This is a Validated Antibody Database (VAD) review about mouse Fmr1, based on 25 published articles (read how Labome selects the articles), using Fmr1 antibody in all methods. It is aimed to help Labome visitors find the most suited Fmr1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Fmr1 synonym: FMRP; Fmr-1

Knockout validation
Abcam
domestic rabbit polyclonal
  • immunocytochemistry knockout validation; mouse; 1:200; loading ...; fig s1a
  • western blot; mouse; loading ...; fig s1b
Abcam Fmr1 antibody (Abcam, ab17722) was used in immunocytochemistry knockout validation on mouse samples at 1:200 (fig s1a) and in western blot on mouse samples (fig s1b). Mol Brain (2019) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; fig s2b
Cell Signaling Technology Fmr1 antibody (Cell Signaling Technology, 4317S) was used in western blot knockout validation on mouse samples (fig s2b). Int J Mol Sci (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D14F4)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
  • immunocytochemistry; mouse; loading ...; fig 1g
Cell Signaling Technology Fmr1 antibody (Cell Signaling, 7104S) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a) and in immunocytochemistry on mouse samples (fig 1g). Mol Neurobiol (2019) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (7G1-1)
  • western blot knockout validation; mouse; loading ...; fig 1a
  • RNA immunoprecipitation; mouse; loading ...; fig 1a
Developmental Studies Hybridoma Bank Fmr1 antibody (DSHB, 7G1-1) was used in western blot knockout validation on mouse samples (fig 1a) and in RNA immunoprecipitation on mouse samples (fig 1a). Mol Neurobiol (2019) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (2F5-1)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 1f
Developmental Studies Hybridoma Bank Fmr1 antibody (DSHB, 2F5-1) was used in immunohistochemistry knockout validation on mouse samples (fig 2a) and in western blot knockout validation on mouse samples (fig 1f). Biol Psychiatry (2017) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (7G1-1)
  • western blot knockout validation; mouse; fig 2c
Developmental Studies Hybridoma Bank Fmr1 antibody (Developmental Studies Hybridoma Bank, 7G1-1) was used in western blot knockout validation on mouse samples (fig 2c). J Neurosci (2011) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:300; loading ...; fig s2d
  • western blot; mouse; 1:1000; loading ...; fig s2a
Abcam Fmr1 antibody (Abcam, ab17722) was used in immunocytochemistry on mouse samples at 1:300 (fig s2d) and in western blot on mouse samples at 1:1000 (fig s2a). Sci Adv (2021) ncbi
domestic rabbit polyclonal
  • RNA immunoprecipitation; mouse; ; loading ...; fig 2a
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig 4h
  • western blot; mouse; 1:1000; loading ...; fig 2d
Abcam Fmr1 antibody (Abcam, ab17722) was used in RNA immunoprecipitation on mouse samples at (fig 2a), in immunohistochemistry - frozen section on mouse samples at 1:400 (fig 4h) and in western blot on mouse samples at 1:1000 (fig 2d). Mol Psychiatry (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:5000; fig 1b
Abcam Fmr1 antibody (Abcam, ab17722) was used in western blot on human samples at 1:5000 (fig 1b). J Biol Chem (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; guinea pig; 1:500; loading ...; fig 2a
Abcam Fmr1 antibody (Abcam, ab17722) was used in immunohistochemistry on guinea pig samples at 1:500 (fig 2a). J Comp Neurol (2020) ncbi
domestic rabbit polyclonal
  • immunocytochemistry knockout validation; mouse; 1:200; loading ...; fig s1a
