This is a Validated Antibody Database (VAD) review about mouse Ilk, based on 23 published articles (read how Labome selects the articles), using Ilk antibody in all methods. It is aimed to help Labome visitors find the most suited Ilk antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ilk synonym: AA511515; ESTM24; integrin-linked protein kinase; integrin binding protein kinase

Knockout validation
Cell Signaling Technology
rabbit monoclonal (4G9)
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 9a
Cell Signaling Technology Ilk antibody (Cell Signaling, 3856) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1c), in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1c) and in western blot on mouse samples at 1:1000 (fig 9a). J Neurosci (2017) ncbi
Abcam
rabbit monoclonal (EPR1592)
  • western blot; human; loading ...; fig 4a
Abcam Ilk antibody (Abcam, ab76468) was used in western blot on human samples (fig 4a). Sci Rep (2017) ncbi
rabbit monoclonal (EP1593Y)
  • western blot; human; 1:100; fig s7
In order to analyze organotropic metasistasis and tumour exosome integrins, Abcam Ilk antibody (Abcam, ab52480) was used in western blot on human samples at 1:100 (fig s7). Nature (2015) ncbi
rabbit monoclonal (EP1593Y)
  • western blot; mouse; fig 2a
  • western blot; rat; fig 1
In order to generate and characterize PINCH 1 and PINCH 2 double knockout mice, Abcam Ilk antibody (abcam, ab52480) was used in western blot on mouse samples (fig 2a) and in western blot on rat samples (fig 1). PLoS ONE (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (65.1)
  • western blot; human; 1:200; loading ...; fig 5b
Santa Cruz Biotechnology Ilk antibody (SantaCruz, sc-20019) was used in western blot on human samples at 1:200 (fig 5b). Oncotarget (2016) ncbi
mouse monoclonal (65.1)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1b
Santa Cruz Biotechnology Ilk antibody (Santa Cruz, sc-20019) was used in immunohistochemistry - paraffin section on human samples (fig 1b). Cell Cycle (2015) ncbi
mouse monoclonal (65.1)
  • immunohistochemistry - paraffin section; human; 1:100
Santa Cruz Biotechnology Ilk antibody (Santa Cruz Biotechnology, SC-20019) was used in immunohistochemistry - paraffin section on human samples at 1:100. Tumour Biol (2014) ncbi
Abnova
mouse monoclonal (4F10)
  • western blot; human; 1:5000
Abnova Ilk antibody (Abnova, 4F10) was used in western blot on human samples at 1:5000. J Proteome Res (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (4G9)
  • western blot; human; loading ...; fig 3i
Cell Signaling Technology Ilk antibody (Cell Signaling, 3856) was used in western blot on human samples (fig 3i). Sci Rep (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 6a
In order to see if tetraspanin 8 influences cell-matrix anchorage and regulates downstream molecular pathways leading to aggressive behavior of tumor progression in melanoma, Cell Signaling Technology Ilk antibody (Cell Signaling, 3862) was used in western blot on human samples (fig 6a). Oncotarget (2017) ncbi
rabbit monoclonal (4G9)
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 9a
Cell Signaling Technology Ilk antibody (Cell Signaling, 3856) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1c), in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1c) and in western blot on mouse samples at 1:1000 (fig 9a). J Neurosci (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 5a
In order to test if OSU-T315 inhibits vestibular schwannoma and meningioma cell growth, Cell Signaling Technology Ilk antibody (Cell Signaling, 3862) was used in western blot on human samples at 1:1000 (fig 5a). Austin J Med Oncol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1
In order to learn about the control of differentiation in mammary epithelial cells by the integrin-mediated ILK-parvin-alpha-Pix signaling axis, Cell Signaling Technology Ilk antibody (Cell Signaling, 3862) was used in western blot on mouse samples (fig 1). J Cell Physiol (2016) ncbi
