This is a Validated Antibody Database (VAD) review about mouse Cdc27, based on 21 published articles (read how Labome selects the articles), using Cdc27 antibody in all methods. It is aimed to help Labome visitors find the most suited Cdc27 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cdc27 synonym: AI452358; APC3; BC023187; cell division cycle protein 27 homolog; cell division cycle 27 homolog

Santa Cruz Biotechnology
mouse monoclonal (AF3.1)
  • western blot; human; fig 3
In order to investigate activation of APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth by TGF-beta, Santa Cruz Biotechnology Cdc27 antibody (Santa Cruz, sc-9972) was used in western blot on human samples (fig 3). Cell Cycle (2016) ncbi
mouse monoclonal (AF3.1)
  • immunohistochemistry - paraffin section; human; 1:50; fig 1
  • western blot; human; 1:200; fig 1
Santa Cruz Biotechnology Cdc27 antibody (Santa Cruz, sc-9972) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1) and in western blot on human samples at 1:200 (fig 1). Cell Death Dis (2016) ncbi
mouse monoclonal (AF3.1)
  • western blot; human; 1:1000
In order to study CDC26 phosphorylation and its effect on the tetratricopeptide repeat subcomplex of APC/C, Santa Cruz Biotechnology Cdc27 antibody (Santa Cruz Biotechnology, sc-9972) was used in western blot on human samples at 1:1000. PLoS ONE (2015) ncbi
mouse monoclonal (C-4)
  • western blot; human
Santa Cruz Biotechnology Cdc27 antibody (Santa Cruz, sc-13154) was used in western blot on human samples . Cell Death Dis (2014) ncbi
mouse monoclonal (AF3.1)
  • western blot; human; fig 6c
Santa Cruz Biotechnology Cdc27 antibody (Santa, AF3.1) was used in western blot on human samples (fig 6c). Cell (2014) ncbi
mouse monoclonal (C-4)
  • western blot; human
Santa Cruz Biotechnology Cdc27 antibody (Santa Cruz, sc-13154) was used in western blot on human samples . Mol Cell Biol (2013) ncbi
BD Biosciences
mouse monoclonal (35/CDC27)
  • western blot; human; loading ...; fig 1f
BD Biosciences Cdc27 antibody (BD, 610454) was used in western blot on human samples (fig 1f). Nat Cell Biol (2017) ncbi
mouse monoclonal (35/CDC27)
  • immunoprecipitation; mouse; loading ...; fig 3b
  • western blot; mouse; loading ...; fig 3b
In order to report that the anaphase-promoting complex/cyclosome-mediated degradation of Rock2 maintains the dendritic network, memory formation, and neuronal survival, BD Biosciences Cdc27 antibody (BD Pharmingen, 35/CDC27) was used in immunoprecipitation on mouse samples (fig 3b) and in western blot on mouse samples (fig 3b). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (35/CDC27)
  • western blot; mouse; fig s3
BD Biosciences Cdc27 antibody (BD, 610455) was used in western blot on mouse samples (fig s3). Nat Cell Biol (2016) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; 1:1000; fig 2
BD Biosciences Cdc27 antibody (BD Biosciences, 610454) was used in western blot on human samples at 1:1000 (fig 2). Nat Commun (2016) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; 1:500; fig 1
BD Biosciences Cdc27 antibody (BD Biosciences, 610454) was used in western blot on human samples at 1:500 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (35/CDC27)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Cdc27 antibody (BD, 35) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; loading ...; fig 4c
In order to present the role of nuclear pore protein nup88 on anaphase-promoting complex, BD Biosciences Cdc27 antibody (BD Transduction Laboratories, 610455) was used in western blot on human samples (fig 4c). J Clin Invest (2016) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; fig 2
BD Biosciences Cdc27 antibody (BD Transduction, 610455) was used in western blot on human samples (fig 2). Sci Rep (2015) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; 1:1000; fig 1
BD Biosciences Cdc27 antibody (BD Transduction laboratories, 610454) was used in western blot on human samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, BD Biosciences Cdc27 antibody (BD Transduction, 610455) was used in western blot on human samples . Biol Open (2015) ncbi
mouse monoclonal (35/CDC27)
  • immunoprecipitation; human; 1:500; fig s3
