This is a Validated Antibody Database (VAD) review about mouse Drosha, based on 33 published articles (read how Labome selects the articles), using Drosha antibody in all methods. It is aimed to help Labome visitors find the most suited Drosha antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Drosha synonym: 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen; ribonuclease 3; RNase III; ethanol induced 2; protein Drosha; ribonuclease III, nuclear

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D28B1)
  • western blot knockout validation; mouse; loading ...; fig s3d
In order to study the function of DGCR8 in murine embryonic stem cells, Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot knockout validation on mouse samples (fig s3d). J Cell Biol (2017) ncbi
Abcam
rabbit polyclonal
  • immunocytochemistry; human; 1:250; fig 4
  • western blot; human; 1:1000; fig 2
In order to study the affects in the subcellular localization of Drosha by alternative splicing, Abcam Drosha antibody (Abcam, ab-12286) was used in immunocytochemistry on human samples at 1:250 (fig 4) and in western blot on human samples at 1:1000 (fig 2). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples at 1:1000. Nat Commun (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 3c
  • immunocytochemistry; human; loading ...; fig 3f
  • western blot; human; loading ...; fig 3b
Abcam Drosha antibody (Abcam, ab12286) was used in chromatin immunoprecipitation on human samples (fig 3c), in immunocytochemistry on human samples (fig 3f) and in western blot on human samples (fig 3b). Oncogene (2016) ncbi
rabbit polyclonal
  • western blot; scFv; fig s8
  • western blot; mouse; fig 7
Abcam Drosha antibody (AbCam, ab12286) was used in western blot on scFv samples (fig s8) and in western blot on mouse samples (fig 7). Autophagy (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:300
In order to describe a miRNA-dependent mechanism of Dicer in reestablishing the barrier function of the skin post-wounding, Abcam Drosha antibody (Abcam, ab12286) was used in western blot on mouse samples at 1:300. Mol Ther (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500
Abcam Drosha antibody (SigAbcam, Ab12286) was used in immunohistochemistry - paraffin section on human samples at 1:500. J Neuropathol Exp Neurol (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig s11
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples at 1:1000 (fig s11). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:25
  • western blot; human
In order to examine the generation and function of small RNAs from cytoplasmic RNA viruses, Abcam Drosha antibody (Abcam, ab12286) was used in immunocytochemistry on human samples at 1:25 and in western blot on human samples . Nucleic Acids Res (2014) ncbi
rabbit polyclonal
  • western blot; pig; 1:1000
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on pig samples at 1:1000. Anim Genet (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
Abcam Drosha antibody (Abcam, ab12286) was used in immunocytochemistry on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:500
Abcam Drosha antibody (Abcam, ab12286) was used in immunohistochemistry - paraffin section on mouse samples at 1:500. Biol Reprod (2014) ncbi
rabbit polyclonal
  • western blot; pig; 1:2000
In order to study the mechanisms underlying the effects of a maternal low-protein diet on offspring ovarian steroid synthesis and folliculogenesis, Abcam Drosha antibody (Abcam, ab12286) was used in western blot on pig samples at 1:2000. J Steroid Biochem Mol Biol (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples . PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; human; 1:400; fig 2
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples at 1:400 (fig 2). Blood Cancer J (2013) ncbi
rabbit polyclonal
  • western blot; human
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples . Mol Neurobiol (2013) ncbi
rabbit polyclonal
  • western blot; human
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
rabbit polyclonal
  • western blot; human; 1:400
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples at 1:400. Endocr Relat Cancer (2013) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples at 1:1000. J Biol Chem (2013) ncbi
rabbit polyclonal
  • western blot; human
In order to demonstrate the effect of HIV-1 Tat C on miRNA-101 expression in human brain microvascular endothelial cells, Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples . J Neurosci (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:50
Abcam Drosha antibody (Abcam, ab12286) was used in immunohistochemistry - paraffin section on human samples at 1:50. Mod Pathol (2013) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on mouse samples . Oncogene (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
