This is a Validated Antibody Database (VAD) review about mouse Clec7a, based on 14 published articles (read how Labome selects the articles), using Clec7a antibody in all methods. It is aimed to help Labome visitors find the most suited Clec7a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Clec7a synonym: BGR; Clecsf12; beta-GR

Knockout validation
Abcam
rat monoclonal (2A11)
  • immunohistochemistry knockout validation; mouse; 20 ug/ml; loading ...; fig s1a
  • immunohistochemistry - frozen section; mouse; 20 ug/ml; loading ...; fig s1a
  • flow cytometry; mouse; loading ...; fig 1b
Abcam Clec7a antibody (Abcam, 2A11) was used in immunohistochemistry knockout validation on mouse samples at 20 ug/ml (fig s1a), in immunohistochemistry - frozen section on mouse samples at 20 ug/ml (fig s1a) and in flow cytometry on mouse samples (fig 1b). Nat Med (2017) ncbi
BioLegend
rat monoclonal (RH1)
  • flow cytometry; mouse; 1:200; loading ...; fig 3a
BioLegend Clec7a antibody (BioLegend, 144303) was used in flow cytometry on mouse samples at 1:200 (fig 3a). elife (2020) ncbi
rat monoclonal (RH1)
  • flow cytometry; mouse; 1:200; loading ...; fig 2a
BioLegend Clec7a antibody (BioLegend, 144305) was used in flow cytometry on mouse samples at 1:200 (fig 2a). Sci Adv (2019) ncbi
rat monoclonal (RH1)
  • immunohistochemistry - free floating section; mouse; loading ...; fig 6e
  • flow cytometry; mouse; 1:50; loading ...; fig 6f
BioLegend Clec7a antibody (BioLegend, 144302) was used in immunohistochemistry - free floating section on mouse samples (fig 6e) and in flow cytometry on mouse samples at 1:50 (fig 6f). Neuron (2019) ncbi
rat monoclonal (RH1)
  • flow cytometry; mouse; 1:100; loading ...; fig 4a
BioLegend Clec7a antibody (Biolegend, RH1) was used in flow cytometry on mouse samples at 1:100 (fig 4a). Front Immunol (2018) ncbi
rat monoclonal (RH1)
  • flow cytometry; mouse; fig 2
BioLegend Clec7a antibody (Biolegend, 144304) was used in flow cytometry on mouse samples (fig 2). Nat Commun (2016) ncbi
Bio-Rad
rat monoclonal (2A11)
  • flow cytometry; mouse; loading ...; fig 1d
Bio-Rad Clec7a antibody (Bio-Rad, 2A11) was used in flow cytometry on mouse samples (fig 1d). Science (2018) ncbi
rat monoclonal (2A11)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s3a
  • immunohistochemistry - paraffin section; human; loading ...; fig s3c
Bio-Rad Clec7a antibody (BioRad, MCA2289FT) was used in immunohistochemistry - paraffin section on mouse samples (fig s3a) and in immunohistochemistry - paraffin section on human samples (fig s3c). J Clin Invest (2017) ncbi
rat monoclonal (2A11)
  • flow cytometry; mouse; 1:100; fig s11
In order to report that autophaghy regulates NFkapaB activity through A20 sequestration, Bio-Rad Clec7a antibody (AbD, 2A11) was used in flow cytometry on mouse samples at 1:100 (fig s11). Nat Commun (2015) ncbi
rat monoclonal (2A11)
  • western blot; mouse; tbl 3
Bio-Rad Clec7a antibody (AbD Serotec, MCA2289T) was used in western blot on mouse samples (tbl 3). J Neuroimmune Pharmacol (2009) ncbi
Santa Cruz Biotechnology
rat monoclonal (15Y9)
  • western blot; mouse; tbl 3
Santa Cruz Biotechnology Clec7a antibody (Santa Cruz, sc-73897) was used in western blot on mouse samples (tbl 3). J Neuroimmune Pharmacol (2009) ncbi
Invitrogen
rat monoclonal (bg1fpj)
  • flow cytometry; mouse; 1:100; loading ...; fig S5
In order to find that C-type lectin dendritic cell immunoreceptor is required to modulate lung inflammation and bacterial burden in tuberculosis, Invitrogen Clec7a antibody (eBioscience, bg1fpj) was used in flow cytometry on mouse samples at 1:100 (fig S5). Proc Natl Acad Sci U S A (2017) ncbi
Abcam
rat monoclonal (2A11)
  • immunohistochemistry knockout validation; mouse; 20 ug/ml; loading ...; fig s1a
  • immunohistochemistry - frozen section; mouse; 20 ug/ml; loading ...; fig s1a
  • flow cytometry; mouse; loading ...; fig 1b
Abcam Clec7a antibody (Abcam, 2A11) was used in immunohistochemistry knockout validation on mouse samples at 20 ug/ml (fig s1a), in immunohistochemistry - frozen section on mouse samples at 20 ug/ml (fig s1a) and in flow cytometry on mouse samples (fig 1b). Nat Med (2017) ncbi
InvivoGen
rat monoclonal (R1-8g7)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s2a
  • immunohistochemistry; mouse; 1:50; loading ...; fig 1b
InvivoGen Clec7a antibody (InvivoGen, Mabg-mdect) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig s2a) and in immunohistochemistry on mouse samples at 1:50 (fig 1b). Acta Neuropathol Commun (2022) ncbi
rat monoclonal (R1-8g7)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4a
InvivoGen Clec7a antibody (InvivoGen, mabg-mdect) was used in immunohistochemistry - frozen section on mouse samples (fig 4a). Neuron (2021) ncbi
rat monoclonal (R1-8g7)
  • immunohistochemistry - free floating section; mouse; 1:50; loading ...; fig 5
InvivoGen Clec7a antibody (Invivogen, mabg-mdect) was used in immunohistochemistry - free floating section on mouse samples at 1:50 (fig 5). elife (2020) ncbi
Articles Reviewed
  1. Shi H, Yin Z, Koronyo Y, Fuchs D, Sheyn J, Davis M, et al. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia. Acta Neuropathol Commun. 2022;10:136 pubmed publisher
  2. Safaiyan S, Besson Girard S, Kaya T, Cantuti Castelvetri L, Liu L, Ji H, et al. White matter aging drives microglial diversity. Neuron. 2021;109:1100-1117.e10 pubmed publisher
  3. Sebastian Monasor L, Müller S, Colombo A, Tanrioever G, König J, Roth S, et al. Fibrillar Aβ triggers microglial proteome alterations and dysfunction in Alzheimer mouse models. elife. 2020;9: pubmed publisher
  4. Lee J, Zhang J, Chung Y, Kim J, Kook C, Gonzalez Navajas J, et al. Inhibition of IRF4 in dendritic cells by PRR-independent and -dependent signals inhibit Th2 and promote Th17 responses. elife. 2020;9: pubmed publisher
  5. Geng S, Zhang Y, Lee C, Li L. Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis. Sci Adv. 2019;5:eaav2309 pubmed publisher
  6. Li Q, Cheng Z, Zhou L, Darmanis S, Neff N, Okamoto J, et al. Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing. Neuron. 2019;101:207-223.e10 pubmed publisher
  7. Zhu Y, Zhou J, Feng Y, Chen L, Zhang L, Yang F, et al. Control of Intestinal Inflammation, Colitis-Associated Tumorigenesis, and Macrophage Polarization by Fibrinogen-Like Protein 2. Front Immunol. 2018;9:87 pubmed publisher
  8. Leonardi I, Li X, Semon A, Li D, Doron I, Putzel G, et al. CX3CR1+ mononuclear phagocytes control immunity to intestinal fungi. Science. 2018;359:232-236 pubmed publisher
  9. Yang A, Inamine T, Hochrath K, Chen P, Wang L, Llorente C, et al. Intestinal fungi contribute to development of alcoholic liver disease. J Clin Invest. 2017;127:2829-2841 pubmed publisher
  10. Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
  11. Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
  12. Zhao Y, Chu X, Chen J, Wang Y, Gao S, Jiang Y, et al. Dectin-1-activated dendritic cells trigger potent antitumour immunity through the induction of Th9 cells. Nat Commun. 2016;7:12368 pubmed publisher
  13. Kanayama M, Inoue M, Danzaki K, Hammer G, He Y, Shinohara M. Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity. Nat Commun. 2015;6:5779 pubmed publisher
  14. Colton C. Heterogeneity of microglial activation in the innate immune response in the brain. J Neuroimmune Pharmacol. 2009;4:399-418 pubmed publisher