This is a Validated Antibody Database (VAD) review about mouse Cd74, based on 20 published articles (read how Labome selects the articles), using Cd74 antibody in all methods. It is aimed to help Labome visitors find the most suited Cd74 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cd74 synonym: CLIP; DHLAG; HLADG; Ia-GAMMA; Ii

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (LN-2)
  • western blot knockout validation; mouse; loading ...; fig 4d
  • flow cytometry; human; loading ...; fig 1g
  • immunohistochemistry; human; loading ...; fig 1b
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, LN-2) was used in western blot knockout validation on mouse samples (fig 4d), in flow cytometry on human samples (fig 1g) and in immunohistochemistry on human samples (fig 1b). Cell Mol Gastroenterol Hepatol (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • flow cytometry; human; loading ...; fig 1g
  • immunohistochemistry; human; loading ...; fig 1b
  • western blot knockout validation; mouse; loading ...; fig 4d
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, LN-2) was used in flow cytometry on human samples (fig 1g), in immunohistochemistry on human samples (fig 1b) and in western blot knockout validation on mouse samples (fig 4d). Cell Mol Gastroenterol Hepatol (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (LN-2)
  • western blot knockout validation; mouse; loading ...; fig 4d
  • flow cytometry; human; loading ...; fig 1g
  • immunohistochemistry; human; loading ...; fig 1b
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, LN-2) was used in western blot knockout validation on mouse samples (fig 4d), in flow cytometry on human samples (fig 1g) and in immunohistochemistry on human samples (fig 1b). Cell Mol Gastroenterol Hepatol (2020) ncbi
mouse monoclonal
  • flow cytometry; human; loading ...; fig 1g
  • immunohistochemistry; human; loading ...; fig 1b
  • western blot knockout validation; mouse; loading ...; fig 4d
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, LN-2) was used in flow cytometry on human samples (fig 1g), in immunohistochemistry on human samples (fig 1b) and in western blot knockout validation on mouse samples (fig 4d). Cell Mol Gastroenterol Hepatol (2020) ncbi
mouse monoclonal
  • western blot; human; fig 2b
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz Biotechnology, LN-2) was used in western blot on human samples (fig 2b). J Immunol (2019) ncbi
mouse monoclonal (LN-2)
  • western blot; human; fig 2b
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz Biotechnology, LN-2) was used in western blot on human samples (fig 2b). J Immunol (2019) ncbi
mouse monoclonal (PIN.1)
  • western blot; human; 1:500; loading ...; fig 2b
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Santa Cruz Biotechnology Cd74 antibody (Santa Cruz Biotechnology, sc-47742) was used in western blot on human samples at 1:500 (fig 2b). Nat Commun (2017) ncbi
mouse monoclonal (LN-2)
  • immunocytochemistry; human; 1:200; loading ...; fig 3h
  • western blot; human; 1:1000; loading ...; fig 2b
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Santa Cruz Biotechnology Cd74 antibody (Santa Cruz Biotechnology, sc-6262) was used in immunocytochemistry on human samples at 1:200 (fig 3h) and in western blot on human samples at 1:1000 (fig 2b). Nat Commun (2017) ncbi
mouse monoclonal (CerCLIP.1)
  • immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; 1:3000; loading ...; fig 2a
In order to study the antigen presentation by HLA-DP84Gly through the class I antigen processing pathway, Santa Cruz Biotechnology Cd74 antibody (Santa Cruz Biotechnology, sc-12725) was used in immunoprecipitation on human samples (fig 2c) and in western blot on human samples at 1:3000 (fig 2a). Nat Commun (2017) ncbi
mouse monoclonal (LN-2)
  • blocking or activating experiments; human
  • flow cytometry; human; fig 2b
In order to explore how the CD74/CD44 MIF receptor contributes to rheumatoid arthritis, Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, LN2) was used in blocking or activating experiments on human samples and in flow cytometry on human samples (fig 2b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal
  • flow cytometry; human; fig 2b
In order to explore how the CD74/CD44 MIF receptor contributes to rheumatoid arthritis, Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, LN2) was used in flow cytometry on human samples (fig 2b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (CerCLIP.1)
  • flow cytometry; human; loading ...; fig 1a
In order to study Type 1 diabetes, HLA-DQ2 and DQ8 molecules, and HLA-DM effect on invariant chain-derived CLIP peptides, Santa Cruz Biotechnology Cd74 antibody (SantaCruz, Cer.CLIP1) was used in flow cytometry on human samples (fig 1a). Eur J Immunol (2016) ncbi
mouse monoclonal (LN-2)
  • ELISA; human; fig 2
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, sc-6262) was used in ELISA on human samples (fig 2). Antioxid Redox Signal (2015) ncbi
mouse monoclonal (D-6)
  • flow cytometry; mouse
Santa Cruz Biotechnology Cd74 antibody (Santa Cruz, sc-137217) was used in flow cytometry on mouse samples . Monoclon Antib Immunodiagn Immunother (2014) ncbi
BioLegend
rat monoclonal (In1/CD74)
  • flow cytometry; mouse; fig 4i
BioLegend Cd74 antibody (Biolegend, 151002) was used in flow cytometry on mouse samples (fig 4i). Cancer Cell (2021) ncbi
rat monoclonal (In1/CD74)
  • immunohistochemistry - frozen section; mouse; 2,000 ug/ml; loading ...; fig 2i
BioLegend Cd74 antibody (BioLegend, In1/CD74) was used in immunohistochemistry - frozen section on mouse samples at 2,000 ug/ml (fig 2i). Science (2019) ncbi
Abcam
mouse monoclonal (LN2)
  • western blot; human; 1:1000; loading ...; fig 11e
Abcam Cd74 antibody (Abcam, ab9514) was used in western blot on human samples at 1:1000 (fig 11e). J Biol Chem (2018) ncbi
Miltenyi Biotec
mouse monoclonal (AC122)
  • flow cytometry; human; loading ...; fig 4c
Miltenyi Biotec Cd74 antibody (Miltenyi Biotec, AC122) was used in flow cytometry on human samples (fig 4c). Cell Death Dis (2019) ncbi
BD Biosciences
rat monoclonal (In-1)
  • flow cytometry; mouse; loading ...
BD Biosciences Cd74 antibody (BD, In1) was used in flow cytometry on mouse samples . NPJ Aging (2022) ncbi
rat monoclonal (In-1)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5s1a
BD Biosciences Cd74 antibody (BD, 555318) was used in immunohistochemistry on mouse samples at 1:100 (fig 5s1a). elife (2019) ncbi
rat monoclonal (In-1)
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 3b
In order to propose a tumor-suppressive activity for macrophage migration inhibitory factor in murine skin, BD Biosciences Cd74 antibody (Becton Dickinson, In-1) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 3b). FASEB J (2017) ncbi
rat monoclonal (In-1)
  • western blot; mouse; 1:1000; fig 4
BD Biosciences Cd74 antibody (BD Biosciences, 555317) was used in western blot on mouse samples at 1:1000 (fig 4). Mol Cancer (2016) ncbi
rat monoclonal (In-1)
  • western blot; mouse
In order to study molecular mechanisms used by B cells to control the source of peptides loaded onto class II molecules, BD Biosciences Cd74 antibody (BD Biosciences, 555317) was used in western blot on mouse samples . J Biol Chem (2015) ncbi
rat monoclonal (In-1)
  • flow cytometry; mouse; 1:100
BD Biosciences Cd74 antibody (BD Biosciences, In-1) was used in flow cytometry on mouse samples at 1:100. Nat Commun (2015) ncbi
rat monoclonal (In-1)
  • immunocytochemistry; human; loading ...; fig 1d
  • western blot; human; loading ...; fig 5e
BD Biosciences Cd74 antibody (BD Biosciences, In-1) was used in immunocytochemistry on human samples (fig 1d) and in western blot on human samples (fig 5e). Traffic (2015) ncbi
rat monoclonal (In-1)
  • western blot; mouse; 1:10,000; fig 2
BD Biosciences Cd74 antibody (BD, In-1) was used in western blot on mouse samples at 1:10,000 (fig 2). J Cell Biol (2015) ncbi
rat monoclonal (In-1)
  • immunocytochemistry; mouse; fig 5d
  • western blot; mouse; fig 2d
In order to test if pharmacological modulation of SPPL2a can deplete B cells, BD Biosciences Cd74 antibody (BD, In-1) was used in immunocytochemistry on mouse samples (fig 5d) and in western blot on mouse samples (fig 2d). Mol Cell Biol (2014) ncbi
Articles Reviewed