rabbit monoclonal (4G9)
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology Ilk antibody (Cell signaling, 3856) was used in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 2c). Oncotarget (2015) ncbi
rabbit polyclonal
  • western blot; rat
Cell Signaling Technology Ilk antibody (Cell Signaling Technology, 3862) was used in western blot on rat samples . Physiol Rep (2014) ncbi
BD Biosciences
mouse monoclonal (3/ILK)
  • immunohistochemistry; human; loading ...; fig 8d
  • western blot; human; loading ...; fig s8a
In order to study factors that allow tumor cells to metastasize, BD Biosciences Ilk antibody (BD, 611803) was used in immunohistochemistry on human samples (fig 8d) and in western blot on human samples (fig s8a). Mol Biol Cell (2017) ncbi
mouse monoclonal (3/ILK)
  • immunocytochemistry; mouse; 1:500; fig 3
  • western blot; mouse; 1:5000; fig 4
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, BD Biosciences Ilk antibody (Transduction Labs, 611803) was used in immunocytochemistry on mouse samples at 1:500 (fig 3) and in western blot on mouse samples at 1:5000 (fig 4). elife (2016) ncbi
mouse monoclonal (3/ILK)
  • western blot; rat; 1:1000; fig 5
BD Biosciences Ilk antibody (BD Biosciences, 61183) was used in western blot on rat samples at 1:1000 (fig 5). Int J Mol Med (2015) ncbi
mouse monoclonal (3/ILK)
  • western blot; human; 1:400; loading ...; fig 2b
BD Biosciences Ilk antibody (BD Biosciences, 611802) was used in western blot on human samples at 1:400 (fig 2b). Cell Cycle (2015) ncbi
mouse monoclonal (3/ILK)
  • western blot; human; 1:1000; fig 1
BD Biosciences Ilk antibody (BD Biosciences, 611802) was used in western blot on human samples at 1:1000 (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (3/ILK)
  • immunoprecipitation; hamsters; 1 ug/ml
  • western blot; hamsters; 0.125 ug/ml
BD Biosciences Ilk antibody (BD Biosciences, 3/ILK) was used in immunoprecipitation on hamsters samples at 1 ug/ml and in western blot on hamsters samples at 0.125 ug/ml. PLoS ONE (2013) ncbi
Sigma-Aldrich
mouse monoclonal (65.1)
  • western blot; mouse; 1:2000; fig s16
Sigma-Aldrich Ilk antibody (Sigma, 65.1) was used in western blot on mouse samples at 1:2000 (fig s16). Nat Commun (2016) ncbi
mouse monoclonal (4F10)
  • western blot; Domestic guinea pig; 1:500; fig 6
In order to assess the independence of secretion of beta-1 integrins in airway wall and focal adhesions proteins down regulation from airway hyperresponsiveness in asthma model, Sigma-Aldrich Ilk antibody (Sigma, WH0003611M1) was used in western blot on Domestic guinea pig samples at 1:500 (fig 6). J Cell Biochem (2016) ncbi
mouse monoclonal (65.1)
  • western blot; mouse; 1:1000; fig 5
Sigma-Aldrich Ilk antibody (Cell Signaling, I0783) was used in western blot on mouse samples at 1:1000 (fig 5). Nat Commun (2015) ncbi
mouse monoclonal (65.1)
  • immunocytochemistry; human; 1:800; fig 2
Sigma-Aldrich Ilk antibody (Sigma, 65.1) was used in immunocytochemistry on human samples at 1:800 (fig 2). Sci Rep (2015) ncbi
mouse monoclonal (65.1)
  • immunohistochemistry; mouse; fig s1
Sigma-Aldrich Ilk antibody (Sigma, I0783) was used in immunohistochemistry on mouse samples (fig s1). BMC Biol (2013) ncbi
Articles Reviewed
  1. Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed publisher
  2. Merhi A, Delree P, Marini A. The metabolic waste ammonium regulates mTORC2 and mTORC1 signaling. Sci Rep. 2017;7:44602 pubmed publisher
  3. El Kharbili M, Robert C, Witkowski T, Danty Berger E, Barbollat Boutrand L, Masse I, et al. Tetraspanin 8 is a novel regulator of ILK-driven ?1 integrin adhesion and signaling in invasive melanoma cells. Oncotarget. 2017;8:17140-17155 pubmed publisher
