This is a Validated Antibody Database (VAD) review about mouse Hspa1b, based on 30 published articles (read how Labome selects the articles), using Hspa1b antibody in all methods. It is aimed to help Labome visitors find the most suited Hspa1b antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Hspa1b synonym: HSP70B1; Hsp70; Hsp70-1; Hsp70.1; hsp68

Abcam
mouse monoclonal (C92F3A-5)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1f
  • immunocytochemistry; human; 1:100; loading ...; fig 2i
Abcam Hspa1b antibody (Abcam, ab47455) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1f) and in immunocytochemistry on human samples at 1:100 (fig 2i). Nat Cell Biol (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1i
Abcam Hspa1b antibody (Abcam, ab31010) was used in western blot on human samples at 1:1000 (fig 1i). Aging (Albany NY) (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig s6c
Abcam Hspa1b antibody (Abcam, ab79852) was used in western blot on mouse samples at 1:1000 (fig s6c). Sci Adv (2019) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; loading ...; fig s3b
In order to study the suppressive effect of DNAJB6 and Hsp70 on alpha-synuclein aggregation, Abcam Hspa1b antibody (Abcam, ab47455) was used in western blot on human samples (fig s3b). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4b
Abcam Hspa1b antibody (Abcam, ab79852) was used in western blot on human samples (fig 4b). Mol Syst Biol (2017) ncbi
mouse monoclonal (2A4)
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 1c
In order to examine the structural and ultra-structural changes induced by nitrate in rat liver and assess the protective role of s-allylcysteine, Abcam Hspa1b antibody (Abcam, ab5442) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 1c). Ultrastruct Pathol (2017) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:1000; fig 1
In order to research differentiation of functional glutamatergic neurons from placenta-derived multipotent cells by knocking down of heat-shock protein 27, Abcam Hspa1b antibody (Abcam, ab47455) was used in western blot on human samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; rat; 1:1000; loading ...; fig 3b
Abcam Hspa1b antibody (Abcam, ab47455) was used in western blot on rat samples at 1:1000 (fig 3b). In Vitro Cell Dev Biol Anim (2016) ncbi
mouse monoclonal (2A4)
  • immunohistochemistry - paraffin section; human; loading ...; tbl 2
Abcam Hspa1b antibody (Abcam, 2A4) was used in immunohistochemistry - paraffin section on human samples (tbl 2). J Transl Med (2016) ncbi
mouse monoclonal (C92F3A-5)
  • immunoprecipitation; human; loading ...; fig 2a
  • immunocytochemistry; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3c
Abcam Hspa1b antibody (Abcam, ab47455) was used in immunoprecipitation on human samples (fig 2a), in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3c). FASEB J (2016) ncbi
mouse monoclonal (2A4)
  • western blot; rat; 1:1000; fig 1
Abcam Hspa1b antibody (Abcam, ab5442) was used in western blot on rat samples at 1:1000 (fig 1). Int J Mol Med (2016) ncbi
mouse monoclonal (2A4)
  • ELISA; Austrofundulus limnaeus; 1:1000; loading ...; fig 6a
In order to analyze chromatin in vertebrate embryo diapause, Abcam Hspa1b antibody (Abcam, ab5442) was used in ELISA on Austrofundulus limnaeus samples at 1:1000 (fig 6a). J Exp Biol (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:100
In order to show that that both cell-mediated and humoral adaptive immune components are involved in photodynamic therapy, Abcam Hspa1b antibody (Abcam, ab31010) was used in immunocytochemistry on mouse samples at 1:100. Cell Mol Immunol (2017) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; African green monkey
In order to perform a proteomic analysis of host proteins in Rift Valley fever virus virions and to study the particular significance of Hsp90, Abcam Hspa1b antibody (Abcam, ab47455) was used in western blot on African green monkey samples . PLoS ONE (2014) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; fig s3b
Abcam Hspa1b antibody (Abcam, ab47455) was used in western blot on human samples (fig s3b). PLoS ONE (2012) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig s3b
Cell Signaling Technology Hspa1b antibody (CST, 4872) was used in western blot on human samples at 1:500 (fig s3b). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s1c
