This is a Validated Antibody Database (VAD) review about mouse Dnmt3a, based on 33 published articles (read how Labome selects the articles), using Dnmt3a antibody in all methods. It is aimed to help Labome visitors find the most suited Dnmt3a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Dnmt3a synonym: MmuIIIA

Knockout validation
Abcam
domestic rabbit monoclonal (EPR18455)
  • western blot knockout validation; mouse; loading ...; fig e3a
Abcam Dnmt3a antibody (Abcam, ab188470) was used in western blot knockout validation on mouse samples (fig e3a). Nature (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D23G1)
  • immunocytochemistry knockout validation; mouse; 1:400; loading ...; fig 6c
  • western blot; human; 1:2000; loading ...; fig 1b
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, D23G1/3598) was used in immunocytochemistry knockout validation on mouse samples at 1:400 (fig 6c) and in western blot on human samples at 1:2000 (fig 1b). J Clin Invest (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:2000; fig 4b
  • western blot knockout validation; mouse; 1:2000; fig s1a
In order to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9, Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 2160) was used in western blot on human samples at 1:2000 (fig 4b) and in western blot knockout validation on mouse samples at 1:2000 (fig s1a). J Clin Invest (2016) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; fig 4a
Abcam Dnmt3a antibody (Abcam, ab2850) was used in western blot on human samples (fig 4a). Cell Death Dis (2021) ncbi
domestic rabbit polyclonal
  • RNA immunoprecipitation; human; 1:100; loading ...; fig 4e
  • chromatin immunoprecipitation; human; 1:100; loading ...; fig 4g
Abcam Dnmt3a antibody (Abcam, ab2850) was used in RNA immunoprecipitation on human samples at 1:100 (fig 4e) and in chromatin immunoprecipitation on human samples at 1:100 (fig 4g). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3b
Abcam Dnmt3a antibody (Abcam, ab2850) was used in western blot on human samples (fig 3b). Autophagy (2020) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; 1:50; loading ...; fig 3f
Abcam Dnmt3a antibody (Abcam, ab2850) was used in chromatin immunoprecipitation on human samples at 1:50 (fig 3f). Oncol Rep (2019) ncbi
domestic rabbit monoclonal (EPR18455)
  • western blot knockout validation; mouse; loading ...; fig e3a
Abcam Dnmt3a antibody (Abcam, ab188470) was used in western blot knockout validation on mouse samples (fig e3a). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4d
Abcam Dnmt3a antibody (Abcam, ab2850) was used in western blot on human samples (fig 4d). Onco Targets Ther (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; loading ...; fig 5c
Abcam Dnmt3a antibody (Abcam, ab2850) was used in immunohistochemistry - frozen section on mouse samples (fig 5c). J Cell Biol (2018) ncbi
domestic rabbit monoclonal (EPR18455)
  • western blot; mouse; loading ...; fig 2c
  • western blot; human; loading ...; fig 2a
Abcam Dnmt3a antibody (Abcam, ab188470) was used in western blot on mouse samples (fig 2c) and in western blot on human samples (fig 2a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 4a
Abcam Dnmt3a antibody (Abcam, ab2850) was used in western blot on mouse samples (fig 4a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 6d
  • western blot; mouse; 1:2000; loading ...; fig 6a
  • immunoprecipitation; human; loading ...; fig 3g
  • western blot; human; 1:2000; loading ...; fig 3b
Abcam Dnmt3a antibody (Abcam, ab2850) was used in chromatin immunoprecipitation on mouse samples (fig 6d), in western blot on mouse samples at 1:2000 (fig 6a), in immunoprecipitation on human samples (fig 3g) and in western blot on human samples at 1:2000 (fig 3b). Nucleic Acids Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s1
Abcam Dnmt3a antibody (abcam, ab2850) was used in western blot on human samples at 1:1000 (fig s1). Oncotarget (2016) ncbi
Novus Biologicals
mouse monoclonal (64B1446)
