This is a Validated Antibody Database (VAD) review about mouse Gja5, based on 25 published articles (read how Labome selects the articles), using Gja5 antibody in all methods. It is aimed to help Labome visitors find the most suited Gja5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Gja5 synonym: 5730555N10Rik; Cnx40; Cx40; Gja-5; gap junction alpha-5 protein; connexin-40; gap junction membrane channel protein alpha 5

Invitrogen
rabbit polyclonal
  • immunohistochemistry; mouse; 1:300; loading ...; fig s8
Invitrogen Gja5 antibody (Invitrogen, 36-4900) was used in immunohistochemistry on mouse samples at 1:300 (fig s8). Nat Commun (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2a
  • western blot; mouse; 1:1000; loading ...; fig 4a
In order to examine connexin40 and connexin43 expression in mouse models of hypoxia and sleep apnea, Invitrogen Gja5 antibody (Life Technologies, 36-4900) was used in immunohistochemistry on mouse samples at 1:200 (fig 2a) and in western blot on mouse samples at 1:1000 (fig 4a). BMC Cell Biol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1b
In order to determine the role of mitochondrial Cx40 in endothelial cells, Invitrogen Gja5 antibody (Thermo, 36-4900) was used in immunocytochemistry on mouse samples at 1:200 (fig 1b). Am J Physiol Cell Physiol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 4a
In order to characterize residues important for connexin pore formation, Invitrogen Gja5 antibody (ThermoFisher, 36-4900) was used in immunocytochemistry on human samples at 1:200 (fig 4a). Channels (Austin) (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig s11
In order to determine the autonomouse beating rate adaptation in cardiomyocytes from human stem cells, Invitrogen Gja5 antibody (Life Technologies, 36-4900) was used in immunocytochemistry on human samples at 1:100 (fig s11). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
In order to study the effect of G9a histone methyltransferase inhibitor on bone marrow mesenchymal stem cells, Invitrogen Gja5 antibody (Life Technologies, 36-5000) was used in immunocytochemistry on mouse samples . Stem Cells Int (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to test if H2S supplementation reduces MMP-9-induced extracellular matrix remodeling and dysfunction in diabetic kidney, Invitrogen Gja5 antibody (Life Technologies, 6-4900) was used in western blot on mouse samples . Nitric Oxide (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:250; fig 3
In order to study Cx40 mutants and determine how they contribute to atrial fibrillation pathogenesis, Invitrogen Gja5 antibody (Life Technologies, 36-4900) was used in western blot on human samples at 1:250 (fig 3). J Mol Cell Cardiol (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig s7
In order to demonstrate that HACE1 protects the heart under pressure stress by regulating protein degradation, Invitrogen Gja5 antibody (Life Technologies, 36-5000) was used in western blot on mouse samples at 1:1000 (fig s7). Nat Commun (2014) ncbi
mouse monoclonal (2F9A11)
  • immunocytochemistry; mouse
  • immunocytochemistry; rat
In order to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus, Invitrogen Gja5 antibody (Life Technologies, 37-8900) was used in immunocytochemistry on mouse samples and in immunocytochemistry on rat samples . Eur J Neurosci (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; rat
  • immunocytochemistry; mouse
In order to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus, Invitrogen Gja5 antibody (Life Technologies, 36-5000) was used in immunocytochemistry on rat samples and in immunocytochemistry on mouse samples . Eur J Neurosci (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:200; fig 7
In order to study two autosomal recessive mutations reported that cause oculodentodigital dysplasia, Invitrogen Gja5 antibody (Invitrogen, 36-4900) was used in western blot on human samples at 1:200 (fig 7). J Cell Sci (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; fig 8
In order to examine the vulnerability of different cardiac gap junction channels formed of connexins 43, 40, and 45 to simulated ischemia, Invitrogen Gja5 antibody (Invitrogen, 36-5000) was used in immunocytochemistry on mouse samples (fig 8). PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; mouse; fig 8
