This is a Validated Antibody Database (VAD) review about mouse Cldn2, based on 56 published articles (read how Labome selects the articles), using Cldn2 antibody in all methods. It is aimed to help Labome visitors find the most suited Cldn2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cldn2 synonym: AL022813
Knockout validation
Invitrogen mouse monoclonal (12H12) |
| Invitrogen Cldn2 antibody (Thermofisher, 325600) was used in western blot knockout validation on human samples at 1:5000 (fig 2a) and in immunohistochemistry on human samples at 1:250 (fig 3a, 5d, s1). Commun Biol (2021) ncbi |
Invitrogen
mouse monoclonal (12H12) |
| Invitrogen Cldn2 antibody (Thermofisher, 325600) was used in western blot knockout validation on human samples at 1:5000 (fig 2a) and in immunohistochemistry on human samples at 1:250 (fig 3a, 5d, s1). Commun Biol (2021) ncbi |
mouse monoclonal (12H12) |
| Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry - paraffin section on human samples (fig 1d), in immunocytochemistry on human samples (fig 3l, 5b), in western blot on human samples (fig 4i), in immunoprecipitation on dogs samples (fig 6a), in immunocytochemistry on dogs samples (fig 3b), in immunohistochemistry - paraffin section on mouse samples (fig 2c) and in western blot on mouse samples (fig 2b). J Exp Clin Cancer Res (2021) ncbi |
mouse monoclonal (12H12) |
| In order to study the role of claudin-4 in tight junctions in MDCK cells, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunocytochemistry on dogs samples (fig 2) and in western blot on dogs samples (fig 2). PLoS ONE (2017) ncbi |
mouse monoclonal (12H12) |
| In order to measure tight junction protein expression in canine duodenum, lung, liver, and kidney, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry - paraffin section on dogs samples at 1:200 (fig 2b) and in western blot on dogs samples at 1:1000 (fig 1f). Mol Med Rep (2016) ncbi |
mouse monoclonal (12H12) |
| In order to establish methods to assess claudin-2 expression and localization in human colon biopsy samples, Invitrogen Cldn2 antibody (Invitrogen, 32?C5600) was used in immunohistochemistry - paraffin section on human samples (fig 4E), in immunocytochemistry on human samples (fig 2E) and in western blot on human samples (fig 1). PLoS ONE (2016) ncbi |
mouse monoclonal (12H12) |
| In order to explore the effects of mild chronic psychological stress on metabolic, inflammatory, and behavioral profiles in a mouse model of diet-induced obesity, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry on mouse samples at 7 ug/ml (tbl s5) and in western blot on mouse samples at 3 ug/ml (tbl s5). Brain Behav Immun (2017) ncbi |
mouse monoclonal (12H12) |
| In order to evaluate the effect of glucocorticoids in a mouse model of colitis, Invitrogen Cldn2 antibody (Thermo Fisher, 32-5600) was used in western blot on mouse samples at 1:1000 (fig 3a). Biochem Pharmacol (2016) ncbi |
domestic rabbit polyclonal |
| In order to study attenuation of oxazolone-induced colitis in mice through suppression of inflammataion and epithelial barrier disruption via modified pulsatilla decoction, Invitrogen Cldn2 antibody (Invitrogen, PA5-13335) was used in western blot on mouse samples at 1:1000 (fig 3). Mol Med Rep (2016) ncbi |
mouse monoclonal (12H12) |
| In order to study a nonhuman primate model of chronic gastrointestinal syndrome, Invitrogen Cldn2 antibody (Invitrogen, 325600) was used in western blot on rhesus macaque samples at 2 ug/ml (fig 9b). Radiat Res (2016) ncbi |
mouse monoclonal (12H12) |
| In order to characterize a reduced N and/or Ca diet by expression of tight junction proteins and cadherin 17 in the small intestine of young goats, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in western blot on domestic goat samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi |
mouse monoclonal (12H12) |
| In order to utilize a microwave antigen retrieval method by using two heating steps for enhanced immunostaining on tissue sections that are aldehyde-fixed and paraffin-embedded, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry - paraffin section on mouse samples (fig 1). Histochem Cell Biol (2016) ncbi |
mouse monoclonal (12H12) |
| In order to study OGR1-mediated, pH-dependent signaling pathways in intestinal epithelial cells, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in western blot on human samples . Am J Physiol Gastrointest Liver Physiol (2015) ncbi |
mouse monoclonal (12H12) |
| In order to investigate the interaction between ZO-1 and TOCA-1, Invitrogen Cldn2 antibody (Life Technologies, 32-5600) was used in immunocytochemistry on dogs samples (fig 3g) and in western blot on dogs samples (fig 3f). Mol Biol Cell (2015) ncbi |
mouse monoclonal (12H12) |