BD Biosciences Cdc27 antibody (BD Transduction Laboratories, 610455) was used in immunoprecipitation on human samples at 1:500 (fig s3). Nature (2015) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; 1:500
BD Biosciences Cdc27 antibody (BD, 610454) was used in western blot on human samples at 1:500. EMBO Rep (2014) ncbi
mouse monoclonal (35/CDC27)
  • western blot; human; 1:1000
In order to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism, BD Biosciences Cdc27 antibody (BD Transduction Laboratories, 610454) was used in western blot on human samples at 1:1000. Virology (2013) ncbi
Sigma-Aldrich
mouse monoclonal (AF3.1)
  • western blot; rat; fig 3b
Sigma-Aldrich Cdc27 antibody (Sigma-Aldrich, C7104) was used in western blot on rat samples (fig 3b). Sci Rep (2016) ncbi
mouse monoclonal (AF3.1)
  • western blot; human; 1:1000; fig 1
In order to report how Cep68 regulates centriole disengagement, Sigma-Aldrich Cdc27 antibody (Sigma, AF3.1) was used in western blot on human samples at 1:1000 (fig 1). Nat Cell Biol (2015) ncbi
Articles Reviewed
  1. Hein J, Hertz E, Garvanska D, Kruse T, Nilsson J. Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis. Nat Cell Biol. 2017;19:1433-1440 pubmed publisher
  2. Bobo Jiménez V, Delgado Esteban M, Angibaud J, Sánchez Morán I, de la Fuente A, Yajeya J, et al. APC/CCdh1-Rock2 pathway controls dendritic integrity and memory. Proc Natl Acad Sci U S A. 2017;114:4513-4518 pubmed publisher
  3. Bott L, Salomons F, Maric D, Liu Y, Merry D, Fischbeck K, et al. The polyglutamine-expanded androgen receptor responsible for spinal and bulbar muscular atrophy inhibits the APC/C(Cdh1) ubiquitin ligase complex. Sci Rep. 2016;6:27703 pubmed publisher
  4. van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;18:814-21 pubmed publisher
  5. Iimori M, Watanabe S, Kiyonari S, Matsuoka K, Sakasai R, Saeki H, et al. Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability. Nat Commun. 2016;7:11117 pubmed publisher
  6. Pavlides S, Lecanda J, Daubriac J, Pandya U, Gama P, Blank S, et al. TGF-β activates APC through Cdh1 binding for Cks1 and Skp2 proteasomal destruction stabilizing p27kip1 for normal endometrial growth. Cell Cycle. 2016;15:931-47 pubmed publisher
  7. Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher
  8. Qiu L, Wu J, Pan C, Tan X, Lin J, Liu R, et al. Downregulation of CDC27 inhibits the proliferation of colorectal cancer cells via the accumulation of p21Cip1/Waf1. Cell Death Dis. 2016;7:e2074 pubmed publisher
  9. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  10. Naylor R, Jeganathan K, Cao X, van Deursen J. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest. 2016;126:543-59 pubmed publisher
  11. Voets E, Marsman J, Demmers J, Beijersbergen R, Wolthuis R. The lethal response to Cdk1 inhibition depends on sister chromatid alignment errors generated by KIF4 and isoform 1 of PRC1. Sci Rep. 2015;5:14798 pubmed publisher
  12. de Lange J, Faramarz A, Oostra A, de Menezes R, van der Meulen I, Rooimans M, et al. Defective sister chromatid cohesion is synthetically lethal with impaired APC/C function. Nat Commun. 2015;6:8399 pubmed publisher
  13. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  14. Masuda K, Chiyoda T, Sugiyama N, Segura Cabrera A, Kabe Y, Ueki A, et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS ONE. 2015;10:e0118662 pubmed publisher
  15. Pagan J, Marzio A, Jones M, Saraf A, Jallepalli P, Florens L, et al. Degradation of Cep68 and PCNT cleavage mediate Cep215 removal from the PCM to allow centriole separation, disengagement and licensing. Nat Cell Biol. 2015;17:31-43 pubmed publisher
  16. Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  17. Ohoka N, Nagai K, Hattori T, Okuhira K, Shibata N, Cho N, et al. Cancer cell death induced by novel small molecules degrading the TACC3 protein via the ubiquitin-proteasome pathway. Cell Death Dis. 2014;5:e1513 pubmed publisher
  18. Meyer H, Rape M. Enhanced protein degradation by branched ubiquitin chains. Cell. 2014;157:910-21 pubmed publisher
  19. Hein J, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. EMBO Rep. 2014;15:264-72 pubmed publisher
  20. Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  21. Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher