This is a Validated Antibody Database (VAD) review about mouse Brd4, based on 12 published articles (read how Labome selects the articles), using Brd4 antibody in all methods. It is aimed to help Labome visitors find the most suited Brd4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Brd4 synonym: Brd5; HUNK1; MCAP; WI-11513; bromodomain-containing protein 4; bromodomain-containing 5; fsh-like protein; mitotic chromosome-associated protein

Abcam
rabbit monoclonal (EPR5150(2))
  • western blot; human; fig 1c
Abcam Brd4 antibody (Abcam, ab128874) was used in western blot on human samples (fig 1c). Cell Res (2018) ncbi
rabbit monoclonal (EPR5150(2))
  • immunoprecipitation; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 4a
Abcam Brd4 antibody (Abcam, ab128874) was used in immunoprecipitation on human samples (fig 1b) and in western blot on human samples (fig 4a). Nat Med (2017) ncbi
rabbit monoclonal (EPR5150(2))
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 6d
  • western blot; mouse; 1:1000; loading ...; fig 7b
Abcam Brd4 antibody (Abcam, 128874) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 6d) and in western blot on mouse samples at 1:1000 (fig 7b). J Neuroinflammation (2017) ncbi
rabbit monoclonal (EPR5150(2))
  • immunoprecipitation; human; loading ...; fig 4b
  • immunocytochemistry; human; 1:500; loading ...; fig 5b
  • western blot; human; loading ...; fig 3a
Abcam Brd4 antibody (Abcam, ab128874) was used in immunoprecipitation on human samples (fig 4b), in immunocytochemistry on human samples at 1:500 (fig 5b) and in western blot on human samples (fig 3a). PLoS Pathog (2016) ncbi
rabbit monoclonal (EPR5150(2))
  • immunohistochemistry; human; 1:100; fig s2
  • western blot; human; 1:1000; fig s1
Abcam Brd4 antibody (Abcam, ab128874) was used in immunohistochemistry on human samples at 1:100 (fig s2) and in western blot on human samples at 1:1000 (fig s1). Mol Cancer Ther (2016) ncbi
rabbit monoclonal (EPR5150(2))
  • ChIP-Seq; mouse; fig 5
  • western blot; mouse; fig 3b
Abcam Brd4 antibody (Abcam, 128874) was used in ChIP-Seq on mouse samples (fig 5) and in western blot on mouse samples (fig 3b). BMC Genomics (2016) ncbi
rabbit monoclonal (EPR5150(2))
  • immunocytochemistry; human; 1:200; fig 4
Abcam Brd4 antibody (Abcam, ab128874) was used in immunocytochemistry on human samples at 1:200 (fig 4). Epigenetics Chromatin (2015) ncbi
rabbit monoclonal (EPR5150(2))
  • western blot; mouse; fig s1f
Abcam Brd4 antibody (abcam, ab128874) was used in western blot on mouse samples (fig s1f). Nature (2015) ncbi
rabbit monoclonal (EPR5150(2))
  • western blot; human; fig 3
In order to assess reduction of c-MYC in acute leukemia cells due to a BET inhibitor OTX015 that targets BRD2 and BRD4, Abcam Brd4 antibody (Epitomics, 5716-1) was used in western blot on human samples (fig 3). Oncotarget (2015) ncbi
rabbit monoclonal (EPR5150(2))
  • western blot; human; 1:5000; fig s1
Abcam Brd4 antibody (Epitomics, 5716-1) was used in western blot on human samples at 1:5000 (fig s1). Cell Rep (2014) ncbi
rabbit monoclonal (EPR5150(2))
  • western blot; mouse
Abcam Brd4 antibody (Abcam, ab128874) was used in western blot on mouse samples . In Vivo (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam Brd4 antibody (Abcam, ab75898) was used in western blot on human samples . J Biol Chem (2014) ncbi
Articles Reviewed
  1. Yu F, Shi G, Cheng S, Chen J, Wu S, Wang Z, et al. SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation. Cell Res. 2018;28:670-685 pubmed publisher
  2. Zhang P, Wang D, Zhao Y, Ren S, Gao K, Ye Z, et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med. 2017;23:1055-1062 pubmed publisher
  3. Zhao L, Li J, Fu Y, Zhang M, Wang B, Ouellette J, et al. Photoreceptor protection via blockade of BET epigenetic readers in a murine model of inherited retinal degeneration. J Neuroinflammation. 2017;14:14 pubmed publisher
  4. Ren K, Zhang W, Chen X, Ma Y, Dai Y, Fan Y, et al. An Epigenetic Compound Library Screen Identifies BET Inhibitors That Promote HSV-1 and -2 Replication by Bridging P-TEFb to Viral Gene Promoters through BRD4. PLoS Pathog. 2016;12:e1005950 pubmed publisher
  5. Tögel L, Nightingale R, Chueh A, Jayachandran A, Tran H, Phesse T, et al. Dual Targeting of Bromodomain and Extraterminal Domain Proteins, and WNT or MAPK Signaling, Inhibits c-MYC Expression and Proliferation of Colorectal Cancer Cells. Mol Cancer Ther. 2016;15:1217-26 pubmed publisher
  6. Gonzales Cope M, Sidoli S, Bhanu N, Won K, Garcia B. Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells. BMC Genomics. 2016;17:95 pubmed publisher
  7. Zhan Y, Kost Alimova M, Shi X, Leo E, Bardenhagen J, Shepard H, et al. Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery. Epigenetics Chromatin. 2015;8:37 pubmed publisher
  8. Fong C, Gilan O, Lam E, Rubin A, Ftouni S, Tyler D, et al. BET inhibitor resistance emerges from leukaemia stem cells. Nature. 2015;525:538-42 pubmed publisher
  9. Coudé M, Braun T, Berrou J, Dupont M, Bertrand S, Massé A, et al. BET inhibitor OTX015 targets BRD2 and BRD4 and decreases c-MYC in acute leukemia cells. Oncotarget. 2015;6:17698-712 pubmed
  10. Nagarajan S, Hossan T, Alawi M, Najafova Z, Indenbirken D, Bedi U, et al. Bromodomain protein BRD4 is required for estrogen receptor-dependent enhancer activation and gene transcription. Cell Rep. 2014;8:460-9 pubmed publisher
  11. Kalash R, Berhane H, Au J, Rhieu B, Epperly M, Goff J, et al. Differences in irradiated lung gene transcription between fibrosis-prone C57BL/6NHsd and fibrosis-resistant C3H/HeNHsd mice. In Vivo. 2014;28:147-71 pubmed
  12. Liu P, Xiang Y, Fujinaga K, Bartholomeeusen K, Nilson K, Price D, et al. Release of positive transcription elongation factor b (P-TEFb) from 7SK small nuclear ribonucleoprotein (snRNP) activates hexamethylene bisacetamide-inducible protein (HEXIM1) transcription. J Biol Chem. 2014;289:9918-25 pubmed publisher