This is a Validated Antibody Database (VAD) review about mouse Dctn1, based on 28 published articles (read how Labome selects the articles), using Dctn1 antibody in all methods. It is aimed to help Labome visitors find the most suited Dctn1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Dctn1 synonym: AL022633; DAP-150; DP-150; Glued; p150; p150-glued; p150; dynactin subunit 1; 150 kDa dynein-associated polypeptide; dynactin 1, retrograde axonal transport

Abcam
goat polyclonal
  • western blot; human; fig s2
Abcam Dctn1 antibody (Abcam, Ab11806) was used in western blot on human samples (fig s2). J Cell Biol (2015) ncbi
BD Biosciences
mouse monoclonal (1/p150Glued)
  • immunohistochemistry; human; 1:500; loading ...; fig 1b
  • western blot; human; 1:5000; loading ...; fig s1m
BD Biosciences Dctn1 antibody (BDBiosciences, 610474) was used in immunohistochemistry on human samples at 1:500 (fig 1b) and in western blot on human samples at 1:5000 (fig s1m). Cell Rep (2018) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; loading ...; fig s3c
BD Biosciences Dctn1 antibody (BD, 610474) was used in immunocytochemistry on human samples (fig s3c). FEBS Lett (2017) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; 1:100; loading ...; fig s2c
In order to investigate that role of an ASPM-katanin complex in microtubule and spindle pole integrity, BD Biosciences Dctn1 antibody (BD Biosciences, 610474) was used in immunocytochemistry on human samples at 1:100 (fig s2c). Nat Cell Biol (2017) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; mouse; loading ...; fig 4I
In order to find that CNS-specific snx6-knockout mice exhibit deficits in spatial learning and memory, accompanied with loss of spines from distal dendrites of hippocampal CA1 pyramidal cells, BD Biosciences Dctn1 antibody (BD Biosciences, 610474) was used in western blot on mouse samples (fig 4I). elife (2017) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; 1:100; loading ...; fig 4c
In order to identify MAPKBP1 mutations as a genetic cause of juvenile or late-onset and cilia-independent nephronophthisis, BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in immunocytochemistry on human samples at 1:100 (fig 4c). Am J Hum Genet (2017) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; mouse; 1:1000; fig 1b
BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 610474) was used in western blot on mouse samples at 1:1000 (fig 1b). Nat Commun (2016) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; mouse; fig 7A
  • immunoprecipitation; human; loading ...; fig 6H
  • immunocytochemistry; human; loading ...; fig 6B
  • western blot; human; loading ...; fig 6C
In order to study the function of nuclear distribution E homolog 1, dynactin, and dynein complexes, BD Biosciences Dctn1 antibody (BD, 610474) was used in western blot on mouse samples (fig 7A), in immunoprecipitation on human samples (fig 6H), in immunocytochemistry on human samples (fig 6B) and in western blot on human samples (fig 6C). J Immunol (2016) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; loading ...
In order to report a role for MICAL3 in organizing midbody-associated protein complexes, BD Biosciences Dctn1 antibody (BD Biosciences, 610474) was used in immunocytochemistry on human samples . J Biol Chem (2016) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; African green monkey; fig 2
BD Biosciences Dctn1 antibody (BD, 610473) was used in western blot on African green monkey samples (fig 2). Nat Commun (2016) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; 1:100; loading ...; fig s4a
BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in immunocytochemistry on human samples at 1:100 (fig s4a). Nat Commun (2016) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; human; fig 5
BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 610473) was used in western blot on human samples (fig 5). Mol Cancer (2016) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; mouse; 1:300-1:500; fig s5g
  • western blot; mouse; 1:500; fig 7b
BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in immunocytochemistry on mouse samples at 1:300-1:500 (fig s5g) and in western blot on mouse samples at 1:500 (fig 7b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; human; 1:2000; fig 1
BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in western blot on human samples at 1:2000 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; 1:100; loading ...; fig 3b
  • western blot; human; 1:1000; loading ...; fig s1c
BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 610474) was used in immunocytochemistry on human samples at 1:100 (fig 3b) and in western blot on human samples at 1:1000 (fig s1c). PLoS ONE (2016) ncbi
mouse monoclonal (1/p150Glued)
  • proximity ligation assay; human; fig 7
  • immunocytochemistry; human; fig 6
BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in proximity ligation assay on human samples (fig 7) and in immunocytochemistry on human samples (fig 6). J Cell Biol (2015) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; dog; 1:500; fig 4a
BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in immunocytochemistry on dog samples at 1:500 (fig 4a). Nat Commun (2015) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; mouse; 1:1000; fig 4
BD Biosciences Dctn1 antibody (BD, 610473) was used in immunocytochemistry on mouse samples at 1:1000 (fig 4). Mol Biol Cell (2015) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; mouse
  • western blot; mouse
BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . J Biol Chem (2015) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; human; fig 2
BD Biosciences Dctn1 antibody (BD, 610473) was used in western blot on human samples (fig 2). J Cell Sci (2014) ncbi
mouse monoclonal (25/Dynactin p50)
  • western blot; human; fig 2
BD Biosciences Dctn1 antibody (BD, 611003) was used in western blot on human samples (fig 2). J Cell Sci (2014) ncbi
mouse monoclonal (1/p150Glued)
  • immunocytochemistry; human; 1:100
In order to assess the role of the anaphase-promoting complex and Cdc20 in the primary cilium, BD Biosciences Dctn1 antibody (BD Biosciences, 610473) was used in immunocytochemistry on human samples at 1:100. elife (2014) ncbi
mouse monoclonal (12/P150GLUED)
  • immunocytochemistry; human; 1:200
BD Biosciences Dctn1 antibody (Transduction Laboratories, 612709) was used in immunocytochemistry on human samples at 1:200. EMBO J (2014) ncbi
mouse monoclonal (25/Dynactin p50)
  • western blot; African green monkey
In order to study the binding of dynamitin to Arp1 and its role in dynactin integrity, BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 611002) was used in western blot on African green monkey samples . Mol Biol Cell (2014) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; African green monkey
In order to study the binding of dynamitin to Arp1 and its role in dynactin integrity, BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 610473) was used in western blot on African green monkey samples . Mol Biol Cell (2014) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; human; 1:5000
In order to study the role of p150glued in apoptosis, BD Biosciences Dctn1 antibody (BD, 610473) was used in western blot on human samples at 1:5000. PLoS ONE (2014) ncbi
mouse monoclonal (25/Dynactin p50)
  • western blot; rat
BD Biosciences Dctn1 antibody (BD Biosciences, 25) was used in western blot on rat samples . Neuroscience (2014) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; mouse
In order to investigate the role of huntingtin in the the regulation of autophagosome dynamics, BD Biosciences Dctn1 antibody (BD Biosciences, 610474) was used in western blot on mouse samples . J Neurosci (2014) ncbi
mouse monoclonal (25/Dynactin p50)
  • western blot; African green monkey
  • western blot; mouse
BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 611003) was used in western blot on African green monkey samples and in western blot on mouse samples . J Biol Chem (2013) ncbi
mouse monoclonal (1/p150Glued)
  • western blot; mouse; fig 3
  • western blot; African green monkey
BD Biosciences Dctn1 antibody (BD Transduction Laboratories, 610474) was used in western blot on mouse samples (fig 3) and in western blot on African green monkey samples . J Biol Chem (2013) ncbi
EMD Millipore
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 5a
In order to test if the beta-amyloid peptide disrupts microtubules, EMD Millipore Dctn1 antibody (Millipore, AB6048) was used in immunohistochemistry on mouse samples at 1:200 (fig 5a). Acta Neuropathol (2016) ncbi
Articles Reviewed
  1. Mönnich M, Borgeskov L, Breslin L, Jakobsen L, Rogowski M, Doğanlı C, et al. CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium. Cell Rep. 2018;22:2584-2592 pubmed publisher
  2. Wei J, Xu H, Meng W. Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk. FEBS Lett. 2017;591:2379-2393 pubmed publisher
  3. Jiang K, Rezabkova L, Hua S, Liu Q, Capitani G, Altelaar A, et al. Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nat Cell Biol. 2017;19:480-492 pubmed publisher
  4. Niu Y, Dai Z, Liu W, Zhang C, Yang Y, Guo Z, et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. elife. 2017;6: pubmed publisher