  1. Davis C, Zhang W, Bah T, Roese N, Allen E, Leung P, et al. Age-dependent cognitive impairment, hydrocephalus and leukocyte infiltration in transgenic mice with endothelial expression of human EPHX2. NPJ Aging. 2022;8:9 pubmed publisher
  2. Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
  3. Farr L, Ghosh S, Jiang N, Watanabe K, Parlak M, Bucala R, et al. CD74 Signaling Links Inflammation to Intestinal Epithelial Cell Regeneration and Promotes Mucosal Healing. Cell Mol Gastroenterol Hepatol. 2020;10:101-112 pubmed publisher
  4. Dumont A, de Rosny C, Kieu T, Perrey S, Berger H, Fluckiger A, et al. Docosahexaenoic acid inhibits both NLRP3 inflammasome assembly and JNK-mediated mature IL-1β secretion in 5-fluorouracil-treated MDSC: implication in cancer treatment. Cell Death Dis. 2019;10:485 pubmed publisher
  5. Crivello P, Ahci M, Maaßen F, Wossidlo N, Arrieta Bolaños E, Heinold A, et al. Multiple Knockout of Classical HLA Class II β-Chains by CRISPR/Cas9 Genome Editing Driven by a Single Guide RNA. J Immunol. 2019;202:1895-1903 pubmed publisher
  6. Jordão M, Sankowski R, Brendecke S, Sagar -, Locatelli G, Tai Y, et al. Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation. Science. 2019;363: pubmed publisher
  7. Ma W, Silverman S, Zhao L, Villasmil R, Campos M, Amaral J, et al. Absence of TGFβ signaling in retinal microglia induces retinal degeneration and exacerbates choroidal neovascularization. elife. 2019;8: pubmed publisher
  8. De R, Sarkar S, Mazumder S, Debsharma S, Siddiqui A, Saha S, et al. Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-κB signaling. J Biol Chem. 2018;293:19740-19760 pubmed publisher
  9. Yamashita Y, Anczurowski M, Nakatsugawa M, Tanaka M, Kagoya Y, Sinha A, et al. HLA-DP84Gly constitutively presents endogenous peptides generated by the class I antigen processing pathway. Nat Commun. 2017;8:15244 pubmed publisher
  10. Yoo S, Leng L, Kim B, Du X, Tilstam P, Kim K, et al. MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis. Proc Natl Acad Sci U S A. 2016;113:E7917-E7926 pubmed
  11. Brocks T, Fedorchenko O, Schliermann N, Stein A, Moll U, Seegobin S, et al. Macrophage migration inhibitory factor protects from nonmelanoma epidermal tumors by regulating the number of antigen-presenting cells in skin. FASEB J. 2017;31:526-543 pubmed publisher
  12. Wilkinson R, Young A, Burden R, Williams R, Scott C. A bioavailable cathepsin S nitrile inhibitor abrogates tumor development. Mol Cancer. 2016;15:29 pubmed publisher
  13. Zhou Z, Reyes Vargas E, Escobar H, Rudd B, Rockwood A, Delgado J, et al. Type 1 diabetes associated HLA-DQ2 and DQ8 molecules are relatively resistant to HLA-DM mediated release of invariant chain-derived CLIP peptides. Eur J Immunol. 2016;46:834-45 pubmed publisher
  14. Barroso M, Tucker H, Drake L, Nichol K, Drake J. Antigen-B Cell Receptor Complexes Associate with Intracellular major histocompatibility complex (MHC) Class II Molecules. J Biol Chem. 2015;290:27101-12 pubmed publisher
  15. Stoppe C, Rex S, Goetzenich A, Kraemer S, Emontzpohl C, Soppert J, et al. Interaction of MIF Family Proteins in Myocardial Ischemia/Reperfusion Damage and Their Influence on Clinical Outcome of Cardiac Surgery Patients. Antioxid Redox Signal. 2015;23:865-79 pubmed publisher
  16. Rouhani S, Eccles J, Riccardi P, Peske J, Tewalt E, Cohen J, et al. Roles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction. Nat Commun. 2015;6:6771 pubmed publisher
  17. Mentrup T, Häsler R, Fluhrer R, Saftig P, Schröder B. A Cell-Based Assay Reveals Nuclear Translocation of Intracellular Domains Released by SPPL Proteases. Traffic. 2015;16:871-92 pubmed publisher
  18. Otomo T, Schweizer M, Kollmann K, Schumacher V, Muschol N, Tolosa E, et al. Mannose 6 phosphorylation of lysosomal enzymes controls B cell functions. J Cell Biol. 2015;208:171-80 pubmed publisher
  19. Genève L, Gauthier C, Thibodeau J. The D-6 mouse monoclonal antibody recognizes the CD74 cytoplasmic tail. Monoclon Antib Immunodiagn Immunother. 2014;33:221-7 pubmed publisher
  20. Schneppenheim J, Hüttl S, Mentrup T, Lüllmann Rauch R, Rothaug M, Engelke M, et al. The intramembrane proteases signal Peptide peptidase-like 2a and 2b have distinct functions in vivo. Mol Cell Biol. 2014;34:1398-411 pubmed publisher