This is a Validated Antibody Database (VAD) review about mouse Gli2, based on 21 published articles (read how Labome selects the articles), using Gli2 antibody in all methods. It is aimed to help Labome visitors find the most suited Gli2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Gli2 synonym: zinc finger protein GLI2; Tax helper protein

Knockout validation
R&D Systems
goat polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s2a
In order to analyze the contributions of developmental Hedgehog signaling effectors HDAC1 and HDAC2 in SHH Medulloblastoma, R&D Systems Gli2 antibody (R&D, AF3635) was used in western blot knockout validation on mouse samples at 1:1000 (fig s2a). Sci Rep (2017) ncbi
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 4a
Proteintech Group Gli2 antibody (Proteintech, 18989) was used in western blot knockout validation on human samples (fig 4a). elife (2019) ncbi
Abcam
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; fig 8
Abcam Gli2 antibody (abcam, ab167389) was used in immunocytochemistry on mouse samples at 1:100 (fig 8). Nat Commun (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 5
  • western blot; human; fig s5
Abcam Gli2 antibody (Abcam, ab26056) was used in chromatin immunoprecipitation on human samples (fig 5) and in western blot on human samples (fig s5). Cell Death Dis (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100
Abcam Gli2 antibody (Abcam, ab26056) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Cell Death Dis (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Abcam Gli2 antibody (Abcam, ab26056) was used in western blot on human samples (fig 5). Cell Signal (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100
Abcam Gli2 antibody (Abcam, AB26056) was used in immunocytochemistry on mouse samples at 1:100. Toxicol Appl Pharmacol (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; human; fig 3
  • western blot; human; fig 2
In order to elucidate the contribution of lncRNA BCAR4 in breast cancer metastasis, Abcam Gli2 antibody (Abcam, ab26056) was used in immunoprecipitation on human samples (fig 3) and in western blot on human samples (fig 2). Cell (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 2
  • western blot; human; 1:1000; fig 2
In order to report a regulatory role for the Ptc1-Gli1 complex in adult neural circuit integrity of the brain, Abcam Gli2 antibody (Abcam, ab26056) was used in western blot on mouse samples at 1:1000 (fig 2) and in western blot on human samples at 1:1000 (fig 2). Hum Mol Genet (2014) ncbi
rabbit polyclonal
  • western blot; fruit fly
Abcam Gli2 antibody (Abcam, ab26056) was used in western blot on fruit fly samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam Gli2 antibody (Abcam, ab26056) was used in western blot on human samples . Biol Pharm Bull (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Abcam Gli2 antibody (Abcam, ab26056) was used in western blot on mouse samples at 1:1000. Differentiation (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
  • western blot; human
Abcam Gli2 antibody (Abcam, ab26056) was used in immunocytochemistry on human samples and in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200
Abcam Gli2 antibody (Abcam, ab26056) was used in immunocytochemistry on mouse samples at 1:200. Genomics (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200
Abcam Gli2 antibody (Abcam, ab26056) was used in immunocytochemistry on human samples at 1:200. Oncogene (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human
Abcam Gli2 antibody (Abcam, Ab26056) was used in immunohistochemistry - paraffin section on human samples . Am J Pathol (2012) ncbi
R&D Systems
goat polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s2a
In order to analyze the contributions of developmental Hedgehog signaling effectors HDAC1 and HDAC2 in SHH Medulloblastoma, R&D Systems Gli2 antibody (R&D, AF3635) was used in western blot knockout validation on mouse samples at 1:1000 (fig s2a). Sci Rep (2017) ncbi
goat polyclonal
  • western blot; mouse; 1:500; fig 4c
R&D Systems Gli2 antibody (R&D Systems, AF3635) was used in western blot on mouse samples at 1:500 (fig 4c). Proc Natl Acad Sci U S A (2016) ncbi
goat polyclonal
  • western blot; mouse; 1:1000; fig 3
R&D Systems Gli2 antibody (R&D Systems, AF3635) was used in western blot on mouse samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi
goat polyclonal
R&D Systems Gli2 antibody (R&D Systems, AF3635) was used . J Clin Invest (2015) ncbi
goat polyclonal
  • immunocytochemistry; mouse; 1:500