| In order to analyze breast cancer liver metastasis and how Lyn modulates Claudin-2 expression as a therapeutic target, Invitrogen Cldn2 antibody (Invitrogen, 325600) was used in western blot on human samples at 0.1 ug/ml (fig 1) and in western blot on mouse samples at 0.1 ug/ml (fig 1). Oncotarget (2015) ncbi |
mouse monoclonal (12H12) |
| In order to examine the mast cell proteinase and tight junction protein kinetics in a rat model of post-infectious gut dysfunction, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry on rat samples at 1:2000 (fig 9). J Crohns Colitis (2015) ncbi |
mouse monoclonal (12H12) |
| In order to characterize TALEN-mediated claudin-2 knockout in MDCK cells, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunocytochemistry on dogs samples and in western blot on dogs samples . PLoS ONE (2015) ncbi |
mouse monoclonal (12H12) |
| In order to investigate the effect of homoharringtonine on intestinal epithelial permeability and its mechanism, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in western blot on human samples . Eur J Pharm Biopharm (2015) ncbi |
mouse monoclonal (12H12) |
| In order to correlate expression of claudins with clinical outcomes of triple-negative breast cancer, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry on human samples at 1:100 (fig 1). PLoS ONE (2014) ncbi |
mouse monoclonal (12H12) |
| In order to perform longitudinal and cross-sectional studies of patients after restorative proctocolectomy and assess tight junction proteins and dendritic cell characteristics in the ileal pouch, Invitrogen Cldn2 antibody (Invitrogen, 12H12) was used in flow cytometry on human samples . Inflamm Bowel Dis (2014) ncbi |
mouse monoclonal (12H12) |
| In order to characterize the TALEN-mediated ZO-1 knockout in MDCK cells, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunocytochemistry on dogs samples and in western blot on dogs samples . PLoS ONE (2014) ncbi |
mouse monoclonal (12H12) |
| In order to assess the role of Toll-like receptor 2 in maintaining the integrity of the airway epithelial barrier, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunocytochemistry on human samples (fig 3). Tissue Barriers (2014) ncbi |
mouse monoclonal (12H12) |
| In order to investigate the localization of POF1B in desmosomes and its role in human intestinal and keratinocyte cell lines, Invitrogen Cldn2 antibody (Zymed Laboratories, 32-5600) was used in western blot on human samples . J Invest Dermatol (2015) ncbi |
mouse monoclonal (12H12) |
| In order to correlate claudin 1-5 and 7 expression with the survival, grade, and stage of patients with renal cell cancer, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples (fig 1b). Anticancer Res (2014) ncbi |
mouse monoclonal (12H12) |
| In order to assess the contribution of enteropathogenic Escherichia coli EspG-mediated microtubule disruption to tight junction perturbation, Invitrogen Cldn2 antibody (Life Technologies, 325600) was used in immunocytochemistry on human samples at 1:100 (fig 4). Cell Microbiol (2014) ncbi |
mouse monoclonal (12H12) |
| In order to study the role of TH9 cells in ulcerative colitis, Invitrogen Cldn2 antibody (Invitrogen, 12H12) was used in western blot on mouse samples (fig 6b). Nat Immunol (2014) ncbi |
mouse monoclonal (12H12) |
| In order to evaluate claudin 2 as a proximal tubular stress responsive paracellular water channel, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunocytochemistry on human samples at 1:300 and in western blot on human samples at 1:500. Toxicol Appl Pharmacol (2014) ncbi |
mouse monoclonal (12H12) |
| In order to study the involvement of TJP2 mautations in progressive cholestatic liver disease, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry on human samples and in western blot on human samples at 1:5000. Nat Genet (2014) ncbi |
mouse monoclonal (12H12) |
| In order to study the molecular processes involved in renal epithelial proximal-tubule monolayer maturation, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunocytochemistry on human samples (fig 3) and in western blot on human samples at 0.8 ug/ml (fig 3). Mol Cell Biol (2013) ncbi |
mouse monoclonal (12H12) |
| In order to study the effects of intestinal barrier proteins in necrotizing enterocolitis and test if Bifidobacterium infantis modulates tight junction proteins, Invitrogen Cldn2 antibody (Life Technologies, 32-5600) was used in immunohistochemistry - frozen section on mouse samples at 2.5 ug/ml (fig 4), in western blot on mouse samples and in immunohistochemistry - paraffin section on human samples . Am J Pathol (2013) ncbi |
mouse monoclonal (12H12) |
| In order to examine innate responses to Entamoeba histolytica in wild-type and MUC-2-deficient mice, Invitrogen Cldn2 antibody (Invitrogen, 325600) was used in immunohistochemistry - frozen section on mouse samples (fig 3). Am J Pathol (2013) ncbi |
