Signal (2019) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 5g
Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, 4504) was used in western blot on human samples (fig 5g). Nat Cell Biol (2019) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; 1:1000; loading ...; fig 3a
Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, 4504) was used in western blot on mouse samples at 1:1000 (fig 3a). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 3d
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504S) was used in western blot on human samples (fig 3d). J Virol (2018) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; loading ...; fig 8a
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504S) was used in western blot on mouse samples (fig 8a). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 6a
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on human samples (fig 6a). J Virol (2017) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig s1d
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on human samples (fig s1d). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (D51G7)
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used . Sci Rep (2016) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; loading ...; fig 2h
In order to investigate how PI3Kgamma regulates macrophage polarization, Cell Signaling Technology Irak1 antibody (Cell Signaling, D51G7) was used in western blot on mouse samples (fig 2h). Nature (2016) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; loading ...; fig 6c
Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, D51G7) was used in western blot on human samples (fig 6c). Cell Rep (2016) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on human samples . Biochem Biophys Res Commun (2016) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; fig 8
  • western blot; human; fig 7
In order to identify species-specific preferences in signaling protein use by comprehensive RNAi-based screening of human and mouse TLR pathways, Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on mouse samples (fig 8) and in western blot on human samples (fig 7). Sci Signal (2016) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; 1:500; fig 3
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in western blot on mouse samples at 1:500 (fig 3). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human
Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, 4504) was used in western blot on human samples . Mol Biol Cell (2015) ncbi
domestic rabbit monoclonal (D51G7)
  • flow cytometry; human; loading ...; fig 4b
Cell Signaling Technology Irak1 antibody (Cell Signaling, 4504) was used in flow cytometry on human samples (fig 4b). Invest New Drugs (2015) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; human; 1:10,000
In order to identify an epigenetic mechanism of crosstalk between STAT3 and NF-kappaB that regulates constitutive STAT3 activation in oncogenesis, Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, 4504) was used in western blot on human samples at 1:10,000. Sci Signal (2014) ncbi
domestic rabbit monoclonal (D51G7)
  • western blot; mouse; fig 6c
Cell Signaling Technology Irak1 antibody (Cell Signaling Technology, 4504) was used in western blot on mouse samples (fig 6c). J Immunol (2012) ncbi
Articles Reviewed
  1. Liu W, Zhou H, Wang H, Zhang Q, Zhang R, Willard B, et al. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nat Commun. 2022;13:2748 pubmed publisher
  2. Lu J, Wang W, Li P, Wang X, Gao C, Zhang B, et al. MiR-146a regulates regulatory T cells to suppress heart transplant rejection in mice. Cell Death Discov. 2021;7:165 pubmed publisher
  3. An L, Shen Y, Chopp M, Zacharek A, Venkat P, Chen Z, et al. Deficiency of Endothelial Nitric Oxide Synthase (eNOS) Exacerbates Brain Damage and Cognitive Deficit in A Mouse Model of Vascular Dementia. Aging Dis. 2021;12:732-746 pubmed publisher
  4. Watahiki A, Hoshikawa S, Chiba M, Egusa H, Fukumoto S, Inuzuka H. Deficiency of Lipin2 Results in Enhanced NF-κB Signaling and Osteoclast Formation in RAW-D Murine Macrophages. Int J Mol Sci. 2021;22: pubmed publisher
  5. Zhou H, Wang H, Yu M, Schugar R, Qian W, Tang F, et al. IL-1 induces mitochondrial translocation of IRAK2 to suppress oxidative metabolism in adipocytes. Nat Immunol. 2020;21:1219-1231 pubmed publisher