In order to test if low density lipoprotein cholesterol affects calcium dynamics and signal propagation in cultured atrial myocytes, Invitrogen Gja5 antibody (Invitrogen, 36-4900) was used in western blot on mouse samples (fig 8). PLoS ONE (2013) ncbi
mouse monoclonal (2F9A11)
  • immunohistochemistry; mouse; 1:200; fig 4
In order to examine the role of connexin 40 in the endothelial Ca(2+) signaling of the mouse aorta, Invitrogen Gja5 antibody (Invitrogen, 37-8900) was used in immunohistochemistry on mouse samples at 1:200 (fig 4). Cell Physiol Biochem (2013) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; fig 5
In order to test if TBX5 regulates transcriptional networks required for mature cardiac conduction system function, Invitrogen Gja5 antibody (Zymed, 36-4900) was used in immunohistochemistry on mouse samples at 1:250 (fig 5). J Clin Invest (2012) ncbi
mouse monoclonal (2F9A11)
  • immunoprecipitation; rat; fig 5
  • immunocytochemistry; rat; 1:250; fig 5
  • western blot; rat; 1:250; fig 5
In order to study the involvement of gap junctions in calcium wave propagation between smooth muscle cells, Invitrogen Gja5 antibody (Invitrogen, 37-8900) was used in immunoprecipitation on rat samples (fig 5), in immunocytochemistry on rat samples at 1:250 (fig 5) and in western blot on rat samples at 1:250 (fig 5). Cell Commun Adhes (2012) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; fig 3
In order to assess how chronic pressure overload affects the Purkinje fibers of the ventricular peripheral conduction system, Invitrogen Gja5 antibody (Invitrogen, 36-4900) was used in immunohistochemistry on mouse samples (fig 3). Am J Physiol Heart Circ Physiol (2012) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; fig 3
In order to use transgenic mice to study the cardiac conduction system, Invitrogen Gja5 antibody (Zymed, 36-4900) was used in immunohistochemistry on mouse samples at 1:250 (fig 3). Genesis (2011) ncbi
rabbit polyclonal
  • western blot; dog; fig 2
In order to report that TBX18 represses Cx43 transcript and protein levels in neonatal rat cardiomyocytes, Invitrogen Gja5 antibody (Invitrogen, 36-4900) was used in western blot on dog samples (fig 2). J Biol Chem (2011) ncbi
mouse monoclonal (2F9A11)
  • immunohistochemistry; mouse; 1:200; fig 5
In order to explore the link between the expression of endothelial connexins and nitric oxide synthase expression and function in the mouse aorta, Invitrogen Gja5 antibody (Invitrogen, 37?C8900) was used in immunohistochemistry on mouse samples at 1:200 (fig 5). Am J Physiol Heart Circ Physiol (2010) ncbi
EMD Millipore
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig S1
EMD Millipore Gja5 antibody (Merck Millipore, AB1726) was used in immunohistochemistry on mouse samples at 1:100 (fig S1). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
EMD Millipore Gja5 antibody (Millipore, AB1726) was used in western blot on mouse samples at 1:1000. J Mol Cell Cardiol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:200
EMD Millipore Gja5 antibody (Chemicon International, AB1726) was used in immunohistochemistry on rat samples at 1:200. Br J Pharmacol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
EMD Millipore Gja5 antibody (Millipore, ab1726) was used in western blot on mouse samples at 1:1000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; rabbit; 1:500
In order to study the expression of connexin 57 in rabbit retina B-type horizontal cells, EMD Millipore Gja5 antibody (Chemicon / Millipore, AB1726) was used in immunohistochemistry on rabbit samples at 1:500. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. Fang J, Coon B, Gillis N, Chen Z, Qiu J, Chittenden T, et al. Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. Nat Commun. 2017;8:2149 pubmed publisher
  2. Gemel J, Su Z, Gileles Hillel A, Khalyfa A, Gozal D, Beyer E. Intermittent hypoxia causes NOX2-dependent remodeling of atrial connexins. BMC Cell Biol. 2017;18:7 pubmed publisher
  3. Guo R, Si R, Scott B, Makino A. Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells. Am J Physiol Cell Physiol. 2017;312:C398-C406 pubmed publisher
  4. Xu Q, Lin X, Matiukas A, Zhang X, Veenstra R. Specificity of the connexin W3/4 locus for functional gap junction formation. Channels (Austin). 2016;10:453-65 pubmed publisher
  5. Kanda M, Nagai T, Takahashi T, Liu M, Kondou N, Naito A, et al. Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction. PLoS ONE. 2016;11:e0156562 pubmed publisher
  6. Eng G, Lee B, Protas L, Gagliardi M, Brown K, Kass R, et al. Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. Nat Commun. 2016;7:10312 pubmed publisher
  7. Meraviglia V, Azzimato V, Colussi C, Florio M, Binda A, Panariti A, et al. Acetylation mediates Cx43 reduction caused by electrical stimulation. J Mol Cell Cardiol. 2015;87:54-64 pubmed publisher
  8. Yang J, Kaur K, Ong L, Eisenberg C, Eisenberg L. Inhibition of G9a Histone Methyltransferase Converts Bone Marrow Mesenchymal Stem Cells to Cardiac Competent Progenitors. Stem Cells Int. 2015;2015:270428 pubmed publisher
  9. Kundu S, Pushpakumar S, Sen U. MMP-9- and NMDA receptor-mediated mechanism of diabetic renovascular remodeling and kidney dysfunction: hydrogen sulfide is a key modulator. Nitric Oxide. 2015;46:172-85 pubmed publisher
  10. Garland C, Smirnov S, Bagher P, Lim C, Huang C, Mitchell R, et al. TRPM4 inhibitor 9-phenanthrol activates endothelial cell intermediate conductance calcium-activated potassium channels in rat isolated mesenteric artery. Br J Pharmacol. 2015;172:1114-23 pubmed publisher
  11. Gemel J, Simon A, Patel D, Xu Q, Matiukas A, Veenstra R, et al. Degradation of a connexin40 mutant linked to atrial fibrillation is accelerated. J Mol Cell Cardiol. 2014;74:330-9 pubmed publisher
  12. Swärd K, Albinsson S, Rippe C. Arterial dysfunction but maintained systemic blood pressure in cavin-1-deficient mice. PLoS ONE. 2014;9:e92428 pubmed publisher
  13. Zhang L, Chen X, Sharma P, Moon M, Sheftel A, Dawood F, et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat Commun. 2014;5:3430 pubmed publisher
  14. Bautista W, Rash J, Vanderpool K, Yasumura T, Nagy J. Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus. Eur J Neurosci. 2014;39:757-70 pubmed publisher
  15. Huang T, Shao Q, Macdonald A, Xin L, Lorentz R, Bai D, et al. Autosomal recessive GJA1 (Cx43) gene mutations cause oculodentodigital dysplasia by distinct mechanisms. J Cell Sci. 2013;126:2857-66 pubmed publisher
  16. Sahu G, Bera A. Contribution of intracellular calcium and pH in ischemic uncoupling of cardiac gap junction channels formed of connexins 43, 40, and 45: a critical function of C-terminal domain. PLoS ONE. 2013;8:e60506 pubmed publisher
  17. Barriga M, Cal R, Cabello N, Llach A, Vallmitjana A, Benitez R, et al. Low density lipoproteins promote unstable calcium handling accompanied by reduced SERCA2 and connexin-40 expression in cardiomyocytes. PLoS ONE. 2013;8:e58128 pubmed publisher
  18. Boittin F, Alonso F, Le Gal L, Allagnat F, Beny J, Haefliger J. Connexins and M3 muscarinic receptors contribute to heterogeneous Ca(2+) signaling in mouse aortic endothelium. Cell Physiol Biochem. 2013;31:166-78 pubmed publisher
  19. Arnolds D, Liu F, Fahrenbach J, Kim G, Schillinger K, Smemo S, et al. TBX5 drives Scn5a expression to regulate cardiac conduction system function. J Clin Invest. 2012;122:2509-18 pubmed publisher
  20. Halidi N, Alonso F, Burt J, Beny J, Haefliger J, Meister J. Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43. Cell Commun Adhes. 2012;19:25-37 pubmed publisher
  21. Pan F, Keung J, Kim I, Snuggs M, Mills S, O BRIEN J, et al. Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina. J Comp Neurol. 2012;520:2256-74 pubmed publisher
  22. Harris B, Baicu C, Haghshenas N, Kasiganesan H, Scholz D, Rackley M, et al. Remodeling of the peripheral cardiac conduction system in response to pressure overload. Am J Physiol Heart Circ Physiol. 2012;302:H1712-25 pubmed publisher
  23. Arnolds D, Moskowitz I. Inducible recombination in the cardiac conduction system of minK: CreERT² BAC transgenic mice. Genesis. 2011;49:878-84 pubmed publisher
  24. Kapoor N, Galang G, Marban E, Cho H. Transcriptional suppression of connexin43 by TBX18 undermines cell-cell electrical coupling in postnatal cardiomyocytes. J Biol Chem. 2011;286:14073-9 pubmed publisher
  25. Alonso F, Boittin F, Beny J, Haefliger J. Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta. Am J Physiol Heart Circ Physiol. 2010;299:H1365-73 pubmed publisher