This is a Validated Antibody Database (VAD) review about mouse Eef2, based on 37 published articles (read how Labome selects the articles), using Eef2 antibody in all methods. It is aimed to help Labome visitors find the most suited Eef2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Eef2 synonym: Ef-2; elongation factor 2

Santa Cruz Biotechnology
mouse monoclonal (C-9)
  • western blot; mouse; 1:1000; fig S8
In order to research mitochondrial-targeted catalase good for the old mouse proteome but not the young which may function as a reverse antagonistic pleiotropy, Santa Cruz Biotechnology Eef2 antibody (Santa Cruz Biotechnology, sc-166415) was used in western blot on mouse samples at 1:1000 (fig S8). Aging Cell (2016) ncbi
mouse monoclonal (F-9)
  • western blot; human
In order to examine what proteins are produced when carbohydrate-fed healthy humans receive enteral delivery of proteins, Santa Cruz Biotechnology Eef2 antibody (Santa Cruz Biotechnology, sc-166409) was used in western blot on human samples . Am J Clin Nutr (2015) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; loading ...; fig 6a
In order to research the role of vacuolar-type H+-ATPase complex in liver in the accumulation of autophagic vacuoles and lysosomes, Abcam Eef2 antibody (Abcam, ab33523) was used in western blot on mouse samples (fig 6a). Autophagy (2017) ncbi
rabbit polyclonal
  • western blot; rat; 1:12500
Abcam Eef2 antibody (Abcam, ab40812) was used in western blot on rat samples at 1:12500. PLoS ONE (2014) ncbi
Bethyl
rabbit polyclonal
  • RNA immunoprecipitation; human
  • immunocytochemistry; human; 2 ug/ml
  • western blot; human; 0.2 ug/ml
In order to develop reagents to study RNA binding proteins, Bethyl Eef2 antibody (Bethyl, A301-688A) was used in RNA immunoprecipitation on human samples , in immunocytochemistry on human samples at 2 ug/ml and in western blot on human samples at 0.2 ug/ml. Mol Cell (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1c
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples at 1:1000 (fig 1c). Am J Physiol Endocrinol Metab (2019) ncbi
rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 11b
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on human samples (fig 11b). J Clin Invest (2018) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4a
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2331) was used in western blot on mouse samples at 1:1000 (fig 4a). J Neurosci (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4a
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples at 1:1000 (fig 4a). J Neurosci (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 2e
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples (fig 2e). J Inflamm (Lond) (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 1e
  • western blot; human; loading ...; fig 1f
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples (fig 1e) and in western blot on human samples (fig 1f). Oncotarget (2017) ncbi
rabbit polyclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology Eef2 antibody (CST, 2332) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1c
In order to discover that repeated exposure of striatal GABAergic spiny projecting neurons to D-amphetamine decreases global striatal mRNA translation, Cell Signaling Technology Eef2 antibody (Cell Signaling, 2331) was used in western blot on mouse samples at 1:1000 (fig 1c). Front Mol Neurosci (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 3
In order to test if repeated maximal-intensity hypoxic exercise induces beneficial adaptations on the hypoxia inducible factor-1alpha pathway when combined with chronic hypoxic exposure, Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on human samples (fig 3). Acta Physiol (Oxf) (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...
