This is a Validated Antibody Database (VAD) review about mouse Ccl12, based on 18 published articles (read how Labome selects the articles), using Ccl12 antibody in all methods. It is aimed to help Labome visitors find the most suited Ccl12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ccl12 synonym: MCP-5; Scya12

Novus Biologicals
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5e
Novus Biologicals Ccl12 antibody (Novus, NBP2-22115) was used in immunohistochemistry - paraffin section on mouse samples (fig 5e). Cell Death Dis (2022) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig e10g
Novus Biologicals Ccl12 antibody (Novus, NBP2- 2211555) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig e10g). Nature (2019) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 2d
  • immunohistochemistry - paraffin section; human; 1:4000; loading ...; fig 3f
Novus Biologicals Ccl12 antibody (Novus Biologicals, NBP-22115) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 2d) and in immunohistochemistry - paraffin section on human samples at 1:4000 (fig 3f). Nat Commun (2019) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry; mouse; loading ...; fig 3b
Novus Biologicals Ccl12 antibody (Novus Biologicals, 2D8) was used in immunohistochemistry on mouse samples (fig 3b). Diabetes (2017) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; fig 4
Novus Biologicals Ccl12 antibody (Novus, NBP2-22115S) was used in western blot on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:100
In order to investigate the relationship between macrophages and T-box transcription factor Brachyury in tumor progression and metastasis, Novus Biologicals Ccl12 antibody (Novus Biologicals, NBP2-22115) was used in immunohistochemistry - paraffin section on human samples at 1:100. Tumour Biol (2015) ncbi
Santa Cruz Biotechnology
rat monoclonal (ECE.2)
  • western blot; mouse; 1:200; loading ...; fig 5b
Santa Cruz Biotechnology Ccl12 antibody (Santa Cruz, sc-52701) was used in western blot on mouse samples at 1:200 (fig 5b). J Neuroinflammation (2021) ncbi
rat monoclonal (ECE.2)
  • western blot; mouse; 1:1000; loading ...; fig 2g
Santa Cruz Biotechnology Ccl12 antibody (Santa Cruz, sc-52701) was used in western blot on mouse samples at 1:1000 (fig 2g). Cell Death Dis (2021) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 6q
  • western blot; mouse; loading ...; fig 6m
Invitrogen Ccl12 antibody (ThermoFisher Scientific, PA5-34505) was used in immunohistochemistry on mouse samples (fig 6q) and in western blot on mouse samples (fig 6m). iScience (2021) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; 1:400; loading ...; fig 6c
Invitrogen Ccl12 antibody (Invitrogen, MA5-17040) was used in western blot on mouse samples at 1:400 (fig 6c). Nat Commun (2020) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; fig 3
In order to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis, Invitrogen Ccl12 antibody (Thermo Fisher Scientific, MA5-17040) was used in western blot on mouse samples (fig 3). J Orthop Res (2017) ncbi
hamsters monoclonal (2H5)
  • ELISA; human; fig 1b
  • ELISA; mouse; fig 2b
In order to optimize innate defense regulator derivatives with enhanced chemokine induction, Invitrogen Ccl12 antibody (eBioscience, 2H5) was used in ELISA on human samples (fig 1b) and in ELISA on mouse samples (fig 2b). J Immunol (2010) ncbi
BioLegend
hamsters monoclonal (2H5)
  • blocking or activating experiments; mouse; ; fig 3g
  • flow cytometry; mouse; loading ...; fig 4a
BioLegend Ccl12 antibody (BioLegend, 2H5) was used in blocking or activating experiments on mouse samples at (fig 3g) and in flow cytometry on mouse samples (fig 4a). Sci Adv (2020) ncbi
hamsters monoclonal (2H5)
  • immunohistochemistry - paraffin section; mouse
BioLegend Ccl12 antibody (Biolegend, 505902) was used in immunohistochemistry - paraffin section on mouse samples . Cardiovasc Res (2015) ncbi
hamsters monoclonal (2H5)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend Ccl12 antibody (Biolegend, 505902) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...
