This is a Validated Antibody Database (VAD) review about mouse G9a, based on 29 published articles (read how Labome selects the articles), using G9a antibody in all methods. It is aimed to help Labome visitors find the most suited G9a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
G9a synonym: Bat8; D17Ertd710e; G9a; KMT1C; NG36

Abcam
domestic rabbit monoclonal (EPR18894)
  • western blot; mouse; 1:1000; fig 1a
Abcam G9a antibody (Abcam, ab185050) was used in western blot on mouse samples at 1:1000 (fig 1a). elife (2022) ncbi
domestic rabbit monoclonal (EPR18894)
  • western blot; human; 1:2000; loading ...; fig 3c
Abcam G9a antibody (Abcam, ab185050) was used in western blot on human samples at 1:2000 (fig 3c). Aging (Albany NY) (2020) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; human; fig 3
Abcam G9a antibody (Abcam, ab40542) was used in chromatin immunoprecipitation on mouse samples (fig 2) and in immunoprecipitation on human samples (fig 3). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; 1:40; loading ...; fig 3b
In order to propose that the NKX3.1-G9a-UTY transcriptional regulatory network is essential for prostate differentiation, Abcam G9a antibody (Abcam, ab40542) was used in chromatin immunoprecipitation on human samples at 1:40 (fig 3b). Science (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4
  • immunoprecipitation; human; fig 5
  • western blot; human; fig 5
Abcam G9a antibody (Abcam, ab40542) was used in chromatin immunoprecipitation on human samples (fig 4), in immunoprecipitation on human samples (fig 5) and in western blot on human samples (fig 5). Nucleic Acids Res (2016) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 5
Abcam G9a antibody (Abcam, ab40542) was used in chromatin immunoprecipitation on human samples (fig 5). Nat Struct Mol Biol (2016) ncbi
Invitrogen
domestic rabbit monoclonal (B.133.9)
  • western blot; human; loading ...; fig 2c
In order to study how methylation of p53 affects its interactions with other proteins such as PUMA and p300/CBP, Invitrogen G9a antibody (Thermo Fisher, MA5-14880) was used in western blot on human samples (fig 2c). Oncogene (2017) ncbi
domestic rabbit monoclonal (B.133.9)
  • western blot; human; fig 3
In order to assess the necessity of S-adenosyl methionine for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3, Invitrogen G9a antibody (Thermo Fisher, MA5-14880) was used in western blot on human samples (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C6H3)
  • immunocytochemistry; human; 1:1000; fig 4c
  • western blot; human; 1:500; fig 4b
Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in immunocytochemistry on human samples at 1:1000 (fig 4c) and in western blot on human samples at 1:500 (fig 4b). Front Cell Dev Biol (2021) ncbi
domestic rabbit monoclonal (C6H3)
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology G9a antibody (Cell Signalling, 3306S) was used in immunohistochemistry on human samples (fig 1a) and in western blot on human samples (fig 2a). elife (2020) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology G9a antibody (Cell Signaling Technology, 3306) was used in western blot on mouse samples (fig 5a). Cancer Res (2019) ncbi
domestic rabbit monoclonal (C6H3)
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3b
  • flow cytometry; mouse; loading ...; fig s3b
Cell Signaling Technology G9a antibody (Cell Signaling, C6H3) was used in immunohistochemistry - frozen section on mouse samples (fig s3b) and in flow cytometry on mouse samples (fig s3b). J Cell Biol (2018) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; human; 1:1000; loading ...; fig 1a
Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in western blot on human samples at 1:1000 (fig 1a). Nat Commun (2017) ncbi
domestic rabbit monoclonal (C6H3)
  • immunohistochemistry - paraffin section; pigs ; 1:100; loading ...; fig s3b
In order to elucidate the inital stage of developmental events from human primordial germ cells, Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in immunohistochemistry - paraffin section on pigs samples at 1:100 (fig s3b). Nature (2017) ncbi
domestic rabbit monoclonal (C6H3)
  • immunoprecipitation; human; 1:1000; loading ...; fig 4g
  • western blot; human; 1:1000; loading ...; fig 4f
Cell Signaling Technology G9a antibody (Cell Signaling Technology, 3306S) was used in immunoprecipitation on human samples at 1:1000 (fig 4g) and in western blot on human samples at 1:1000 (fig 4f). Mol Cell (2017) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; mouse; fig 2
  • western blot; human; fig 3
Cell Signaling Technology G9a antibody (Cell Signaling, 3306S) was used in western blot on mouse samples (fig 2) and in western blot on human samples (fig 3). Sci Rep (2016) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; zebrafish ; fig 1
In order to report that ZNF644 is a co-regulator of G9a/H3K9me2-mediated gene silencing during neuronal differentiation, Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in western blot on zebrafish samples (fig 1). Stem Cell Reports (2016) ncbi
domestic rabbit monoclonal (C6H3)
  • immunoprecipitation; mouse; fig 6
  • immunocytochemistry; mouse; fig 6
  • western blot; mouse; fig 1
Cell Signaling Technology G9a antibody (Cell Signaling, 3306S) was used in immunoprecipitation on mouse samples (fig 6), in immunocytochemistry on mouse samples (fig 6) and in western blot on mouse samples (fig 1). Nucleic Acids Res (2016) ncbi
domestic rabbit monoclonal (C6H3)
  • immunocytochemistry; mouse; 1:1000; fig 5
In order to protect preimplantation mouse embryos from endogenous retrotransposons by histone chaperone CAF-1 mediating repressive histone modifications, Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in immunocytochemistry on mouse samples at 1:1000 (fig 5). Proc Natl Acad Sci U S A (2015) ncbi
domestic rabbit monoclonal (C6H3)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
Cell Signaling Technology G9a antibody (Cell Signaling Technology, 3306) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 1). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (C6H3)
  • chromatin immunoprecipitation; mouse; fig 6
Cell Signaling Technology G9a antibody (cell Signaling Tech, 3306) was used in chromatin immunoprecipitation on mouse samples (fig 6). Nucleic Acids Res (2016) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; human
In order to study the relationship between nuclear LASP-1 and the epigenetic machinery in breast cancer, Cell Signaling Technology G9a antibody (cst, 3306S) was used in western blot on human samples . Oncogene (2016) ncbi
domestic rabbit monoclonal (C6H3)
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:1000. Biochem Biophys Res Commun (2015) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; human; fig 3
Cell Signaling Technology G9a antibody (Cell signaling, 3306) was used in western blot on human samples (fig 3). Ann Surg Oncol (2015) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; rat; 1:500; fig 2
Cell Signaling Technology G9a antibody (Cell signalling technology, 3306) was used in western blot on rat samples at 1:500 (fig 2). J Neurosci (2015) ncbi
domestic rabbit monoclonal (C6H3)
  • immunocytochemistry; human; 1:100; loading ...; fig 3a
In order to use the chromatin in situ proximity technique to identify chromosome 11-specific hubs enriched for H3K9me2, Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in immunocytochemistry on human samples at 1:100 (fig 3a). Epigenetics (2014) ncbi
domestic rabbit monoclonal (C6H3)
  • immunocytochemistry; mouse
Cell Signaling Technology G9a antibody (Cell Signaling Technology, 3306) was used in immunocytochemistry on mouse samples . Blood (2014) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; mouse; 1:1000
Cell Signaling Technology G9a antibody (Cell Signaling Technology, 3306) was used in western blot on mouse samples at 1:1000. Neuroscience (2014) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; mouse; 1:1000
Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in western blot on mouse samples at 1:1000. Mol Endocrinol (2013) ncbi
domestic rabbit monoclonal (C6H3)
  • western blot; mouse; 1:1000
Cell Signaling Technology G9a antibody (Cell Signaling, 3306) was used in western blot on mouse samples at 1:1000. Neurobiol Dis (2013) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:2000; fig 2
MilliporeSigma G9a antibody (Sigma, G6919) was used in western blot on human samples at 1:2000 (fig 2). Nat Commun (2016) ncbi
Articles Reviewed
  1. Pribluda A, Daemen A, Lima A, Wang X, Hafner M, Poon C, et al. EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation. elife. 2022;11: pubmed publisher
  2. Urrutia G, de Assunção T, Mathison A, Salmonson A, Kerketta R, Zeighami A, et al. Inactivation of the Euchromatic Histone-Lysine N-Methyltransferase 2 Pathway in Pancreatic Epithelial Cells Antagonizes Cancer Initiation and Pancreatitis-Associated Promotion by Altering Growth and Immune Gene Expression Networks. Front Cell Dev Biol. 2021;9:681153 pubmed publisher
  3. Pal A, Leung J, Ang G, Rao V, Pignata L, Lim H, et al. EHMT2 epigenetically suppresses Wnt signaling and is a potential target in embryonal rhabdomyosarcoma. elife. 2020;9: pubmed publisher