mouse monoclonal (12H12) |
| In order to examine tight junction and adherens junction proteins in normal human lung, IPF, cryptogenic organizing pneumonia, and asbestosis, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1). Hum Pathol (2013) ncbi |
mouse monoclonal (12H12) |
| In order to identify genetic variants associated with pancreatitis, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 2) and in western blot on human samples (fig 2). Nat Genet (2012) ncbi |
mouse monoclonal (12H12) |
| In order to report that mice lacking EpCAM have intestinal barrier defects and die shortly after birth as a result of intestinal erosion, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunohistochemistry - frozen section on mouse samples at 1:250 (fig 4), in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples at 1:500 (fig 2). Dev Biol (2012) ncbi |
mouse monoclonal (12H12) |
| In order to determine the effects of KBrO(3) using human and rat proximal tubular cell lines, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunocytochemistry on human samples at 1:300 (fig 4) and in western blot on human samples at 1:600 (fig 6). Arch Toxicol (2012) ncbi |
mouse monoclonal (12H12) |
| In order to assess the role of tight junctions and gap proteins in cervical remodeling, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunohistochemistry - frozen section on human samples at 1:100 and in western blot on human samples at 1:1000 (fig 2). J Transl Med (2012) ncbi |
mouse monoclonal (12H12) |
| In order to examine the effect of treating epithelial cells with an inhibitor of the lipid kinase PIKfyve, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunocytochemistry on dogs samples (fig 1) and in western blot on dogs samples (fig 2, 4). PLoS ONE (2012) ncbi |
mouse monoclonal (12H12) |
| In order to analyze the role of paracingulin domains, Invitrogen Cldn2 antibody (Invitrogen, 32-5600) was used in western blot on dogs samples (fig 1). J Biol Chem (2012) ncbi |
mouse monoclonal (12H12) |
| In order to identify Cldn1, Cldn2 and Cldn11 as genes that discriminate between diverse types of M2, Invitrogen Cldn2 antibody (Invitrogen, 12H12) was used in flow cytometry on mouse samples (fig 3) and in western blot on mouse samples (fig s1). Scand J Immunol (2012) ncbi |
mouse monoclonal (12H12) |
| In order to demonstrate that ESCRT proteins are required to maintain polarity in mammalian epithelial cells, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunocytochemistry on dogs samples at 1:25 (fig s7). Mol Biol Cell (2011) ncbi |
mouse monoclonal (12H12) |
| In order to determine claudin expression profiles in Epstein-Barr virus-associated non-keratinizing nasopharyngeal carcinoma, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples (fig 3). Oncol Rep (2010) ncbi |
mouse monoclonal (12H12) |
| In order to examine the consequences of HNF-4alpha loss in the adult intestinal epithelium, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in western blot on mouse samples at 1:100 (fig 10). Mol Cell Biol (2009) ncbi |
mouse monoclonal (12H12) |
| In order to study the role of claudin-2 in bile canalicular formation, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunocytochemistry on rat samples at 1:100 (tbl 3). Histochem Cell Biol (2009) ncbi |
mouse monoclonal (12H12) |
| In order to determine the expression of several different claudins in rectal well-differentiated endocrine neoplasms, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 2). Oncol Rep (2009) ncbi |
mouse monoclonal (12H12) |
| In order to report the expression of various claudins in patients with sarcoidosis, usual interstitial pneumonia, and normal human lung, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples (fig 1b). J Histochem Cytochem (2009) ncbi |
mouse monoclonal (12H12) |
| In order to elucidate how the keratin intermediate filament network is involved in the establishment and maintenance of cell polarity, Invitrogen Cldn2 antibody (Invitrogen, 12H12) was used in western blot on human samples (fig 1). J Cell Biol (2008) ncbi |
mouse monoclonal (12H12) |
| In order to test if altered expression of intestinal epithelial tight junction proteins contributes to neoplastic progression, Invitrogen Cldn2 antibody (Invitrogen, 12H12) was used in immunohistochemistry - paraffin section on human samples (fig 1). Lab Invest (2008) ncbi |
mouse monoclonal (12H12) |
| In order to study the role of CLDN2 expression in breast cancer development and progression, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3). Histopathology (2008) ncbi |
mouse monoclonal (12H12) |
| In order to study the effects of cingulin overexpression, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in western blot on dogs samples (fig 3). Mol Membr Biol (2008) ncbi |
