This is a Validated Antibody Database (VAD) review about mouse Flt1, based on 21 published articles (read how Labome selects the articles), using Flt1 antibody in all methods. It is aimed to help Labome visitors find the most suited Flt1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Flt1 synonym: AI323757; Flt-1; VEGFR-1; VEGFR1; sFlt1

Abcam
domestic rabbit monoclonal
  • western blot; rat; 1:2000; loading ...; fig 2h, 3g
Abcam Flt1 antibody (Abcam, ab184784) was used in western blot on rat samples at 1:2000 (fig 2h, 3g). Exp Ther Med (2021) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; domestic rabbit; 1:5000; loading ...; fig 8b
Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on domestic rabbit samples at 1:5000 (fig 8b). Invest Ophthalmol Vis Sci (2020) ncbi
domestic rabbit monoclonal (Y103)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4c
  • western blot; mouse; 1:1000; loading ...; fig 6
Abcam Flt1 antibody (Abcam, ab32152) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4c) and in western blot on mouse samples at 1:1000 (fig 6). Polymers (Basel) (2020) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human; loading ...; fig 5d
Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on human samples (fig 5d). Br J Cancer (2019) ncbi
domestic rabbit monoclonal (Y103)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig e10j
Abcam Flt1 antibody (Abcam, ab32152) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig e10j). Nature (2019) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human; fig s5c
Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on human samples (fig s5c). Cell Rep (2019) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; rat; 1:10,000; loading ...; fig 1b
In order to suggest that functional neovasculature requires NO-Tie2 and VEGF signaling, Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on rat samples at 1:10,000 (fig 1b). J Physiol (2017) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; mouse; fig 6
Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on mouse samples (fig 6). Sci Rep (2016) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human; fig 6
  • western blot; rat; fig 1
Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on human samples (fig 6) and in western blot on rat samples (fig 1). Sci Rep (2016) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; mouse
In order to assess the role of receptor-interacting protein 2 in VEGF signalling and myocardial ischaemia/reperfusion injury, Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on mouse samples . Cardiovasc Res (2015) ncbi
domestic rabbit monoclonal (Y103)
  • immunocytochemistry; human
  • immunohistochemistry; human
Abcam Flt1 antibody (Abcam, ab32152) was used in immunocytochemistry on human samples and in immunohistochemistry on human samples . Biomaterials (2014) ncbi
domestic rabbit monoclonal (Y103)
  • flow cytometry; rat
  • immunocytochemistry; rat
Abcam Flt1 antibody (Abcam, ab32152) was used in flow cytometry on rat samples and in immunocytochemistry on rat samples . Vasc Cell (2014) ncbi
domestic rabbit monoclonal (Y103)
  • western blot; human
Abcam Flt1 antibody (Abcam, ab32152) was used in western blot on human samples . Mol Cancer Ther (2013) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 6a
R&D Systems Flt1 antibody (R&D, AF471) was used in immunohistochemistry on mouse samples at 1:200 (fig 6a). Biomolecules (2021) ncbi
rat monoclonal (141522)
  • flow cytometry; human; fig s5a
In order to demonstrate that IGF2 secreted by inhibitor of differentiation-overexpressing oesophageal cancer cells instigates VEGFR1-positive bone marrow cells in the tumor macroenvironment, R&D Systems Flt1 antibody (R&D Systems, FAB4711P) was used in flow cytometry on human samples (fig s5a). Nat Commun (2017) ncbi
rat monoclonal (141522)
  • flow cytometry; mouse; loading ...; fig 2b
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, R&D Systems Flt1 antibody (R&D, 141522) was used in flow cytometry on mouse samples (fig 2b). Circ Res (2017) ncbi
rat monoclonal (141522)
  • flow cytometry; mouse; loading ...; fig st2
In order to demonstrate that plasmalemma vesicle-associated protein governs the seeding of fetal monocyte-derived macrophages in the tissues of mice, R&D Systems Flt1 antibody (R&D Systems, FAB4711P) was used in flow cytometry on mouse samples (fig st2). Nature (2016) ncbi
rat monoclonal (141522)
  • flow cytometry; mouse; 1:50; loading ...; fig 2g
In order to demonstrate that endothelial production of PDGF-CC during white adipose tissue angiogenesis regulates browning, R&D Systems Flt1 antibody (R&D, FAB4711G) was used in flow cytometry on mouse samples at 1:50 (fig 2g). Nat Commun (2016) ncbi
domestic goat polyclonal
In order to analyze ischemia-induced brain damage due to semaphorin3A increasing vascular permeability, R&D Systems Flt1 antibody (R&D Systems, AF471) was used . Sci Rep (2015) ncbi
rat monoclonal (141522)
  • flow cytometry; mouse; fig 2
R&D Systems Flt1 antibody (R&D Systems, 141522) was used in flow cytometry on mouse samples (fig 2). J Exp Med (2015) ncbi
rat monoclonal (141522)
  • flow cytometry; mouse
R&D Systems Flt1 antibody (R&D, 141522) was used in flow cytometry on mouse samples . Sci Rep (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 5e