Novus Biologicals Dnmt3a antibody (Novus, NB120-13888) was used . elife (2020) ncbi
mouse monoclonal (64B1446)
  • immunocytochemistry; human; loading ...; fig 1a
In order to investigate the oncogenic functions of CHD4, Novus Biologicals Dnmt3a antibody (Novus Biologicals, NB120-13888) was used in immunocytochemistry on human samples (fig 1a). Cancer Cell (2017) ncbi
mouse monoclonal (64B1446)
  • immunocytochemistry; mouse
  • western blot; mouse
Novus Biologicals Dnmt3a antibody (Imgenex, 64B1446) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . Nucleic Acids Res (2015) ncbi
mouse monoclonal (64B1446)
  • western blot; human; fig 1
Novus Biologicals Dnmt3a antibody (Imgenex, clone 64B1446) was used in western blot on human samples (fig 1). Exp Cell Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-12)
  • western blot; human; 1:200; loading ...; fig 3a
Santa Cruz Biotechnology Dnmt3a antibody (Santa Cruz, sc-365769) was used in western blot on human samples at 1:200 (fig 3a). Oncol Rep (2019) ncbi
mouse monoclonal (64B1446)
  • immunohistochemistry - free floating section; rat; 1:500; loading ...; fig 4a
Santa Cruz Biotechnology Dnmt3a antibody (Santa Cruz Biotechnology, 64B1446) was used in immunohistochemistry - free floating section on rat samples at 1:500 (fig 4a). J Comp Neurol (2020) ncbi
mouse monoclonal (64B1446)
  • western blot; human
Santa Cruz Biotechnology Dnmt3a antibody (Santa Cruz Biotechnology, sc-52921) was used in western blot on human samples . Biochem Biophys Res Commun (2014) ncbi
Active Motif
mouse monoclonal (64B1446)
  • chromatin immunoprecipitation; human
Active Motif Dnmt3a antibody (Active motif, 39206) was used in chromatin immunoprecipitation on human samples . Biochem Pharmacol (2011) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D23G1)
  • immunohistochemistry; human; 1:100; fig 5f
  • western blot; human; 1:1500; loading ...; fig 3f
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 3598) was used in immunohistochemistry on human samples at 1:100 (fig 5f) and in western blot on human samples at 1:1500 (fig 3f). Mol Ther Oncolytics (2022) ncbi
domestic rabbit monoclonal (D23G1)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 3b
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, D23G1) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 3b). J Hematol Oncol (2021) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human; 1:1000; loading ...; fig 5a
Cell Signaling Technology Dnmt3a antibody (CST, 3598S) was used in western blot on human samples at 1:1000 (fig 5a). PLoS ONE (2021) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human; loading ...; fig 4i
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 3598) was used in western blot on human samples (fig 4i). Cancer Cell (2019) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; mouse; 1:1000; fig 3e
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, D23G1) was used in western blot on mouse samples at 1:1000 (fig 3e). Exp Hematol (2018) ncbi
domestic rabbit monoclonal (D23G1)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2a
Cell Signaling Technology Dnmt3a antibody (CST, 3598) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 2a). Sci Rep (2018) ncbi
domestic rabbit monoclonal (D23G1)
  • immunocytochemistry knockout validation; mouse; 1:400; loading ...; fig 6c
  • western blot; human; 1:2000; loading ...; fig 1b
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, D23G1/3598) was used in immunocytochemistry knockout validation on mouse samples at 1:400 (fig 6c) and in western blot on human samples at 1:2000 (fig 1b). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human; fig 4g
In order to understand the role of nicotinamide metabolism in the maintenance of glioblastoma stem cells, Cell Signaling Technology Dnmt3a antibody (Cell signaling, 3598) was used in western blot on human samples (fig 4g). JCI Insight (2017) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human; fig 4a
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 3598) was used in western blot on human samples (fig 4a). Biochem Biophys Res Commun (2017) ncbi
domestic rabbit monoclonal (D23G1)
  • immunoprecipitation; human; 1:50; fig 6b
In order to assess the ability of TGF-beta to alter DNA methyltransferase expression in primary lung fibroblasts, Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 3598) was used in immunoprecipitation on human samples at 1:50 (fig 6b). J Biol Chem (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:2000; fig 4b
  • western blot knockout validation; mouse; 1:2000; fig s1a
In order to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9, Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 2160) was used in western blot on human samples at 1:2000 (fig 4b) and in western blot knockout validation on mouse samples at 1:2000 (fig s1a). J Clin Invest (2016) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human; 1:500; loading ...; fig 1a, 2a
Cell Signaling Technology Dnmt3a antibody (Active Motif, 3598) was used in western blot on human samples at 1:500 (fig 1a, 2a). Oncotarget (2015) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human
Cell Signaling Technology Dnmt3a antibody (Cell signaling, D23G1) was used in western blot on human samples . Nucleic Acids Res (2014) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; mouse; 1:1000
Cell Signaling Technology Dnmt3a antibody (Cell Signaling Technology, 3598) was used in western blot on mouse samples at 1:1000. Neuroscience (2014) ncbi
domestic rabbit monoclonal (D23G1)
  • western blot; human; 1:3000
Cell Signaling Technology Dnmt3a antibody (Cell Signaling, 3598) was used in western blot on human samples at 1:3000. Exp Hematol (2012) ncbi
Articles Reviewed
  1. Huang Z, Tang Y, Shen Z, Yang K, Gao K. UXT, a novel DNMT3b-binding protein, promotes breast cancer progression via negatively modulating lncRNA MEG3/p53 axis. Mol Ther Oncolytics. 2022;24:497-506 pubmed publisher
  2. Yang S, Hollingshead M, Rubinstein L, Nguyen D, Larenjeira A, Kinders R, et al. TET2 and DNMT3A mutations and exceptional response to 4'-thio-2'-deoxycytidine in human solid tumor models. J Hematol Oncol. 2021;14:83 pubmed publisher
  3. Sternbach S, West N, Singhal N, Clements R, Basu S, Tripathi A, et al. The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PLoS ONE. 2021;16:e0250486 pubmed publisher
  4. Lee M, Nam H, Kang H, Lee W, Lee G, Sung G, et al. Epigenetic regulation of p62/SQSTM1 overcomes the radioresistance of head and neck cancer cells via autophagy-dependent senescence induction. Cell Death Dis. 2021;12:250 pubmed publisher
  5. Shao G, Fan X, Zhang P, Liu X, Huang L, Ji S. Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway. Aging (Albany NY). 2021;13:4962-4975 pubmed publisher
  6. González Rodríguez P, Cheray M, Füllgrabe J, Salli M, Engskog Vlachos P, Keane L, et al. The DNA methyltransferase DNMT3A contributes to autophagy long-term memory. Autophagy. 2020;:1-19 pubmed publisher
  7. Lavery L, Ure K, Wan Y, Luo C, Trostle A, Wang W, et al. Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome. elife. 2020;9: pubmed publisher
  8. Zhang L, Tian S, Pei M, Zhao M, Wang L, Jiang Y, et al. Crosstalk between histone modification and DNA methylation orchestrates the epigenetic regulation of the costimulatory factors, Tim‑3 and galectin‑9, in cervical cancer. Oncol Rep. 2019;42:2655-2669 pubmed publisher
  9. Weinberg D, Papillon Cavanagh S, Chen H, Yue Y, Chen X, Rajagopalan K, et al. The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape. Nature. 2019;573:281-286 pubmed publisher
  10. Pritchard R, Chen H, Romoli B, Spitzer N, Dulcis D. Photoperiod-induced neurotransmitter plasticity declines with aging: An epigenetic regulation?. J Comp Neurol. 2020;528:199-210 pubmed publisher
  11. Sin Chan P, Mumal I, Suwal T, Ho B, Fan X, Singh I, et al. A C19MC-LIN28A-MYCN Oncogenic Circuit Driven by Hijacked Super-enhancers Is a Distinct Therapeutic Vulnerability in ETMRs: A Lethal Brain Tumor. Cancer Cell. 2019;36:51-67.e7 pubmed publisher
