This is a Validated Antibody Database (VAD) review about mouse Fgf21, based on 19 published articles (read how Labome selects the articles), using Fgf21 antibody in all methods. It is aimed to help Labome visitors find the most suited Fgf21 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
domestic rabbit monoclonal (EPR8314(2))
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1d
Abcam Fgf21 antibody (Abcam, ab171941) was used in immunohistochemistry knockout validation on mouse samples (fig 1d). Cell Rep (2022) ncbi
Abcam
domestic rabbit monoclonal (EPR8314(2))
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1d
Abcam Fgf21 antibody (Abcam, ab171941) was used in immunohistochemistry knockout validation on mouse samples (fig 1d). Cell Rep (2022) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:1000; fig 1g
Abcam Fgf21 antibody (AbCam, ab171941) was used in western blot on mouse samples at 1:1000 (fig 1g). Oxid Med Cell Longev (2021) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; loading ...; fig 1b
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples (fig 1b). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 7c
Abcam Fgf21 antibody (Abcam, ab171941) was used in immunohistochemistry - frozen section on mouse samples at 1:250 (fig 7c). Sci Rep (2021) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:1000; loading ...
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples at 1:1000. elife (2021) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:4000; loading ...; fig 7f
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples at 1:4000 (fig 7f). Br J Pharmacol (2021) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; human; 1:1000; loading ...; fig 2e
  • immunohistochemistry - paraffin section; mouse; 1:250; loading ...; fig 3e
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on human samples at 1:1000 (fig 2e) and in immunohistochemistry - paraffin section on mouse samples at 1:250 (fig 3e). EBioMedicine (2019) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; loading ...; fig 3b
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples (fig 3b). Life Sci (2018) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; human; 1:2000; loading ...; fig 3b
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on human samples at 1:2000 (fig 3b). PLoS ONE (2018) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; human; 1:1000; loading ...; fig 5a
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on human samples at 1:1000 (fig 5a). Stem Cells Int (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3c
Abcam Fgf21 antibody (Abcam, ab64857) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 3c). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:1000; fig 7a
In order to study the function of OPA1 in FGF21 secretion from muscle, Abcam Fgf21 antibody (Abcam, Ab171941) was used in western blot on mouse samples at 1:1000 (fig 7a). EMBO J (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 2c
  • ELISA; mouse; loading ...; fig 3a
Abcam Fgf21 antibody (Abcam, Ab64857) was used in immunohistochemistry on mouse samples at 1:1000 (fig 2c) and in ELISA on mouse samples (fig 3a). Cell Rep (2017) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:1000; loading ...; fig 1f
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples at 1:1000 (fig 1f). Endocrinology (2017) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; loading ...; fig 1a
Abcam Fgf21 antibody (Cell signaling, ab171941) was used in western blot on mouse samples (fig 1a). PLoS ONE (2016) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; loading ...; fig s5b
Abcam Fgf21 antibody (abcam, ab171941) was used in western blot on mouse samples (fig s5b). Cell Rep (2016) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:500
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples at 1:500. Science (2015) ncbi
domestic rabbit monoclonal (EPR8314(2))
  • western blot; mouse; 1:1000; fig 3
Abcam Fgf21 antibody (Abcam, ab171941) was used in western blot on mouse samples at 1:1000 (fig 3). Cell Metab (2015) ncbi
Invitrogen
domestic rabbit recombinant (JA10-97)
  • western blot; human; loading ...; fig 2a
Invitrogen Fgf21 antibody (Life Technologies, MA5-32652) was used in western blot on human samples (fig 2a). Adv Sci (Weinh) (2021) ncbi
Articles Reviewed
  1. Zhou B, Claflin K, Flippo K, Sullivan A, Asghari A, Tadinada S, et al. Central FGF21 production regulates memory but not peripheral metabolism. Cell Rep. 2022;40:111239 pubmed publisher
  2. Pan J, Lin H, Yu J, Zhang H, Zhang J, Wang C, et al. MiR-21-3p Inhibits Adipose Browning by Targeting FGFR1 and Aggravates Atrial Fibrosis in Diabetes. Oxid Med Cell Longev. 2021;2021:9987219 pubmed publisher
  3. Christidis G, Karatayli E, Hall R, Weber S, Reichert M, Hohl M, et al. Fibroblast Growth Factor 21 Response in a Preclinical Alcohol Model of Acute-on-Chronic Liver Injury. Int J Mol Sci. 2021;22: pubmed publisher
  4. Bayoumi A, Elsayed A, Han S, Petta S, Adams L, Aller R, et al. Mistranslation Drives Alterations in Protein Levels and the Effects of a Synonymous Variant at the Fibroblast Growth Factor 21 Locus. Adv Sci (Weinh). 2021;8:2004168 pubmed publisher
  5. Homer Bouthiette C, Xiao L, Hurley M. Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice. Sci Rep. 2021;11:11005 pubmed publisher
  6. Pereira R, Marti A, Olvera A, Tadinada S, Bjorkman S, Weatherford E, et al. OPA1 deletion in brown adipose tissue improves thermoregulation and systemic metabolism via FGF21. elife. 2021;10: pubmed publisher
  7. Hua X, Sun D, Zhang W, Fu J, Tong J, Sun S, et al. P7C3-A20 alleviates fatty liver by shaping gut microbiota and inducing FGF21/FGF1, via the AMP-activated protein kinase/CREB regulated transcription coactivator 2 pathway. Br J Pharmacol. 2021;178:2111-2130 pubmed publisher
  8. Liu J, Yang K, Yang J, Xiao W, Le Y, Yu F, et al. Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output. EBioMedicine. 2019;: pubmed publisher
  9. Zhang X, Zhuang R, Wu H, Chen J, Wang F, Li G, et al. A novel role of endocan in alleviating LPS-induced acute lung injury. Life Sci. 2018;202:89-97 pubmed publisher
  10. Vargas D, Lopez C, Acero E, Benítez E, Wintaco A, Camacho J, et al. Thermogenic capacity of human periaortic adipose tissue is transformed by body weight. PLoS ONE. 2018;13:e0194269 pubmed publisher
  11. Lizcano F, Vargas D, Gomez A, Torrado A. Human ADMC-Derived Adipocyte Thermogenic Capacity Is Regulated by IL-4 Receptor. Stem Cells Int. 2017;2017:2767916 pubmed publisher
  12. Kuroda M, Muramatsu R, Maedera N, Koyama Y, Hamaguchi M, Fujimura H, et al. Peripherally derived FGF21 promotes remyelination in the central nervous system. J Clin Invest. 2017;127:3496-3509 pubmed publisher
  13. Pereira R, Tadinada S, Zasadny F, Oliveira K, Pires K, Olvera A, et al. OPA1 deficiency promotes secretion of FGF21 from muscle that prevents obesity and insulin resistance. EMBO J. 2017;36:2126-2145 pubmed publisher
  14. Ng R, Hussain N, Zhang Q, Chang C, Li H, Fu Y, et al. miRNA-32 Drives Brown Fat Thermogenesis and Trans-activates Subcutaneous White Fat Browning in Mice. Cell Rep. 2017;19:1229-1246 pubmed publisher
  15. Zeng K, Tian L, Patel R, Shao W, Song Z, Liu L, et al. Diet Polyphenol Curcumin Stimulates Hepatic Fgf21 Production and Restores Its Sensitivity in High-Fat-Diet-Fed Male Mice. Endocrinology. 2017;158:277-292 pubmed publisher
  16. Ziros P, Zagoriti Z, Lagoumintzis G, Kyriazopoulou V, Iskrenova R, Habeos E, et al. Hepatic Fgf21 Expression Is Repressed after Simvastatin Treatment in Mice. PLoS ONE. 2016;11:e0162024 pubmed publisher
  17. Tsai S, Rodriguez A, Dastidar S, Del Greco E, Carr K, Sitzmann J, et al. Increased 4E-BP1 Expression Protects against Diet-Induced Obesity and Insulin Resistance in Male Mice. Cell Rep. 2016;16:1903-14 pubmed publisher
  18. Gong G, Song M, Csordás G, Kelly D, Matkovich S, Dorn G. Parkin-mediated mitophagy directs perinatal cardiac metabolic maturation in mice. Science. 2015;350:aad2459 pubmed publisher
  19. Song M, Mihara K, Chen Y, Scorrano L, Dorn G. Mitochondrial fission and fusion factors reciprocally orchestrate mitophagic culling in mouse hearts and cultured fibroblasts. Cell Metab. 2015;21:273-85 pubmed publisher