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872) was used in western blot on mouse samples (fig s1c). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6g
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872) was used in western blot on human samples (fig 6g). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3b
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4876) was used in western blot on mouse samples (fig 3b). Life Sci (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s5e
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872) was used in western blot on human samples (fig s5e). Cell (2018) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 4b
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872) was used in western blot on rat samples (fig 4b). J Biol Chem (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s2b
  • immunohistochemistry; mouse; loading ...; fig s3c
  • western blot; mouse; loading ...; fig s3a
In order to investigate the involvement of HspBP1 in the neuronal susceptibility towards misfolded proteins, Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872S) was used in western blot on human samples (fig s2b), in immunohistochemistry on mouse samples (fig s3c) and in western blot on mouse samples (fig s3a). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology Hspa1b antibody (cell signalling, 4876) was used in western blot on human samples (fig 2a). Oxid Med Cell Longev (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3b, 3c
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872) was used in western blot on human samples (fig 3b, 3c). Mol Cell (2017) ncbi
domestic rabbit polyclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology Hspa1b antibody (CST, 4872) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology Hspa1b antibody (Cell Signalling, 4872) was used in western blot on human samples (fig 2a). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; loading ...; fig 4a
  • western blot; rat; 1:1000; loading ...; fig 1a
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4872S) was used in immunohistochemistry - paraffin section on rat samples (fig 4a) and in western blot on rat samples at 1:1000 (fig 1a). Mol Med Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3c
Cell Signaling Technology Hspa1b antibody (Cell Signaling, 4876) was used in western blot on human samples (fig 3c). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • flow cytometry; human; 1:25; fig 1b
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; 1:500; fig 1d
In order to assess the effect of shepherdin, a peptido-mimetic that inhibits the heat shock protein-Survivin interaction, in retinoblastoma, Cell Signaling Technology Hspa1b antibody (Cell signaling, 4872) was used in flow cytometry on human samples at 1:25 (fig 1b), in immunohistochemistry on human samples (fig 1a) and in western blot on human samples at 1:500 (fig 1d). Chem Biol Interact (2016) ncbi
Articles Reviewed
  1. Mahameed M, Boukeileh S, Obiedat A, Darawshi O, Dipta P, Rimon A, et al. Pharmacological induction of selective endoplasmic reticulum retention as a strategy for cancer therapy. Nat Commun. 2020;11:1304 pubmed publisher
  2. Gaglia G, Rashid R, Yapp C, Joshi G, Li C, Lindquist S, et al. HSF1 phase transition mediates stress adaptation and cell fate decisions. Nat Cell Biol. 2020;22:151-158 pubmed publisher
  3. Yang X, Yang J, Lei P, Wen T. LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis. Aging (Albany NY). 2019;11:8777-8791 pubmed publisher
  4. Zhang Y, Jin X, Liang J, Guo Y, Sun G, Zeng X, et al. Extracellular vesicles derived from ODN-stimulated macrophages transfer and activate Cdc42 in recipient cells and thereby increase cellular permissiveness to EV uptake. Sci Adv. 2019;5:eaav1564 pubmed publisher
  5. Wegmann S, Bennett R, Delorme L, Robbins A, Hu M, McKenzie D, et al. Experimental evidence for the age dependence of tau protein spread in the brain. Sci Adv. 2019;5:eaaw6404 pubmed publisher
  6. Stewart E, McEvoy J, Wang H, Chen X, Honnell V, Ocarz M, et al. Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses. Cancer Cell. 2018;34:411-426.e19 pubmed publisher
  7. Zhang X, Zhuang R, Wu H, Chen J, Wang F, Li G, et al. A novel role of endocan in alleviating LPS-induced acute lung injury. Life Sci. 2018;202:89-97 pubmed publisher