  12. Wu R, Yun Q, Zhang J, Bao J. Downregulation of KLF13 through DNMT1-mediated hypermethylation promotes glioma cell proliferation and invasion. Onco Targets Ther. 2019;12:1509-1520 pubmed publisher
  13. Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  14. Shi X, Kitano A, Jiang Y, Luu V, Hoegenauer K, Nakada D. Clonal expansion and myeloid leukemia progression modeled by multiplex gene editing of murine hematopoietic progenitor cells. Exp Hematol. 2018;64:33-44.e5 pubmed publisher
  15. Gong Z, Zhou Q. Dnmt3a in the dorsal dentate gyrus is a key regulator of fear renewal. Sci Rep. 2018;8:5093 pubmed publisher
  16. Cole C, Russler Germain D, Ketkar S, Verdoni A, Smith A, Bangert C, et al. Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies. J Clin Invest. 2017;127:3657-3674 pubmed publisher
  17. Jung J, Kim L, Wang X, Wu Q, Sanvoranart T, Hubert C, et al. Nicotinamide metabolism regulates glioblastoma stem cell maintenance. JCI Insight. 2017;2: pubmed publisher
  18. Xia L, Huang W, Bellani M, Seidman M, Wu K, Fan D, et al. CHD4 Has Oncogenic Functions in Initiating and Maintaining Epigenetic Suppression of Multiple Tumor Suppressor Genes. Cancer Cell. 2017;31:653-668.e7 pubmed publisher
  19. Deivendran S, Marzook H, Santhoshkumar T, Kumar R, Pillai M. Metastasis-associated protein 1 is an upstream regulator of DNMT3a and stimulator of insulin-growth factor binding protein-3 in breast cancer. Sci Rep. 2017;7:44225 pubmed publisher
  20. Raciti G, Spinelli R, Desiderio A, Longo M, Parrillo L, Nigro C, et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep. 2017;7:43526 pubmed publisher
  21. Chen S, Jing Y, Kang X, Yang L, Wang D, Zhang W, et al. Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy. Nucleic Acids Res. 2017;45:1144-1158 pubmed publisher
  22. Du M, Zhang Y, Mao Y, Mou J, Zhao J, Xue Q, et al. MiR-33a suppresses proliferation of NSCLC cells via targeting METTL3 mRNA. Biochem Biophys Res Commun. 2017;482:582-589 pubmed publisher
  23. Koh H, Scruggs A, Huang S. Transforming Growth Factor-β1 Increases DNA Methyltransferase 1 and 3a Expression through Distinct Post-transcriptional Mechanisms in Lung Fibroblasts. J Biol Chem. 2016;291:19287-98 pubmed publisher
  24. Song G, Shi L, Guo Y, Yu L, Wang L, Zhang X, et al. A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells. Oncotarget. 2016;7:3144-57 pubmed publisher
  25. Cole C, Verdoni A, Ketkar S, Leight E, Russler Germain D, Lamprecht T, et al. PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia. J Clin Invest. 2016;126:85-98 pubmed publisher
  26. Mulholland C, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, et al. A modular open platform for systematic functional studies under physiological conditions. Nucleic Acids Res. 2015;43:e112 pubmed publisher
  27. Vispé S, Deroide A, Davoine E, Desjobert C, Lestienne F, Fournier L, et al. Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells. Oncotarget. 2015;6:15265-82 pubmed
  28. Huang H, Hu G, Cai J, Xia B, Liu J, Li X, et al. Role of poly(ADP-ribose) glycohydrolase silencing in DNA hypomethylation induced by benzo(a)pyrene. Biochem Biophys Res Commun. 2014;452:708-14 pubmed publisher
  29. Cho S, Park J, Kang Y. AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene. Nucleic Acids Res. 2014;42:13545-56 pubmed publisher
  30. Wongtrakoongate P, Li J, Andrews P. DNMT3B inhibits the re-expression of genes associated with induced pluripotency. Exp Cell Res. 2014;321:231-9 pubmed publisher
  31. Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  32. Valdez B, Nieto Y, Murray D, Li Y, Wang G, Champlin R, et al. Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines. Exp Hematol. 2012;40:800-10 pubmed publisher
  33. Karius T, Schnekenburger M, Ghelfi J, Walter J, Dicato M, Diederich M. Reversible epigenetic fingerprint-mediated glutathione-S-transferase P1 gene silencing in human leukemia cell lines. Biochem Pharmacol. 2011;81:1329-42 pubmed publisher