This is a Validated Antibody Database (VAD) review about mouse Btk, based on 19 published articles (read how Labome selects the articles), using Btk antibody in all methods. It is aimed to help Labome visitors find the most suited Btk antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Btk synonym: AI528679; xid

Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 5a
Abcam Btk antibody (Abcam, ab25971) was used in western blot knockout validation on mouse samples at 1:1000 (fig 5a). Int J Mol Med (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (7F12H4)
  • immunohistochemistry - paraffin section; human; 1:400; fig 1
  • western blot; human; 1:600; fig 1
Santa Cruz Biotechnology Btk antibody (Santa Cruz, SC-81159) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 1) and in western blot on human samples at 1:600 (fig 1). Oncotarget (2015) ncbi
mouse monoclonal (E-9)
  • immunocytochemistry; human; 1:400; fig 3
  • western blot; human; 1:700; fig 2
Santa Cruz Biotechnology Btk antibody (Santa Cruz, sc-28387) was used in immunocytochemistry on human samples at 1:400 (fig 3) and in western blot on human samples at 1:700 (fig 2). Oncotarget (2015) ncbi
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 5a
Abcam Btk antibody (Abcam, ab25971) was used in western blot knockout validation on mouse samples at 1:1000 (fig 5a). Int J Mol Med (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D3H5)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1b
Cell Signaling Technology Btk antibody (Cell Signaling Technology, 8547) was used in immunohistochemistry on mouse samples at 1:200 (fig 1b). Int J Mol Med (2021) ncbi
domestic rabbit monoclonal (D3H5)
  • mass cytometry; human; 1:200; loading ...; fig s2
Cell Signaling Technology Btk antibody (Cell Signaling, D3H5) was used in mass cytometry on human samples at 1:200 (fig s2). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; human; 1:1000; loading ...; fig 3b, 3e
Cell Signaling Technology Btk antibody (Cell Signaling and Technology, D3H5) was used in western blot on human samples at 1:1000 (fig 3b, 3e). Mol Oncol (2020) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; mouse; loading ...; fig s1a
Cell Signaling Technology Btk antibody (Cell Signaling, 8547S) was used in western blot on mouse samples (fig s1a). Oncogene (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s1a
Cell Signaling Technology Btk antibody (Cell Signaling, 5082S) was used in western blot on mouse samples (fig s1a). Oncogene (2019) ncbi
domestic rabbit monoclonal (D9T6H)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology Btk antibody (Cell Signaling, 87141) was used in western blot on mouse samples (fig 5a). Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6a
Cell Signaling Technology Btk antibody (Cell Signaling, 5082S) was used in western blot on human samples at 1:1000 (fig 6a). Gut (2018) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology Btk antibody (Cell Signaling, 3533) was used in western blot on human samples (fig 3f). Oncotarget (2017) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; human; loading ...; fig 5d
In order to report that therapeutic antibody-dependent tumor cell killing was FcgammaR-dependent and mediated by macrophages, Cell Signaling Technology Btk antibody (Cell Signalling, 8547) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5d
In order to report that therapeutic antibody-dependent tumor cell killing was FcgammaR-dependent and mediated by macrophages, Cell Signaling Technology Btk antibody (Cell Signalling, 5082) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human
Cell Signaling Technology Btk antibody (Cell Signaling, 3533) was used in western blot on human samples . Nature (2016) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; mouse; 1:1000; fig 4
Cell Signaling Technology Btk antibody (Cell Signaling, 3533) was used in western blot on mouse samples at 1:1000 (fig 4). Leukemia (2016) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; human; fig 5b
In order to elucidate how hepatitis C virus infection alters B cell proliferation, Cell Signaling Technology Btk antibody (cell signalling technology, 8547) was used in western blot on human samples (fig 5b). Oncogene (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 5b
In order to elucidate how hepatitis C virus infection alters B cell proliferation, Cell Signaling Technology Btk antibody (cell signalling technology, 5082) was used in western blot on human samples (fig 5b). Oncogene (2016) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology Btk antibody (Cell Signaling Tech, 3533) was used in western blot on human samples at 1:1000 (fig 5). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; rat; fig 5