  5. Macia M, Halbritter J, Delous M, Bredrup C, Gutter A, Filhol E, et al. Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis. Am J Hum Genet. 2017;100:323-333 pubmed publisher
  6. Hinckelmann M, Virlogeux A, Niehage C, Poujol C, Choquet D, Hoflack B, et al. Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport. Nat Commun. 2016;7:13233 pubmed publisher
  7. Nath S, Christian L, Tan S, Ki S, Ehrlich L, Poenie M. Dynein Separately Partners with NDE1 and Dynactin To Orchestrate T Cell Focused Secretion. J Immunol. 2016;197:2090-101 pubmed publisher
  8. Liu Q, Liu F, Yu K, Tas R, Grigoriev I, Remmelzwaal S, et al. MICAL3 Flavoprotein Monooxygenase Forms a Complex with Centralspindlin and Regulates Cytokinesis. J Biol Chem. 2016;291:20617-29 pubmed publisher
  9. Reddy B, Mattson M, Wynne C, Vadpey O, Durra A, Chapman D, et al. Load-induced enhancement of Dynein force production by LIS1-NudE in vivo and in vitro. Nat Commun. 2016;7:12259 pubmed publisher
  10. Wijdeven R, Janssen H, Nahidiazar L, Janssen L, Jalink K, Berlin I, et al. Cholesterol and ORP1L-mediated ER contact sites control autophagosome transport and fusion with the endocytic pathway. Nat Commun. 2016;7:11808 pubmed publisher
  11. Andersen A, Flinck M, Oernbo E, Pedersen N, Viuff B, Pedersen S. Roles of acid-extruding ion transporters in regulation of breast cancer cell growth in a 3-dimensional microenvironment. Mol Cancer. 2016;15:45 pubmed publisher
  12. Itoh Y, Higuchi M, Oishi K, Kishi Y, Okazaki T, Sakai H, et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A. 2016;113:E2955-64 pubmed publisher
  13. Sadleir K, Kandalepas P, Buggia Prevot V, Nicholson D, Thinakaran G, Vassar R. Presynaptic dystrophic neurites surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer's disease. Acta Neuropathol. 2016;132:235-56 pubmed publisher
  14. Chavali P, Chandrasekaran G, Barr A, Tátrai P, Taylor C, Papachristou E, et al. A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun. 2016;7:11005 pubmed publisher
  15. Lopes C, Aubert S, Bourgois Rocha F, Barnat M, Rego A, Déglon N, et al. Dominant-Negative Effects of Adult-Onset Huntingtin Mutations Alter the Division of Human Embryonic Stem Cells-Derived Neural Cells. PLoS ONE. 2016;11:e0148680 pubmed publisher
  16. Dumas A, Lê Bury G, Marie Anaïs F, Herit F, Mazzolini J, Guilbert T, et al. The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking. J Cell Biol. 2015;211:359-72 pubmed publisher
  17. Jovasevic V, Naghavi M, Walsh D. Microtubule plus end-associated CLIP-170 initiates HSV-1 retrograde transport in primary human cells. J Cell Biol. 2015;211:323-37 pubmed publisher
  18. Tuncay H, Brinkmann B, Steinbacher T, Schürmann A, Gerke V, Iden S, et al. JAM-A regulates cortical dynein localization through Cdc42 to control planar spindle orientation during mitosis. Nat Commun. 2015;6:8128 pubmed publisher
  19. Guo Y, Zheng Y. Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell. 2015;26:3379-89 pubmed publisher
  20. Song W, Cho Y, Watt D, Cavalli V. Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated α-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration. J Biol Chem. 2015;290:14765-75 pubmed publisher
  21. Asante D, Stevenson N, Stephens D. Subunit composition of the human cytoplasmic dynein-2 complex. J Cell Sci. 2014;127:4774-87 pubmed publisher
  22. Wang W, Wu T, Kirschner M. The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium. elife. 2014;3:e03083 pubmed publisher
  23. Kotak S, Busso C, GONCZY P. NuMA interacts with phosphoinositides and links the mitotic spindle with the plasma membrane. EMBO J. 2014;33:1815-30 pubmed publisher
  24. Cheong F, Feng L, Sarkeshik A, Yates J, Schroer T. Dynactin integrity depends upon direct binding of dynamitin to Arp1. Mol Biol Cell. 2014;25:2171-80 pubmed publisher
  25. Ishikawa K, Saiki S, Furuya N, Yamada D, Imamichi Y, Li Y, et al. P150glued-associated disorders are caused by activation of intrinsic apoptotic pathway. PLoS ONE. 2014;9:e94645 pubmed publisher
  26. Han X, Cheng D, Song F, Zeng T, An L, Xie K. Decelerated transport and its mechanism of 2,5-hexanedione on middle-molecular-weight neurofilament in rat dorsal root ganglia cells. Neuroscience. 2014;269:192-8 pubmed publisher
  27. Wong Y, Holzbaur E. The regulation of autophagosome dynamics by huntingtin and HAP1 is disrupted by expression of mutant huntingtin, leading to defective cargo degradation. J Neurosci. 2014;34:1293-305 pubmed publisher
  28. Perlson E, Hendricks A, Lazarus J, Ben Yaakov K, Gradus T, Tokito M, et al. Dynein interacts with the neural cell adhesion molecule (NCAM180) to tether dynamic microtubules and maintain synaptic density in cortical neurons. J Biol Chem. 2013;288:27812-24 pubmed publisher