mouse monoclonal (12H12) |
| In order to determine whether transforming growth factor-beta down-regulates claudin-1 expression to induce epithelial to mesenchymal transition, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunocytochemistry on rat samples at 1:100 (fig 2B). Liver Int (2008) ncbi |
mouse monoclonal (12H12) |
| In order to correlate glutamine synthetase immunostaining and beta-catenin mutations with clinicopathological features in hepatocellular carcinomas, Invitrogen Cldn2 antibody (Zymed, clone 12H12) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 4). J Pathol (2007) ncbi |
mouse monoclonal (12H12) |
| In order to investigate the function of cingulin in tight junctions, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunocytochemistry on dogs samples (fig 4) and in western blot on dogs samples (fig 1). Mol Biol Cell (2006) ncbi |
mouse monoclonal (12H12) |
| In order to observe the expression of claudins in mesothelioma and metastatic pleural adenocarcinoma, Invitrogen Cldn2 antibody (Zymed, 12H12) was used in immunohistochemistry - paraffin section on human samples at 1:50. J Clin Pathol (2006) ncbi |
mouse monoclonal (12H12) |
| In order to characterize cell lines that mimic the characteristics of the proximal tubule, Invitrogen Cldn2 antibody (Zymed, 32-5600) was used in immunocytochemistry on rat samples , in immunocytochemistry on American opossums samples , in immunocytochemistry on pigs |
Abcam
domestic rabbit polyclonal |
| Abcam Cldn2 antibody (Abcam, ab53032) was used in immunohistochemistry on mouse samples at 1:1000 (fig s3a). Redox Biol (2021) ncbi |
domestic rabbit polyclonal |
| Abcam Cldn2 antibody (Abcam, ab53032) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s2c), in western blot on mouse samples at 1:500 (fig s2a), in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2c) and in western blot on human samples at 1:500 (fig 2a). MBio (2020) ncbi |
domestic rabbit polyclonal |
| Abcam Cldn2 antibody (Abcam, ab53032) was used in immunohistochemistry on mouse samples at 1:200 (fig 2d). Cell Mol Gastroenterol Hepatol (2019) ncbi |
Articles Reviewed
- de Sousa Rodrigues M, Bekhbat M, Houser M, Chang J, Walker D, Jones D, et al. Chronic psychological stress and high-fat high-fructose diet disrupt metabolic and inflammatory gene networks in the brain, liver, and gut and promote behavioral deficits in mice. Brain Behav Immun. 2017;59:158-172 pubmed publisher
- Tabariès S, Annis M, Hsu B, Tam C, Savage P, Park M, et al. Lyn modulates Claudin-2 expression and is a therapeutic target for breast cancer liver metastasis. Oncotarget. 2015;6:9476-87 pubmed
- Virman J, Soini Y, Kujala P, Luukkaala T, Salminen T, Sunela K, et al. Claudins as prognostic factors for renal cell cancer. Anticancer Res. 2014;34:4181-7 pubmed
- Van den Bossche J, Laoui D, Morias Y, Movahedi K, Raes G, De Baetselier P, et al. Claudin-1, claudin-2 and claudin-11 genes differentially associate with distinct types of anti-inflammatory macrophages in vitro and with parasite- and tumour-elicited macrophages in vivo. Scand J Immunol. 2012;75:588-98 pubmed publisher
- Kojima F, Ishida M, Takikita Suzuki M, Hotta M, Katsura K, Nagata A, et al. Claudin expression profiles in Epstein-Barr virus-associated nasopharyngeal carcinoma. Oncol Rep. 2010;23:927-31 pubmed
- Ishida M, Kushima R, Okabe H. Claudin expression in rectal well-differentiated endocrine neoplasms (carcinoid tumors). Oncol Rep. 2009;21:113-7 pubmed
- Paschoud S, Citi S. Inducible overexpression of cingulin in stably transfected MDCK cells does not affect tight junction organization and gene expression. Mol Membr Biol. 2008;25:1-13 pubmed
- Kojima T, Takano K, Yamamoto T, Murata M, Son S, Imamura M, et al. Transforming growth factor-beta induces epithelial to mesenchymal transition by down-regulation of claudin-1 expression and the fence function in adult rat hepatocytes. Liver Int. 2008;28:534-45 pubmed
- Audard V, Grimber G, Elie C, Radenen B, Audebourg A, Letourneur F, et al. Cholestasis is a marker for hepatocellular carcinomas displaying beta-catenin mutations. J Pathol. 2007;212:345-52 pubmed
- Guillemot L, Citi S. Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA. Mol Biol Cell. 2006;17:3569-77 pubmed
- Soini Y, Kinnula V, Kahlos K, Paakko P. Claudins in differential diagnosis between mesothelioma and metastatic adenocarcinoma of the pleura. J Clin Pathol. 2006;59:250-4 pubmed
- Prozialeck W, Edwards J, Lamar P, Smith C. Epithelial barrier characteristics and expression of cell adhesion molecules in proximal tubule-derived cell lines commonly used for in vitro toxicity studies. Toxicol In Vitro. 2006;20:942-53 pubmed
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