  4. Dang N, Jiao J, Meng X, An Y, Han C, Huang S. Abnormal overexpression of G9a in melanoma cells promotes cancer progression via upregulation of the Notch1 signaling pathway. Aging (Albany NY). 2020;12:2393-2407 pubmed publisher
  5. Li H, Petersen S, García Mariscal A, Brakebusch C. Negative Regulation of p53-Induced Senescence by N-WASP Is Crucial for DMBA/TPA-Induced Skin Tumor Formation. Cancer Res. 2019;79:2167-2181 pubmed publisher
  6. Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  7. Wang Y, Zhang J, Su Y, Shen Y, Jiang D, Hou Y, et al. G9a regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin. Nat Commun. 2017;8:274 pubmed publisher
  8. Kobayashi T, Zhang H, Tang W, Irie N, Withey S, Klisch D, et al. Principles of early human development and germ cell program from conserved model systems. Nature. 2017;546:416-420 pubmed publisher
  9. Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
  10. Ow J, Palanichamy Kala M, Rao V, Choi M, Bharathy N, Taneja R. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep. 2016;6:34163 pubmed publisher
  11. Olsen J, Wong L, Deimling S, Miles A, Guo H, Li Y, et al. G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports. 2016;7:454-470 pubmed publisher
  12. Rada M, Vasileva E, Lezina L, Marouco D, Antonov A, Macip S, et al. Human EHMT2/G9a activates p53 through methylation-independent mechanism. Oncogene. 2017;36:922-932 pubmed publisher
  13. Dutta A, Le Magnen C, Mitrofanova A, Ouyang X, Califano A, Abate Shen C. Identification of an NKX3.1-G9a-UTY transcriptional regulatory network that controls prostate differentiation. Science. 2016;352:1576-80 pubmed publisher
  14. Rao V, Ow J, Shankar S, Bharathy N, Manikandan J, Wang Y, et al. G9a promotes proliferation and inhibits cell cycle exit during myogenic differentiation. Nucleic Acids Res. 2016;44:8129-43 pubmed publisher
  15. Jayaram H, Hoelper D, Jain S, Cantone N, Lundgren S, Poy F, et al. S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3. Proc Natl Acad Sci U S A. 2016;113:6182-7 pubmed publisher
  16. Zhang X, Peng D, Xi Y, Yuan C, Sagum C, Klein B, et al. G9a-mediated methylation of ERα links the PHF20/MOF histone acetyltransferase complex to hormonal gene expression. Nat Commun. 2016;7:10810 pubmed publisher
  17. Metzger E, Willmann D, McMillan J, Forne I, Metzger P, Gerhardt S, et al. Assembly of methylated KDM1A and CHD1 drives androgen receptor-dependent transcription and translocation. Nat Struct Mol Biol. 2016;23:132-9 pubmed publisher
  18. Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama E, et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A. 2015;112:14641-6 pubmed publisher
  19. Lee J, Lee S, Heo S, Kim K, Kim C, Kim D, et al. Novel Function of Lysine Methyltransferase G9a in the Regulation of Sox2 Protein Stability. PLoS ONE. 2015;10:e0141118 pubmed publisher
  20. Akhade V, Dighe S, Kataruka S, Rao M. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res. 2016;44:387-401 pubmed publisher
  21. Duvall Noelle N, Karwandyar A, Richmond A, Raman D. LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex. Oncogene. 2016;35:1122-33 pubmed publisher
  22. Ren A, Qiu Y, Cui H, Fu G. Inhibition of H3K9 methyltransferase G9a induces autophagy and apoptosis in oral squamous cell carcinoma. Biochem Biophys Res Commun. 2015;459:10-7 pubmed publisher
  23. Hsiao S, Chen M, Chen C, Chien M, Hua K, Hsiao M, et al. The H3K9 Methyltransferase G9a Represses E-cadherin and is Associated with Myometrial Invasion in Endometrial Cancer. Ann Surg Oncol. 2015;22 Suppl 3:S1556-65 pubmed publisher
  24. Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, et al. Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 2015;35:352-65 pubmed publisher
  25. Chen X, Yammine S, Shi C, Tark Dame M, Göndör A, Ohlsson R. The visualization of large organized chromatin domains enriched in the H3K9me2 mark within a single chromosome in a single cell. Epigenetics. 2014;9:1439-45 pubmed publisher
  26. Hattangadi S, Martinez Morilla S, Patterson H, Shi J, Burke K, Avila Figueroa A, et al. Histones to the cytosol: exportin 7 is essential for normal terminal erythroid nuclear maturation. Blood. 2014;124:1931-40 pubmed
  27. Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  28. Jefferson W, Chevalier D, Phelps J, Cantor A, Padilla Banks E, Newbold R, et al. Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure. Mol Endocrinol. 2013;27:1666-77 pubmed publisher
  29. Subbanna S, Shivakumar M, Umapathy N, Saito M, Mohan P, Kumar A, et al. G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain. Neurobiol Dis. 2013;54:475-85 pubmed publisher