  • western blot; mouse; loading ...; fig s1b
Abcam Fmr1 antibody (Abcam, ab17722) was used in immunocytochemistry knockout validation on mouse samples at 1:200 (fig s1a) and in western blot on mouse samples (fig s1b). Mol Brain (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1500; loading ...; fig 6d
Abcam Fmr1 antibody (Abcam, ab17722) was used in western blot on human samples at 1:1500 (fig 6d). J Cell Sci (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:200; fig 6a
Abcam Fmr1 antibody (abcam, 17722) was used in immunohistochemistry on human samples at 1:200 (fig 6a). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4b
In order to study the role of GAP and GEF complexing in GTP signaling, Abcam Fmr1 antibody (abcam, ab17722) was used in western blot on mouse samples at 1:1000 (fig 4b). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:1500; loading ...; fig 5a
Abcam Fmr1 antibody (Abcam, ab17722) was used in immunohistochemistry on mouse samples at 1:1500 (fig 5a). RNA Biol (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; rat; 1:500; fig 4
  • RNA immunoprecipitation; mouse; fig 3
In order to investigate how acute ethanol exposure results in lasting antidepressant and anxiolytic behaviors, Abcam Fmr1 antibody (Abcam, ab17722) was used in immunocytochemistry on rat samples at 1:500 (fig 4) and in RNA immunoprecipitation on mouse samples (fig 3). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig s1e
In order to study metabotropic glutamate receptor 5 in a murine model of fragile X syndrome, Abcam Fmr1 antibody (Abcam, ab17722) was used in western blot on human samples at 1:500 (fig s1e). Dev Neurobiol (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:5000
  • western blot; human; 1:1000
Abcam Fmr1 antibody (Abcam, Ab17722) was used in immunohistochemistry - paraffin section on human samples at 1:5000 and in western blot on human samples at 1:1000. J Child Neurol (2016) ncbi
BioLegend
mouse monoclonal (5C2)
  • immunocytochemistry; mouse; fig 2a
BioLegend Fmr1 antibody (BioLegend, 5c2) was used in immunocytochemistry on mouse samples (fig 2a). Mol Psychiatry (2018) ncbi
mouse monoclonal (5C2)
  • immunocytochemistry; human; fig 6c
BioLegend Fmr1 antibody (BioLegend, 834701) was used in immunocytochemistry on human samples (fig 6c). Cell (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (148.1)
  • immunohistochemistry; human; loading ...; fig s5
In order to investigate the role of DNA methylation in fragile X syndrome, Santa Cruz Biotechnology Fmr1 antibody (Santa Cruz, Sc-101048) was used in immunohistochemistry on human samples (fig s5). Stem Cell Reports (2016) ncbi
mouse monoclonal (148.1)
  • western blot; human; 1:200
In order to discuss methods for the characterization of stress granules in virus infected cells, Santa Cruz Biotechnology Fmr1 antibody (Santa Cruz, sc-101048) was used in western blot on human samples at 1:200. Methods (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; fig s2b
Cell Signaling Technology Fmr1 antibody (Cell Signaling Technology, 4317S) was used in western blot knockout validation on mouse samples (fig s2b). Int J Mol Sci (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5f
Cell Signaling Technology Fmr1 antibody (Cell Signaling, 4317) was used in western blot on human samples (fig 5f). elife (2019) ncbi
domestic rabbit monoclonal (D14F4)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
  • immunocytochemistry; mouse; loading ...; fig 1g
Cell Signaling Technology Fmr1 antibody (Cell Signaling, 7104S) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a) and in immunocytochemistry on mouse samples (fig 1g). Mol Neurobiol (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 1c
