This is a Validated Antibody Database (VAD) review about mouse Flt4, based on 25 published articles (read how Labome selects the articles), using Flt4 antibody in all methods. It is aimed to help Labome visitors find the most suited Flt4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Flt4 synonym: AI323512; Chy; Flt-4; VEGFR-3; VEGFR3; vascular endothelial growth factor receptor 3; chylous ascites; receptor protein tyrosine kinase; tyrosine-protein kinase receptor FLT4

R&D Systems
goat polyclonal
  • immunohistochemistry - frozen section; mouse; fig s1a
  • immunohistochemistry; mouse
R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples (fig s1a) and in immunohistochemistry on mouse samples . Proc Natl Acad Sci U S A (2018) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3c
R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples (fig s3c). J Clin Invest (2017) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s1c
R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - paraffin section on mouse samples (fig s1c). Nature (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; loading ...; fig 2j
In order to examine the role of metabolism in lymphatic development, R&D Systems Flt4 antibody (R&D, AF743) was used in immunohistochemistry on mouse samples (fig 2j). Nature (2017) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig s5b
  • western blot; mouse; loading ...; fig 3i
R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples (fig s5b) and in western blot on mouse samples (fig 3i). J Clin Invest (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5b
In order to report that SDC4 governs flow-induced lymphatic endothelial cell polarization via regulation of VANGL2 expression, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry on mouse samples at 1:100 (fig 5b). Development (2016) ncbi
goat polyclonal
  • flow cytometry; mouse; fig 1
R&D Systems Flt4 antibody (R&D systems, AF743) was used in flow cytometry on mouse samples (fig 1). Arthritis Res Ther (2016) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; fig 1
In order to compare valve phenotypes of Cx37, Cx43, and Cx47 knockout mice by NFATc1 and connexin expression in normal developing venous valves, segregated Foxc2, and connexin-specific differences, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples (fig 1). Dev Biol (2016) ncbi
goat polyclonal
  • blocking or activating experiments; mouse; 1:200; fig 2
  • immunocytochemistry; mouse; 1:200; fig 6
R&D Systems Flt4 antibody (AngioBio, AF743) was used in blocking or activating experiments on mouse samples at 1:200 (fig 2) and in immunocytochemistry on mouse samples at 1:200 (fig 6). Nat Commun (2016) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; fig 2
In order to analyze mediation of pathological retinal neovascularization via modulating DLL4-NOTCH1 signaling due to the cyclic AMP response element binding protein, R&D Systems Flt4 antibody (R & D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples (fig 2). EBioMedicine (2015) ncbi
goat polyclonal
  • immunohistochemistry; mouse; fig 5
In order to determine the roles of Sox7, Sox17, and Sox18 in the developing and mature mouse vasculature, R&D Systems Flt4 antibody (R and D Systems, AF743) was used in immunohistochemistry on mouse samples (fig 5). PLoS ONE (2015) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 3
R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 3). Angiogenesis (2016) ncbi
goat polyclonal
  • western blot; human
In order to assess the role of Galphaq/11 in VEGF-dependent regulation of vascular permeability and angiogenesis, R&D Systems Flt4 antibody (Francisco, AF743) was used in western blot on human samples . Cardiovasc Res (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1h
In order to find that cystogenesis is associated with a disorganized pericystic network of vessels expressing platelet/endothelial cell adhesion molecule 1 and vascular endothelial growth factor receptor 3, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples (fig 1h). J Am Soc Nephrol (2016) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2
In order to investigate the mechanisms that regulate the entrance and exit of immune cells from the central nervous system, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2). Nature (2015) ncbi
goat polyclonal
  • western blot; mouse; fig s10
In order to demonstrate that the Cdk5-Foxc2 interaction regulates lymphatic vessel development, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in western blot on mouse samples (fig s10). Nat Commun (2015) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:50; fig 1
R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry on mouse samples at 1:50 (fig 1). Nature (2015) ncbi
goat polyclonal
  • western blot; human
In order to study the role of VE-cadherin in fluid shear stress, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in western blot on human samples . J Cell Biol (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse
In order to investigate the role of Cx37 during vasculogenesis in mice, R&D Systems Flt4 antibody (R&D Systems, AF743) was used in immunohistochemistry - frozen section on mouse samples . Dev Biol (2013) ncbi
Invitrogen
rat monoclonal (AFL4)
  • immunohistochemistry; mouse; loading ...; fig 2j
In order to examine the role of metabolism in lymphatic development, Invitrogen Flt4 antibody (eBioscience, 14-5988-82) was used in immunohistochemistry on mouse samples (fig 2j). Nature (2017) ncbi
rat monoclonal (AFL4)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 4b
In order to observe that Vegf-d promotes oedema in response to hyperoxia in mice, Invitrogen Flt4 antibody (eBioscience, 14-5988-81) was used in immunohistochemistry - paraffin section on mouse samples (fig 4b). J Pathol (2016) ncbi
rat monoclonal (AFL4)
  • immunohistochemistry; mouse
Invitrogen Flt4 antibody (eBioscience, 14-5988-81) was used in immunohistochemistry on mouse samples . PLoS ONE (2013) ncbi
rat monoclonal (AFL4)
  • immunohistochemistry; mouse; fig 4
In order to investigate the function of Prox1 in vascular endothelial cells in vivo, Invitrogen Flt4 antibody (eBiosciences, 16-5988) was used in immunohistochemistry on mouse samples (fig 4). BMC Dev Biol (2010) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 6a
Abcam Flt4 antibody (Abcam, ab27278) was used in immunohistochemistry - frozen section on mouse samples (fig 6a). BMC Cancer (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200
In order to investigate the effect of administering chloropyramine hydrochloride, which disrupts the FAK-VEGFR-3 interaction, on neuroblastoma, Abcam Flt4 antibody (Abcam, ab27278) was used in immunohistochemistry - paraffin section on human samples at 1:200. Mol Carcinog (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-3)
  • western blot; mouse; 1:200; fig 2
Santa Cruz Biotechnology Flt4 antibody (Santa Cruz, sc-365748) was used in western blot on mouse samples at 1:200 (fig 2). Arch Med Sci (2016) ncbi
Articles Reviewed
  1. Souma T, Thomson B, Heinen S, Carota I, Yamaguchi S, Onay T, et al. Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP. Proc Natl Acad Sci U S A. 2018;115:1298-1303 pubmed publisher
  2. Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
  3. Olmeda D, Cerezo Wallis D, Riveiro Falkenbach E, Pennacchi P, Contreras Alcalde M, Ibarz N, et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature. 2017;546:676-680 pubmed publisher
  4. Wong B, Wang X, Zecchin A, Thienpont B, Cornelissen I, Kalucka J, et al. The role of fatty acid ?-oxidation in lymphangiogenesis. Nature. 2017;542:49-54 pubmed publisher
  5. Lee S, Rho S, Park H, Park J, Kim J, Lee I, et al. Carbohydrate-binding protein CLEC14A regulates VEGFR-2- and VEGFR-3-dependent signals during angiogenesis and lymphangiogenesis. J Clin Invest. 2017;127:457-471 pubmed publisher
  6. Wang Y, Baeyens N, Corti F, Tanaka K, Fang J, Zhang J, et al. Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development. Development. 2016;143:4441-4451 pubmed
  7. Yue T, Zheng X, Dou Y, Zheng X, Sun R, Tian Z, et al. Interleukin 12 shows a better curative effect on lung cancer than paclitaxel and cisplatin doublet chemotherapy. BMC Cancer. 2016;16:665 pubmed publisher
  8. Zhu Y, Wu Y, Liang Y, Tan W, Liu Z, Xiao J. Regulation of expression level of fms-like tyrosine kinase-4 is related to osteoclast differentiation. Arch Med Sci. 2016;12:502-6 pubmed publisher
  9. Liang Q, Ju Y, Chen Y, Wang W, Li J, Zhang L, et al. Lymphatic endothelial cells efferent to inflamed joints produce iNOS and inhibit lymphatic vessel contraction and drainage in TNF-induced arthritis in mice. Arthritis Res Ther. 2016;18:62 pubmed publisher
  10. Munger S, Geng X, Srinivasan R, Witte M, Paul D, Simon A. Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice. Dev Biol. 2016;412:173-90 pubmed publisher
  11. Wong H, Jin G, Cao R, Zhang S, Cao Y, Zhou Z. MT1-MMP sheds LYVE-1 on lymphatic endothelial cells and suppresses VEGF-C production to inhibit lymphangiogenesis. Nat Commun. 2016;7:10824 pubmed publisher
  12. Sato T, Paquet Fifield S, Harris N, Roufail S, Turner D, Yuan Y, et al. VEGF-D promotes pulmonary oedema in hyperoxic acute lung injury. J Pathol. 2016;239:152-61 pubmed publisher
  13. Singh N, Kotla S, Kumar R, Rao G. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine. 2015;2:1767-84 pubmed publisher
  14. Zhou Y, Williams J, Smallwood P, Nathans J. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE. 2015;10:e0143650 pubmed publisher
  15. Janssen L, Dupont L, Bekhouche M, Noel A, Leduc C, Voz M, et al. ADAMTS3 activity is mandatory for embryonic lymphangiogenesis and regulates placental angiogenesis. Angiogenesis. 2016;19:53-65 pubmed publisher
  16. Sivaraj K, Li R, Albarrán Juárez J, Wang S, Tischner D, Grimm M, et al. Endothelial Gαq/11 is required for VEGF-induced vascular permeability and angiogenesis. Cardiovasc Res. 2015;108:171-80 pubmed publisher
  17. Huang J, Woolf A, Kolatsi Joannou M, Baluk P, Sandford R, Peters D, et al. Vascular Endothelial Growth Factor C for Polycystic Kidney Diseases. J Am Soc Nephrol. 2016;27:69-77 pubmed publisher
  18. Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, et al. Structural and functional features of central nervous system lymphatic vessels. Nature. 2015;523:337-41 pubmed publisher
  19. Liebl J, Zhang S, Moser M, Agalarov Y, Demir C, Hager B, et al. Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2. Nat Commun. 2015;6:7274 pubmed publisher
  20. Klotz L, Norman S, Vieira J, Masters M, Rohling M, Dubé K, et al. Cardiac lymphatics are heterogeneous in origin and respond to injury. Nature. 2015;522:62-7 pubmed
  21. Coon B, Baeyens N, Han J, Budatha M, Ross T, Fang J, et al. Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex. J Cell Biol. 2015;208:975-86 pubmed publisher
  22. Dellinger M, Meadows S, Wynne K, Cleaver O, Brekken R. Vascular endothelial growth factor receptor-2 promotes the development of the lymphatic vasculature. PLoS ONE. 2013;8:e74686 pubmed publisher
  23. Stewart J, Ma X, Megison M, Nabers H, Cance W, Kurenova E, et al. Inhibition of FAK and VEGFR-3 binding decreases tumorigenicity in neuroblastoma. Mol Carcinog. 2015;54:9-23 pubmed publisher
  24. Munger S, Kanady J, Simon A. Absence of venous valves in mice lacking Connexin37. Dev Biol. 2013;373:338-48 pubmed publisher
  25. Kim H, Nguyen V, Petrova T, Cruz M, Alitalo K, Dumont D. Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature. BMC Dev Biol. 2010;10:72 pubmed publisher