  4. Hussain R, Macklin W. Integrin-Linked Kinase (ILK) Deletion Disrupts Oligodendrocyte Development by Altering Cell Cycle. J Neurosci. 2017;37:397-412 pubmed publisher
  5. Su Y, Hiemstra T, Yan Y, Li J, Karet H, Rosen L, et al. PDLIM5 links kidney anion exchanger 1 (kAE1) to ILK and is required for membrane targeting of kAE1. Sci Rep. 2017;7:39701 pubmed publisher
  6. Mercado Pimentel M, Igarashi S, Dunn A, Behbahani M, Miller C, Read C, et al. The Novel Small Molecule Inhibitor, OSU-T315, Suppresses Vestibular Schwannoma and Meningioma Growth by Inhibiting PDK2 Function in the AKT Pathway Activation. Austin J Med Oncol. 2016;3: pubmed
  7. Zhang C, Wang P, Liu D, Chen C, Zhao W, Chen X, et al. The molecular basis of the genesis of basal tone in internal anal sphincter. Nat Commun. 2016;7:11358 pubmed publisher
  8. Liu S, Zhou F, Shen Y, Zhang Y, Yin H, Zeng Y, et al. Fluid shear stress induces epithelial-mesenchymal transition (EMT) in Hep-2 cells. Oncotarget. 2016;7:32876-92 pubmed publisher
  9. Rooney N, Wang P, Brennan K, Gilmore A, Streuli C. The Integrin-Mediated ILK-Parvin-?Pix Signaling Axis Controls Differentiation in Mammary Epithelial Cells. J Cell Physiol. 2016;231:2408-17 pubmed publisher
  10. Álvarez Santos M, Carbajal V, Tellez Jiménez O, Martínez Cordero E, Ruiz V, Hernández Pando R, et al. Airway Hyperresponsiveness in Asthma Model Occurs Independently of Secretion of β1 Integrins in Airway Wall and Focal Adhesions Proteins Down Regulation. J Cell Biochem. 2016;117:2385-96 pubmed publisher
  11. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  12. Hoshino A, Costa Silva B, Shen T, Rodrigues G, Hashimoto A, Tesic Mark M, et al. Tumour exosome integrins determine organotropic metastasis. Nature. 2015;527:329-35 pubmed publisher
  13. Hausmann C, Temme A, Cordes N, Eke I. ILKAP, ILK and PINCH1 control cell survival of p53-wildtype glioblastoma cells after irradiation. Oncotarget. 2015;6:34592-605 pubmed publisher
  14. Yan G, Wang Q, Hu S, Wang D, Qiao Y, Ma G, et al. Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury. Int J Mol Med. 2015;36:1001-11 pubmed publisher
  15. Duminuco R, Noble J, Goody J, Sharma M, Ksander B, Roskelley C, et al. Integrin-linked kinase regulates senescence in an Rb-dependent manner in cancer cell lines. Cell Cycle. 2015;14:2924-37 pubmed publisher
  16. Wu C, Jiao H, Lai Y, Zheng W, Chen K, Qu H, et al. Kindlin-2 controls TGF-β signalling and Sox9 expression to regulate chondrogenesis. Nat Commun. 2015;6:7531 pubmed publisher
  17. Morris E, Assi K, Salh B, Dedhar S. Integrin-linked kinase links dynactin-1/dynactin-2 with cortical integrin receptors to orient the mitotic spindle relative to the substratum. Sci Rep. 2015;5:8389 pubmed publisher
  18. Ogasawara R, Nakazato K, Sato K, Boppart M, Fujita S. Resistance exercise increases active MMP and β1-integrin protein expression in skeletal muscle. Physiol Rep. 2014;2: pubmed publisher
  19. Genovese F, Gualandi A, Taddia L, Marverti G, Pirondi S, Marraccini C, et al. Mass spectrometric/bioinformatic identification of a protein subset that characterizes the cellular activity of anticancer peptides. J Proteome Res. 2014;13:5250-61 pubmed publisher
  20. Honda S, Shirotani Ikejima H, Tadokoro S, Tomiyama Y, Miyata T. The integrin-linked kinase-PINCH-parvin complex supports integrin ?IIb?3 activation. PLoS ONE. 2013;8:e85498 pubmed publisher
  21. Dai X, Jiang W, Zhang Q, Xu L, Geng P, Zhuang S, et al. Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis. BMC Biol. 2013;11:107 pubmed publisher
  22. Donthamsetty S, Bhave V, Mars W, Bowen W, Orr A, Haynes M, et al. Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis. PLoS ONE. 2013;8:e74625 pubmed publisher
  23. Mao X, Miao S, He H, Miao S, Pei R, Yang X, et al. Krüppel-like factor 5: a novel biomarker for lymph node metastasis and recurrence in supraglottic squamous cell laryngeal carcinoma. Tumour Biol. 2014;35:623-9 pubmed publisher