This is a Validated Antibody Database (VAD) review about mouse Bub3, based on 11 published articles (read how Labome selects the articles), using Bub3 antibody in all methods. It is aimed to help Labome visitors find the most suited Bub3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Bub3 synonym: AU019800; AU021329; AU043350; AW146323; C78067; mitotic checkpoint protein BUB3; WD repeat type I transmembrane protein A72.5; budding uninhibited by benzimidazoles 3 homolog

Santa Cruz Biotechnology
mouse monoclonal (E-7)
  • immunocytochemistry; human
  • western blot; human
Santa Cruz Biotechnology Bub3 antibody (Santa Cruz Biotechnology, sc-376506) was used in immunocytochemistry on human samples and in western blot on human samples . J Biol Chem (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D4F9)
  • western blot; human; loading ...; fig 1c
Cell Signaling Technology Bub3 antibody (Cell Signaling Technology, Ab-8195) was used in western blot on human samples (fig 1c). Cell Cycle (2016) ncbi
BD Biosciences
mouse monoclonal (31/Bub3)
  • ELISA; human; 1:1000; fig 1c
  • western blot; human; 1:1000
BD Biosciences Bub3 antibody (BD Bioscience, 611731) was used in ELISA on human samples at 1:1000 (fig 1c) and in western blot on human samples at 1:1000. MAbs (2016) ncbi
mouse monoclonal (31/Bub3)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Bub3 antibody (BD, 31) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (31/Bub3)
  • western blot; human; 1:500; fig 6
BD Biosciences Bub3 antibody (BD Transduction, 611731) was used in western blot on human samples at 1:500 (fig 6). Nat Commun (2015) ncbi
mouse monoclonal (31/Bub3)
  • immunocytochemistry; human
  • western blot; human
BD Biosciences Bub3 antibody (BD, 611730) was used in immunocytochemistry on human samples and in western blot on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (31/Bub3)
  • western blot; human; fig 1
In order to explore how Tpr regulated chromosomal segregation, BD Biosciences Bub3 antibody (BD Transduction, 611731) was used in western blot on human samples (fig 1). Cell Cycle (2015) ncbi
mouse monoclonal (31/Bub3)
  • immunoprecipitation; human; 1:500; fig s2
BD Biosciences Bub3 antibody (BD Transduction Laboratories, 611730) was used in immunoprecipitation on human samples at 1:500 (fig s2). Nature (2015) ncbi
mouse monoclonal (31/Bub3)
  • western blot; human; fig 3e
In order to report that nuclear pore complexes in interphase cells function as scaffolds for anaphase-inhibitory signaling, BD Biosciences Bub3 antibody (BD, 611731) was used in western blot on human samples (fig 3e). Cell (2014) ncbi
mouse monoclonal (31/Bub3)
  • western blot; human; 1:500
BD Biosciences Bub3 antibody (BD, 611731) was used in western blot on human samples at 1:500. EMBO Rep (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; mouse; loading ...; fig s3b
Sigma-Aldrich Bub3 antibody (Sigma-Aldrich, B7811) was used in western blot on mouse samples (fig s3b). Oncogene (2017) ncbi
rabbit polyclonal
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Sigma-Aldrich Bub3 antibody (SIGMA, B7811) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
Articles Reviewed
  1. Dawar S, Lim Y, Puccini J, White M, Thomas P, Bouchier Hayes L, et al. Caspase-2-mediated cell death is required for deleting aneuploid cells. Oncogene. 2017;36:2704-2714 pubmed publisher
  2. Salsi V, Fantini S, Zappavigna V. NUP98 fusion oncoproteins interact with the APC/C(Cdc20) as a pseudosubstrate and prevent mitotic checkpoint complex binding. Cell Cycle. 2016;15:2275-87 pubmed publisher
  3. Sedgwick G, Larsen M, Lischetti T, Streicher W, Jersie Christensen R, Olsen J, et al. Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling. MAbs. 2016;8:689-97 pubmed publisher
  4. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  5. Zhang G, Lischetti T, Hayward D, Nilsson J. Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint. Nat Commun. 2015;6:7162 pubmed publisher
  6. Scialpi F, Mellis D, Ditzel M. EDD, a ubiquitin-protein ligase of the N-end rule pathway, associates with spindle assembly checkpoint components and regulates the mitotic response to nocodazole. J Biol Chem. 2015;290:12585-94 pubmed publisher
  7. Zhu S, Jing R, Yang Y, Huang Y, Wang X, Leng Y, et al. A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint. J Biol Chem. 2015;290:11282-92 pubmed publisher
  8. Kobayashi A, Hashizume C, Dowaki T, Wong R. Therapeutic potential of mitotic interaction between the nucleoporin Tpr and aurora kinase A. Cell Cycle. 2015;14:1447-58 pubmed publisher
  9. Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  10. Rodriguez Bravo V, Maciejowski J, Corona J, Buch H, Collin P, Kanemaki M, et al. Nuclear pores protect genome integrity by assembling a premitotic and Mad1-dependent anaphase inhibitor. Cell. 2014;156:1017-31 pubmed publisher
  11. Hein J, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. EMBO Rep. 2014;15:264-72 pubmed publisher