This is a Validated Antibody Database (VAD) review about mouse Ephb2, based on 19 published articles (read how Labome selects the articles), using Ephb2 antibody in all methods. It is aimed to help Labome visitors find the most suited Ephb2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ephb2 synonym: Cek5; Drt; ETECK; Erk; Hek5; Nuk; Prkm5; Qek5; Sek3; Tyro5; ephrin type-B receptor 2; neural kinase; nuk receptor tyrosine kinase; tyrosine-protein kinase receptor EPH-3; tyrosine-protein kinase receptor SEK-3

Knockout validation
R&D Systems
goat polyclonal
  • western blot knockout validation; mouse; 1:500; fig 2c
In order to determine how ephrin-B3 coordinates amygdala spinogenesis and timed axon targeting for innate fear behavior, R&D Systems Ephb2 antibody (R&D, P54763) was used in western blot knockout validation on mouse samples at 1:500 (fig 2c). Nat Commun (2016) ncbi
R&D Systems
rat monoclonal (512012)
  • flow cytometry; mouse; fig 1b
R&D Systems Ephb2 antibody (R&D Systems, 512012) was used in flow cytometry on mouse samples (fig 1b). Clin Cancer Res (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1f
In order to show that ephrin-B2 antagonizes the pathogenic effects of patients' N-methyl-D-aspartate receptor antibodies on memory and synaptic plasticity, R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in immunohistochemistry on mouse samples at 1:100 (fig 1f). Ann Neurol (2016) ncbi
goat polyclonal
  • immunoprecipitation; mouse; fig 1f
  • western blot; mouse; fig 1f
In order to investigate the impact of exosomes on ephrin-Eph signaling, R&D Systems Ephb2 antibody (R&D, AF467) was used in immunoprecipitation on mouse samples (fig 1f) and in western blot on mouse samples (fig 1f). J Cell Biol (2016) ncbi
goat polyclonal
  • western blot; human; 1:100; fig 1
  • western blot; mouse; 1:100; fig 1
In order to study the attenuation of Alzheimer's pathology in mice due to kallikrein-8 inhibition, R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in western blot on human samples at 1:100 (fig 1) and in western blot on mouse samples at 1:100 (fig 1). Alzheimers Dement (2016) ncbi
goat polyclonal
  • western blot knockout validation; mouse; 1:500; fig 2c
In order to determine how ephrin-B3 coordinates amygdala spinogenesis and timed axon targeting for innate fear behavior, R&D Systems Ephb2 antibody (R&D, P54763) was used in western blot knockout validation on mouse samples at 1:500 (fig 2c). Nat Commun (2016) ncbi
goat polyclonal
  • immunocytochemistry; rat; 1:100; fig 2
R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in immunocytochemistry on rat samples at 1:100 (fig 2). Neuron (2015) ncbi
goat polyclonal
  • western blot; mouse; loading ...; fig 2g
R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in western blot on mouse samples (fig 2g). J Biol Chem (2016) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; human; 1:50; fig 2b
R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 2b). PLoS ONE (2015) ncbi
goat polyclonal
  • immunohistochemistry - paraffin section; mouse
In order to elucidate how GREM1 contributes to hereditary mixed polyposis syndrome, R&D Systems Ephb2 antibody (R and D, AF467) was used in immunohistochemistry - paraffin section on mouse samples . Nat Med (2015) ncbi
goat polyclonal
  • immunocytochemistry; human
  • western blot; human
In order to identify Eph tyrosine kinase receptors as the substrates of tissue factor/coagulation factor VIIa, R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in immunocytochemistry on human samples and in western blot on human samples . J Biol Chem (2014) ncbi
goat polyclonal
  • immunocytochemistry; human; 1:10
In order to study cord blood as a source of circulating endothelial progenitor cells that can be directed towards specialized endothelial phenotypes, R&D Systems Ephb2 antibody (R&D Systems, AF467) was used in immunocytochemistry on human samples at 1:10. PLoS ONE (2014) ncbi
BD Biosciences
mouse monoclonal (16/ERK)
  • western blot; mouse; loading ...; fig 4a
BD Biosciences Ephb2 antibody (BD Transduction Laboratories, 16/ERK) was used in western blot on mouse samples (fig 4a). J Immunol (2019) ncbi
mouse monoclonal (16/ERK)
  • western blot; human; fig 2e
BD Biosciences Ephb2 antibody (BD Biosciences, 16) was used in western blot on human samples (fig 2e). Sci Rep (2018) ncbi
mouse monoclonal (16/ERK)
  • western blot; mouse; loading ...; fig 3e
In order to explore the role of Hic-5 in breast tumor progression, BD Biosciences Ephb2 antibody (BD Biosciences, 610124) was used in western blot on mouse samples (fig 3e). Oncogene (2017) ncbi
mouse monoclonal (16/ERK)
  • western blot; human; fig 6
BD Biosciences Ephb2 antibody (BD Transduction, 610123) was used in western blot on human samples (fig 6). PLoS ONE (2015) ncbi
mouse monoclonal (16/ERK)
  • western blot; human; fig 2
In order to show that fatty acylated cav-2 is a IR tyrosine kinase substrate during insulin signaling, BD Biosciences Ephb2 antibody (BD, 610124) was used in western blot on human samples (fig 2). Biochim Biophys Acta (2015) ncbi
mouse monoclonal (16/ERK)
  • western blot; mouse
BD Biosciences Ephb2 antibody (BD Biosciences, 610123) was used in western blot on mouse samples . Mol Cell Biol (2015) ncbi
mouse monoclonal (16/ERK)
  • western blot; human
In order to determine if CK1 has a role in Sprouty2 phosphorylation and function, BD Biosciences Ephb2 antibody (BD Transduction Laboratories, 610124) was used in western blot on human samples . Oncogene (2015) ncbi
mouse monoclonal (16/ERK)
  • western blot; human
BD Biosciences Ephb2 antibody (BD, 610123) was used in western blot on human samples . J Am Heart Assoc (2013) ncbi
Articles Reviewed
  1. Simonović N, Witalisz Siepracka A, Meissl K, Lassnig C, Reichart U, Kolbe T, et al. NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity. J Immunol. 2019;202:1724-1734 pubmed publisher
  2. Vanshylla K, Bartsch C, Hitzing C, Krümpelmann L, Wienands J, Engels N. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep. 2018;8:4244 pubmed publisher
  3. Goreczny G, Ouderkirk Pecone J, Olson E, Krendel M, Turner C. Hic-5 remodeling of the stromal matrix promotes breast tumor progression. Oncogene. 2017;36:2693-2703 pubmed publisher
  4. De Robertis M, Loiacono L, Fusilli C, Poeta M, Mazza T, Sanchez M, et al. Dysregulation of EGFR Pathway in EphA2 Cell Subpopulation Significantly Associates with Poor Prognosis in Colorectal Cancer. Clin Cancer Res. 2017;23:159-170 pubmed publisher
  5. Planaguma J, Haselmann H, Mannara F, Petit Pedrol M, Grünewald B, Aguilar E, et al. Ephrin-B2 prevents N-methyl-D-aspartate receptor antibody effects on memory and neuroplasticity. Ann Neurol. 2016;80:388-400 pubmed publisher
  6. Gong J, Körner R, Gaitanos L, Klein R. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol. 2016;214:35-44 pubmed publisher
  7. Herring A, Münster Y, Akkaya T, Moghaddam S, Deinsberger K, Meyer J, et al. Kallikrein-8 inhibition attenuates Alzheimer's disease pathology in mice. Alzheimers Dement. 2016;12:1273-1287 pubmed publisher
  8. Zhu X, Liu X, Sun S, Zhuang H, Yang J, Henkemeyer M, et al. Ephrin-B3 coordinates timed axon targeting and amygdala spinogenesis for innate fear behaviour. Nat Commun. 2016;7:11096 pubmed publisher
  9. Perez de Arce K, Schrod N, Metzbower S, Allgeyer E, Kong G, Tang A, et al. Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains. Neuron. 2015;88:1165-1172 pubmed publisher
  10. Miyamoto T, Kim D, Knox J, Johnson E, Mucke L. Increasing the Receptor Tyrosine Kinase EphB2 Prevents Amyloid-β-induced Depletion of Cell Surface Glutamate Receptors by a Mechanism That Requires the PDZ-binding Motif of EphB2 and Neuronal Activity. J Biol Chem. 2016;291:1719-34 pubmed publisher
  11. Oprea T, Sklar L, Agola J, Guo Y, Silberberg M, Roxby J, et al. Novel Activities of Select NSAID R-Enantiomers against Rac1 and Cdc42 GTPases. PLoS ONE. 2015;10:e0142182 pubmed publisher
  12. Jardé T, Kass L, Staples M, Lescesen H, Carne P, Oliva K, et al. ERBB3 Positively Correlates with Intestinal Stem Cell Markers but Marks a Distinct Non Proliferative Cell Population in Colorectal Cancer. PLoS ONE. 2015;10:e0138336 pubmed publisher
  13. Kwon H, Lee J, Jeong K, Jang D, Pak Y. Fatty acylated caveolin-2 is a substrate of insulin receptor tyrosine kinase for insulin receptor substrate-1-directed signaling activation. Biochim Biophys Acta. 2015;1853:1022-34 pubmed publisher
  14. Wiesauer I, Gaumannmüller C, Steinparzer I, Strobl B, Kovarik P. Promoter occupancy of STAT1 in interferon responses is regulated by processive transcription. Mol Cell Biol. 2015;35:716-27 pubmed publisher
  15. Davis H, Irshad S, Bansal M, Rafferty H, Boitsova T, Bardella C, et al. Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside the stem cell niche. Nat Med. 2015;21:62-70 pubmed publisher
  16. Eriksson O, Ramström M, Hörnaeus K, Bergquist J, Mokhtari D, Siegbahn A. The Eph tyrosine kinase receptors EphB2 and EphA2 are novel proteolytic substrates of tissue factor/coagulation factor VIIa. J Biol Chem. 2014;289:32379-91 pubmed publisher
  17. Yim D, Ghosh S, Guy G, Virshup D. Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling. Oncogene. 2015;34:474-84 pubmed publisher
  18. Boyer Di Ponio J, El Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE. 2014;9:e84179 pubmed publisher
  19. Xiong Y, Yu Y, Montani J, Yang Z, Ming X. Arginase-II induces vascular smooth muscle cell senescence and apoptosis through p66Shc and p53 independently of its l-arginine ureahydrolase activity: implications for atherosclerotic plaque vulnerability. J Am Heart Assoc. 2013;2:e000096 pubmed publisher