This is a Validated Antibody Database (VAD) review about mouse Braf, based on 36 published articles (read how Labome selects the articles), using Braf antibody in all methods. It is aimed to help Labome visitors find the most suited Braf antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Braf synonym: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; Braf-2; Braf2; C230098H17; C87398; D6Ertd631e

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (F-7)
  • western blot; human; 1:1000; fig 9
  • western blot knockout validation; mouse; 1:1000; fig 3
In order to study prevention of allergic skin disease by epidermal RAF, Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (fig 9) and in western blot knockout validation on mouse samples at 1:1000 (fig 3). elife (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-7)
  • western blot knockout validation; mouse; 1:500; fig 4b
Santa Cruz Biotechnology Braf antibody (Santa, sc-5284) was used in western blot knockout validation on mouse samples at 1:500 (fig 4b). Cell Signal (2013) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (55C6)
  • western blot knockout validation; mouse; 1:500; fig 3b
Cell Signaling Technology Braf antibody (Cell Signaling, 9433) was used in western blot knockout validation on mouse samples at 1:500 (fig 3b). Nat Commun (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-7)
  • western blot; human; fig s2b
Santa Cruz Biotechnology Braf antibody (Santa Cruz Biotechnology, sc-5284) was used in western blot on human samples (fig s2b). J Cell Biol (2021) ncbi
mouse monoclonal (F-7)
  • western blot; human; 1:3000; loading ...; fig 2c
Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:3000 (fig 2c). Nat Commun (2021) ncbi
mouse monoclonal (F-7)
  • western blot; human; 1:1000; fig 1a
Santa Cruz Biotechnology Braf antibody (Santa Cruz Biotechnology, sc-5284) was used in western blot on human samples at 1:1000 (fig 1a). Aging (Albany NY) (2021) ncbi
mouse monoclonal (F-3)
  • western blot; human; 1:1000; loading ...; fig ex1m
Santa Cruz Biotechnology Braf antibody (Santa, sc-55522) was used in western blot on human samples at 1:1000 (fig ex1m). Nature (2018) ncbi
mouse monoclonal (F-7)
  • western blot; human; 1:1000; loading ...; fig 5c
Santa Cruz Biotechnology Braf antibody (SantaCruz, sc-5284) was used in western blot on human samples at 1:1000 (fig 5c). Nat Commun (2017) ncbi
mouse monoclonal (F-7)
  • western blot; human; loading ...; fig 3e
In order to show that PHB1 is highly expressed in non-small cell lung cancers patients and correlates with poor survival, Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig 3e). Oncogene (2017) ncbi
mouse monoclonal (F-7)
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
mouse monoclonal (F-7)
  • reverse phase protein lysate microarray; human; loading ...; fig 3a
In order to describe the features of 228 primary cervical cancers, Santa Cruz Biotechnology Braf antibody (SantaCruz, sc-5284) was used in reverse phase protein lysate microarray on human samples (fig 3a). Nature (2017) ncbi
mouse monoclonal (F-7)
  • western blot; human; loading ...; fig 2c
Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig 2c). Oncogene (2017) ncbi
mouse monoclonal (F-7)
  • proximity ligation assay; hamsters; loading ...; fig 3d
In order to clarify the interaction of SPRED1 with Ras and Raf, Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in proximity ligation assay on hamsters samples (fig 3d). Mol Cell Biol (2016) ncbi
mouse monoclonal (F-7)
  • western blot; human; 1:1000; fig 9
  • western blot knockout validation; mouse; 1:1000; fig 3
In order to study prevention of allergic skin disease by epidermal RAF, Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (fig 9) and in western blot knockout validation on mouse samples at 1:1000 (fig 3). elife (2016) ncbi
mouse monoclonal (F-7)
  • western blot; human; fig 6b
In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Santa Cruz Biotechnology Braf antibody (Santa Cruz, F7) was used in western blot on human samples (fig 6b). Cell Rep (2016) ncbi
mouse monoclonal
  • western blot; human; fig 6b
In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Santa Cruz Biotechnology Braf antibody (Santa Cruz, F7) was used in western blot on human samples (fig 6b). Cell Rep (2016) ncbi
mouse monoclonal (F-7)
  • western blot; human; 1:1000; fig s1
Santa Cruz Biotechnology Braf antibody (santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (fig s1). Sci Rep (2016) ncbi
mouse monoclonal (F-7)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
In order to determine limits on FAK-dependent invasion and enhancement of the response to melanoma treatment with BRAF inhibitors due to HSP70 inhibition, Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). Cancer Res (2016) ncbi
mouse monoclonal (F-7)
  • western blot; human; 1:1000; tbl 2
Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (tbl 2). elife (2016) ncbi
mouse monoclonal (F-7)
  • western blot; human; fig 1
Santa Cruz Biotechnology Braf antibody (santa cruz, sc-5284) was used in western blot on human samples (fig 1). Carcinogenesis (2015) ncbi
mouse monoclonal (F-7)
  • western blot; human; loading ...; fig S3A
Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig S3A). Autophagy (2015) ncbi
mouse monoclonal (F-7)
  • western blot; human; fig 3b
Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig 3b). Oncotarget (2015) ncbi
mouse monoclonal (F-7)
  • immunoprecipitation; human; 1:1000; fig 2
  • immunocytochemistry; human; 1:25; fig 5
Santa Cruz Biotechnology Braf antibody (Santa Cruz, sc-5284) was used in immunoprecipitation on human samples at 1:1000 (fig 2) and in immunocytochemistry on human samples at 1:25 (fig 5). Oncogene (2016) ncbi
mouse monoclonal (F-3)
  • western blot; mouse; 1:200; fig 2
In order to investigate how BRAF/MAPK activity regulates intestinal stem cell populations and contributes to colon cancer, Santa Cruz Biotechnology Braf antibody (Santa Cruz, 55522) was used in western blot on mouse samples at 1:200 (fig 2). Oncogene (2015) ncbi
mouse monoclonal (F-7)
  • western blot; human
Santa Cruz Biotechnology Braf antibody (Santa Cruz Biotechnology, sc-5284) was used in western blot on human samples . Cancer Res (2014) ncbi
mouse monoclonal (F-7)
  • western blot knockout validation; mouse; 1:500; fig 4b
Santa Cruz Biotechnology Braf antibody (Santa, sc-5284) was used in western blot knockout validation on mouse samples at 1:500 (fig 4b). Cell Signal (2013) ncbi
mouse monoclonal (F-7)
  • western blot; hamsters
Santa Cruz Biotechnology Braf antibody (Santa Cruz Biotechnology, sc-5284) was used in western blot on hamsters samples . Mol Cell Biol (2013) ncbi
Abcam
domestic rabbit monoclonal (EPR2207)
  • western blot; mouse; 1:1000; fig 2c
In order to identify specific genes involved in leukemogenesis through comprehensive ex vivo transposon mutagenesis, Abcam Braf antibody (Abcam, EPR2207) was used in western blot on mouse samples at 1:1000 (fig 2c). Cancer Res (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5g
Cell Signaling Technology Braf antibody (Cell Signaling Technology, 2696) was used in western blot on human samples (fig 5g). Int J Biol Sci (2022) ncbi
domestic rabbit monoclonal (55C6)
  • western blot knockout validation; mouse; 1:500; fig 3b
Cell Signaling Technology Braf antibody (Cell Signaling, 9433) was used in western blot knockout validation on mouse samples at 1:500 (fig 3b). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology Braf antibody (Cell Signaling, 2696) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1c
In order to assess the effects of LY3009120, a panRAF and RAF dimer inhibitor, in human models of colorectal cancer, Cell Signaling Technology Braf antibody (Cell Signaling, 2696) was used in western blot on human samples (fig 1c). Oncotarget (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:100; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology Braf antibody (Cell Signaling, 2696) was used in western blot on human samples at 1:100 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (55C6)
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
  • western blot; human; 1:1000
In order to elucidate a screening that identifies kinases involved in prostate cancer visceral and bone metastasis, Cell Signaling Technology Braf antibody (Cell Signaling Technology, 55C6) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4) and in western blot on human samples at 1:1000. Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 3c
Cell Signaling Technology Braf antibody (Cell Signaling, 2696) was used in western blot on human samples at 1:500 (fig 3c). Am J Physiol Lung Cell Mol Physiol (2016) ncbi
domestic rabbit monoclonal (55C6)
  • western blot; human; fig 2e
Cell Signaling Technology Braf antibody (Cell Signaling Technology, 9433) was used in western blot on human samples (fig 2e). Oncogene (2016) ncbi
domestic rabbit monoclonal (55C6)
  • western blot; human; 1:1000; fig 5a
Cell Signaling Technology Braf antibody (Cell Signaling Technology, 9433) was used in western blot on human samples at 1:1000 (fig 5a). Cancer Cell Int (2015) ncbi
domestic rabbit monoclonal (55C6)
  • western blot; human; 1:1000
In order to test if NBDHEX and MC3181 have antitumor activity against melanoma cells resistant to vemurafenib, Cell Signaling Technology Braf antibody (Cell Signaling Technology, 9433) was used in western blot on human samples at 1:1000. Biochem Pharmacol (2015) ncbi
BD Biosciences
mouse monoclonal (13/B-RAF)
  • immunoprecipitation; mouse; loading ...; fig 4s1b, 4s1d
BD Biosciences Braf antibody (BD, 612375) was used in immunoprecipitation on mouse samples (fig 4s1b, 4s1d). elife (2016) ncbi
mouse monoclonal (13/B-RAF)
  • western blot; human; 1:5000; loading ...; fig 1a
In order to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase, BD Biosciences Braf antibody (BD Biosciences, 612375) was used in western blot on human samples at 1:5000 (fig 1a). J Biol Chem (2016) ncbi
Articles Reviewed
  1. Wang M, Zhang C, Zheng Q, Ma Z, Qi M, Di G, et al. RhoJ facilitates angiogenesis in glioblastoma via JNK/VEGFR2 mediated activation of PAK and ERK signaling pathways. Int J Biol Sci. 2022;18:942-955 pubmed publisher
  2. Leon K, Buj R, Lesko E, Dahl E, Chen C, Tangudu N, et al. DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A. J Cell Biol. 2021;220: pubmed publisher
  3. Gualtieri A, Kyprianou N, Gregory L, Vignola M, Nicholson J, Tan R, et al. Activating mutations in BRAF disrupt the hypothalamo-pituitary axis leading to hypopituitarism in mice and humans. Nat Commun. 2021;12:2028 pubmed publisher
  4. Ischenko I, D Amico S, Rao M, Li J, Hayman M, Powers S, et al. KRAS drives immune evasion in a genetic model of pancreatic cancer. Nat Commun. 2021;12:1482 pubmed publisher
  5. Buj R, Leon K, Anguelov M, Aird K. Suppression of p16 alleviates the senescence-associated secretory phenotype. Aging (Albany NY). 2021;13:3290-3312 pubmed publisher
  6. Rapino F, Delaunay S, Rambow F, Zhou Z, Tharun L, de Tullio P, et al. Codon-specific translation reprogramming promotes resistance to targeted therapy. Nature. 2018;558:605-609 pubmed publisher
  7. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  8. Schwartz J, Ma J, Lamprecht T, Walsh M, Wang S, Bryant V, et al. The genomic landscape of pediatric myelodysplastic syndromes. Nat Commun. 2017;8:1557 pubmed publisher
  9. Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, et al. Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours. Oncogene. 2017;36:4778-4789 pubmed publisher
  10. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  11. . Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
  12. Riverso M, Montagnani V, Stecca B. KLF4 is regulated by RAS/RAF/MEK/ERK signaling through E2F1 and promotes melanoma cell growth. Oncogene. 2017;36:3322-3333 pubmed publisher
  13. Vakana E, Pratt S, Blosser W, Dowless M, Simpson N, Yuan X, et al. LY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer. Oncotarget. 2017;8:9251-9266 pubmed publisher
  14. Shin J, Watanabe S, Hoelper S, Kruger M, Kostin S, Pöling J, et al. BRAF activates PAX3 to control muscle precursor cell migration during forelimb muscle development. elife. 2016;5: pubmed publisher
  15. Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
  16. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  17. Siljamäki E, Abankwa D. SPRED1 Interferes with K-ras but Not H-ras Membrane Anchorage and Signaling. Mol Cell Biol. 2016;36:2612-25 pubmed publisher
  18. Raguz J, Jerić I, Niault T, Nowacka J, Kuzet S, Rupp C, et al. Epidermal RAF prevents allergic skin disease. elife. 2016;5: pubmed publisher
  19. Kemper K, Krijgsman O, Kong X, Cornelissen Steijger P, Shahrabi A, Weeber F, et al. BRAF(V600E) Kinase Domain Duplication Identified in Therapy-Refractory Melanoma Patient-Derived Xenografts. Cell Rep. 2016;16:263-277 pubmed publisher
  20. Chatelle C, Hövermann D, Muller A, Wagner H, Weber W, Radziwill G. Optogenetically controlled RAF to characterize BRAF and CRAF protein kinase inhibitors. Sci Rep. 2016;6:23713 pubmed publisher
  21. Budina Kolomets A, Webster M, Leu J, Jennis M, Krepler C, Guerrini A, et al. HSP70 Inhibition Limits FAK-Dependent Invasion and Enhances the Response to Melanoma Treatment with BRAF Inhibitors. Cancer Res. 2016;76:2720-30 pubmed publisher
  22. Bhargava A, Pelech S, Woodard B, Kerwin J, Maherali N. Registered report: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. elife. 2016;5: pubmed publisher
  23. Guo Y, Updegraff B, Park S, Durakoglugil D, Cruz V, Maddux S, et al. Comprehensive Ex Vivo Transposon Mutagenesis Identifies Genes That Promote Growth Factor Independence and Leukemogenesis. Cancer Res. 2016;76:773-86 pubmed publisher
  24. Faltermeier C, Drake J, Clark P, Smith B, Zong Y, Volpe C, et al. Functional screen identifies kinases driving prostate cancer visceral and bone metastasis. Proc Natl Acad Sci U S A. 2016;113:E172-81 pubmed publisher
  25. Awad K, Elinoff J, Wang S, Gairhe S, Ferreyra G, Cai R, et al. Raf/ERK drives the proliferative and invasive phenotype of BMPR2-silenced pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2016;310:L187-201 pubmed publisher
  26. Lenain C, Gusyatiner O, Douma S, van den Broek B, Peeper D. Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence. Carcinogenesis. 2015;36:1263-74 pubmed publisher
  27. Wiersma V, de Bruyn M, Wei Y, van Ginkel R, Hirashima M, Niki T, et al. The epithelial polarity regulator LGALS9/galectin-9 induces fatal frustrated autophagy in KRAS mutant colon carcinoma that depends on elevated basal autophagic flux. Autophagy. 2015;11:1373-88 pubmed publisher
  28. Condelli V, Maddalena F, Sisinni L, Lettini G, Matassa D, Piscazzi A, et al. Targeting TRAP1 as a downstream effector of BRAF cytoprotective pathway: a novel strategy for human BRAF-driven colorectal carcinoma. Oncotarget. 2015;6:22298-309 pubmed
  29. Fedorenko I, Abel E, Koomen J, Fang B, Wood E, Chen Y, et al. Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene. 2016;35:1225-35 pubmed publisher
  30. Ho J, Nadeem A, Rydström A, Puthia M, Svanborg C. Targeting of nucleotide-binding proteins by HAMLET--a conserved tumor cell death mechanism. Oncogene. 2016;35:897-907 pubmed publisher
  31. Wang Z, Ma B, Ji X, Deng Y, Zhang T, Zhang X, et al. MicroRNA-378-5p suppresses cell proliferation and induces apoptosis in colorectal cancer cells by targeting BRAF. Cancer Cell Int. 2015;15:40 pubmed publisher
  32. Graziani G, Artuso S, De Luca A, Muzi A, Rotili D, Scimeca M, et al. A new water soluble MAPK activator exerts antitumor activity in melanoma cells resistant to the BRAF inhibitor vemurafenib. Biochem Pharmacol. 2015;95:16-27 pubmed publisher
  33. Riemer P, Sreekumar A, Reinke S, Rad R, Schäfer R, Sers C, et al. Transgenic expression of oncogenic BRAF induces loss of stem cells in the mouse intestine, which is antagonized by β-catenin activity. Oncogene. 2015;34:3164-75 pubmed publisher
  34. Kugel C, Hartsough E, Davies M, Setiady Y, Aplin A. Function-blocking ERBB3 antibody inhibits the adaptive response to RAF inhibitor. Cancer Res. 2014;74:4122-32 pubmed publisher
  35. Zeng L, Ehrenreiter K, Menon J, Menard R, Kern F, Nakazawa Y, et al. RKIP regulates MAP kinase signaling in cells with defective B-Raf activity. Cell Signal. 2013;25:1156-65 pubmed publisher
  36. van der Hoeven D, Cho K, Ma X, Chigurupati S, Parton R, Hancock J. Fendiline inhibits K-Ras plasma membrane localization and blocks K-Ras signal transmission. Mol Cell Biol. 2013;33:237-51 pubmed publisher