This is a Validated Antibody Database (VAD) review about mouse Hist1h2af, based on 13 published articles (read how Labome selects the articles), using Hist1h2af antibody in all methods. It is aimed to help Labome visitors find the most suited Hist1h2af antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Hist1h2af synonym: H2a-221; histone H2A type 1-F; histone 1, H2af; histone H2a (aa 92-128)

Cell Signaling Technology
mouse monoclonal (L88A6)
  • western blot; human; loading ...; fig 1c
In order to study the role of ACK1/TNK2 in histone H4 Tyr88-phosphorylation and androgen receptor expression in prostate cancer, Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 3636) was used in western blot on human samples (fig 1c). Cancer Cell (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig s4
Cell Signaling Technology Hist1h2af antibody (cell signalling, 2578) was used in western blot on mouse samples (fig s4). J Cell Sci (2017) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4c
Cell Signaling Technology Hist1h2af antibody (cell signalling, 2576P) was used in western blot on mouse samples (fig 4c). J Clin Invest (2017) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4c
Cell Signaling Technology Hist1h2af antibody (cell signalling, 2578P) was used in western blot on mouse samples (fig 4c). J Clin Invest (2017) ncbi
mouse monoclonal (L88A6)
  • western blot; human; loading ...
In order to investigate the role of FACT in sensing DNA torsional stress, Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 3636S) was used in western blot on human samples . Nucleic Acids Res (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 2578) was used in western blot on mouse samples at 1:1000 (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (L88A6)
  • western blot; mouse; fig 4b
Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 3636s) was used in western blot on mouse samples (fig 4b). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; rat; fig 5
  • western blot; rat; fig 5
Cell Signaling Technology Hist1h2af antibody (cell Signaling, 2576) was used in chromatin immunoprecipitation on rat samples (fig 5) and in western blot on rat samples (fig 5). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4a
In order to elucidate the function of TRIM29 in double stranded break repair, Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 2,578) was used in western blot on human samples at 1:1000 (fig 4a). Nat Commun (2015) ncbi
rabbit polyclonal
  • flow cytometry; human
In order to elucidate the function of TRIM29 in double stranded break repair, Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 2,576) was used in flow cytometry on human samples . Nat Commun (2015) ncbi
mouse monoclonal (L88A6)
  • western blot; human; 1:1000
Cell Signaling Technology Hist1h2af antibody (Cell Signaling, 3636) was used in western blot on human samples at 1:1000. Endocrinology (2015) ncbi
rabbit polyclonal
  • immunoprecipitation; human
In order to study the interaction between BRCA1 tumor suppressor and inositol 1,4,5-trisphosphate receptors and its effect on apoptotic calcium release, Cell Signaling Technology Hist1h2af antibody (Cell Signaling Technology, 2576) was used in immunoprecipitation on human samples . J Biol Chem (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; fig s2
Cell Signaling Technology Hist1h2af antibody (Cell Signalling, 2578) was used in immunohistochemistry on human samples (fig s2). Aging Cell (2015) ncbi
mouse monoclonal (L88A6)
  • western blot; human; fig 1
Cell Signaling Technology Hist1h2af antibody (Cell Signaling Technology, L88A6) was used in western blot on human samples (fig 1). Mol Cell Biol (2015) ncbi
mouse monoclonal (L88A6)
  • western blot; human
Cell Signaling Technology Hist1h2af antibody (Cell Signaling Technology, 3636) was used in western blot on human samples . J Biol Chem (2014) ncbi
Articles Reviewed
  1. Mahajan K, Malla P, Lawrence H, Chen Z, Kumar Sinha C, Malik R, et al. ACK1/TNK2 Regulates Histone H4 Tyr88-phosphorylation and AR Gene Expression in Castration-Resistant Prostate Cancer. Cancer Cell. 2017;31:790-803.e8 pubmed publisher
  2. Jha K, Tripurani S, Johnson G. TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis. J Cell Sci. 2017;130:1835-1844 pubmed publisher
  3. Wu N, Jia D, Bates B, Basom R, Eberhart C, MacPherson D. A mouse model of MYCN-driven retinoblastoma reveals MYCN-independent tumor reemergence. J Clin Invest. 2017;127:888-898 pubmed publisher
  4. Safina A, Cheney P, Pal M, Brodsky L, Ivanov A, Kirsanov K, et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res. 2017;45:1925-1945 pubmed publisher
  5. Baleriola J, Álvarez Lindo N, de la Villa P, Bernad A, Blanco L, Suárez T, et al. Increased neuronal death and disturbed axonal growth in the Polμ-deficient mouse embryonic retina. Sci Rep. 2016;6:25928 pubmed publisher
  6. Ramasamy S, Saez B, Mukhopadhyay S, Ding D, Ahmed A, Chen X, et al. Tle1 tumor suppressor negatively regulates inflammation in vivo and modulates NF-κB inflammatory pathway. Proc Natl Acad Sci U S A. 2016;113:1871-6 pubmed publisher
  7. Namachivayam K, Mohankumar K, Arbach D, Jagadeeswaran R, Jain S, Natarajan V, et al. All-Trans Retinoic Acid Induces TGF-β2 in Intestinal Epithelial Cells via RhoA- and p38α MAPK-Mediated Activation of the Transcription Factor ATF2. PLoS ONE. 2015;10:e0134003 pubmed publisher
  8. Masuda Y, Takahashi H, Sato S, Tomomori Sato C, Saraf A, Washburn M, et al. TRIM29 regulates the assembly of DNA repair proteins into damaged chromatin. Nat Commun. 2015;6:7299 pubmed publisher
  9. Zhong X, Liao Y, Chen L, Liu G, Feng Y, Zeng T, et al. The MicroRNAs in the Pathogenesis of Metabolic Memory. Endocrinology. 2015;156:3157-68 pubmed publisher
  10. Hedgepeth S, Garcia M, Wagner L, Rodriguez A, Chintapalli S, Snyder R, et al. The BRCA1 tumor suppressor binds to inositol 1,4,5-trisphosphate receptors to stimulate apoptotic calcium release. J Biol Chem. 2015;290:7304-13 pubmed publisher
  11. Gibbs Seymour I, Markiewicz E, Bekker Jensen S, Mailand N, Hutchison C. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage. Aging Cell. 2015;14:162-9 pubmed publisher
  12. Lecona E, Narendra V, Reinberg D. USP7 cooperates with SCML2 to regulate the activity of PRC1. Mol Cell Biol. 2015;35:1157-68 pubmed publisher
  13. Hock A, Vigneron A, Vousden K. Ubiquitin-specific peptidase 42 (USP42) functions to deubiquitylate histones and regulate transcriptional activity. J Biol Chem. 2014;289:34862-70 pubmed publisher