  8. Savitski M, Zinn N, Faelth Savitski M, Poeckel D, Gade S, Becher I, et al. Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis. Cell. 2018;173:260-274.e25 pubmed publisher
  9. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  10. Gasparotto J, Girardi C, Somensi N, Ribeiro C, Moreira J, Michels M, et al. Receptor for advanced glycation end products mediates sepsis-triggered amyloid-β accumulation, Tau phosphorylation, and cognitive impairment. J Biol Chem. 2018;293:226-244 pubmed publisher
  11. Zhao T, Hong Y, Yin P, Li S, Li X. Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins. Proc Natl Acad Sci U S A. 2017;114:E7803-E7811 pubmed publisher
  12. Aprile F, Källstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C. The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress α-synuclein aggregation. Sci Rep. 2017;7:9039 pubmed publisher
  13. Qin L, Tan J, Zhang H, Rizwana K, Lu J, Tang J, et al. BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage. Oxid Med Cell Longev. 2017;2017:5094934 pubmed publisher
  14. Gupta A, Anjomani Virmouni S, Koundouros N, Dimitriadi M, Choo Wing R, Valle A, et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell. 2017;65:999-1013.e7 pubmed publisher
  15. Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gandia J, et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol. 2017;13:918 pubmed publisher
  16. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  17. Kattaia A, Abd El Baset S, Mohamed E, Abdul Maksou R, Elfakharany Y. Molecular mechanisms underlying histological and biochemical changes induced by nitrate in rat liver and the efficacy of S-Allylcysteine. Ultrastruct Pathol. 2017;41:10-22 pubmed publisher
  18. Cheng Y, Huang C, Lee Y, Tien L, Ku W, Chien R, et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep. 2016;6:30314 pubmed publisher
  19. Wang C, Guo S, Wang J, Yan X, Farrelly M, Zhang Y, et al. Reactivation of ERK and Akt confers resistance of mutant BRAF colon cancer cells to the HSP90 inhibitor AUY922. Oncotarget. 2016;7:49597-49610 pubmed publisher
  20. Guo Y, Cui L, Jiang S, Wang D, Jiang S, Xie C, et al. S100A1 transgenic treatment of acute heart failure causes proteomic changes in rats. Mol Med Rep. 2016;14:1538-52 pubmed publisher
  21. Trousil S, Kaliszczak M, Schug Z, Nguyen Q, Tomasi G, Favicchio R, et al. The novel choline kinase inhibitor ICL-CCIC-0019 reprograms cellular metabolism and inhibits cancer cell growth. Oncotarget. 2016;7:37103-37120 pubmed publisher
  22. Venkatesan N, Kanwar J, Deepa P, Navaneethakrishnan S, Joseph C, Krishnakumar S. Targeting HSP90/Survivin using a cell permeable structure based peptido-mimetic shepherdin in retinoblastoma. Chem Biol Interact. 2016;252:141-9 pubmed publisher
  23. Kong F, Wang H, Guo J, Peng M, Ji H, Yang H, et al. Hsp70 suppresses apoptosis of BRL cells by regulating the expression of Bcl-2, cytochrome C, and caspase 8/3. In Vitro Cell Dev Biol Anim. 2016;52:568-75 pubmed publisher
  24. Peng C, Kaščáková S, Chiappini F, Olaya N, Sandt C, Yousef I, et al. Discrimination of cirrhotic nodules, dysplastic lesions and hepatocellular carcinoma by their vibrational signature. J Transl Med. 2016;14:9 pubmed publisher
  25. Roundhill E, Turnbull D, Burchill S. Localization of MRP-1 to the outer mitochondrial membrane by the chaperone protein HSP90β. FASEB J. 2016;30:1712-23 pubmed publisher
  26. Tang S, Chen H, Cheng Y, Nasir M, Kemper N, Bao E. The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress. Int J Mol Med. 2016;37:56-62 pubmed publisher
  27. Toni L, Padilla P. Developmentally arrested Austrofundulus limnaeus embryos have changes in post-translational modifications of histone H3. J Exp Biol. 2016;219:544-52 pubmed publisher
  28. Yeung H, Lo P, Ng D, Fong W. Anti-tumor immunity of BAM-SiPc-mediated vascular photodynamic therapy in a BALB/c mouse model. Cell Mol Immunol. 2017;14:223-234 pubmed publisher
  29. Nuss J, Kehn Hall K, Benedict A, Costantino J, Ward M, Peyser B, et al. Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors. PLoS ONE. 2014;9:e93483 pubmed publisher
  30. Qi L, Zhang X, Wu J, Lin F, Wang J, DiFiglia M, et al. The role of chaperone-mediated autophagy in huntingtin degradation. PLoS ONE. 2012;7:e46834 pubmed publisher