Cell Signaling Technology Fmr1 antibody (Cell Signaling, 4317) was used in immunocytochemistry on human samples (fig 1c). Cell (2018) ncbi
domestic rabbit monoclonal (D14F4)
  • immunocytochemistry; mouse; loading ...; fig 4b
Cell Signaling Technology Fmr1 antibody (Cell Signaling, 7104) was used in immunocytochemistry on mouse samples (fig 4b). J Virol (2017) ncbi
Phosphosolutions
  • western blot; mouse; 1:500; loading ...; fig 2a
Phosphosolutions Fmr1 antibody (Aurora, p1125-499) was used in western blot on mouse samples at 1:500 (fig 2a). PLoS ONE (2015) ncbi
  • western blot; mouse; 1:1000; fig 1
In order to determine how FMRP S499 is phosphorylated separately from mTORC1-S6K1 activity, Phosphosolutions Fmr1 antibody (PhosphoSolutions, p1125-499) was used in western blot on mouse samples at 1:1000 (fig 1). PLoS ONE (2014) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (2F5-1)
  • immunohistochemistry; mouse; 1:2.5; loading ...; fig 1e
Developmental Studies Hybridoma Bank Fmr1 antibody (DSHB, 2F5-1) was used in immunohistochemistry on mouse samples at 1:2.5 (fig 1e). elife (2020) ncbi
mouse monoclonal (7G1-1)
  • western blot knockout validation; mouse; loading ...; fig 1a
  • RNA immunoprecipitation; mouse; loading ...; fig 1a
Developmental Studies Hybridoma Bank Fmr1 antibody (DSHB, 7G1-1) was used in western blot knockout validation on mouse samples (fig 1a) and in RNA immunoprecipitation on mouse samples (fig 1a). Mol Neurobiol (2019) ncbi
mouse monoclonal (2F5-1)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2a
  • western blot knockout validation; mouse; loading ...; fig 1f
Developmental Studies Hybridoma Bank Fmr1 antibody (DSHB, 2F5-1) was used in immunohistochemistry knockout validation on mouse samples (fig 2a) and in western blot knockout validation on mouse samples (fig 1f). Biol Psychiatry (2017) ncbi
mouse monoclonal (7G1-1)
  • western blot knockout validation; mouse; fig 2c
Developmental Studies Hybridoma Bank Fmr1 antibody (Developmental Studies Hybridoma Bank, 7G1-1) was used in western blot knockout validation on mouse samples (fig 2c). J Neurosci (2011) ncbi
Articles Reviewed
  1. Birsa N, Ule A, Garone M, Tsang B, Mattedi F, Chong P, et al. FUS-ALS mutants alter FMRP phase separation equilibrium and impair protein translation. Sci Adv. 2021;7: pubmed publisher
  2. Heaney C, Namjoshi S, Uneri A, Bach E, Weiner J, Raab Graham K. Role of FMRP in rapid antidepressant effects and synapse regulation. Mol Psychiatry. 2021;26:2350-2362 pubmed publisher
  3. Ha B, Heo J, Jang Y, Park T, Choi J, Jang W, et al. Depletion of Mitochondrial Components from Extracellular Vesicles Secreted from Astrocytes in a Mouse Model of Fragile X Syndrome. Int J Mol Sci. 2021;22: pubmed publisher
  4. Li J, Jiang R, Arendt K, Hsu Y, Zhai S, Chen L. Defective memory engram reactivation underlies impaired fear memory recall in Fragile X syndrome. elife. 2020;9: pubmed publisher
  5. S xe9 vigny M, Bourdeau Julien I, Venkatasubramani J, Hui J, Dutchak P, Sephton C. FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem. 2020;295:18459-18473 pubmed publisher
  6. Shepard K, Korsak L, Debartolo D, Akins M. Axonal localization of the fragile X family of RNA binding proteins is conserved across mammals. J Comp Neurol. 2020;528:502-519 pubmed publisher
  7. Lee F, Lai T, Su P, Liu F. Altered cortical Cytoarchitecture in the Fmr1 knockout mouse. Mol Brain. 2019;12:56 pubmed publisher