In order to analyze the context specificity of signaling networks within a causal conceptual framework using reverse-phase protein array time-course assays and network analysis approaches, Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2332) was used in western blot on human samples . Cell Syst (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 3c
In order to identify target genes of activating transcription factor 3 involved in muscle adaptation to training, Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples (fig 3c). FASEB J (2017) ncbi
rabbit polyclonal
  • western blot; rat; 1:3000; loading ...; fig 2a
Cell Signaling Technology Eef2 antibody (cell signalling, 2332) was used in western blot on rat samples at 1:3000 (fig 2a). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig 7a
In order to examine the composition of mitochondrial proteins in cases of fatal familial insomnia, Cell Signaling Technology Eef2 antibody (Cell signaling, 2332) was used in western blot on human samples at 1:500 (fig 7a). Brain Pathol (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 3
Cell Signaling Technology Eef2 antibody (Cell signaling, 2332) was used in western blot on human samples (fig 3). J Virol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4a
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples (fig 4a). Nature (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4a
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2331) was used in western blot on mouse samples (fig 4a). Nature (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1
Cell Signaling Technology Eef2 antibody (Cell Signaling Technolog, 2332) was used in western blot on mouse samples (fig 1). FASEB J (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 2
In order to assess the effect of intensive training in combination with marked reduction in training volume on phospholemman expression and phosphorylation at rest and during exercise, Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on human samples (fig 2). Am J Physiol Regul Integr Comp Physiol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 6
In order to study the coupling of cellular prion protein to intracellular signaling in Alzheimer's disease by metabotropic glutamate receptor 5, Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on mouse samples at 1:1000 (fig 6). Brain (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 6
In order to study the coupling of cellular prion protein to intracellular signaling in Alzheimer's disease by metabotropic glutamate receptor 5, Cell Signaling Technology Eef2 antibody (Cell Signalling, 2331) was used in western blot on mouse samples at 1:1000 (fig 6). Brain (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1
Cell Signaling Technology Eef2 antibody (Sigma-Aldrich, 2332) was used in western blot on mouse samples (fig 1). J Neurochem (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 6
Cell Signaling Technology Eef2 antibody (Cell Signaling Tech, 2332) was used in western blot on mouse samples at 1:1000 (fig 6). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 4b
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2331) was used in western blot on human samples (fig 4b). J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 4b
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2332) was used in western blot on human samples (fig 4b). J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2332) was used in western blot on human samples . Cell Signal (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on human samples . Cell Signal (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5
In order to study how acute environmental hypoxia regulates blood glucose and downstream intramuscular insulin signaling after humans eat a meal, Cell Signaling Technology Eef2 antibody (Cell signaling, 2332) was used in western blot on human samples (fig 5). Eur J Appl Physiol (2015) ncbi
rabbit polyclonal
  • western blot; mouse
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on mouse samples . J Proteomics (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on mouse samples at 1:1000. Nutr Res (2014) ncbi
rabbit polyclonal
  • western blot; mouse
In order to test if constitutive activation of SIRT1 in skeletal muscle prevents high fat diet-induced muscle insulin resistance, Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2332) was used in western blot on mouse samples . Am J Physiol Endocrinol Metab (2014) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on human samples . J Biol Chem (2014) ncbi