  6. Managò A, Audrito V, Mazzola F, Sorci L, Gaudino F, Gizzi K, et al. Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation. Nat Commun. 2019;10:4116 pubmed publisher
  7. Ben J, Jiang B, Wang D, Liu Q, Zhang Y, Qi Y, et al. Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK-NF-κB signaling mediated inflammation. Nat Commun. 2019;10:1801 pubmed publisher
  8. Herb M, Gluschko A, Wiegmann K, Farid A, Wolf A, Utermöhlen O, et al. Mitochondrial reactive oxygen species enable proinflammatory signaling through disulfide linkage of NEMO. Sci Signal. 2019;12: pubmed publisher
  9. Liu P, Shah R, Li Y, Arora A, Ung P, Raman R, et al. An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy. Nat Cell Biol. 2019;21:203-213 pubmed publisher
  10. Zhang C, Jiang M, Zhou H, Liu W, Wang C, Kang Z, et al. TLR-stimulated IRAKM activates caspase-8 inflammasome in microglia and promotes neuroinflammation. J Clin Invest. 2018;128:5399-5412 pubmed publisher
  11. Xu W, Ma C, Zhang Q, Zhao R, Hu D, Zhang X, et al. PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. J Virol. 2018;92: pubmed publisher
  12. McCarthy G, Bridges C, Blednov Y, Harris R. CNS cell-type localization and LPS response of TLR signaling pathways. F1000Res. 2017;6:1144 pubmed publisher
  13. Li B, Wang X, Choi I, Wang Y, Liu S, Pham A, et al. miR-146a modulates autoreactive Th17 cell differentiation and regulates organ-specific autoimmunity. J Clin Invest. 2017;127:3702-3716 pubmed publisher
  14. Franz S, Rennert P, Woznik M, Grützke J, Lüdde A, Arriero Pais E, et al. Mumps Virus SH Protein Inhibits NF-κB Activation by Interacting with Tumor Necrosis Factor Receptor 1, Interleukin-1 Receptor 1, and Toll-Like Receptor 3 Complexes. J Virol. 2017;91: pubmed publisher
  15. Strickson S, Emmerich C, Goh E, Zhang J, Kelsall I, MacArtney T, et al. Roles of the TRAF6 and Pellino E3 ligases in MyD88 and RANKL signaling. Proc Natl Acad Sci U S A. 2017;114:E3481-E3489 pubmed publisher
  16. Boro M, Singh V, Balaji K. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses. Sci Rep. 2016;6:37695 pubmed publisher
  17. Kaneda M, Messer K, Ralainirina N, Li H, Leem C, Gorjestani S, et al. PI3Kγ is a molecular switch that controls immune suppression. Nature. 2016;539:437-442 pubmed publisher
  18. Hedl M, Yan J, Abraham C. IRF5 and IRF5 Disease-Risk Variants Increase Glycolysis and Human M1 Macrophage Polarization by Regulating Proximal Signaling and Akt2 Activation. Cell Rep. 2016;16:2442-55 pubmed publisher
  19. Emmerich C, Bakshi S, Kelsall I, Ortiz Guerrero J, Shpiro N, Cohen P. Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling. Biochem Biophys Res Commun. 2016;474:452-461 pubmed publisher
  20. Kishi N, MacDonald J, Ye J, Molyneaux B, Azim E, Macklis J. Reduction of aberrant NF-κB signalling ameliorates Rett syndrome phenotypes in Mecp2-null mice. Nat Commun. 2016;7:10520 pubmed publisher
  21. Sun J, Li N, Oh K, Dutta B, Vayttaden S, Lin B, et al. Comprehensive RNAi-based screening of human and mouse TLR pathways identifies species-specific preferences in signaling protein use. Sci Signal. 2016;9:ra3 pubmed publisher
  22. Laberge R, Sun Y, Orjalo A, Patil C, Freund A, Zhou L, et al. MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation. Nat Cell Biol. 2015;17:1049-61 pubmed publisher
  23. Alexander M, Hu R, Runtsch M, Kagele D, Mosbruger T, Tolmachova T, et al. Exosome-delivered microRNAs modulate the inflammatory response to endotoxin. Nat Commun. 2015;6:7321 pubmed publisher
  24. Willy J, Young S, Stevens J, Masuoka H, Wek R. CHOP links endoplasmic reticulum stress to NF-κB activation in the pathogenesis of nonalcoholic steatohepatitis. Mol Biol Cell. 2015;26:2190-204 pubmed publisher
  25. Guzmán E, Maers K, Roberts J, Kemami Wangun H, Harmody D, Wright A. The marine natural product microsclerodermin A is a novel inhibitor of the nuclear factor kappa B and induces apoptosis in pancreatic cancer cells. Invest New Drugs. 2015;33:86-94 pubmed publisher
  26. Xiang M, Birkbak N, Vafaizadeh V, Walker S, Yeh J, Liu S, et al. STAT3 induction of miR-146b forms a feedback loop to inhibit the NF-?B to IL-6 signaling axis and STAT3-driven cancer phenotypes. Sci Signal. 2014;7:ra11 pubmed publisher
  27. Xu L, Jin L, Zhang B, Akerlund L, Shu H, Cambier J. VISA is required for B cell expression of TLR7. J Immunol. 2012;188:248-58 pubmed publisher