This is a Validated Antibody Database (VAD) review about mouse Dcaf1, based on 3 published articles (read how Labome selects the articles), using Dcaf1 antibody in all methods. It is aimed to help Labome visitors find the most suited Dcaf1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Dcaf1 synonym: AI447437; B930007L02Rik; Vprbp; mKIAA0800; DDB1- and CUL4-associated factor 1; protein VPRBP; Vpr (HIV-1) binding protein; serine/threonine-protein kinase VPRBP

Knockout validation
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; 1:2000; loading ...; fig s5b
  • western blot knockout validation; mouse; 1:2000; loading ...; fig 2c
  • immunoprecipitation; mouse; 1:200; loading ...; fig 6b
In order to study the role of DCAF1 in T-cell function through p53-dependent and -independent mechanisms, Proteintech Group Dcaf1 antibody (Proteintech, 11612-1-AP) was used in western blot knockout validation on human samples at 1:2000 (fig s5b), in western blot knockout validation on mouse samples at 1:2000 (fig 2c) and in immunoprecipitation on mouse samples at 1:200 (fig 6b). Nat Commun (2016) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:200; fig s1
  • western blot knockout validation; mouse; 1:1000; fig 3
Proteintech Group Dcaf1 antibody (Proteintech, 11612-1-AP) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig s1) and in western blot knockout validation on mouse samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
Proteintech Group
rabbit polyclonal
  • western blot knockout validation; human; 1:2000; loading ...; fig s5b
  • western blot knockout validation; mouse; 1:2000; loading ...; fig 2c
  • immunoprecipitation; mouse; 1:200; loading ...; fig 6b
In order to study the role of DCAF1 in T-cell function through p53-dependent and -independent mechanisms, Proteintech Group Dcaf1 antibody (Proteintech, 11612-1-AP) was used in western blot knockout validation on human samples at 1:2000 (fig s5b), in western blot knockout validation on mouse samples at 1:2000 (fig 2c) and in immunoprecipitation on mouse samples at 1:200 (fig 6b). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:200; fig s1
  • western blot knockout validation; mouse; 1:1000; fig 3
Proteintech Group Dcaf1 antibody (Proteintech, 11612-1-AP) was used in immunohistochemistry knockout validation on mouse samples at 1:200 (fig s1) and in western blot knockout validation on mouse samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:150
In order to evaluate diagnostic markers for lung cancer with bronchial brushing specimens, Proteintech Group Dcaf1 antibody (Proteintech Group, 11612-1-AP) was used in immunocytochemistry on human samples at 1:150. Cancer Cytopathol (2014) ncbi
Articles Reviewed
  1. Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, et al. DCAF1 controls T-cell function via p53-dependent and -independent mechanisms. Nat Commun. 2016;7:10307 pubmed publisher
  2. Yu C, Ji S, Sha Q, Sun Q, Fan H. CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturation. Nat Commun. 2015;6:8017 pubmed publisher
  3. Liu Y, Jiang Y, Wang B, Hao J, Shang L, Zhang T, et al. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer Cytopathol. 2014;122:833-41 pubmed publisher