This is a Validated Antibody Database (VAD) review about mouse Il18, based on 13 published articles (read how Labome selects the articles), using Il18 antibody in all methods. It is aimed to help Labome visitors find the most suited Il18 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Il18 synonym: Igif; Il-18

Knockout validation
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 1b
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1b
Abcam Il18 antibody (Abcam, ab71495) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 1b) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 1b). Front Immunol (2019) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:200; loading ...; fig 1b
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1b
Abcam Il18 antibody (Abcam, ab71495) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig 1b) and in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 1b). Front Immunol (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 11c
Abcam Il18 antibody (Cell Signaling, ab71495) was used in western blot on mouse samples at 1:1000 (fig 11c). Int J Mol Med (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1a
Abcam Il18 antibody (Abcam, ab71495) was used in western blot on mouse samples (fig 1a). J Exp Med (2017) ncbi
domestic rabbit polyclonal
  • flow cytometry; mouse; fig 3d
Abcam Il18 antibody (Abcam, Ab71495) was used in flow cytometry on mouse samples (fig 3d). Immunity (2016) ncbi
R&D Systems
rat monoclonal (93-10C)
  • blocking or activating experiments; mouse; fig 8b
In order to study changes in IL-23 and IL-22 after scratching, R&D Systems Il18 antibody (R&D Systems, 93-10C) was used in blocking or activating experiments on mouse samples (fig 8b). J Exp Med (2016) ncbi
rat monoclonal (39-3F)
  • western blot; mouse; fig 5A
R&D Systems Il18 antibody (R&D, D046-3) was used in western blot on mouse samples (fig 5A). Oncoimmunology (2016) ncbi
Bio X Cell
rat monoclonal (YIGIF74-1G7)
  • blocking or activating experiments; mouse; loading ...; fig s4a
Bio X Cell Il18 antibody (BioXcell, YIG1F74-1G7) was used in blocking or activating experiments on mouse samples (fig s4a). J Exp Med (2017) ncbi
Rockland Immunochemicals
domestic rabbit polyclonal
  • immunocytochemistry; mouse; fig 2f
  • western blot; mouse; fig 2e
In order to characterize the innate immunity responses to TDP-43 aggregates, Rockland Immunochemicals Il18 antibody (rockland, 210-401-323) was used in immunocytochemistry on mouse samples (fig 2f) and in western blot on mouse samples (fig 2e). FASEB J (2017) ncbi
BioVision
  • western blot; mouse; 1:1000; fig s1
In order to study a signal amplification mechanism for inflammasomes through ASC filament formation, BioVision Il18 antibody (Biovision, 5180R-100) was used in western blot on mouse samples at 1:1000 (fig s1). Nat Commun (2016) ncbi
  • western blot; mouse; fig 3
BioVision Il18 antibody (Biovision, 5180) was used in western blot on mouse samples (fig 3). Sci Rep (2016) ncbi
  • western blot; mouse; fig 12d
In order to study development of progressive retinal degeneration in transgenic mice overexpressing serum retinol-binding protein in a retinoid-independent mechanism, BioVision Il18 antibody (BioVision, 5180R) was used in western blot on mouse samples (fig 12d). Mol Cell Biol (2015) ncbi
MBL International
monoclonal
  • ELISA; mouse; 1:1000; loading ...; fig 7I
In order to analyze the effects of a loss of autophagy in dendritic cells and B cells in a TLR7-mediated model of autoimmunity, MBL International Il18 antibody (MBL International, D047-3) was used in ELISA on mouse samples at 1:1000 (fig 7I). J Immunol (2017) ncbi
monoclonal
  • ELISA; mouse; 1:1000; loading ...; fig s5a
In order to find C57BL/6J mice have a defect in neutrophil recruitment to a range of inflammatory stimuli via NLRP12, MBL International Il18 antibody (MBL International, D047-3) was used in ELISA on mouse samples at 1:1000 (fig s5a). Nat Commun (2016) ncbi
monoclonal
  • ELISA; mouse; 1:2000; fig 1
In order to determine inhibition of NLRC4-mediated inflammasome activation and binding to annexin A2 by the tick protein sialostatin L2, MBL International Il18 antibody (MBL, D048-6) was used in ELISA on mouse samples at 1:2000 (fig 1). Infect Immun (2016) ncbi
monoclonal
  • ELISA; mouse; 1:1000; fig 1
In order to determine inhibition of NLRC4-mediated inflammasome activation and binding to annexin A2 by the tick protein sialostatin L2, MBL International Il18 antibody (MBL, D047-3) was used in ELISA on mouse samples at 1:1000 (fig 1). Infect Immun (2016) ncbi
Articles Reviewed
  1. Borghi M, Pariano M, Solito V, Puccetti M, Bellet M, Stincardini C, et al. Targeting the Aryl Hydrocarbon Receptor With Indole-3-Aldehyde Protects From Vulvovaginal Candidiasis via the IL-22-IL-18 Cross-Talk. Front Immunol. 2019;10:2364 pubmed publisher
  2. Song H, Li X, Liu Y, Lu W, Cui Z, Zhou L, et al. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS. Int J Mol Med. 2018;42:193-207 pubmed publisher
  3. Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
  4. Leal Lasarte M, Franco J, Labrador Garrido A, Pozo D, Roodveldt C. Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia. FASEB J. 2017;31:2797-2816 pubmed publisher
  5. Weindel C, Richey L, Mehta A, Shah M, Huber B. Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated Autoimmunity. J Immunol. 2017;198:1081-1092 pubmed publisher
  6. Ulland T, Jain N, Hornick E, Elliott E, Clay G, Sadler J, et al. Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. Nat Commun. 2016;7:13180 pubmed publisher
  7. Yoon J, Leyva Castillo J, Wang G, Galand C, Oyoshi M, Kumar L, et al. IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization. J Exp Med. 2016;213:2147-66 pubmed publisher
  8. Zhang M, Qi Y, Li H, Cui J, Dai L, Frank J, et al. AIM2 inflammasome mediates Arsenic-induced secretion of IL-1 ? and IL-18. Oncoimmunology. 2016;5:e1160182 pubmed publisher
  9. Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, et al. Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection. Immunity. 2016;45:131-44 pubmed publisher
  10. Dick M, Sborgi L, Rühl S, Hiller S, Broz P. ASC filament formation serves as a signal amplification mechanism for inflammasomes. Nat Commun. 2016;7:11929 pubmed publisher
  11. Pourcet B, Gage M, León T, Waddington K, Pello O, Steffensen K, et al. The nuclear receptor LXR modulates interleukin-18 levels in macrophages through multiple mechanisms. Sci Rep. 2016;6:25481 pubmed publisher
  12. Wang X, Shaw D, Sakhon O, Snyder G, Sundberg E, Santambrogio L, et al. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. Infect Immun. 2016;84:1796-1805 pubmed publisher
  13. Du M, Otalora L, Martin A, Moiseyev G, Vanlandingham P, Wang Q, et al. Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol. 2015;35:2771-89 pubmed publisher