  8. Das R, Schwintzer L, Vinopal S, Roca E, Sylvester M, Oprişoreanu A, et al. New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules. J Cell Sci. 2019;: pubmed publisher
  9. Naamati A, Williamson J, Greenwood E, Marelli S, Lehner P, Matheson N. Functional proteomic atlas of HIV infection in primary human CD4+ T cells. elife. 2019;8: pubmed publisher
  10. Yang Y, Arsenault J, Bah A, Krzeminski M, Fekete A, Chao O, et al. Identification of a molecular locus for normalizing dysregulated GABA release from interneurons in the Fragile X brain. Mol Psychiatry. 2018;: pubmed publisher
  11. Chmielewska J, Kuzniewska B, Milek J, Urbanska K, Dziembowska M. Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage. Mol Neurobiol. 2019;56:2741-2759 pubmed publisher
  12. Liu X, Wu H, Krzisch M, Wu X, Graef J, Muffat J, et al. Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Cell. 2018;172:979-992.e6 pubmed publisher
  13. Green K, Glineburg M, Kearse M, Flores B, Linsalata A, Fedak S, et al. RAN translation at C9orf72-associated repeat expansions is selectively enhanced by the integrated stress response. Nat Commun. 2017;8:2005 pubmed publisher
  14. Wilkinson B, Li J, Coba M. Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling. Sci Rep. 2017;7:5272 pubmed publisher
  15. Filippini A, Bonini D, Lacoux C, Pacini L, Zingariello M, Sancillo L, et al. Absence of the Fragile X Mental Retardation Protein results in defects of RNA editing of neuronal mRNAs in mouse. RNA Biol. 2017;14:1580-1591 pubmed publisher
  16. Frolov I, Kim D, Akhrymuk M, Mobley J, Frolova E. Hypervariable Domain of Eastern Equine Encephalitis Virus nsP3 Redundantly Utilizes Multiple Cellular Proteins for Replication Complex Assembly. J Virol. 2017;91: pubmed publisher
  17. Hodges J, Yu X, Gilmore A, Bennett H, Tjia M, Perna J, et al. Astrocytic Contributions to Synaptic and Learning Abnormalities in a Mouse Model of Fragile X Syndrome. Biol Psychiatry. 2017;82:139-149 pubmed publisher
  18. Brykczynska U, Pecho Vrieseling E, Thiemeyer A, Klein J, Fruh I, Doll T, et al. CGG Repeat-Induced FMR1 Silencing Depends on the Expansion Size in Human iPSCs and Neurons Carrying Unmethylated Full Mutations. Stem Cell Reports. 2016;7:1059-1071 pubmed publisher
  19. Wolfe S, Workman E, Heaney C, Niere F, Namjoshi S, Cacheaux L, et al. FMRP regulates an ethanol-dependent shift in GABABR function and expression with rapid antidepressant properties. Nat Commun. 2016;7:12867 pubmed publisher
  20. Achuta V, Grym H, Putkonen N, Louhivuori V, Kärkkäinen V, Koistinaho J, et al. Metabotropic glutamate receptor 5 responses dictate differentiation of neural progenitors to NMDA-responsive cells in fragile X syndrome. Dev Neurobiol. 2017;77:438-453 pubmed publisher
  21. Reynolds C, Smith G, Jefferson T, Lugo J. Comparison of Equivalence between Two Commercially Available S499-Phosphorylated FMRP Antibodies in Mice. PLoS ONE. 2015;10:e0143134 pubmed publisher
  22. Lechpammer M, Wintermark P, Merry K, Jackson M, Jantzie L, Jensen F. Dysregulation of FMRP/mTOR Signaling Cascade in Hypoxic-Ischemic Injury of Premature Human Brain. J Child Neurol. 2016;31:426-32 pubmed publisher
  23. Panas M, Kedersha N, McInerney G. Methods for the characterization of stress granules in virus infected cells. Methods. 2015;90:57-64 pubmed publisher
  24. Bartley C, O Keefe R, Bordey A. FMRP S499 is phosphorylated independent of mTORC1-S6K1 activity. PLoS ONE. 2014;9:e96956 pubmed publisher
  25. Xu X, Zong R, Li Z, Biswas M, Fang Z, Nelson D, et al. FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124. J Neurosci. 2011;31:13705-9 pubmed publisher