Cell Signaling Technology Btk antibody (Cell Signaling, 8547) was used in western blot on rat samples (fig 5). Nat Chem Biol (2015) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; mouse; fig 2b
  • western blot; human; fig 1a
Cell Signaling Technology Btk antibody (Cell Signaling, 3533) was used in western blot on mouse samples (fig 2b) and in western blot on human samples (fig 1a). Nature (2015) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; mouse; 1:1000; fig 8c
In order to explore a macrophage TLR9-BTK-calcineurin-NFAT signaling pathway involved in impair fungal immunity, Cell Signaling Technology Btk antibody (Cell signaling, 8547) was used in western blot on mouse samples at 1:1000 (fig 8c). EMBO Mol Med (2015) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; mouse
Cell Signaling Technology Btk antibody (Cell Signaling Technology, 3533) was used in western blot on mouse samples . Molecules (2014) ncbi
BD Biosciences
mouse monoclonal (N35-86)
  • flow cytometry; mouse; loading ...; fig 1f
BD Biosciences Btk antibody (BD Biosciences, N35-86) was used in flow cytometry on mouse samples (fig 1f). Front Immunol (2018) ncbi
mouse monoclonal (N35-86)
  • flow cytometry; human; loading ...; fig 1b
BD Biosciences Btk antibody (BD, 562753) was used in flow cytometry on human samples (fig 1b). J Exp Med (2018) ncbi
Articles Reviewed
  1. Zhao J, Chen J, Li Y, Xia L, Wu Y. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48: pubmed publisher
  2. West J, Austin E, Rizzi E, Yan L, Tanjore H, Crabtree A, et al. KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models. Int J Mol Sci. 2021;22: pubmed publisher
  3. Thomsen E, Rovsing A, Anderson M, Due H, Huang J, Luo Y, et al. Identification of BLNK and BTK as mediators of rituximab-induced programmed cell death by CRISPR screens in GCB-subtype diffuse large B-cell lymphoma. Mol Oncol. 2020;: pubmed publisher
  4. Das S, Bar Sagi D. BTK signaling drives CD1dhiCD5+ regulatory B-cell differentiation to promote pancreatic carcinogenesis. Oncogene. 2019;38:3316-3324 pubmed publisher
  5. Xu X, Xu J, Wu J, Hu Y, Han Y, Gu Y, et al. Phosphorylation-Mediated IFN-γR2 Membrane Translocation Is Required to Activate Macrophage Innate Response. Cell. 2018;175:1336-1351.e17 pubmed publisher
  6. Hwang I, Boularan C, Harrison K, Kehrl J. Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol. 2018;9:687 pubmed publisher
  7. Tissino E, Benedetti D, Herman S, ten Hacken E, Ahn I, Chaffee K, et al. Functional and clinical relevance of VLA-4 (CD49d/CD29) in ibrutinib-treated chronic lymphocytic leukemia. J Exp Med. 2018;215:681-697 pubmed publisher
  8. Chong I, Aronson L, Bryant H, Gulati A, Campbell J, Elliott R, et al. Mapping genetic vulnerabilities reveals BTK as a novel therapeutic target in oesophageal cancer. Gut. 2018;67:1780-1792 pubmed publisher
  9. Mondello P, Derenzini E, Asgari Z, Philip J, Brea E, SESHAN V, et al. Dual inhibition of histone deacetylases and phosphoinositide 3-kinase enhances therapeutic activity against B cell lymphoma. Oncotarget. 2017;8:14017-14028 pubmed publisher
  10. Burgess M, Mapp S, Mazzieri R, Cheung C, Chambers L, Mattarollo S, et al. Increased FcγRIIB dominance contributes to the emergence of resistance to therapeutic antibodies in chronic lymphocytic leukaemia patients. Oncogene. 2017;36:2366-2376 pubmed publisher
  11. He Y, Zeng M, Yang D, Motro B, Núñez G. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. 2016;530:354-7 pubmed publisher
  12. Chen S, Chang B, Chang S, Tong T, Ham S, Sherry B, et al. BTK inhibition results in impaired CXCR4 chemokine receptor surface expression, signaling and function in chronic lymphocytic leukemia. Leukemia. 2016;30:833-43 pubmed publisher
  13. Dai B, Chen A, Corkum C, Peroutka R, Landon A, Houng S, et al. Hepatitis C virus upregulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders. Oncogene. 2016;35:2979-90 pubmed publisher
  14. Lorkova L, Scigelova M, Arrey T, Vit O, Pospisilova J, Doktorova E, et al. Detailed Functional and Proteomic Characterization of Fludarabine Resistance in Mantle Cell Lymphoma Cells. PLoS ONE. 2015;10:e0135314 pubmed publisher
  15. Zucha M, Wu A, Lee W, Wang L, Lin W, Yuan C, et al. Bruton's tyrosine kinase (Btk) inhibitor ibrutinib suppresses stem-like traits in ovarian cancer. Oncotarget. 2015;6:13255-68 pubmed
  16. Bradshaw J, McFarland J, Paavilainen V, Bisconte A, Tam D, Phan V, et al. Prolonged and tunable residence time using reversible covalent kinase inhibitors. Nat Chem Biol. 2015;11:525-31 pubmed publisher
  17. Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher
  18. Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher
  19. Baek J, Kim J, Cheon Y, Park S, Ahn S, Yoon K, et al. Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone. Molecules. 2014;19:11628-44 pubmed publisher