  • western blot; human
Abcam Drosha antibody (Abcam, ab12286) was used in chromatin immunoprecipitation on human samples and in western blot on human samples . EMBO J (2012) ncbi
rabbit polyclonal
  • western blot; human; fig 3
Abcam Drosha antibody (Abcam, ab12286) was used in western blot on human samples (fig 3). Oncogene (2013) ncbi
Invitrogen
rabbit monoclonal (S.12.0)
  • western blot; human; loading ...; fig s3c
Invitrogen Drosha antibody (Thermo Fisher, MA5-14784) was used in western blot on human samples (fig s3c). Nucleic Acids Res (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot; human; loading ...; fig 6a
Santa Cruz Biotechnology Drosha antibody (Santa Cruz, sc-393591) was used in western blot on human samples (fig 6a). Sci Rep (2017) ncbi
Cell Signaling Technology
rabbit monoclonal (D28B1)
  • chromatin immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology Drosha antibody (CST, 3364) was used in chromatin immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 1b). Biochim Biophys Acta Gene Regul Mech (2017) ncbi
rabbit monoclonal (D28B1)
  • western blot knockout validation; mouse; loading ...; fig s3d
In order to study the function of DGCR8 in murine embryonic stem cells, Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot knockout validation on mouse samples (fig s3d). J Cell Biol (2017) ncbi
rabbit monoclonal (D28B1)
  • western blot; human; fig s3b
Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot on human samples (fig s3b). Nucleic Acids Res (2016) ncbi
rabbit monoclonal (D28B1)
  • western blot; human; 1:500; fig 3
In order to study the affects in the subcellular localization of Drosha by alternative splicing, Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:500 (fig 3). Nucleic Acids Res (2016) ncbi
rabbit monoclonal (D28B1)
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology Drosha antibody (Cell Signaling Tech, 3364) was used in western blot on mouse samples at 1:1000 (fig 2). Nat Commun (2016) ncbi
rabbit monoclonal (D28B1)
  • western blot; human; 1:1000; fig s1
In order to suggest that that DICER, DROSHA, and DDRNAs recruit DNA damage factors, Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:1000 (fig s1). J Cell Sci (2016) ncbi
rabbit monoclonal (D28B1)
  • western blot; human; 1:1000; fig 4
Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:1000 (fig 4). Nucleic Acids Res (2015) ncbi
rabbit monoclonal (D28B1)
  • western blot; human; 1:1000
Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot on human samples at 1:1000. Cell (2014) ncbi
rabbit monoclonal (D30F3)
  • western blot; human; 1:1000
Cell Signaling Technology Drosha antibody (Cell Signaling, 3410) was used in western blot on human samples at 1:1000. Cell (2014) ncbi
rabbit monoclonal (D28B1)
  • western blot; mouse; fig 5
Cell Signaling Technology Drosha antibody (Cell Signaling, 3364) was used in western blot on mouse samples (fig 5). J Biol Chem (2014) ncbi
Articles Reviewed
  1. Bonath F, Domingo Prim J, Tarbier M, Friedländer M, Visa N. Next-generation sequencing reveals two populations of damage-induced small RNAs at endogenous DNA double-strand breaks. Nucleic Acids Res. 2018;46:11869-11882 pubmed publisher
  2. Patne K, Rakesh R, Arya V, Chanana U, Sethy R, Swer P, et al. BRG1 and SMARCAL1 transcriptionally co-regulate DROSHA, DGCR8 and DICER in response to doxorubicin-induced DNA damage. Biochim Biophys Acta Gene Regul Mech. 2017;1860:936-951 pubmed publisher
  3. Dattilo V, D Antona L, Talarico C, Capula M, Catalogna G, Iuliano R, et al. SGK1 affects RAN/RANBP1/RANGAP1 via SP1 to play a critical role in pre-miRNA nuclear export: a new route of epigenomic regulation. Sci Rep. 2017;7:45361 pubmed publisher
  4. Cirera Salinas D, Yu J, Bodak M, Ngondo R, Herbert K, Ciaudo C. Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017;216:355-366 pubmed publisher
  5. Warner M, Bridge K, Hewitson J, Hodgkinson M, Heyam A, Massa B, et al. S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells. Nucleic Acids Res. 2016;44:9942-9955 pubmed
  6. Link S, Grund S, Diederichs S. Alternative splicing affects the subcellular localization of Drosha. Nucleic Acids Res. 2016;44:5330-43 pubmed publisher
  7. Salzman D, Nakamura K, Nallur S, Dookwah M, Metheetrairut C, Slack F, et al. miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nat Commun. 2016;7:10954 pubmed publisher
  8. Garibaldi F, Falcone E, Trisciuoglio D, Colombo T, Lisek K, Walerych D, et al. Mutant p53 inhibits miRNA biogenesis by interfering with the microprocessor complex. Oncogene. 2016;35:3760-70 pubmed publisher