rabbit polyclonal
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2332) was used in immunoprecipitation on human samples , in immunocytochemistry on human samples and in western blot on human samples . J Biol Chem (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
  • western blot; rat; 1:1000; fig 2
  • western blot; human; 1:1000; fig 7
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2332) was used in western blot on mouse samples at 1:1000 (fig 5), in western blot on rat samples at 1:1000 (fig 2) and in western blot on human samples at 1:1000 (fig 7). Sci Rep (2013) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
  • western blot; rat; 1:1000; fig 2
  • western blot; human; 1:1000; fig 7
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on mouse samples at 1:1000 (fig 5), in western blot on rat samples at 1:1000 (fig 2) and in western blot on human samples at 1:1000 (fig 7). Sci Rep (2013) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4
Cell Signaling Technology Eef2 antibody (Cell Signaling Technology, 2331) was used in western blot on mouse samples (fig 4). J Physiol (2013) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology Eef2 antibody (Cell Signaling, 2331) was used in western blot on human samples (fig 1). J Physiol (2013) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; rat; 1:1000
Sigma-Aldrich Eef2 antibody (Sigma Aldrich, SAB4500695) was used in western blot on rat samples at 1:1000. Mol Psychiatry (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human
EMD Millipore Eef2 antibody (Millipore, 07-1382) was used in western blot on human samples . J Biol Chem (2014) ncbi
Articles Reviewed
  1. Martins V, Dent J, Svensson K, Tahvilian S, Begur M, Lakkaraju S, et al. Germline or inducible knockout of p300 or CBP in skeletal muscle does not alter insulin sensitivity. Am J Physiol Endocrinol Metab. 2019;316:E1024-E1035 pubmed publisher
  2. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  3. Chennupati V, Veiga D, Maslowski K, Andina N, Tardivel A, Yu E, et al. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation. J Clin Invest. 2018;128:1597-1614 pubmed publisher
  4. Salazar S, Gallardo C, Kaufman A, Herber C, Haas L, Robinson S, et al. Conditional Deletion of Prnp Rescues Behavioral and Synaptic Deficits after Disease Onset in Transgenic Alzheimer's Disease. J Neurosci. 2017;37:9207-9221 pubmed publisher
  5. Le N, Kim C, Tu T, Kim B, Park T, Park J, et al. Absence of 4-1BB reduces obesity-induced atrophic response in skeletal muscle. J Inflamm (Lond). 2017;14:9 pubmed publisher
  6. Lee T, Pelletier J. Dependence of p53-deficient cells on the DHX9 DExH-box helicase. Oncotarget. 2017;8:30908-30921 pubmed publisher
  7. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  8. Kissing S, Rudnik S, Damme M, Lüllmann Rauch R, Ichihara A, Kornak U, et al. Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes. Autophagy. 2017;13:670-685 pubmed publisher
  9. Biever A, Boubaker Vitre J, Cutando L, Gracia Rubio I, Costa Mattioli M, Puighermanal E, et al. Repeated Exposure to D-Amphetamine Decreases Global Protein Synthesis and Regulates the Translation of a Subset of mRNAs in the Striatum. Front Mol Neurosci. 2016;9:165 pubmed publisher
  10. Brocherie F, Millet G, D Hulst G, Van Thienen R, Deldicque L, Girard O. Repeated maximal-intensity hypoxic exercise superimposed to hypoxic residence boosts skeletal muscle transcriptional responses in elite team-sport athletes. Acta Physiol (Oxf). 2018;222: pubmed publisher
  11. Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
  12. Fernández Verdejo R, Vanwynsberghe A, Essaghir A, Demoulin J, Hai T, Deldicque L, et al. Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training. FASEB J. 2017;31:840-851 pubmed publisher
  13. Gupta S, Zeglinski M, Rattan S, Landry N, Ghavami S, Wigle J, et al. Inhibition of autophagy inhibits the conversion of cardiac fibroblasts to cardiac myofibroblasts. Oncotarget. 2016;7:78516-78531 pubmed publisher
  14. Frau Méndez M, Fernández Vega I, Ansoleaga B, Blanco Tech R, Carmona Tech M, Antonio Del Río J, et al. Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus. Brain Pathol. 2017;27:95-106 pubmed publisher
  15. Gilson T, Blanchette P, Ballmann M, Papp T, Pénzes J, Benkő M, et al. Using the E4orf6-Based E3 Ubiquitin Ligase as a Tool To Analyze the Evolution of Adenoviruses. J Virol. 2016;90:7350-7367 pubmed publisher
  16. Zanos P, Moaddel R, Morris P, Georgiou P, Fischell J, Elmer G, et al. NMDAR inhibition-independent antidepressant actions of ketamine metabolites. Nature. 2016;533:481-6 pubmed publisher
  17. Basisty N, Dai D, Gagnidze A, Gitari L, Fredrickson J, Maina Y, et al. Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?. Aging Cell. 2016;15:634-45 pubmed publisher
  18. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  19. Svensson K, Albert V, Cardel B, Salatino S, Handschin C. Skeletal muscle PGC-1α modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice. FASEB J. 2016;30:1976-86 pubmed publisher
  20. Thomassen M, Gunnarsson T, Christensen P, Pavlovic D, Shattock M, Bangsbo J. Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes. Am J Physiol Regul Integr Comp Physiol. 2016;310:R659-69 pubmed publisher
  21. Haas L, Salazar S, Kostylev M, Um J, Kaufman A, Strittmatter S. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2016;139:526-46 pubmed publisher
  22. Kenney J, Genheden M, Moon K, Wang X, Foster L, Proud C. Eukaryotic elongation factor 2 kinase regulates the synthesis of microtubule-related proteins in neurons. J Neurochem. 2016;136:276-84 pubmed publisher
  23. Goichon A, Bertrand J, Chan P, Lecleire S, Coquard A, Cailleux A, et al. Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa. Am J Clin Nutr. 2015;102:359-67 pubmed publisher
  24. Sato M, Matsubara T, Adachi J, Hashimoto Y, Fukamizu K, Kishida M, et al. Differential Proteome Analysis Identifies TGF-β-Related Pro-Metastatic Proteins in a 4T1 Murine Breast Cancer Model. PLoS ONE. 2015;10:e0126483 pubmed publisher
  25. Fonseca B, Zakaria C, Jia J, Graber T, Svitkin Y, Tahmasebi S, et al. La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1). J Biol Chem. 2015;290:15996-6020 pubmed publisher
  26. Wnorowski A, Sadowska M, Paul R, Singh N, Boguszewska Czubara A, Jimenez L, et al. Activation of β2-adrenergic receptor by (R,R')-4'-methoxy-1-naphthylfenoterol inhibits proliferation and motility of melanoma cells. Cell Signal. 2015;27:997-1007 pubmed publisher
  27. D Hulst G, Sylow L, Hespel P, Deldicque L. Acute systemic insulin intolerance does not alter the response of the Akt/GSK-3 pathway to environmental hypoxia in human skeletal muscle. Eur J Appl Physiol. 2015;115:1219-31 pubmed publisher
  28. Kettenbach A, Sano H, Keller S, Lienhard G, Gerber S. SPECHT - single-stage phosphopeptide enrichment and stable-isotope chemical tagging: quantitative phosphoproteomics of insulin action in muscle. J Proteomics. 2015;114:48-60 pubmed publisher
  29. Areta J, Hawley J, Ye J, Chan M, Coffey V. Increasing leucine concentration stimulates mechanistic target of rapamycin signaling and cell growth in C2C12 skeletal muscle cells. Nutr Res. 2014;34:1000-7 pubmed publisher
  30. Leal G, Afonso P, Duarte C. Neuronal activity induces synaptic delivery of hnRNP A2/B1 by a BDNF-dependent mechanism in cultured hippocampal neurons. PLoS ONE. 2014;9:e108175 pubmed publisher
  31. Ledonne A, Nobili A, Latagliata E, Cavallucci V, Guatteo E, Puglisi Allegra S, et al. Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons. Mol Psychiatry. 2015;20:959-73 pubmed publisher
  32. White A, Philp A, Fridolfsson H, Schilling J, Murphy A, Hamilton D, et al. High-fat diet-induced impairment of skeletal muscle insulin sensitivity is not prevented by SIRT1 overexpression. Am J Physiol Endocrinol Metab. 2014;307:E764-72 pubmed publisher
  33. Tavares C, Ferguson S, Giles D, Wang Q, Wellmann R, O Brien J, et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem. 2014;289:23901-16 pubmed publisher
  34. Yao Q, Liu B, Li H, McGarrigle D, Xing B, Zhou M, et al. C-terminal Src kinase (Csk)-mediated phosphorylation of eukaryotic elongation factor 2 (eEF2) promotes proteolytic cleavage and nuclear translocation of eEF2. J Biol Chem. 2014;289:12666-78 pubmed publisher
  35. Whittington R, Bretteville A, Virag L, Emala C, Maurin T, Marcouiller F, et al. Anesthesia-induced hypothermia mediates decreased ARC gene and protein expression through ERK/MAPK inactivation. Sci Rep. 2013;3:1388 pubmed publisher
  36. Alsted T, Ploug T, Prats C, Serup A, Høeg L, Schjerling P, et al. Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle. J Physiol. 2013;591:5141-55 pubmed publisher
  37. Areta J, Burke L, Ross M, Camera D, West D, Broad E, et al. Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis. J Physiol. 2013;591:2319-31 pubmed publisher