  • western blot; mouse; 1:1000
In order to study the effect of Gli2 ciliary localization on Hedgehog signaling, R&D Systems Gli2 antibody (R&D Systems, AF3635) was used in immunocytochemistry on mouse samples at 1:500 and in western blot on mouse samples at 1:1000. Development (2015) ncbi
goat polyclonal
  • western blot; mouse
R&D Systems Gli2 antibody (R&D Systems, AF3635) was used in western blot on mouse samples . J Clin Invest (2014) ncbi
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 4a
Proteintech Group Gli2 antibody (Proteintech, 18989) was used in western blot knockout validation on human samples (fig 4a). elife (2019) ncbi
Articles Reviewed
  1. Adams C, Htwe H, Marsh T, Wang A, Montoya M, Subbaraj L, et al. Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer. elife. 2019;8: pubmed publisher
  2. Coni S, Mancuso A, Di Magno L, Sdruscia G, Manni S, Serrao S, et al. Selective targeting of HDAC1/2 elicits anticancer effects through Gli1 acetylation in preclinical models of SHH Medulloblastoma. Sci Rep. 2017;7:44079 pubmed publisher
  3. Cai H, Liu A. Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling. Proc Natl Acad Sci U S A. 2016;113:14751-14756 pubmed publisher
  4. Torrado B, Grana M, Badano J, Irigoín F. Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-α/β1-Independent. PLoS ONE. 2016;11:e0162033 pubmed publisher
  5. Yan B, Zhang Z, Jin D, Cai C, Jia C, Liu W, et al. mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation. Nat Commun. 2016;7:11151 pubmed publisher
  6. Vlčková K, Ondrušová L, Vachtenheim J, Réda J, Dundr P, Zadinová M, et al. Survivin, a novel target of the Hedgehog/GLI signaling pathway in human tumor cells. Cell Death Dis. 2016;7:e2048 pubmed publisher
  7. Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, et al. Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis. J Clin Invest. 2015;125:2935-51 pubmed publisher
  8. Liu J, Zeng H, Liu A. The loss of Hh responsiveness by a non-ciliary Gli2 variant. Development. 2015;142:1651-60 pubmed publisher
  9. Pan Y, Gong Y, Ruan H, Pan L, Wu X, Tang C, et al. Sonic hedgehog through Gli2 and Gli3 is required for the proper development of placental labyrinth. Cell Death Dis. 2015;6:e1653 pubmed publisher
  10. Tang C, Pan Y, Luo H, Xiong W, Zhu H, Ruan H, et al. Hedgehog signaling stimulates the conversion of cholesterol to steroids. Cell Signal. 2015;27:487-97 pubmed publisher
  11. Liu J, Bain L. Arsenic inhibits hedgehog signaling during P19 cell differentiation. Toxicol Appl Pharmacol. 2014;281:243-53 pubmed publisher
  12. Xing Z, Lin A, Li C, Liang K, Wang S, Liu Y, et al. lncRNA directs cooperative epigenetic regulation downstream of chemokine signals. Cell. 2014;159:1110-1125 pubmed publisher
  13. He P, Staufenbiel M, Li R, Shen Y. Deficiency of patched 1-induced Gli1 signal transduction results in astrogenesis in Swedish mutated APP transgenic mice. Hum Mol Genet. 2014;23:6512-27 pubmed publisher
  14. Shahi M, Holt R, Rebhun R. Blocking signaling at the level of GLI regulates downstream gene expression and inhibits proliferation of canine osteosarcoma cells. PLoS ONE. 2014;9:e96593 pubmed publisher
  15. Matsumoto T, Tabata K, Suzuki T. The GANT61, a GLI inhibitor, induces caspase-independent apoptosis of SK-N-LO cells. Biol Pharm Bull. 2014;37:633-41 pubmed
  16. Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, et al. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. J Clin Invest. 2014;124:2059-70 pubmed
  17. Romani S, Illi B, De Mori R, Savino M, Gleeson J, Valente E. The ciliary proteins Meckelin and Jouberin are required for retinoic acid-dependent neural differentiation of mouse embryonic stem cells. Differentiation. 2014;87:134-146 pubmed publisher
  18. Chen Q, Xu R, Zeng C, Lu Q, Huang D, Shi C, et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ?4-mediated FAK signaling. PLoS ONE. 2014;9:e88386 pubmed publisher
  19. Li Y, Drnevich J, Akraiko T, Band M, Li D, Wang F, et al. Gene expression profiling reveals the heterogeneous transcriptional activity of Oct3/4 and its possible interaction with Gli2 in mouse embryonic stem cells. Genomics. 2013;102:456-67 pubmed publisher
  20. Bosco Clément G, Zhang F, Chen Z, Zhou H, Li H, Mikami I, et al. Targeting Gli transcription activation by small molecule suppresses tumor growth. Oncogene. 2014;33:2087-97 pubmed publisher
  21. Cigna N, Farrokhi Moshai E, Brayer S, Marchal Sommé J, Wemeau Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-?-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol. 2012;181:2126-37 pubmed publisher