  9. Murai K, Sun G, Ye P, Tian E, Yang S, Cui Q, et al. The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. Nat Commun. 2016;7:10965 pubmed publisher
  10. Francia S, Cabrini M, Matti V, Oldani A, d Adda di Fagagna F. DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors. J Cell Sci. 2016;129:1468-76 pubmed publisher
  11. Zhu C, Chen C, Huang J, Zhang H, Zhao X, Deng R, et al. SUMOylation at K707 of DGCR8 controls direct function of primary microRNA. Nucleic Acids Res. 2015;43:7945-60 pubmed publisher
  12. Kim Y, Kang Y, Lee N, Kim K, Hwang Y, Kim H, et al. Uvrag targeting by Mir125a and Mir351 modulates autophagy associated with Ewsr1 deficiency. Autophagy. 2015;11:796-811 pubmed publisher
  13. Ghatak S, Chan Y, Khanna S, Banerjee J, Weist J, Roy S, et al. Barrier Function of the Repaired Skin Is Disrupted Following Arrest of Dicer in Keratinocytes. Mol Ther. 2015;23:1201-1210 pubmed publisher
  14. Porta S, Kwong L, Trojanowski J, Lee V. Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases. J Neuropathol Exp Neurol. 2015;74:380-7 pubmed publisher
  15. Bahn J, Ahn J, Lin X, Zhang Q, Lee J, Civelek M, et al. Genomic analysis of ADAR1 binding and its involvement in multiple RNA processing pathways. Nat Commun. 2015;6:6355 pubmed publisher
  16. Weng K, Hung C, Hsieh P, Li M, Chen G, Kung Y, et al. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res. 2014;42:12789-805 pubmed publisher
  17. Li R, Jia Y, Zou H, Zhao R. Breed-specific expression of DROSHA, DICER and AGO2 is regulated by glucocorticoid-mediated miRNAs in the liver of newborn piglets. Anim Genet. 2014;45:817-26 pubmed publisher
  18. Bai B, Yegnasubramanian S, Wheelan S, Laiho M. RNA-Seq of the nucleolus reveals abundant SNORD44-derived small RNAs. PLoS ONE. 2014;9:e107519 pubmed publisher
  19. Yuan S, Ortogero N, Wu Q, Zheng H, Yan W. Murine follicular development requires oocyte DICER, but not DROSHA. Biol Reprod. 2014;91:39 pubmed publisher
  20. Sui S, He B, Jia Y, Li R, Cai D, Li X, et al. Maternal protein restriction during gestation and lactation programs offspring ovarian steroidogenesis and folliculogenesis in the prepubertal gilts. J Steroid Biochem Mol Biol. 2014;143:267-76 pubmed publisher
  21. Mori M, Triboulet R, Mohseni M, Schlegelmilch K, Shrestha K, Camargo F, et al. Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer. Cell. 2014;156:893-906 pubmed publisher
  22. Gurung B, Muhammad A, Hua X. Menin is required for optimal processing of the microRNA let-7a. J Biol Chem. 2014;289:9902-8 pubmed publisher
  23. Chen Y, Thang M, Chan Y, Huang Y, Ma N, Yu A, et al. Global assessment of Antrodia cinnamomea-induced microRNA alterations in hepatocarcinoma cells. PLoS ONE. 2013;8:e82751 pubmed publisher
  24. Caramuta S, Lee L, Ozata D, Akçakaya P, Georgii Hemming P, Xie H, et al. Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma. Blood Cancer J. 2013;3:e152 pubmed publisher
  25. Di Carlo V, Grossi E, Laneve P, Morlando M, Dini Modigliani S, Ballarino M, et al. TDP-43 regulates the microprocessor complex activity during in vitro neuronal differentiation. Mol Neurobiol. 2013;48:952-63 pubmed publisher
  26. Mattioli C, Pianigiani G, Pagani F. A competitive regulatory mechanism discriminates between juxtaposed splice sites and pri-miRNA structures. Nucleic Acids Res. 2013;41:8680-91 pubmed publisher
  27. Caramuta S, Lee L, Ozata D, Akçakaya P, Xie H, Höög A, et al. Clinical and functional impact of TARBP2 over-expression in adrenocortical carcinoma. Endocr Relat Cancer. 2013;20:551-64 pubmed publisher
  28. Narayanan A, Iordanskiy S, Das R, Van Duyne R, Santos S, Jaworski E, et al. Exosomes derived from HIV-1-infected cells contain trans-activation response element RNA. J Biol Chem. 2013;288:20014-33 pubmed publisher
  29. Mishra R, Singh S. HIV-1 Tat C modulates expression of miRNA-101 to suppress VE-cadherin in human brain microvascular endothelial cells. J Neurosci. 2013;33:5992-6000 pubmed publisher
  30. Jafarnejad S, Sjoestroem C, Martinka M, Li G. Expression of the RNase III enzyme DROSHA is reduced during progression of human cutaneous melanoma. Mod Pathol. 2013;26:902-10 pubmed publisher
  31. Kuchenreuther M, Weber J. The ARF tumor-suppressor controls Drosha translation to prevent Ras-driven transformation. Oncogene. 2014;33:300-7 pubmed publisher
  32. Morlando M, Dini Modigliani S, Torrelli G, Rosa A, Di Carlo V, Caffarelli E, et al. FUS stimulates microRNA biogenesis by facilitating co-transcriptional Drosha recruitment. EMBO J. 2012;31:4502-10 pubmed publisher
  33. Ballarino M, Jobert L, Dembele D, de la Grange P, Auboeuf D, Tora L. TAF15 is important for cellular proliferation and regulates the expression of a subset of cell cycle genes through miRNAs. Oncogene. 2013;32:4646-55 pubmed publisher