  • western blot; human; loading ...; fig 5e
  • immunoprecipitation; mouse; loading ...; fig 3b
  • western blot; mouse; loading ...; fig 3b
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, Invitrogen Flt1 antibody (ThermoFisher, PA5-16493) was used in immunoprecipitation on human samples (fig 5e), in western blot on human samples (fig 5e), in immunoprecipitation on mouse samples (fig 3b) and in western blot on mouse samples (fig 3b). Circ Res (2017) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse
  • flow cytometry; human; loading ...; fig 4a
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, MilliporeSigma Flt1 antibody (Sigma, SAB4504006) was used in immunohistochemistry - paraffin section on mouse samples , in western blot on mouse samples and in flow cytometry on human samples (fig 4a). Circ Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig s7
In order to find that that vascular endothelial growth factor 165b prevents activation of vascular endothelial growth factor receptor 1-STAT3 signaling by vascular endothelial growth factor 165a, MilliporeSigma Flt1 antibody (Sigma, SAB4504649) was used in western blot on mouse samples (fig s7). Circ Res (2017) ncbi
Articles Reviewed
  1. Zhan C, Sun Y, Pan J, Chen L, Yuan T. Effect of the Notch4/Dll4 signaling pathway in early gestational intrauterine infection on lung development. Exp Ther Med. 2021;22:972 pubmed publisher
  2. Tirronen A, Downes N, Huusko J, Laakkonen J, Tuomainen T, Tavi P, et al. The Ablation of VEGFR-1 Signaling Promotes Pressure Overload-Induced Cardiac Dysfunction and Sudden Death. Biomolecules. 2021;11: pubmed publisher
  3. Wang Y, Gao G, Wu Y, Wang Y, Wu X, Zhou Q. S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway. Invest Ophthalmol Vis Sci. 2020;61:19 pubmed publisher
  4. Zanchetta F, Trinca R, Gomes Silva J, Breder J, Cantarutti T, Consonni S, et al. Effects of Electrospun Fibrous Membranes of PolyCaprolactone and Chitosan/Poly(Ethylene Oxide) on Mouse Acute Skin Lesions. Polymers (Basel). 2020;12: pubmed publisher
  5. Chen Q, Yang C, Chen L, Zhang J, Ge W, Yuan H, et al. YY1 targets tubulin polymerisation-promoting protein to inhibit migration, invasion and angiogenesis in pancreatic cancer via p38/MAPK and PI3K/AKT pathways. Br J Cancer. 2019;121:912-921 pubmed publisher
  6. Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  7. Njah K, Chakraborty S, Qiu B, Arumugam S, Raju A, Pobbati A, et al. A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis. Cell Rep. 2019;28:949-965.e7 pubmed publisher
  8. Xu W, Li B, Guan X, Chung S, Wang Y, Yip Y, et al. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression. Nat Commun. 2017;8:14399 pubmed publisher
  9. Ganta V, Choi M, Kutateladze A, Annex B. VEGF165b Modulates Endothelial VEGFR1-STAT3 Signaling Pathway and Angiogenesis in Human and Experimental Peripheral Arterial Disease. Circ Res. 2017;120:282-295 pubmed publisher
  10. Stone O, Carter J, Lin P, Paleolog E, Machado M, Bates D. Differential regulation of blood flow-induced neovascularization and mural cell recruitment by vascular endothelial growth factor and angiopoietin signalling. J Physiol. 2017;595:1575-1591 pubmed publisher
  11. Rantakari P, Jäppinen N, Lokka E, Mokkala E, Gerke H, Peuhu E, et al. Fetal liver endothelium regulates the seeding of tissue-resident macrophages. Nature. 2016;538:392-396 pubmed publisher
  12. Seki T, Hosaka K, Lim S, Fischer C, Honek J, Yang Y, et al. Endothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat. Nat Commun. 2016;7:12152 pubmed publisher
  13. Chung S, Gillies M, Yam M, Wang Y, Shen W. Differential expression of microRNAs in retinal vasculopathy caused by selective Müller cell disruption. Sci Rep. 2016;6:28993 pubmed publisher
  14. Huang D, Zhao C, Ju R, Kumar A, Tian G, Huang L, et al. VEGF-B inhibits hyperglycemia- and Macugen-induced retinal apoptosis. Sci Rep. 2016;6:26059 pubmed publisher
  15. Andersson L, Scharin Täng M, Lundqvist A, Lindbom M, Mardani I, Fogelstrand P, et al. Rip2 modifies VEGF-induced signalling and vascular permeability in myocardial ischaemia. Cardiovasc Res. 2015;107:478-86 pubmed publisher
  16. Hou S, Nilchi L, Li X, Gangaraju S, Jiang S, Aylsworth A, et al. Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage. Sci Rep. 2015;5:7890 pubmed publisher
  17. Voron T, Colussi O, Marcheteau E, Pernot S, Nizard M, Pointet A, et al. VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors. J Exp Med. 2015;212:139-48 pubmed publisher
  18. Weston W, Zayas J, Perez R, George J, Jurecic R. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep. 2014;4:5199 pubmed publisher
  19. Dirisala A, Osada K, Chen Q, Tockary T, Machitani K, Osawa S, et al. Optimized rod length of polyplex micelles for maximizing transfection efficiency and their performance in systemic gene therapy against stroma-rich pancreatic tumors. Biomaterials. 2014;35:5359-5368 pubmed publisher
  20. Kennedy E, Hakimjavadi R, Greene C, Mooney C, Fitzpatrick E, Collins L, et al. Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?. Vasc Cell. 2014;6:6 pubmed publisher
  21. Sreevalsan S, Safe S. The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells. Mol Cancer Ther. 2013;12:2483-93 pubmed publisher