Cell Signaling Technology Ccl12 antibody (Cell Signalling Technology, 2029) was used in western blot on mouse samples at 1:1000. Brain Pathol (2021) ncbi
BD Biosciences
hamsters monoclonal (2H5)
  • blocking or activating experiments; mouse; fig 1A
BD Biosciences Ccl12 antibody (BD, 554440) was used in blocking or activating experiments on mouse samples (fig 1A). BMC Cancer (2017) ncbi
hamsters monoclonal (2H5)
  • flow cytometry; mouse; fig 3
In order to identify the source of obesity-induced MCP-1 and identify molecular regulators mediating MCP-1 production, BD Biosciences Ccl12 antibody (BD Bioscience, 2H5) was used in flow cytometry on mouse samples (fig 3). Mol Metab (2015) ncbi
Articles Reviewed
  1. Jin Y, Liu Y, Xu L, Xu J, Xiong Y, Peng Y, et al. Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosis. Cell Death Dis. 2022;13:512 pubmed publisher
  2. Fu H, Gui Y, Liu S, Wang Y, Bastacky S, Qiao Y, et al. The hepatocyte growth factor/c-met pathway is a key determinant of the fibrotic kidney local microenvironment. iScience. 2021;24:103112 pubmed publisher
  3. Takahashi K, Nakamura S, Otsu W, Shimazawa M, Hara H. Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation. J Neuroinflammation. 2021;18:164 pubmed publisher
  4. Chen C, Wang D, Yu Y, Zhao T, Min N, Wu Y, et al. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI. Cell Death Dis. 2021;12:65 pubmed publisher
  5. Song M, YEKU O, Rafiq S, Purdon T, Dong X, Zhu L, et al. Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages. Nat Commun. 2020;11:6298 pubmed publisher
  6. Kamali S, Rajendran R, Stadelmann C, Karnati S, Rajendran V, Giraldo Velasquez M, et al. Oligodendrocyte-specific deletion of FGFR2 ameliorates MOG35-55 -induced EAE through ERK and Akt signalling. Brain Pathol. 2021;31:297-311 pubmed publisher
  7. Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  8. Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  9. Pascual García M, Bonfill Teixidor E, Planas Rigol E, Rubio Perez C, Iurlaro R, Arias A, et al. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy. Nat Commun. 2019;10:2416 pubmed publisher
  10. Wagner J, Jaurich H, Wallner C, Abraham S, Becerikli M, Dadras M, et al. Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity. J Orthop Res. 2017;35:2425-2434 pubmed publisher
  11. Lavender N, Yang J, Chen S, Sai J, Johnson C, Owens P, et al. The Yin/Yan of CCL2: a minor role in neutrophil anti-tumor activity in vitro but a major role on the outgrowth of metastatic breast cancer lesions in the lung in vivo. BMC Cancer. 2017;17:88 pubmed publisher
  12. Portillo J, Lopez Corcino Y, Miao Y, Tang J, Sheibani N, Kern T, et al. CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes. 2017;66:483-493 pubmed publisher
  13. Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, et al. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A. 2016;113:E71-80 pubmed publisher
  14. Kaplan J, Marshall M, C McSkimming C, Harmon D, Garmey J, Oldham S, et al. Adipocyte progenitor cells initiate monocyte chemoattractant protein-1-mediated macrophage accumulation in visceral adipose tissue. Mol Metab. 2015;4:779-94 pubmed publisher
  15. Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
  16. Chen S, Jiao J, Jiang D, Wan Z, Li L, Li K, et al. T-box transcription factor Brachyury in lung cancer cells inhibits macrophage infiltration by suppressing CCL2 and CCL4 chemokines. Tumour Biol. 2015;36:5881-90 pubmed publisher
  17. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  18. Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher